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Citations for Becton, Dickinson and Company :
1601 - 1650 of 10000+ citations for Cell Isolation Optimizing System since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Cell suspensions were filtered through a 40 μm filter and analyzed using a FACS Aria cell sorter (BD Biosciences). Color barcodes from marrow samples were quantified using previously published pipelines and adapted to a Python-based interface as previously described (Jon ...
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bioRxiv - Cancer Biology 2024Quote: ... The stained cell suspension was enriched for live CD45+ immune cells using a BD AriaIII flow sorter (BD Biosciences). Sorted live CD45+ cells from each patient were counted and pooled at equal ratios to achieve a total of approximately 20,000 cells in 620μL of ice-cold BD Sample Buffer (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... live cells were stained with Calcein AM (Nacalai) and 20,564 cells were loaded on an BD Rhapsody cartridge (BD) and processed using BD Rhapsody Cartridge Reagent Kit (BD ...
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bioRxiv - Developmental Biology 2020Quote: ... Cells were finally washed with cold PBS and prepared for analysis by flow cytometry using the BD LSR II Flow Cytometer System (BD Biosciences).
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bioRxiv - Microbiology 2021Quote: ... nasopharyngeal (NP) swabs were collected N using the BD Universal Viral Transport Media system (Becton, Dickinson and Company, Franklin Lakes, NJ) from symptomatic patients ...
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bioRxiv - Cancer Biology 2020Quote: ... 100 uL of the Matrigel and coating buffer mix was added to the upper chamber of a 24 well invasion chamber system (BD Bioscience). 1×10^6 cells were plated in the upper chamber ...
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bioRxiv - Microbiology 2021Quote: ... with all test tubes placed into a stainless steel vacuum chamber (BVV, USA) containing a GasPak EZ Anaerobe Container System Sachet (BD, USA) and an anaerobic indicator strip (BD) ...
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bioRxiv - Immunology 2022Quote: Tregs from the lymph nodes and spleen of Foxp3-GFP mice or PD-L1-/- mice were isolated using a FACS Aria II system (BD, USA). Briefly ...
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bioRxiv - Cell Biology 2022Quote: ... and plated on the upper chamber of the 24-well format HTS FluoBlokTM Multiwell Insert System (3 μm pore size; BD Falcon) in RPMI without phenol red (Life Technologies ...
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bioRxiv - Immunology 2020Quote: ... 0.5-1×106 events were collected in each case on a BD LSR Fortessa X-50 flow cytometer (BD Immunocytometry Systems). Electronic compensation was performed with antibody capture beads (BD Biosciences) ...
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bioRxiv - Microbiology 2021Quote: ... Anaerobes were enumerated in a parallel setup following incubation under anaerobic condition using GasPak with EZ Anaerobe Container System (BD, USA). Plates from two dilutions of each sample were counted and the average was reported as colony forming units (cfu ...
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bioRxiv - Genomics 2020Quote: ... ODInCas9 system was left to turn off for 7 days post transfection before assessment using FACS instrument BD LSRFortessa (BD Bioscience) and confocal microscopy instrument Cell Voyager 7000S (CV7000S ...
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bioRxiv - Neuroscience 2019Quote: ... IL-6 and MIP-2 were measured using paired antibodies and recombinant cytokines according to the manufacturer’s recommendations (BD PharMingen Systems) (11).
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bioRxiv - Immunology 2019Quote: ... and IL-1β) were determined using the high-sensitivity cytometric bead array (CBA) flex system (BD Biosciences, San Jose, CA, USA) that allows multiplex quantification in a single sample ...
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bioRxiv - Genetics 2019Quote: ... Staining was carried out using an automated immunostainer (BenchMark XT, Ventana medical systems) as per manufacturers instructions with anti-p62 antibody (BD Biosciences). For western blot the biopsy samples were isolated from the patient and homogenized in a lysis buffer containing 20mM Tris-HCl (pH 7.8) ...
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bioRxiv - Immunology 2019Quote: ... Activation assays were performed by culturing cells with recombinant human IL-2 (10ng/ml, RND Systems) in the presence of coated anti-CD3 and anti-CD28 (2mg/ml, BD Biosciences) in the culture medium ...
