Labshake search
Citations for Becton, Dickinson and Company :
1601 - 1650 of 1654 citations for hsa mir 373 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... A third of each cell sample was stained for 30 mins at 4°C with 1:100-dilutions of APC-conjugated anti-C-Kit/CD117 (BD Biosciences; 550412) and PE-Cy7-conjugated anti-CXCR-4/CD184 (BD Biosciences ...
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bioRxiv - Cancer Biology 2024Quote: ... Myelin-depleted single-cell suspensions obtained from high grade glioma samples were sequentially labeled using the BD Human Sample Multiplexing Kit (Sample-tag) (BD Biosciences, #633781), the sorting antibody CD45-APC (#560973 ...
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bioRxiv - Microbiology 2024Quote: ... The cells were then resuspended in 500 μL of PBS and then stained following the Fixable Viability Stain 450 kit manufacturer instructions (BD Biosciences, 562247). Infected and uninfected macrophages were stained with Fixable Viability Stain 450 ...
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bioRxiv - Immunology 2024Quote: Intracellular staining for BIM was performed with secondary Donkey anti-rabbit APC secondary antibody using BD cytofix/cytoperm kit (BD biosciences 554722) per manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... Samples were washed twice in staining media and stained for intracellular cytokines or for Foxp3 according to manufacturer recommendations with BD Cytofix/Cytoperm Fixation/Permeabilization Kit (BD 554714, AB_2869008). Samples were further stained with conjugated intracellular antibodies (1:100 ...
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bioRxiv - Immunology 2024Quote: ... whole transcriptome analysis (WTA) libraries were indexed and prepared using the BD Rhapsody™ WTA Amplification Kit (BD Biosciences, Cat. No. 633801).
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bioRxiv - Physiology 2019Quote: Liver tissues were weighed and homogenized in assay diluent provided by the TNF-α or IL-6 ELISA kit (BD, Franklin Lakes, NJ). Plasma samples were collected with heparin as anticoagulant ...
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bioRxiv - Molecular Biology 2020Quote: ... WBM cells were blocked with Fc Blocker and stained with biotinylated-Lineage antibody cocktail according to the Mouse Hematopoietic Stem and Progenitor Cell Isolation Kit by BD (Catalog No. 560492). The stained cells were labelled with streptavidin magnetic particles and placed into BD IMagnet (BD ...
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bioRxiv - Immunology 2019Quote: ... Cytokine concentration was quantified in the supernatants by cytometric bead array (CBA) according to the manufacturer’s instructions from the Mouse/Rat Soluble Protein Flex Set System kit (BD Biosciences Pharmingen, Oxford, UK). CBA data was analysed on a MACSQuant analyser with MACSQuantify™ Software and the CBA analysis software package (BD Biosciences).
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bioRxiv - Cell Biology 2019Quote: ... the plasma levels of pro-inflammatory cytokines IL-6 and TNFα were quantified by bead-based flow cytometry assay (CBA Kit; BD Biosciences, Heidelberg, Germany) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... Supernatant was collected and secreted TNFα protein levels were measured using a BD OptEIA Mouse TNFα Enzyme-Linked Immunosorbent Assay (ELISA) kit (BD Biosciences, Mississauga, ON). Triplicates wells were used for each stimulation condition ...
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bioRxiv - Immunology 2020Quote: ... Cell debris and dead cells were excluded from the analysis based on scatter signals and use of Fixable Blue or Green Live/Dead Cell Stain Kit (BD Bioscience, cat # 564406).
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bioRxiv - Immunology 2020Quote: ... 7 days post-vaccination) and measured Cytokines using the BD CBA Mouse Th1/Th2/Th17 Cytokine Kit (BD Bioscience, San Jose, CA, USA). Sera samples were processed as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... 10 min at room temperature) and the supernatants were collected to measure the levels of IL-2 by ELISA using the OptEIA™ kit (BD Biosciences, USA), according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... Cells were resuspended and stained using cell surface antibody markers preconjugated to a fluorophore: KIT (CD117, BD Pharmingen 25-1171-82, RRID: AB_469644), CD45.2 (BD Biosciences 553772 ...
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bioRxiv - Cancer Biology 2022Quote: The apoptotic rate was determined using Hoechst 33342 staining following fluorescence-microscopic evaluation as well as by annexin V– FITC staining kit (BD Pharmingen, CA, USA) following the manufacturer’s instructions.HCT-116、HCT-116 Bax−/− cells (1×105 cells/well ...
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bioRxiv - Immunology 2024Quote: ... the cells were fixed and permeabilized using the BD Cytofix/Cytoperm™ kit as per the manufacturer’s instructions (BD Biosciences, San Jose, CA). Stained cells were washed twice and data were acquired on a BD LSR Fortessa X-20 (BD Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by washing with PBS and fixation/permeabilization using the BD Cytofix/Cytoperm kit according to the manufacturer’s protocol (BD Biosciences, San Jose, California). Cells were then pelleted and resuspended in the Click-iT OP-puro reaction cocktail with AlexaFluor 488 picolyl azide (Thermo Fisher ...
