Labshake search
Citations for Becton, Dickinson and Company :
1451 - 1500 of 1553 citations for hsa mir 483 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: Cells were directly sorted into 1.5 ml tubes containing 100 µl of extraction buffer (XB, Arcturus PicoPure RNA isolation kit) with a FACSAria III SORP (BD Biosciences) flow cytometer equipped with a 90 µm-diameter nozzle ...
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bioRxiv - Cell Biology 2023Quote: ... MPP1-4 from WT and mutant mice were permeabilized and fixed according to the manufacturer’s instructions with the FOXP3 permeabilization kit (Foxp3/Transcription Factor Staining Buffer Set) and then labeled with Ki-67 Ab (clone B56, mouse IgG1; BD Biosciences) and DAPI before flow cytometric analysis ...
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bioRxiv - Immunology 2023Quote: ... Medium was exchanged after 48 h of incubation and after 72 h cells were washed and permeabilized/fixed using Cytofix/Cytoperm kit (BD Biosciences). Cells were stained with VL-4 hybridoma cell line supernatant containing LCMV NP-specific antibodies for 60 min at RT ...
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bioRxiv - Cell Biology 2023Quote: ... 10-μM bromodeoxyuridine (BrdU) solution was added 4 h before the end of the experiment [fluorescein isothiocyanate (FITC) BrdU Flow Kit (BD #559619)] according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... or (ii) PE-Annexin V conjugated antibody (1:33 dilution in TBST) and 7-AAD as per kit instructions (BD Pharmingen). Samples were analysed by flow cytometry within 30 min.
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bioRxiv - Immunology 2023Quote: ... Cytokine concentrations in the supernatants of DC cultures were measured using a BD human inflammatory cytometric bead array kit (BD #551811) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... and either resuspended in PBS and directly analyzed or fixated using the BD Cytofix/Cytoperm fixation and permeabilization kit (BD Bioscience) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... IL-6 and IL-1β cytokines were measured in the cell culture supernatant after 2 h of co-exposure using CBA human Inflammatory cytokine kit (BD Biosciences), while chemokines CXCL8/IL-8 ...
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bioRxiv - Microbiology 2023Quote: ... Afterwards cells were washed twice with PBS and permeabilized 20 minutes with 200µl BD Cytofix/Cytoperm™ Fixation/Permeabilization Solution Kit (BD Biosciences)at RT ...
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bioRxiv - Cancer Biology 2023Quote: Cell cycle analysis was performed by in vitro labeling of cells with the APC BrdU Flow Kit (cat. no. 552598, BD Biosciences). First ...
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bioRxiv - Neuroscience 2023Quote: ... and tumor necrosis factor (TNF) in cell supernatants were measured using the Human Inflammatory Cytokine Cytometric Bead Array Kit (BD Biosciences). Concentrations of chemokines in cell supernatants were measured using the LEGENDplexTM Human Proinflammatory Chemokine Panel 1 (Biolegend) ...
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bioRxiv - Genomics 2023Quote: ... at least 2 × 106 cells were fixed and permeabilized using the Fixation/Permeabilized Solution Kit with BD GolgiStop TM (BD Biosciences) following the manufacturer’s recommendations ...
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bioRxiv - Immunology 2024Quote: ... This was followed by fixation and permeabilization using either the FOXP3 staining buffer set (eBioscience, 00-5523-00) or the Cytofix/Cytoperm kit (BD, 554714). Intracellular staining was done using the following antibodies ...
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bioRxiv - Bioengineering 2024Quote: ... Samples requiring only intracellular cytokine staining were fixed for 20 min on ice using the BD Cytofix/Cytoperm kit (BD 554714) before washing and intracellular staining for at least 1 hr at 4°C ...
