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Citations for Becton, Dickinson and Company :
1651 - 1700 of 8332 citations for 6 Chloro 2 methyl 1 2 4 triazolo 4 3 b pyridazin 3 2H one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: The following antibodies were used: IFNγ-PE-Cy7 and IL-4-PE (BD Biosciences, San Jose, CA). Cells were fixed in 2% paraformaldehyde and permeablized with 0.1% saponin in PBS to detect intracellular antigens ...
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bioRxiv - Immunology 2019Quote: ... Cells were stained in sterile PBS for 30min at 4°C with anti-CD8α (RPA-T8, BD) and Near-IR live-dead marker (ThermoFisher) ...
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bioRxiv - Immunology 2019Quote: ... cells were fixed using 4% PFA and permeabilized using Cytofix/Cytoperm solution (BD Biosciences, San Jose, CA). Cells were then incubated with anti-IFN-γ (Cat ...
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bioRxiv - Cell Biology 2020Quote: ... the cells were kept at 4°C before sorting using a FACS Aria Fusion flow sorter (BD). Single cells and MK pools of 20 cells were sorted by FACS according to ploidy level using a 100uM nozzle directly into individual wells of a 96-well plate.
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bioRxiv - Immunology 2020Quote: ... IL-4 (11B11) or IL-10 (JESS-16E3) according to the manufacturer’s instructions (BD Bioscience or eBioscience). At least 3 × 104 live cells ...
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bioRxiv - Immunology 2020Quote: ... iBMDMs (4×104/ml) were seeded in triplicate in black microtest Optilux 96-well plates (BD Falcon) in 1% FCS/DMEM and L-glutamine media for 4 h and then replaced with phenol-red free 1% FCS/DMEM and L-glutamine 20 h prior to 30 mins pretreatment or not with kinase inhibitors (500nM) ...
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bioRxiv - Immunology 2022Quote: ... cells were fixed for 20 min at 4°C using the FOXP3 Fix/Perm kit (BD Biosciences), and washed in permeabilization buffer ...
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bioRxiv - Bioengineering 2023Quote: ... angled blade (Integra Miltex #4-111) and blood was collected in K2 EDTA-coated tubes (BD #367841) via cardiac puncture and samples were stored appropriately for further analysis ...
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bioRxiv - Immunology 2024Quote: ... washed and fixed for 10 minutes at 4°C with fixation/permeabilization solution (BD Cytofix/Cytoperm kit) and washed with permeabilization buffer overnight before staining for 30 minutes with anti-TNF-α ...
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bioRxiv - Pathology 2023Quote: ... followed by incubation for an additional 4 h in the presence GolgiPlug (BD Biosciences, cat. No. 555029) and GolgiStop (BD Biosciences ...
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bioRxiv - Microbiology 2023Quote: ... fixed with 4% PFA and GFP-positive cells were analyzed using BD FACSCelesta flow cytometer (BD Biosciences).
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bioRxiv - Immunology 2023Quote: All samples were resuspended in 4 ml of RBC lysing solution (BD FACS Lysing Solution, BD Biosciences), vortexed and incubated for 15 min at RT protected from light ...
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bioRxiv - Cell Biology 2023Quote: ... ST-HSC and MPP3-4 was performed using a BD FACSAria™ III Cell Sorter (BD Biosciences) using DIVA software (BD Biosciences).
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bioRxiv - Immunology 2023Quote: ... macrophages were plated at 4 × 105 cells/well in 24-well tissue culture-treated plates (BD Falcon), or at 3 × 105 in 24-well non-tissue culture-treated plates ...
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bioRxiv - Immunology 2023Quote: All samples were resuspended in 4 ml of RBC lysing solution (BD FACS Lysing Solution, BD Biosciences), vortexed and incubated for 15 min at RT protected from light ...