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bioRxiv - Molecular Biology 2020Quote: ... Nasopharyngeal (NP) swab specimens were collected using the BD Universal Viral Transport Media system (Becton, Dickinson and Company, Franklin Lakes, NJ) from symptomatic patients.
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bioRxiv - Neuroscience 2021Quote: ... cells in fresh medium were stained with LysoTracker Red for 1 h followed by extensive washing and then transferred into tubes for quantification of lysosome fluorescence using a FACSCanto flow cytometry system (BD Bioscience). Data were analyzed by FlowJo software (Tree Star) ...
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bioRxiv - Cancer Biology 2022Quote: ... incubated with FITC-conjugated anti-BrdU antibody and then analyzed by flow cytometry with a BD FACSCanto II system (BD Biosciences). Data are shown as mean ± standard deviation and are representative of three or four independent experiments ...
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bioRxiv - Immunology 2022Quote: ... Data acquisition and analysis: 0.5-1×106 events were collected on a BD LSR Fortessa X-50 flow cytometer (BD Immunocytometry Systems). Electronic compensation was performed with antibody capture beads (BD Biosciences) ...
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bioRxiv - Microbiology 2024Quote: ... Colonies isolated from growth on Columbia sheep blood agar plates were used to inoculate mNYC III media and cultures were incubated for 48 hours at 37°C under anaerobic conditions (GasPak™ EZ Anaerobe Container System Sachets, BD).
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bioRxiv - Microbiology 2024Quote: ... and NR021 were cultured on Columbia sheep blood agar plates for 48 hours at 37°C under anaerobic conditions (GasPak™ EZ Anaerobe Gas Generating Pouch System with Indicator, BD) from isolates stored at -80°C ...
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bioRxiv - Microbiology 2024Quote: ... which were anesthetized intramuscularly with 10 mg ketamine per kg body weight from the femoral vein employing the Vacutainer system (BD Biosciences). For virus challenges ...
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bioRxiv - Neuroscience 2022Quote: ... were quantitatively determined in spinal cord homogenates by flow cytometry analysis using the mouse Cytometric Bead Array (CBA) Th1/Th2/Th17 kit and specific mouse Flex set systems (BD biosciences), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... P33681) without the leader sequence was PCR-amplified and cloned into the pAcGP67 of the BaculoGold Baculovirus Expression System (BD Biosciences). This vector was modified so that all proteins featured a C-terminal H12-tag ...
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bioRxiv - Microbiology 2022Quote: ... of anaerobic incubation at 37°C in a GasPak jar with an EZ Anaerobe Container System Sachets with Indicator (BD BBL), multiple colonies from each plate were inoculated into two (three ...
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bioRxiv - Cell Biology 2023Quote: ... Media was removed and trypsinization as routine subsequently resuspended in the 500 μL of PBS followed by flow cytometry using FACScan and FACSCalibur systems (BD Biosciences). Data was analyzed with FlowJo™ v10 Software (BD Biosciences).
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bioRxiv - Cancer Biology 2023Quote: ... were added before samples were acquired on an LSR Fortessa equipped with an HTS system and BD FACSDiva software (BD Biosciences).
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bioRxiv - Microbiology 2023Quote: ... and Sharpe (MRS) plates supplemented with 1.5% agar at 37°C in anaerobic conditions (BD GasPak™ EZ Anaerobe Container System) overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell suspensions were incubated in the dark at room temperature for 15min before being analysed by flow cytometry on the LSRFortessa system (BD Biosciences), alongside positive and negative controls ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were then transferred to 5ml BD Falcon tubes and examined with a BD FACSCanto SORP Flow Cytometry System (BD Biosciences). The 70% ethanol fixed Human iPSC sample was a kind gift from Prof ...
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bioRxiv - Molecular Biology 2023Quote: ... Anaerobic bacteria colonies were counted after incubating these agar plates for 2 days using the GasPak anaerobic gas generating pouch system (BD Biosciences).
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bioRxiv - Evolutionary Biology 2024Quote: ... Isolates from the CNRMA in France were subcultured on 2% malt extract agar (Oxoid) and potato dextrose agar (BD Diagnostic Systems) for 5 days at 30 °C ...