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bioRxiv - Cancer Biology 2023Quote: ... Intracellular staining was performed after fixation and permeabilisation of the cells using FOXP3 Fixation/Permeabilisation Solution kit (BD Biosciences, Frankin Lakes, NJ, USA) using manufacturer’s instruction ...
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bioRxiv - Immunology 2023Quote: ... 250,000 splenocytes were plated per well to perform an IFN-gamma ELISPOT per protocol using a Mouse IFN-gamma ELISPOT Kit (BD Biosciences; cat. #551083). Each sample was plated in triplicate with peptide or without peptide as a negative control ...
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bioRxiv - Immunology 2023Quote: ... Analysis of intracellular antigens was done by using the same fixation and permeabilization as was described under “Metabolic labeling of nascent proteins” or using BD Cytofix/Cytoperm fixation/permeabilization kit (BD Biosciences, cat#554714). Maximum of 3 x 106 cells per reaction ...
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bioRxiv - Pathology 2023Quote: ... The culture medium was sampled by adding 1-3 drops to microscope slides followed by Gram stain (BD BBLTM Gram Stain Kits; B12539) and imaging.
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bioRxiv - Cancer Biology 2023Quote: Epithelial cell suspensions were stained for viability using LIVE/DEAD™ Fixable Blue Dead Cell Stain Kit (Thermo #L23105) and BUV737 Rat Anti-Mouse CD45 Clone 30-F11 (BD Biosciences # 568344) and analyzed using a 5-laser Cytek Aurora.
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bioRxiv - Cell Biology 2021Quote: ... for 10 min at 37°C and dead cells were labeled using the LIVE/DEAD Fixable Aqua (405 nm) Dead Cell Stain Kit (BD Biosciences, San Jose, CA). EdU detection ...
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bioRxiv - Cancer Biology 2022Quote: ... The co-cultures were incubated for 6 hours at 37°C and 5% CO2 prior to cell staining using the Cytofix/Cytoperm Fixation Kit (BD Biosciences, Cat# 554715/555028).
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bioRxiv - Neuroscience 2021Quote: ... Stimulated cells were stained with fluorochrome-conjugated anti-MCP-1 and anti-IL-6 Abs using the Cytofix/Cytoperm kit (BD Biosciences, San Jose, CA) to determine MCP-1 and IL-6 production ...
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bioRxiv - Physiology 2021Quote: ... islets were dispersed and dead cells were labeled using the LIVE/DEAD Fixable Aqua (405 nm) Dead Cell Stain Kit (BD Biosciences, San Jose, CA). EdU detection ...
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bioRxiv - Immunology 2021Quote: ... T2 and its derived cell lines were fixed and permeabilized using the Cytofix/Cytoperm kit (BD Biosciences, Becton, Dickinson and Company, Franklin Lakes, NJ). Washed cells were then resuspended in 1x PermWash buffer (BD Biosciences ...
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bioRxiv - Cancer Biology 2022Quote: ... IL-2 and IFNγ cytokine concentrations were analyzed by sandwich ELISA according to manufacturer’s kit protocol (BD Opteia human IL-2 and human IFNγ). In vivo cerebellum lysates were analyzed for cytokines similarly ...
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bioRxiv - Immunology 2021Quote: ... followed by a cell viability stain (either Live/Dead Fixable Aqua Dead Cell Stain Kit, 405 nm excitation, Invitrogen ref. no. L34966; or Fixable Viability Stain 700; BD Horizon Cat. No. 564997). For tumor cell analysis ...
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bioRxiv - Immunology 2021Quote: ... PM-WCPV-S and PM-CVHSA-S respectively) in the presence of Brefeldin A (0.65 µL/mL, GolgiPlugTM, BD Cytofix/Cytoperm Kit, Cat. 555028), co-stimulatory antibodies anti-CD28 (BD Biosciences Cat ...
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bioRxiv - Immunology 2019Quote: ... IL-10 and IL-17α were determined in plasma samples through the Mouse Th1/Th2/Th17 Cytometric Bead Array kit (BD Biosciences, San Jose, CA). Bead fluorescence was measured using the BD Biosciences LSRII flow cytometer and analyzed with the accompanying software ...
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bioRxiv - Immunology 2020Quote: We labeled each group of NALT cells separately with the Sample-Tags from the BD Mouse Immune Single-Cell Multiplexing Kit (BD Biosciences, San Jose, CA), described in “Single Cell Labeling with the BD Single-Cell Multiplexing Kits” protocol ...