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bioRxiv - Neuroscience 2023Quote: ... A single dose at 4 mg/kg bodyweight of the anti-Ly6G antibody (BD PharMingen, Cat# 551459) and isotope control antibody rat IgG2a (ThermoFisher ...
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bioRxiv - Bioengineering 2023Quote: ... the cells were fixed with 4% PFA prior to analysis on flow cytometer (LSR Fortessa, BD BioSciences).
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bioRxiv - Immunology 2024Quote: ... washed twice and then fixed for 5 minutes at 4°C with CytoFix/CytoPerm Buffer (BD Biosciences). The following surface antibodies were used at the manufacturer recommended dilution of 0.25 μg antibody per million cells with the exception of CD57 APC which was tittered down 1/5 in order to be on scale ...
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bioRxiv - Bioengineering 2024Quote: ... the cells were fixed with 4% PFA before analysis on a flow cytometer (LSR Fortessa, BD BioSciences).
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bioRxiv - Bioengineering 2024Quote: ... the cells were fixed with 4% PFA before analysis on a flow cytometer (LSR Fortessa, BD BioSciences).
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bioRxiv - Synthetic Biology 2024Quote: ... Sorting was carried out at 4°C using a BD FACSAria Fusion (BD FACSAria Fusion, BD Biosciences) by specialized facility staff ...
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bioRxiv - Synthetic Biology 2024Quote: ... Sorting was carried out at 4°C using a BD FACSAria Fusion (BD FACSAria Fusion, BD Biosciences) by specialized facility staff ...
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bioRxiv - Neuroscience 2024Quote: ... The stained cell samples were analysed using a FACS LSR Fortessa (4 lasers) flow cytometer (BD Biosciences), and the data were processed using FlowJo software.
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bioRxiv - Systems Biology 2022Quote: ... coli strain JM109 (1 × 1010 colony-forming units (CFU)/ml) were cultured for various times in 2 ml of 2xYT liquid medium (BD Difco, Cat# 244020) containing various concentrations of pyridoxal 5’ -phosphate (pH adjusted to 7.0) ...
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Multiplexed single-cell transcriptomic analysis of normal and impaired lung development in the mousebioRxiv - Cell Biology 2019Quote: ... Cells were incubated in the dark with 2 μl/1×106 cells of CD16/32 antibody (Fc block; BD Biosciences, Mississauga, ON, Canada) for 15 minutes at RT ...
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bioRxiv - Immunology 2022Quote: ... blood cells were stained in 50 µl 2% FCS RPMI medium with antibodies against CD11C (APC, clone HC3, BD biosciences, dilution 1/100), CD11b (PercPCy5.5 or Pacific Blue ...
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bioRxiv - Genomics 2020Quote: ... Granzyme B-PE-CF594 (GB11, BD, 562462), FoxP3-APC (236A/E7 ...
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bioRxiv - Synthetic Biology 2020Quote: ... and remaining reagents from BD OptEIA Reagent Set B (BD Biosciences, 550534). ELISAs were performed with 96-well MaxiSorp plates (Thermo Scientific ...
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bioRxiv - Immunology 2020Quote: ... CD11c (clone B-ly6, BD, BB700, 624381), for myeloid and CD123 (clone 9F5 ...
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bioRxiv - Developmental Biology 2019Quote: ... mouse anti-Aurora B (AIM1, BD Transduction), rabbit anti-Olig2 (Millipore) ...
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bioRxiv - Immunology 2021Quote: ... GC B-cells were sorted by BD FACS Aria III (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... hCD11c (BD Pharmingen, #560999, clone B-ly6), hCD86 (BioLegend ...
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bioRxiv - Cancer Biology 2024Quote: ... and granzyme B (clone GB11; BD Biosciences) antibodies for 30 min at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... HA-KCTD5 and b-tubulin were detected with anti-pan Gb (1/2000, #610288, BD Transduction laboratories), anti-HA (1/2000 ...
