Labshake search
Citations for Becton, Dickinson and Company :
1551 - 1600 of 9453 citations for Mouse Eukaryotic Translation Initiation Factor 4E Binding Protein 1 EIF4EBP1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: ... 0.5–1 million cells were stained with one of the following primary antibodies: mouse anti-PSGL1 antibody (KPL-1) (BD Pharmingen), mouse anti-CD43 antibody (1G10 ...
-
bioRxiv - Immunology 2020Quote: ... The cells were fixed with 4% neutrally buffered formalin for 5 min and stained with anti-mouse primary antibodies (1:100, anti-mouse LC3B, LAMP-1 and p62 antibodies) followed by a biotinylated anti-Ig secondary antibody (BD Biosciences) and streptavidin-PE ...
-
bioRxiv - Immunology 2021Quote: ... the sera were diluted 1/10 with PBS containing 1% (w/v) BSA and loaded onto anti-mouse IgE coated (BD biosciences) plates anti-OVA-HRP antibodies (AbD Serotec ...
-
bioRxiv - Molecular Biology 2023Quote: ... For BrdU staining we used either a rat anti-BrdU (1:1000, Eurobio clone BU1/75) or mouse anti-BrdU (1:1000, BD 347580) antibodies 2 hours at RT (1:1000 ...
-
bioRxiv - Physiology 2024Quote: ... The 2X stain cocktail included the following antibodies (final dilutions are all 1:300 unless otherwise indicated): rat anti-mouse SiglecF-BV421 (1:400, clone E50-2440, cat# 562681 BD Horizon), rat anti-mouse/human CD11b-Pacific Blue (clone M1/70 ...
-
bioRxiv - Microbiology 2023Quote: ... for 16 hours followed by stimulation with a peptide pool of a broad range of HLA-subtypes and infectious agents (CEFX Ultra SuperStim Pool; JPT) (1 µg/ml) and 1 μg/ml mouse-anti-human CD28 antibody (#556620; BD Biosciences), or IL-2 (20 IU/ml ...
-
bioRxiv - Cancer Biology 2024Quote: ... 3 × 106 VCaP cells were subcutaneously injected into the dorsal flank on both sides of each mouse in a 1:1 mixture of serum free medium and Matrigel (BD Biosciences). Once tumors grew to a palpable size (∼400 mm3) ...
-
bioRxiv - Bioengineering 2024Quote: ... Cells were then washed 2x with 1% FBS in PBS and incubated in 1% FBS in PBS with APC-conjugated mouse anti-human CD68 (BD Biosciences) for 1 hour at room temperature ...
-
bioRxiv - Molecular Biology 2020Quote: ... CD34-FITC according to manufactures recommendations (Mouse Hematopoietic Stem and Progenitor Cell Isolation Kit, BD, Catalog No. 560492). LSKCD34low cells were analyzed by flow cytometer (BD FACS Calibur ...
-
bioRxiv - Immunology 2020Quote: ... TNF-α e IL-12p70 were measured using BD Cytometric Bead Array (CBA) Mouse Inflammation kit (BD Bioscienses) according to manufecturer’s protocol ...
-
bioRxiv - Immunology 2023Quote: ... Interferon (IFN)-γ-positive cells were detected using a mouse IFN-γ ELISPOT Kit (BD, Cat.#551083, U.S.), and interleukin (IL)-4-positive cells were detected using a mouse IL-4 ELISPOT Kit (DAKEWE ...
-
bioRxiv - Neuroscience 2023Quote: ... astrocytes (1×106) were incubated with protein transport inhibitor containing Brefeldin A (555029 Golgiplug, BD Biosciences) and Monensin (554724 Golgistop ...
-
bioRxiv - Cancer Biology 2023Quote: ... 1% penicillin/streptomycin and 10 mM HEPES [pH7.4]) with 25% high protein Matrigel (354248; BD Biosciences). PDX single cell suspensions were obtained by mechanically dissociated (12-141-363 ...
-
bioRxiv - Developmental Biology 2022Quote: ... anti-KIT rat monoclonal antibody (BD Biosciences, 553352, 1:200), anti-DDX4 rabbit polyclonal antibody (abcam ...
-
bioRxiv - Genomics 2019Quote: ... permeabilization and intracellular staining for Ikaros and BCL-6 was performed using the Transcription Factor Buffer Set (BD Pharmingen). Cells were analyzed on a CytoFLEX flow cytometer (Beckman Coulter).
