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Citations for Becton, Dickinson and Company :
1601 - 1650 of 1734 citations for 7 NITRO 3 4 DIHYDROISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: Blood was collected from the saphenous vein at baseline (week 0) and at 3 and 6 weeks in microtainer serum separator tubes (BD Biosciences, Mississauga, ON, Canada). Cleared serum samples were obtained following the manufacturer’s protocol and stored at −20°C until used ...
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bioRxiv - Immunology 2022Quote: ... and F15 NOD Nfκb1 heterozygote were suspended in biotin-free RPMI containing 0.1% azide and 3% FCS and surface stained in 96-well plates with the 10–3.6 PE (anti-I-Ag7) (BD PharMingen, Franklin Lakes, NJ, USA), which is class II major histocompatibility complex (MHC ...
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bioRxiv - Cancer Biology 2024Quote: ... The phosphorylation-SMAD2/3 ability of CAR T cells was detected using PE Mouse anti-Smad2/Smad3 antibodies (clone O72-670, BD Biosciences, Franklin Lakes, NJ) following the staining protocols ...
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bioRxiv - Bioengineering 2023Quote: ... so that the microfluidic tubing (Scientific Commodities,Inc., BB31695-PE/3) can connect the device to the sample sources in a syringe (BD 1 mL, NJ, USA).
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bioRxiv - Bioengineering 2023Quote: ... pneumoniae serotype 3 (Sp3) (ATCC; Manassas, VA) was cultured on plates of BBL Trypticase Soy Agar with 5% sheep blood (BD Trypticase Soy Agar II) (BD Biosciences ...
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Angiogenesis in the mature mouse cortex is governed in a region specific and Notch1 dependent mannerbioRxiv - Neuroscience 2024Quote: ... sections were incubated overnight in 0.1M PBS + 0.1% TX-100 solution containing primary antibody for rat anti-CD31 (label endothelial cells; 1:200 dilution, BD Clone MEC 13:3: #553370) or sheep anti-CD93 (label endothelial tip cells ...
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bioRxiv - Immunology 2021Quote: ... Slides were treated for 10 minutes in H2O2 and after blocking in 10% goat serum in 0.1% Tween20 for 30 minutes and incubated overnight at 4°C with specific antibody diluted in PBS 0,1%Tween20: anti-mouse Ly6G (1A8, BD Pharmingen, cat 551459), or anti-mouse Ki67 (D3B5 ...
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bioRxiv - Neuroscience 2020Quote: ... for 1 hr at room temperature and incubated overnight at 4° C with primary antibodies against Syn1 (mouse, 1:1000, BD Biosciences, #610786), pSer129 (rabbit ...
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bioRxiv - Systems Biology 2019Quote: ... Membranes were incubated with primary antibody solutions (using 5% milk or BSA in TBST) overnight at 4°C at the respective dilutions: E-Cadherin (BD, #610181,1:5000), Vimentin (Abcam ...
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SIMON, an automated machine learning system reveals immune signatures of influenza vaccine responsesbioRxiv - Immunology 2019Quote: ... Cells were then incubated for 1h at RT (or left at 4°C overnight) in the Iridium-intercalator solution in fixation and permeabilization buffer (BD Cytofix/Cytoperm™, BD Biosciences). Then cells were washed with 1x permeabilization buffer (BD Perm/Wash™ ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 4 μL of the saturated culture were transferred in 60 uL PBS and the samples were stored at 4 °C for 1-2 days before being assayed by flow cytometry (BD FACSCanto II). Data were analyzed in FlowJo ...
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bioRxiv - Immunology 2021Quote: For TCR stimulation naïve and primed CD8+ T cells were incubated for 20 min at 4°C with biotinylated anti-CD3ε mAb (5 and 1 μg/mL, BD Biosciences #553060). Cells were washed and stimulated for the indicated time by adding streptavidin (20 μg/mL ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were rinsed once with wash buffer 1 and incubated for two days at 4°C with mouse anti-SR antibody (1:1,000; BD Biosciences; cat #612052). Sections were washed with wash buffer 2 (2% BSA ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were collected and fixed in 4% paraformaldehyde prior to acquisition on a BD LSRFortessa flow cytometer (BD Biosciences, San Jose, CA). CFSE signal intensity of 1 × 104 cells was recorded and analyzed to measure cell divisions ...
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bioRxiv - Bioengineering 2021Quote: ... and stained for 1 hour at 4 °C with 2 µl of the following Abs: PE-Cy7 conjugated anti-mouse IFN-γ (BD Biosciences), PE conjugated anti-mouse IL-2 (Invitrogen eBioscience) ...
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bioRxiv - Bioengineering 2020Quote: ... was applied for 30 minutes at 4°C and stained cells were resuspended in FACS buffer before flow analysis on a BD FACSCanto™ II (BD Biosciences). Gating and flow data were processed on FlowJo (FlowJo LLC).