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bioRxiv - Microbiology 2021Quote: ... 0.5–1 million cells were stained with one of the following primary antibodies: mouse anti-PSGL1 antibody (KPL-1) (BD Pharmingen), mouse anti-CD43 antibody (1G10 ...
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bioRxiv - Cell Biology 2020Quote: ... hepatocyte organoids were similarly electroporated and 3 days after electroporation cells were recovered from the BMER and used for FACS analysis (FACS Aria, BD). DAPI was used to identify live cells ...
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bioRxiv - Systems Biology 2019Quote: Total T cells were isolated from peripheral blood mononuclear cells from 3 human donors via negative selection (Stem Cell Technologie cat. no. 19051) and plated in anti-CD3 (BD Pharmingen clone UCHT1 ...
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bioRxiv - Immunology 2021Quote: ... resuspended in 200 μL wash buffer and acquired on a high-throughput sampler with a 3-laser FACSCanto™ (BD). All incubation steps were performed under 600 rpm on an orbital shaker at room temperature and protected from light ...
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bioRxiv - Bioengineering 2021Quote: Six milliliters of blood were collected via venipuncture from five healthy donors and equally divided between two 3 mL tubes of ethylenediaminetetraacetic acid (EDTA) (BD Medical 367856 Vacutainer® ...
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bioRxiv - Bioengineering 2020Quote: ... During this process, we employed a 3-axis precision linear stage (461-XYZ-M, Newport) to position a thin needle (21G, BD) connected to a vacuum pump to remove the tissue inside the capillary ...
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bioRxiv - Cancer Biology 2019Quote: ... three-dimensional cultures (3-D) were prepared with 2.5 × 105 cells seeded in MW6 plates covered by a layer of matrigel (BD, NJ). When indicated ...
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bioRxiv - Microbiology 2021Quote: To assess the effect of Pf3D7_1437000 depletion on parasite growth parasites were maintained in technical triplicate (3 × 1mL culture) and their growth monitored daily by flow cytometry (BD FACSCanto with attached High Throughput Sampler ...
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bioRxiv - Immunology 2021Quote: ... From each sample 3 μL were added to a volume of 47 μL of reagents for PCR3 as described by BD’s user guide following the protocol and number of cycles listed ...
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bioRxiv - Immunology 2022Quote: ... the cells were then incubated for 30 min with anti-active caspase 3 in BD Perm/Wash (BD cat. 554723). Next ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were recovered in MEF media for 48 hours before 3 × 104 - 1.6 × 105 GFP+ cells per sample were sorted with the FACS AriaII (BD Biosciences). Genomic DNA was isolated with Quick-gDNA™ MicroPrep (ZymoResearch ...
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bioRxiv - Neuroscience 2020Quote: ... The iPSCs colonies were identified after approximately 3 weeks in this culture system and subsequently transferred to Matrigel (BD Biosciences) coated plates ...
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bioRxiv - Microbiology 2021Quote: ... Samples were run on a 3 laser Cytek Aurora spectral flow cytometer or a FACSAria II spectral flow cytometer (BD). FCS files were analyzed using Flowjo v10.6.2 software (BD) ...
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bioRxiv - Microbiology 2020Quote: ... and 3×106 cells per tube were stained as follows: cells were incubated in 0.5 μg Fc block (BD Biosciences) for 15 min at 4°C in staining buffer (Ca2+ - and Mg2+ - free PBS containing 3% heat-inactivated fetal bovine serum ...
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bioRxiv - Genomics 2022Quote: Approximately 3 ml of whole blood were collected into heparin-coated Vacutainer® blood collection tubes (BD Diagnostics, Oxford, UK) from all ten non-vaccinated control animals at -1 wk pre-infection ...
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bioRxiv - Genomics 2022Quote: ... Nuclei were finally washed twice in TBS + 3% BSA and resuspended in PI/RNase staining buffer (BD Pharmingen, ref. 550825). All centrifugation steps were performed at 500 g for 1 min at 4°C ...