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Citations for Becton, Dickinson and Company :
1601 - 1650 of 1873 citations for Recombinant Human Complement Factor H His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... Interferon gamma production was assessed by intracellular staining using mouse anti-human IFN-g FITC (BD Bioscience, clone 25723.11) and BD Cytofix/Cytoperm (BD Bioscience ...
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bioRxiv - Immunology 2022Quote: ... Cells were seeded (30,000-50,000/well) into wells pre-coated with anti-human CD3 (2 μg/ml, clone UCHT1, BD Biosciences) along with soluble anti-human CD28 (1 μg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... Surface or total MPL proteins were stained with anti- human MPL-PE antibody (BD Pharmingen 562159; 1:50 dilution).
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bioRxiv - Cancer Biology 2023Quote: ... and stained with the following antibodies: anti-human CD45-APC (clone HI30, #555485, BD Bioscience, Franklin Lakes, NJ, USA) (hCD45 ...
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bioRxiv - Biochemistry 2023Quote: ... 50 μL of 1:250 mouse anti-human CD15 clone HI98 [anti-Lewis x] (BD Biosciences, catalog no. 555400BD) or mouse-anti-human blood group Lewis a clone 7LE (Abcam ...
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bioRxiv - Immunology 2023Quote: ... they were incubated for 10 min at room temperature with a combined Fc block solution (2μL Human Fc block [BD Biosciences ...
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bioRxiv - Molecular Biology 2023Quote: ... GPIIIa, Luc.H11-FITC, #M031-1 or Integrin ⍺ 5/CD49e, Tap.A12-FITC and anti-human CD61-PE, #555754, BD Biosciences) and incubated for 15 min at RT in the dark ...
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bioRxiv - Biophysics 2023Quote: Monoclonal mouse anti-human CD44 (Clone G44-26) and monoclonal mouse anti-CD209 (Clone DCN46) were obtained from BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... 100,000 PBMC per well were seeded in complete media (Advanced RPMI with 10% human serum and 1% L-Glutamine) supplemented with 1µg/mL of anti-CD28 antibody (Clone #37.51, BD Biosciences). The L598 compound was added to the cultures every two days at 2 ...
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bioRxiv - Cell Biology 2023Quote: ... mouse monoclonal antibody against human CD49e/Integrin α5 Chain (1:1000 for western blotting; BD Pharmingen; catalogue number 555614); mouse monoclonal antibody against human Integrin alpha-5 (1:100 for immunofluorescence ...
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bioRxiv - Cell Biology 2024Quote: ... the levels of secreted cytokines were quantified using the BD OptEIA™ Human IL-6 ELISA kit (BD Biosciences). Cells were trypsinized and counted for normalization of cytokine levels per cell.
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bioRxiv - Biophysics 2024Quote: ... to a final concentration of 0.4 mg ml-1 for DHFR or 0.1 mg ml-1 for human fibronectin (BD Biosciences), with a total volume of 100 µl in a 96-well plate ...
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bioRxiv - Molecular Biology 2021Quote: ... It was retrieved from frozen glycerol stocks and cultivated for 24 h at 28 °C on tryptic soy agar (TSA, Becton, Dickinson and Company, USA), followed by culturing in 100 mL of tryptic soy broth (TSB ...
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bioRxiv - Immunology 2021Quote: ... Assay plates were incubated 24-48 h at 37⍰°C in a 5% CO2 incubator and developed using BD ELISpot AEC substrate set (BD Biosciences, San Diego, CA). Spots were counted and analyzed using an ELISpot reader and ImmunoSpot®software v6 (Cellular Technology ...
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bioRxiv - Physiology 2021Quote: ... A 3% BSA-0.2% Triton®X-100 solution was used for permeabilization and blocking followed by a 1 h RT incubation with PBS–1% BSA containing mouse monoclonal DRP1 antibody (611113, BD Transduction Laboratories, 1:50) followed by a 1 h RT incubation with PBS–1%BSA containing rabbit polyclonal RyR2 (PA5-36121 ...
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bioRxiv - Immunology 2021Quote: ... The stimulation was performed by incubation for 6 h at 37°C and 5% CO2 in the presence of Protein Transport Inhibitor Cocktail (brefeldin A) (BD Biosciences, San Jose, CA) at a final concentration of 1:1000 ...
