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Citations for Becton, Dickinson and Company :
1501 - 1550 of 1798 citations for 3 O tert Butyldimethylsilyl 24 ethyl 24 phenylsulfonyl cholest 5 ene 3 ol d7 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... 50 μL of the stain cocktail (1x Brillant Stain Buffer [BD Bioscience] and either 5 μL per sample of Alexa Flour 647 Anti-Total Stat1 [N-Terminus] [BD Biosciences] and 5 μL per sample of PE anti-STAT1 Phospho [Tyr701] [BioLegend] ...
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bioRxiv - Microbiology 2023Quote: ... Experimental starters were made from master glycerol stocks struck on 5% Columbia blood agar plates (Biomerieux Ref# 43041) and grown overnight at 37°C with 5% CO2 prior to outgrowth in Todd-Hewitt (BD) broth supplemented with 50 mM HEPES (Sigma ...
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bioRxiv - Genomics 2023Quote: ... Pools of ciliate cells (5 – 50 cells) were then sorted into a 384-well plate containing 5 μL of autoclaved source water using FACS (BD FACSMelody™ Cell Sorter ...
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bioRxiv - Cancer Biology 2022Quote: ... Single cell suspensions were washed with 1XPBS-4%FBS before incubation for 20 min on ice at 5•107 cells/ml with 500 ng/ml Fc block (2.4G2, BD Pharmingen). Cells were then incubated for 1 hour on ice with AF700 conjugated CD45.2 monoclonal antibody (104 ...
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bioRxiv - Cancer Biology 2024Quote: ... 5×104 alive GAFs or glioma cells were incubated with PE Mouse anti-Human CD105 (2µg/mL, BD Biosciences #560839) and APC Mouse anti-Human CD90 (2µg/mL ...
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bioRxiv - Microbiology 2024Quote: Sequential subcultures of all phenotypic variants were carried out on various agar plates (including Columbia agar + 5% sheep blood, MacConkey agar from BD, and CHROMID® CPS® Elite agar from bioMérieux ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cells (1.5 x 106 cells) first underwent Fc receptor blockade with rat anti-mouse FcψRIII/II receptor (CD16/32; BD Biosciences). After blocking for 5 minutes on ice ...
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bioRxiv - Cell Biology 2023Quote: ... The worms were immobilized with levamisole (final concentration 200 µM) and cut open using with a pair of 30G 5/8” needles (PrecisionGlide, BD) in a scissors motion to extract the tissues ...
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bioRxiv - Immunology 2024Quote: ... Tregs were then sorted on a BD FACS Aria Fusion based on viability dyenegative CD4+ (BV605, clone RM4-5, BD) GFP-FOXP3+ ...
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bioRxiv - Immunology 2024Quote: ... live EGFP+ or EGFPneg Tcf7+/EGFP P14 CD90.1+ TE (Zombie Aquaneg EGFP+ or EGFPneg CD8a+ CD90.1+) from helped or helpless spleens were sorted on a 5-laser FACS Aria II sorter (BD Biosciences). Small aliquots of sorted samples were checked for purity (on average >98%) ...
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bioRxiv - Immunology 2024Quote: ... cells were treated for 5 hours with a protein transport inhibitor containing brefeldin A (BD Biosciences, Franklin Lakes, NJ, USA), washed and fixed for 10 minutes at 4°C with fixation/permeabilization solution (BD Cytofix/Cytoperm kit ...
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bioRxiv - Immunology 2024Quote: ... Mouse peripheral blood was collected via submandibular bleed using Goldenrod 5 mm animal lancets (MEDIPoint) into K2EDTA microtainer tubes (BD). For all specimen types ...
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bioRxiv - Microbiology 2024Quote: ... A single isolated colony was then inoculated in 5 ml of BHI broth (BD, cat. no. 237500, Franklin Lakes, NJ) in a 15 ml conical tube (VWR ...
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bioRxiv - Developmental Biology 2023Quote: ... collected by centrifugation at 300 g for 5 min and passed through a 35-µm cell strainer filter (BD Biosciences). To assess viability ...
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bioRxiv - Bioengineering 2023Quote: ... A tissue suspension was constituted by adding 5 mL of Tumor & Tissue Dissociation Regent (TTDR, BD Bioscience, San Jose, CA), and then incubating the mixture at 37 °C for 30 min with frequent agitation ...