-
bioRxiv - Cell Biology 2021Quote: ... which were triturated and re-plated on tissue culture dishes coated overnight with growth-factor-reduced Matrigel (BD Biosciences).
-
bioRxiv - Developmental Biology 2022Quote: ... generated EBs were collected and embedded in 50μl (around 40 EBs) of growth-factor-reduced Matrigel® (BD Biosciences), plated in 12-well plates and treated for three days with 1 mg/ml Doxycycline (Sigma) ...
-
bioRxiv - Cancer Biology 2022Quote: ... the transwell inserts from the CIM plates were coated with 20 μl of growth-factor-reduced Matrigel (BD Biosciences), at a concentration of 250 μg/ml ...
-
bioRxiv - Cancer Biology 2019Quote: ... Intracellular cytokine and transcription factor staining were performed right before analysis using either the BD Perm/Wash Buffer (BD) or the Foxp3 Transcription Factor Staining Buffer Kit (eBioscience) ...
-
bioRxiv - Developmental Biology 2021Quote: ... from E16.5 C57BL/6 lungs pooled by litter and cultured on a layer of growth factor reduced Matrigel (BD) in serum-free media (DMEM/F12 supplemented with L-glutamine and penicillin-streptomycin ...
-
bioRxiv - Cell Biology 2023Quote: ... the following were also supplemented: 20 ng/mL brain-derived neurotrophic factor (BDNF; R&D systems, catalog #: 248-BD), 20 ng/mL glial cell line-derived neurotrophic factor (GDNF ...
-
bioRxiv - Immunology 2023Quote: ... LPLs were then stained with the following transcription factor antibodies for 30 min at 4°C: RORψt (Q31-378, AF647, BD) and Foxp3 (FJK-16s ...
-
bioRxiv - Molecular Biology 2021Quote: Pan-ubiquitin mouse monoclonal #3939 (Cell Signalling Technology) Mouse/human RIPK1 mouse monoclonal #610458 (BD transduction Laboratories)
-
bioRxiv - Cell Biology 2020Quote: ... Anti-Bip (Mouse, 610979), anti-GM130 (Mouse, 610822) and anti-Tim23 (Mouse, 611223) antibodies were from BD Transduction ...
-
bioRxiv - Immunology 2024Quote: ... the intracellular expression of viral p27 Gag proteins was assessed by flow cytometry with a CytofixCytoperm kit (BD Biosciences). Antibodies used were as follows ...
-
bioRxiv - Cancer Biology 2021Quote: ... cells were resuspended in 200 μL 1x binding buffer with or without 5 μL Annexin V (BD Biosciences, Billerica, MA). After 15 min incubation ...
-
bioRxiv - Plant Biology 2020Quote: ... 10 μL of 10-fold serial dilutions of yeast harbouring an activation domain (AD)-AtCBLs or empty vector pTOOL28 and either DNA-binding domain (BD)-AtCIPK16 or empty vector pTOOL27 were spotted on plates containing DDO (SD/-Leu/-Trp ...
-
bioRxiv - Developmental Biology 2021Quote: ... and another one bearing amino acids 329–542 were cloned into the pOAD and or pOBD2 vectors (Cagney et al., 2000) in-frame either with the activator domain (AD) or the DNA-binding domain (BD) sequence of GAL4 ...
-
bioRxiv - Pathology 2019Quote: ... and V2C84AC86A were cloned into the activation domain (AD)-containing vector and SlSGS3 was cloned into the vector harboring the DNA binding domain (BD). Both constructs were transformed into Saccharomyces cerevisiae strain AH109 ...
-
bioRxiv - Immunology 2019Quote: ... were labeled with a cocktail of non-APC binding antibodies namely the murine T-cell lineage directed anti-Thy-1.2 antibody (BD Pharmingen) and the T-cell subsets recognizing CD4 antibody (eBioscience ...
-
bioRxiv - Biochemistry 2021Quote: ... The expression and binding signals were determined by flow cytometry using BD Accuri™ C6 Flow Cytometer (BD Biosciences, USA). The cell analysis and sorting were done by S3e Cell Sorter (BioRad ...
-
bioRxiv - Cancer Biology 2020Quote: ... coding sequence were inserted into pGADC1 vector harbouring GAL4 activation domain (AD) and pBDUC1 vector harbouring GAL4 DNA binding domain (BD).
-
bioRxiv - Neuroscience 2020Quote: The vector used to insert the bait cDNAs in the yeast two-hybrid (YTH) screens was Gal4 DNA binding domain (Gal4-BD) expression vector pAS2-1 (Clontech ...