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bioRxiv - Neuroscience 2023Quote: Blood extractions were performed in the morning (8:00-10:00 a.m.) after 8-12 h of fasting and extracted into 10mL BD vacutainer® tubes with K2-EDTA anticoagulant (BD, Franklin Lakes, NJ, USA). Blood samples were immediately processed to obtain plasma and PBMCs based on our previous studies (Orio et al. ...
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bioRxiv - Immunology 2021Quote: ... or PMA + ionomycin in the presence of brefeldin A for 4 h and stained using the Cytofix/Cytoperm Kit (BD Bioscience, Franklin Lakes, NJ, USA). Foxp3-positive cells and apoptotic cells were detected using the BD Foxp3 Staining Kit (BD Bioscience ...
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bioRxiv - Cancer Biology 2019Quote: ... and cells in the S phase using incubation with bromodeoxyuridine (BrdU) for 1 h and labeling with an anti-BrdU antibody (APC BrdU flow kit, BD Biosciences, San Jose, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... All strains were reconstituted and grown anaerobically (6% H, 20% CO2, 74% N2) at 37°C in liquid Brain Heart Infusion medium (BHI; BD Bacto Ref:237500 Lot:1194923) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Cancer Biology 2021Quote: ... Antibodies used are as follows and were used according to manufacturer’s protocol: PE-Cy7 Mouse Anti-Human CD3 (Cat 563423; BD Pharmingen), PE Mouse Anti-Human CD4 (Cat555347 ...
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bioRxiv - Systems Biology 2020Quote: ... Cells were stained at 4°C for 15 minutes with an APC-anti-Human-CD62L (clone DREG-56, BD Pharmingen), a BV650-anti-Human-CD11b (BioLegend ...
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bioRxiv - Immunology 2020Quote: ... 2mM EDTA and 0.05% NaN3): PerCP-Cy™5.5 mouse anti-human CD19 (1:40 dilution, Clone HIB19, BD Pharmingen) (for PBMC samples only) ...
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bioRxiv - Immunology 2022Quote: ... the resulting bulk transduced population positive for Human ACE2 expression stained by FITC-anti-ACE2 Antibody (Sinobiological) was sorted by FACS Aria SORP (BD) and maintained in the culture medium in the presence of 2 μg/ml of puromycin.
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bioRxiv - Bioengineering 2019Quote: ... the monocytes were incubated for 10 min at room temperature with human BD Fc-block (2.5 µg per 1×106 cells in 100 µL, Cat. no. 564220, BD biosciences). The cells were further incubated with the following antibody cocktail for 30 min at 4°C ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were then washed twice 5 min in TBST and incubated with the primary antibody over night at 4°C (human and mouse a-syn: 610786 BD Biosciences ...
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bioRxiv - Systems Biology 2019Quote: Total T cells were isolated from peripheral blood mononuclear cells from 3 human donors via negative selection (Stem Cell Technologie cat. no. 19051) and plated in anti-CD3 (BD Pharmingen clone UCHT1 ...
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bioRxiv - Immunology 2021Quote: ... 1 mM EDTA) with the following anti-human antibodies (all at 1:200 dilution): anti-CD20-PECy7 (BD Biosciences, 335793), anti-CD3-APC-eFluro 780 (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... Phenotyping BCAMhi and BCAMlo organoids: Single cells were incubated with anti-human BCAM BV421 (clone B64; 1:50; BD Biosciences), EGFR BV605 ...
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bioRxiv - Cell Biology 2021Quote: Human THP-1 cells (NCTC #88081201) were maintained or passaged in 25 or 75 cm2 tissue culture flasks (BD Biosciences) using RPMI-1640 (Lonza ...
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bioRxiv - Bioengineering 2020Quote: ... primary human T cells were heated in the thermal cycler prior to assaying with an apoptosis detection kit (BD # 556547) or CellTrace Violet (Fisher # C34571) ...
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bioRxiv - Bioengineering 2021Quote: ... live/dead stain in PBS for 15 min on ice before washing and re-suspending in 2x human/mouse Fc block in stain buffer (BD) followed by surface-staining with monomeric GFP and anti-human CD45 AF700 (Biolegend clone HI30) ...
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bioRxiv - Genomics 2020Quote: Primary human bronchial epithelial basal cells (Passage 1 or 2) were expanded on flasks coated with collagen I (BD Biocoat) in bronchial epithelial growth medium (BEGM) ...