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bioRxiv - Developmental Biology 2024Quote: ... Membranes containing the transferred proteins were rinsed and blocked in 0.1% Tween-20 in Tris-buffered saline (TBST) containing 5% nonfat milk (BD Biosciences) at RT for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... All dissociated cells were blocked with Fc receptors staining buffer (1% anti-mouse CD16/CD32, 2.4 G2, 2% FBS, 5% NRS, and 2% NMS in HBSS; BD Bioscience) and then stained with a standard panel of antibodies ...
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bioRxiv - Genomics 2023Quote: The following antibodies were used for Immunoblot analysis (Extended Data Table 5): Purified Mouse Anti-PTEN (BD Bioscience, Cat# 559600), Phospho-Akt (Ser473 ...
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bioRxiv - Immunology 2023Quote: ... high-binding 96-well plates were coated with 5 μg/ml anti-CD3 (Clone UCHT1, BD Pharmingen™ #555329, USA) in PBS (ThermoFisher Gibco™ #21600044 ...
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bioRxiv - Physiology 2024Quote: ... Cells were then pelleted at 500 x g for 3 min at 4°C and resuspended in 25 μL of 5 mg/mL FcBlock (rat anti-mouse CD16/32, clone 2.4G2, BD Biosciences) diluted in FACS Buffer and incubated on ice for 10-15 min ...
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bioRxiv - Developmental Biology 2024Quote: ... 2.5 % donkey serum in PBST containing 0.2% Triton X-100) and incubated with α-EPS8 antibody produced in mouse (BD Transduction Laboratories™ ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed twice (900 x g for 5 minutes at room temperature) and then 50µL of surface antibody cocktail diluted in Brilliant Violet buffer (BD Biosciences) added and incubated 20 minutes at room temperature in the dark ...
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bioRxiv - Neuroscience 2022Quote: ... resuspended in 500 μL FACS buffer and transferred to 5 mL round-bottom FACS tubes through cell strainer caps (BD). Cells were sorted through a 100 μm nozzle on a FACS Aria II (BD ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were probed for non-glucosylated Rac1 in Tris -buffered saline containing 0.1% Tween (TTBS) with 5% milk and 1:1,000 mouse anti-Rac1 (Clone 102/Rac1 610650; BD Bioscience, USA) and then incubated overnight at RT and 6 h at RT ...
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bioRxiv - Cancer Biology 2022Quote: ... Gates were determined using the isotype controls mouse anti-IgG2b κ (APC-conjugated, Clone 27-35, 1:5, BD Biosciences) and mouse anti-IgG2a κ (PE-Cy7-conjugated ...
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bioRxiv - Bioengineering 2022Quote: ... DOs-laden microspheres were incubated with Williams’ E medium (BasalMedia, L660KJ) plus 5 µM cholyl-lysyl-fluorescein (CLF, BD-451041) for 15 minutes at 37 °C and 5% CO2 ...
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bioRxiv - Immunology 2022Quote: ... HSP+TCR condition – cells cultured with IL-15 and -7 and activated by anti-CD3 (5 μg/ml, BD Biosciences) and CD28 (1 μg/ml ...
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bioRxiv - Microbiology 2022Quote: ... swabs of 0.5 MacFarland suspensions prepared from isolated colonies were inoculated for confluent growth on cation-adjusted Mueller Hinton agar with 5% sheep blood (BBL, Becton-Dickinson, Franklin Lakes, NJ) using discs (BD) containing conventional drug masses ...
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bioRxiv - Immunology 2024Quote: ... stained with 2.5 μM cisplatin for 5 min at 25°C and fixed using transcription factor buffer set (BD Biosciences), followed by intracellular staining for 60 min at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... incubated in a 5% CO2 incubator and then stored in brain heart infusion broth (BHI; BD, Franklin Lakes, NJ, USA) with 20% glycerol at −80°C before being shipped to our lab at NDSU ...
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bioRxiv - Microbiology 2024Quote: ... 10 µL of the bacterial suspension was dropped onto Trypticase soy agar with 5% sheep blood (Becton, Dickinson and Company) and cultured at 27°C for 3 days ...
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bioRxiv - Microbiology 2024Quote: ... The homogenates were diluted with GZM and spread on Trypticase soy agar containing 5% sheep blood (Becton, Dickinson and Company). After incubation at 25°C for 3 days ...
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bioRxiv - Immunology 2024Quote: ... Blood isolates were then pelleted (500xg for 5 min) and subjected to two rounds of red blood cell lysis (1X Pharm-Lyse solution; BD) prior to antibody staining for flow cytometry ...