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bioRxiv - Cancer Biology 2020Quote: ... then probed with the following primary antibodies in 5% milk/TBS-T overnight at 4 °C: SREBP-2 (1:250; BD Biosciences, 557037), p44/42 MAPK (ERK1/2 ...
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bioRxiv - Immunology 2021Quote: ... Cells were then fixed with 4% formaldehyde solution and fluorescence was evaluated on a LSRII flow cytometer (BD Bioscience, San Jose, CA).
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bioRxiv - Immunology 2021Quote: ... Cells were then fixed with 4% formaldehyde solution and fluorescence was evaluated on an LSRII flow cytometer (BD Bioscience, San Jose, CA).
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bioRxiv - Molecular Biology 2020Quote: ... Then the syndecan-4 expression on HUVECs was measured by flow cytometry with a BD Biosciences LSR II (BD Biosciences, CA, USA).
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bioRxiv - Cancer Biology 2021Quote: ... and stained for 1 hour at 4 °C with 2 μl of the following Abs: PE-Cy7 conjugated anti-mouse IFN-γ (BD Biosciences), PE conjugated anti-mouse IL-2 (Invitrogen eBioscience) ...
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bioRxiv - Immunology 2022Quote: ... at 4°C for 5 min before surface staining with a cocktail of the following antibodies for 20 min at 4°C: CD3 V450 (Clone 17A2) (BD Cat. 561389), CD4 BV750 (Clone H129.19 ...
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bioRxiv - Microbiology 2019Quote: ... The resulting solutions were diluted (10-1 - 10-4) and 10 µl were seeded in plates containing potato dextrose agar (PDA) (BD-Difco®) and Sabouraud agar (SBA ...
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bioRxiv - Immunology 2019Quote: Cells were harvested by centrifugation at 400 g for 5 minutes at 4°C followed by staining with fixable viability stain (BD Horizon FVS700) for 10 minutes on ice in PBS ...
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bioRxiv - Cancer Biology 2020Quote: Plasma serum was collected from 4-6 drops of peripheral blood obtained from NSGS mice via submandibular bleeding using Microtainer Collection Tubes (BD Microtainer, # 365967). Concentrations of 29 cytokines/chemokines were measured in 2-4 biological replicates using MILLIPLEX Map Human Cytokine/Chemokine Magnetic Bead Panel (HCYTMAG-60K-PX29 ...
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bioRxiv - Neuroscience 2021Quote: ... A 1ml astrocyte cell suspension of 4×104 cells/ml was plated in three chambers of four-chamber glass slides (BD Falcon, USA) coated with poly-D-lysine ...
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bioRxiv - Immunology 2020Quote: ... and cells were incubated for 4 h at 37 °C in the presence of BD GolgiPlug™ protein transport inhibitor (BD Biosciences). Cells were then labeled with anti-CD8a antibody and a dead cell marker (Live/Dead Fixable Aqua Dead Cell Stain Kit) ...
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bioRxiv - Immunology 2020Quote: ... in house production), 10 μg/mL α-IL-4 (cl. 11B11, in house production) and 10 ng/mL IL-12 (BD Pharmingen) and cultured on goat α-hamster IgG (MP Biochemicals ...
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bioRxiv - Immunology 2019Quote: ... Cells were resuspended in PBS/5%FBS then fixed in an equal volume of 1% paraformaldehyde and stored in the dark at 4°C prior to analysis on a BD LSR FortessaTM or LSR2 flow cytometer (BD Biosciences, UK). Data were analysed using FlowJo software (FlowJo LLC ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were fixed at 4 °C for 20 min and washed in permeabilization buffer from the Fix/Perm kit (BD Biosciences, 554714). Antibodies against intracellular targets were diluted in permeabilization buffer and cells were incubated at 4 °C for 30 min followed by another wash prior to assessment with BD LSR Fortessa SORP cytometer ...
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bioRxiv - Immunology 2022Quote: ... The Fc cell receptors were then blocked for 10 min at 4°C with an anti-CD16/CD32 antibody (10 μg/mL: BD Biosciences 553142) and the cells were resuspended in 25 μL of staining buffer ...
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bioRxiv - Synthetic Biology 2023Quote: ... A third of each cell sample was stained for 30 mins at 4°C with 1:100-dilutions of APC-conjugated anti-C-Kit/CD117 (BD Biosciences; 550412) and PE-Cy7-conjugated anti-CXCR-4/CD184 (BD Biosciences ...
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bioRxiv - Cancer Biology 2024Quote: ... and stained with the following conjugated antibodies (1/100 dilution in PBS) for 15 minutes at 4°C: CD44 (PE, BD Biosciences 555479), EpCAM (FITC ...