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bioRxiv - Genetics 2024Quote: ... and ChIP assays was performed by growing the indicated yeast strains in YPD media for 8 hours using the BD GasPak EZ anaerobe gas generating pouch system (BD 260683). Cells were immediately spun down for one minute and flash frozen to maintain the hypoxic state.
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bioRxiv - Microbiology 2024Quote: ... overnight cultures were either grown with aeration (180 rpm, Infor shaker) in case of ETEC or anaerobically (GasPak™ EZ Systems, BD) in case of B ...
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bioRxiv - Immunology 2024Quote: Cell suspensions were acquired using a fixed and stable flow rate for 2.5 min on a four-laser Fortessa X20 flow cytometer (BD Cytometry Systems). Compensation was performed using OneComp eBeads (01-1111-41 ...
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bioRxiv - Immunology 2024Quote: ... ELISA for human IL-6 and IL-10 were performed using ELISA kits (DuoSet [R&D Systems] or BD OptEIA [BD]) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... ELISA for human IL-6 and IL-10 were performed using ELISA kits (DuoSet [R&D Systems] or BD OptEIA [BD]) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... in BHI medium and grown at 37°C either with aeration for 8 h or overnight anaerobically (GasPak™ EZ Systems, BD). The cells were then removed from the supernatant by centrifugation (9,000 x g ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were sorted for mCherry and EGFP fluorescence using a BD FACSAria™ Cell Sorter (BD Biosciences, San Jose, CA), after 48 h of transduction ...
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bioRxiv - Cancer Biology 2021Quote: ... Antibody incubations were performed for 15 minutes on ice before sorting the cells using a FACSAria II cell sorter (BD).
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bioRxiv - Cancer Biology 2021Quote: ... Cellular uptake was detected in the mCherry channel after acquisition of 10,000 single cells per sample using a flow-activated cell sorter (FACS; FACS Aria III, BD Biosciences). Analyses were conducted by FCS Express (De Novo software).
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bioRxiv - Cell Biology 2020Quote: ... cells were re-suspended in growth media and sorted using a FACSAria IIu cell sorter (BD biosciences, San Jose, USA). The machine was previously calibrated with non-transfected cells of each cell type and baseline for fluorescence was set ...
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bioRxiv - Immunology 2021Quote: Splenocytes and MLN leukocytes were sorted for naïve CD4+ cells (live CD4+ CD25- CD44lo CD62L2+) using a BD FACSAria cell sorter (BD Biosciences). 1×106 cells were plated per well of a 48 well plate pre-coated with 2 µg/ml anti-CD3 and anti-CD28 antibodies for 4 hours at 37°C 5% CO2 ...
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bioRxiv - Genetics 2021Quote: ... 250 µl of cells were transferred into 96-well plates and 30,000 cells were measured in a Fortessa X20 Flow cytometer (BD Biosciences), using the HTS plate mode on the DIVA software and a 488 nm excitation laser to capture the SytoxGreen DNA staining.
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bioRxiv - Developmental Biology 2020Quote: ... Labeled cells were washed and resuspended in Cell Staining Buffer before being analyzed on a LSRII flow cytometer (BD Biosciences) and using software packages CellQuest and FlowJo v10.
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bioRxiv - Genomics 2020Quote: ... Cell aggregates were removed by passing suspensions though a 50 μm cell strainer (FALCON) into a polypropylene FACS tube (BD). To avoid Hoechst toxicity ...
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bioRxiv - Microbiology 2022Quote: ... Cell quantities were quantified with flow cytometry using the BD Cell Viability Kit with BD Liquid Counting Beads (BD Biosciences). Cultures were then centrifuged at 3,000 g for 15 minutes and washed with sterile PBS three times before dilution into sterile water to a final concentration of 9x105 cfu/mL.
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bioRxiv - Molecular Biology 2020Quote: ... Cells were sorted using a 100 μm nozzle (at 20PSI) on a BD FACSARIA III cell sorter (BD Biosciences, UK) with cooling of the sample and collection chambers enabled ...
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bioRxiv - Developmental Biology 2022Quote: ... flow cytometry data acquisition and cell sorting were carried out using FACSAria III cell sorter (BD Biosciences, San Jose/CA). Data were analyzed using FlowJo software version X (FlowJo ...