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bioRxiv - Immunology 2024Quote: ... or 5 x 106 wild type HT1080 (HT1080-WT) cells serving as negative control were resuspended in 50% Matrigel (BD) and 50% PBS and subcutaneously injected into the right upper flank of 7-week-old CD1 nude mice (Charles River Laboratories) ...
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bioRxiv - Immunology 2024Quote: ... Isolated T cells were labeled with 5 μM carboxyfluorescein succinimidyl ester (CFSE)-Violet Proliferation Dye 450 (VPD450, BD Horizon, 562158) for 25 min ...
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bioRxiv - Immunology 2024Quote: ... 20min at 4°C and then blocked with 100µl of PBS/2mM EDTA + FBS 5% + 2,5 ng/ml purified rat anti-mouse CD16/CD32 (BD Biosciences #553141) 20min at 4°C ...
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bioRxiv - Immunology 2024Quote: ... Plates were incubated for 1 hour with secondary antibody and washed with PBST 5 times before adding TMB substrate (BD) to develop the signal The reaction was stopped with 2N H2S04 and ELISA plates were read on a Tecan infinite M1000pro plate reader ...
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bioRxiv - Microbiology 2024Quote: ... The overnight cultures of Pseudomonas aeruginosa ATCC27853 and EXT111762 were adjusted to McFarland Standard 1 and further diluted to 5 × 105 cells mL−1 in cation adjusted Mueller Hinton II broth (BD). A volume of 5 mL was distributed to each cultivation flask and supplemented with either 0.5x ...
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bioRxiv - Immunology 2024Quote: ... according to manufacturer’s instructions to exclude dead cells and then labelled in FACS buffer with the following antibodies: V500 Rat anti-Mouse CD4 (RM4-5, BD), eF450 CD5 Monoclonal Antibody (53-7.3 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... for 5 min at 4 °C and immunostained with CD11b-AlexaFluor 647 (M1/70; 1:1000; Cat#557686, BD Biosciences) and CD45-PE (1:1000 ...
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bioRxiv - Cancer Biology 2024Quote: ... The tissue suspension was filtered through a 5 mL Round Bottom Polystyrene Test Tube with Cell Strainer (BD Flacon, 352235), and the cell suspension obtained by filtration was fixed in 1% formaldehyde/DMEM buffer for 30 min at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 120 µL of a cell suspension in McCoy’s (+10 % FBS) containing 5 x 104 cells/mL were seeded into black 96-well imaging plate (BD Falcon). The plates were incubated for two days (37 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... UK), mouse monoclonal PARP (1:1000, #556494, clone 4C10-5) and mouse monoclonal cytochrome-c (1:1000, #556433) from BD Pharmingen (Heidelberg ...
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bioRxiv - Cancer Biology 2024Quote: ... Splenocytes were spun at 300 × g for 10 minutes and then subjected to Red Blood Cell lysis (RBC) using 5 ml of BD Pharmalyse (BD). After 5 minutes of incubation ...
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bioRxiv - Cell Biology 2024Quote: ... for 1 hour and probed overnight at 4°C with the following primary antibodies diluted in 5% non-fat dry milk/TBS-T: mouse monoclonal anti-p150 clone 1/p150Glued (BD Transduction Laboratories 610473 ...
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bioRxiv - Microbiology 2020Quote: ... washed with cold PBS by centrifugation (5 min, 550g at 4°C) and analysed with a FACS BD AccuriTM C6 (BD Biosciences) instrument ...
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bioRxiv - Cell Biology 2020Quote: ... cells were re-suspended in 150 μl of PBS/5% FBS and analyzed on a BD FACS Canto2 flow cytometer (BD Biosciences) for PE-Cy7 expression ...
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bioRxiv - Cell Biology 2020Quote: ... was prepared the day before the experiment by incubating the wells with 50μl/well of 5 μg/ml of anti-integrin α5 antibodies (BD Biosciences, 555651) in 0.05m Na2CO3 pH 9.6 ...
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bioRxiv - Cell Biology 2020Quote: ... The CSCs were selected as the top 5% cells with the highest GFP intensity (Oct4hi) and then sorted with the FACSAria II cell sorter (BD Biosciences).
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bioRxiv - Immunology 2021Quote: ... 10% FCS and incubated with the indicated pool of peptides (5 μg/ml final concentration of each peptide) and brefeldin A (1 μg/ml; BD Pharmingen) at 37°C for 12-16 hours ...