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bioRxiv - Microbiology 2023Quote: ... The cells were then washed with flow cytometry buffer [PBS + 0.5% Bovine Serum Albumin (BSA) (Fisher BioReagents, BP9706100) + 2 mM EDTA] and incubated at 4°C with Fc Block (BD Biosciences, 553142) for 10 min ...
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bioRxiv - Cell Biology 2022Quote: ... labelled with cell hashing antibodies and isolated by FACS (all steps at 4°C): Cell suspensions were incubated with Fc-block (1:50, BD Pharmingen 553142) for 10min and subsequently incubated with mouse CD45 MicroBeads (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: ... all cells were harvested from the culture plates and the cell Fc receptors were blocked for 10 min at 4°C with 10 μg/mL anti-CD16/CD32 antibodies (BD Biosciences 553142). Then ...
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bioRxiv - Immunology 2023Quote: ... Stimulated cells were washed once in FACS buffer before fixation for 20 min at 4°C using the BD Cytofix/Cytoperm solution and permeabilization using BD Perm/Wash™ buffer (BD Biosciences). Permeabilized cells were incubated for 30 min with antibodies (diluted 1:200 in BD Perm/Wash ...
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bioRxiv - Genetics 2023Quote: ... Cells were collected every day up to day 4 and were stained with FITC-conjugated IgG1 (1:200, A85-1, BD Pharmigen, 553443) and PE-Cyanine5-conjugated B220 (1:200 ...
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bioRxiv - Immunology 2023Quote: ... PE-cy7-conjugated anti-TNF-α (Clone MP6-XT22, 1:800), and APC-conjugated anti-IL-4 (Clone 11B11, 1:100) (BD Biosciences). All stained samples were acquired using an LSR-Fortessa flow cytometer or Symphony A3 (Becton Dickinson ...
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bioRxiv - Immunology 2023Quote: ... 96-well plates were pre-coated with 10 ng/mL anti-CD3 mAb at 4°C overnight (Purified NA/LE mouse anti-human CD3, BD Cat# 557052). Three-fold serial dilutions of either IL-7 or a test compound were added to the cells and incubated for 4 days at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... treatment was performed at 100μg/ml concentration in dark at 4°C for an hour and analyzed by FACS Calibur flow cytometer and processed using Cell Quest software (BD Biosciences, USA).
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bioRxiv - Cancer Biology 2022Quote: ... 25 μL medium containing 1 × 106 luciferase-expressing MCF10AER/vSrc cells (control or 4-OHT transformed, with or without NLS-catalase expression) were mixed with 75 μL Matrigel matrix (BD Bioscience, #356237) containing 10 μM 4-OHT and injected into the inguinal mammary fat pad of the female mice at 6 to 8 weeks of age ...
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bioRxiv - Immunology 2023Quote: Splenocytes-or BM-derived cells were resuspended in sPBS and the Fc receptors were blocked for 10 min at 4°C with 10 μg/mL anti-CD16/CD32 antibodies (BD Biosciences 553142). After blocking ...
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bioRxiv - Genomics 2023Quote: Trypsinized or MACS sorted cells were incubated at 4°C for 20 min with APC-conjugated anti-CD56 antibodies (1:40, BD Pharmingen #555518) or isotype control (1:40 ...
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bioRxiv - Genomics 2023Quote: Whole blood aliquots at a volume of four (4) milliliters were collected from participants using sterile Vacutainer blood collection kits (BD Vacutainer®) and subsequently biobanked at −80°C for DNA extraction ...
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bioRxiv - Bioengineering 2023Quote: ... for 20min at 4°C and stained with intracellular antibodies including anti-mouse IFN-γ-Pe-Cy7 and IL-2-BV605 (BD Bioscience) for 30min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Blood was collected via submandibular bleeds with goldenrod 4 mm lancets collected into sample tubes coated with K2 EDTA (BD Ref# 365974) and 60 μL of blood was lysed with 500 uL of ACK lysis buffer ...
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bioRxiv - Cell Biology 2023Quote: The following antibodies were used in this study: rabbit anti-AP-4 epsilon 1:400 for Western blots (612019; BD Transduction Labs); rabbit anti-ATG9A 1:200 for immunofluorescence and 1:1000 for Western blots (ab108338 ...
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bioRxiv - Cell Biology 2024Quote: ... Cels were fixe with 4% PFA and stained with Rb anti-HA for NDST1 (CST 3724S) and Ms anti-GM130 (BD biosciences 610823) for Golgi followed by secondary antibodies staining with anti-Rb-A550 and anti-Ms-A647 ...
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bioRxiv - Bioengineering 2024Quote: ... A third of each cell sample was stained for 30 mins at 4°C with 1:100-dilutions of APC-conjugated anti-C-Kit/CD117 (BD Biosciences; 550412) and PE-Cy7-conjugated anti-CXCR-4/CD184 (BD Biosciences ...