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bioRxiv - Biochemistry 2020Quote: ... and TNF in the supernatants was determined by using Cytometric Bead Array (CBA) - Human Inflammatory Cytokines Kit (551811, BD Biosciences, San Jose, CA, USA), that allows the determination of the indicated human cytokines simultaneously ...
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bioRxiv - Immunology 2021Quote: ... T cells were stained for surface antigens prior to fixation and subsequently processed for intracellular cytokine staining following BD Cytofix/Cytoperm™ kit (BD Biosciences cat 554714) instructions.
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bioRxiv - Neuroscience 2021Quote: ... 20 μl of each sample was analysed in duplicates using the BD™ CBA Human Soluble Protein Master Kit (#556264; BD Biosciences, CA, USA) alongside the BD™ CBA Human Flex Sets for interleukin 1ß (IL-1ß ...
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bioRxiv - Physiology 2022Quote: ... Then final cDNA library was generated from double strand full length cDNA by random priming amplification with BD Rhapsody cDNA Kit (BD Biosciences, Cat. No. 633773) and BD Rhapsody Targeted mRNA & AbSeq Amplification Kit (BD Biosciences ...
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bioRxiv - Cell Biology 2023Quote: ... density centrifugation or 1X RBC Lysis Buffer (eBioscience) and stained with a combination of fluorochrome-conjugated antibodies: c-Kit (BD Biosciences, BioLegend clone 2B8), Sca-1 (BioLegend clone D7) ...
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bioRxiv - Immunology 2023Quote: ... Following surface staining cells were fixed and permeabilized using a BD Cytofix/ Cytoperm Fixation/ Permeabilization Solution kit (Cat# 554714 BD Biosciences, Franklin Lakes NJ) followed by intracellular staining with anti-IFNγ ...
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bioRxiv - Genetics 2023Quote: ... were isolated by 1X Red Blood Cell lysis Buffer (eBioscience) and stained with a combination of fluorochrome-conjugated antibodies: c-Kit (BD Biosciences, BioLegend clone 2B8), Sca-1 (BioLegend clone D7) ...
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bioRxiv - Genomics 2024Quote: ... the CD34+ cells were carefully pipetted out and their viability was measured using Annexin V apoptosis assay kit (BD Pharmingen, San Diego, CA, USA) as per the manufacturer’s protocol and counter stained with DAPI.
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bioRxiv - Neuroscience 2024Quote: ... PCR amplification of the fragments and their subsequent ligation was performed using the In-Fusion cloning kit and online tools (BD Clontech, Takara Bio, USA), or they were synthesized by GenScript (Piscataway ...
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bioRxiv - Biochemistry 2022Quote: ... cells were fixed with 1% (w/v) paraformaldehyde and 70% (v/v) ethanol on ice and stained with an APO-BrdU TUNEL assay kit (BD Biosciences, San Jose, CA, USA) according to the manufacturer’s protocol and with 1 μg/mL DAPI for 10 min ...
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bioRxiv - Immunology 2019Quote: ... mice were sacrificed and the cells from spleen and peripheral lymph nodes were stained for surface markers and then for BrdU according to the manufacturer’s protocol (BD Pharmingen, APC BrdU flow kit 552598).
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bioRxiv - Immunology 2021Quote: ... or PMA + ionomycin in the presence of brefeldin A for 4 h and stained using the Cytofix/Cytoperm Kit (BD Bioscience, Franklin Lakes, NJ, USA). Foxp3-positive cells and apoptotic cells were detected using the BD Foxp3 Staining Kit (BD Bioscience ...
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bioRxiv - Cancer Biology 2020Quote: ... The viability of the cells was measured post 48 hours of drug treatment using annexin-V-7AAD viability kit (BD Pharmingen, San Diego, CA, USA) as per the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2019Quote: ... and cells in the S phase using incubation with bromodeoxyuridine (BrdU) for 1 h and labeling with an anti-BrdU antibody (APC BrdU flow kit, BD Biosciences, San Jose, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Splenocytes taken from allo-transplanted recipients were stimulated with anti-CD3/anti-CD28 (2.5ug/ml) for 6 hours in the presence of Golgiplug (BD Cytofix/Cytoperm Plus kit cat#555028) (1:1000) ...
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bioRxiv - Microbiology 2022Quote: ... PM-WCPV-S and PM-CVHSA-S respectively) in the presence of Brefeldin A (0.65 µL/mL, GolgiPlug™, BD Cytofix/Cytoperm Kit, Cat. 555028), co-stimulatory antibodies anti-CD28 (BD Biosciences Cat ...
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bioRxiv - Microbiology 2022Quote: ... IL-6 and IL-10 levels in cell culture supernatants were determined using the OptEIA™ mouse IL-10 ELISA kit (BD Biosciences, San Jose, CA) according to the manufacturer’s protocol ...