-
bioRxiv - Microbiology 2020Quote: ... Gene sequences without predicted introns were inserted into the vectors pGAD24 and pGBKT7 generating translational fusions to the Gal4 activation domain (AD) and DNA-binding domain (BD), respectively.
-
bioRxiv - Cancer Biology 2022Quote: ... Cells were resuspended in 200 uL of binding buffer after discarding the supernatant and analyzed via flow cytometry using a FACSCalibur Flow Cytometer (BD).
-
bioRxiv - Molecular Biology 2020Quote: ... CDS (pENTR/D-TOPO) were sub-cloned into both pGADT7-GW (activation domain, AD) and pGBKT7-GW (binding domain, BD) to generate SWCDS-AD and SWCDS-BD ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cells were resuspended in 200 uL of binding buffer after discarding the supernatant and analyzed via flow cytometry using a FACSCalibur Flow Cytometer (BD).
-
bioRxiv - Cancer Biology 2019Quote: ... and resuspended in Annexin V binding buffer (10 mM HEPES 7.4, 140 mM NaCl, 2.5 mM CaCl2) with Annexin-V FITC (BD Biosciences 556419). Flow cytometry was performed on a Becton Dickinson LSRFortessa or LSRII flow cytometer ...
-
bioRxiv - Microbiology 2020Quote: ... cells were resuspended and 2-43 binding to cell-surface S trimer was quantified on LSRII flow cytometer (BD Biosciences). The mean fluorescence intensity of APC in GFP-positive cells was analyzed using FlowJo and the relative binding of 2-43 to S trimer in the presence of competitors was calculated as the percentage of the mean fluorescence intensity compared to that of the competitor-free controls.
-
bioRxiv - Microbiology 2021Quote: ... from pDONR207 into the yeast two-hybrid vector pPC97-GW for expression in fusion downstream of the GAL4 DNA-binding domain (GAL4-BD). The control siRNA and a pool of TAX1BP1 siRNA (Ambion ...
-
bioRxiv - Genetics 2020Quote: ... Both WT and TG8B RTY were transferred to pACT-GW (GAL4-Activation Domain, AD) and pAS-GW (GAL4-Binding Domain, BD) (Nakagawa et al. ...
-
bioRxiv - Cell Biology 2022Quote: ... was then transferred to a 96-well plate and fALOD4-Neon binding was measured by analyzing 10,000 RBCs for each condition on a Stratedigm S1000 EX Flow Cytometer (BD Biosciences). Fluorescently labeled RBCs were analyzed using FlowJo software version 10 (Ashland ...
-
bioRxiv - Plant Biology 2022Quote: ... while the full-length coding sequence of HlMYB7 was cloned into the prey vector pGADT7-GW [DNA-binding domain (BD)] (Addgene Inc ...
-
bioRxiv - Plant Biology 2022Quote: ... and the PCR products were fused into the activation domain (AD) vector pGADT7 and/or the DNA-binding domain (BD) vector pGBKT7 and verified by sequencing ...
-
bioRxiv - Immunology 2022Quote: ... the amplified PGRP3 cDNA was digested with BamHI and EcoRI and ligated to a pGBKT7 vector containing the GAL4 DNA-binding domain (BD). Successful recombinant pGBKT7-PGRP3 bait construction was verified with restriction digestion (BamHI ...
-
bioRxiv - Cell Biology 2023Quote: ... of BicDR were cloned into pOAD and pOBD2 vectors so that they were in frame with the activator domain (AD) or the DNA binding domain (BD)13,30 ...
-
bioRxiv - Plant Biology 2023Quote: ... Obtained fragment was cloned into pDONR207 via Gateway BP reaction with following LR reactions into pGBKT7-GW (binding domain, BD) or pACT2-GW (activation domain ...
-
bioRxiv - Immunology 2023Quote: ... or showed a complete loss of antibody binding (AF647-/PE+) were isolated using FACS (BD Aria Fusion or Influx BD). Collected cells were pelleted by centrifugation at 3500 x g for 3 min at room temperature ...
-
bioRxiv - Cancer Biology 2023Quote: ... The binding of IGSF3-Fc to the cell surface was detected as the APC fluorescence intensity using FACSVerse (BD Biosciences).
-
bioRxiv - Cell Biology 2023Quote: ... Full-length or truncation (ΔTM) of TbPEX15 with and without mutations were cloned in the pPC97 vector containing GAL4- DNA Binding Domain (BD) as described in Table 1 ...