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bioRxiv - Genomics 2020Quote: ... Primary antibodies (anti-Cas9 7A9-3A3, Cell Signaling Technology #14697S, anti-γ-tubulin Sigma#T5326, anti-Human Retinoblastoma protein BD Pharmigen #554136 ...
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bioRxiv - Immunology 2022Quote: ... Pellets were resuspended in 50 µL cold PBS 0.25% BSA with PE-conjugated mouse anti-human IgG (1:100, BD Biosciences), or PE-conjugated goat-anti human IgA (1:800) ...
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bioRxiv - Immunology 2022Quote: ... B cells were also stained with following anti-human antibodies (all at 1:200 dilution): anti-IgD-BV650 (BD, 740594), anti-CD27-BV786 (BD biosciences ...
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bioRxiv - Immunology 2022Quote: ... 1 mM EDTA) with the following anti-human antibodies (all at 1:200 dilution): anti-CD20-PECy7 (BD Biosciences, 335793), anti-CD3-APC-eFluro 780 (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... Samples were stained with fluorochrome-labeled monoclonal antibodies against mouse and human cell-surface antigens and analyzed on an LSRII flow cytometer (BDBiosciences). Data were analyzed using FloJo software.
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bioRxiv - Immunology 2020Quote: ... APC mouse anti-human CD184 (clone 12G5; #555976) and PE rat anti-human CD184 (clone 1D9; #551510) and the appropriate isotype controls were obtained from BD Pharmingen ™ ...
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bioRxiv - Microbiology 2020Quote: ... Samples were taken from the basolateral medium over time and the concentration of chemokines and cytokines was determined using the LEGENDplex Human Anti-Virus Response Panel (13-plex) and the LEGENDplex Human Proinflammatory Chemokine Panel (13-plex) on a FACSCanto II flow cytometer (BD). Samples were run in duplicate and measured at two different dilutions.
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bioRxiv - Cell Biology 2021Quote: ... platelets in ACD-A anticoagulated whole blood were stained with FITC-labelled anti-human CD61 antibody (Clone: RUU-PL7F12, Cat No: 340715 BD Pharmingen ...
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bioRxiv - Cell Biology 2020Quote: ... intranuclear staining was performed at 4 °C overnight in the dark using the FITC anti–human Ki-67 antibody (BD) diluted in Perm/Wash ...
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bioRxiv - Microbiology 2020Quote: ... human ES cell line WA09 was obtained from Wicell International Stem Cell Bank and cultured on Matrigel-coated (BD Biosciences) 6-well plates in mTeSR1 media (Stem Cell Technologies ...
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bioRxiv - Immunology 2021Quote: ... cells were fixed and permeabilized following the protocol and were further incubated with PE-anti-human IFN-γ (4S.B3) (BD) for another 30 min at 4°C followed by flow cytometry ...
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bioRxiv - Immunology 2022Quote: IL13Rα2-CAR T cell variants were stained with fluorochrome-conjugated monoclonal antibodies (mAbs) to human IL13 (BD Biosciences Cat#340508) and CD19 (BD Biosciences Cat#557835) ...
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bioRxiv - Immunology 2022Quote: ... B cells were also stained with following anti-human antibodies (all at 1:200 dilution): anti-IgD-BV650 (BD, 740594), anti-CD27-BV786 (BD biosciences ...
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bioRxiv - Biochemistry 2019Quote: ... The preparation refered here as HLM-1 was prepared by differential centrifugation from the sample of human liver S9 pool (lot number 3212595) obtained from BD Gentest ...
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bioRxiv - Genetics 2020Quote: ... Cells were washed and resuspended in PBS/0.2% BSA for incubation with BUV661 anti-human CD3 monoclonal antibody (UCHT1 clone, BD Biosciences) at 4°C for 30 min in the dark ...
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bioRxiv - Molecular Biology 2019Quote: ... that were either uncoated (unstimulated cells) or coated with anti-human CD3 (1µg/ml, clone UCHT1, no azide/low endotoxin, BD Bioscience) and anti-CD28 (10µg/ml ...
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bioRxiv - Biochemistry 2020Quote: ... were characterized as hADSCs by the expression of CDs by flow cytometry: CD34 (FITC Mouse Anti-human CD34 BD Pharmingen), CD45 (Human CD45 FITC Conjugate ...