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bioRxiv - Immunology 2023Quote: ... ELISA for human IL-6 and IL-10 were performed using ELISA kits (DuoSet [R&D Systems] or BD OptEIA [BD]) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... 1.106 cells were incubated 20 min at 37°C in 100 μL solution BD cytofix/ cytoperm (BD PharmingenTM BrdU Flow Kit, 552598, BD Bioscience), washed with PBS and re-suspended in 700 µl of staining buffer (PBS with 3% SVF) ...
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bioRxiv - Immunology 2023Quote: ... and ionomycin (500 ng/mL) for 3 hrs at 37°C prior to surface and intracellular staining using the BD Cytofix/Cytoperm Kit (BD Biosciences). Intracellular transcription factor staining was performed using a Foxp3 staining kit (ThermoFisher) ...
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bioRxiv - Developmental Biology 2023Quote: ... The final cDNA library was generated from double strand full length cDNA by random priming amplification with the BD Rhapsody cDNA Kit (633773, BD Biosciences) and the BD Rhapsody Targeted mRNA & AbSeq Amplification Kit (633774 ...
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bioRxiv - Immunology 2023Quote: ... ELISA for human IL-6 and IL-10 were performed using ELISA kits (DuoSet [R&D Systems] or BD OptEIA [BD]) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... at least 2 x 106 cells were fixed and permeabilized using the Fixation/Permeabilized Solution Kit with BD GolgiStop TM (BD Biosciences) following the manufacturer’s recommendations ...
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bioRxiv - Bioengineering 2024Quote: ... and the hemoglobin contents were quantified after 8 h by the colorimetric assay kit (absorbance: 550 nm; with BD Pharm Lyse). Each value was subtracted from the internal control without the anti-BSA antibody.
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed with ice-cold FACS buffer (DPBS supplemented with 2% FBS and 2 mM EDTA) and subjected to fixation and staining with Cell Fixation and Permeabilization Kits (BD Biosciences). Incubation with antibodies was performed for 1h at RT ...
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bioRxiv - Immunology 2024Quote: ... and CD45.2+ peripheral blood leukocytes were FACS-sorted and labeled using the BD Rhapsody Single-Cell Multiplexing CTT Kit (BD Biosciences, 626545). On average ...
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“Identification of microRNAs regulated by E2F transcription factors in human pluripotent stem cells”bioRxiv - Developmental Biology 2024Quote: The distribution of cell populations throughout the cell cycle and the fraction of cells capable of incorporating BrdU were determined using the BrdU Flow Kit (BD Biosciences). After treatments cells were incubated with BrdU (10 ⎧M ...
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bioRxiv - Immunology 2022Quote: ... Brain cells isolated from Pba-infected mice injected with BrdU were stained using the FITC BD BrdU Flow Kit (BD Pharmingen™) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... cells were stained for surface markers (when required) then fixed/permeabilized by using PE Active Caspase-3 Apoptosis Kit (BD Bioscience, 550914) according to the manufacturer’s protocol ...
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PPARγ is a tumor suppressor in basal bladder tumors offering new potential therapeutic opportunitiesbioRxiv - Cancer Biology 2019Quote: ... The GAL4 DNA-binding domain cloning vector pM and the activation-domain cloning vector pVP16 are part of the Mammalian Matchmaker Two-Hybrid Assay kit (BD Biosciences Clontech). The construct pM-MED1 (510-787 ...
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bioRxiv - Cell Biology 2021Quote: ... The number of CD19 molecules expressed on the cell surface was estimated by flow cytometry in combination with the BD Quantibrite kit (BD Biosciences, #340495) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Cells were then washed 1 time with 100 µL PBS and fixed and permeabilized in 100 µL Fixation and Permeabilizaton Solution from the BD Cytofix/Cytoperm Fixation/Permeabilization Kit (BD BioSciences 554714) for 10 minutes at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Phagocytosis assay was carried out in heparinized whole blood samples incubated with fluorescein (FITC)-labelled opsonized Escherichia coli bacteria using the Phagotest kit (BD Pharmigen, Spain) as indicated in the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... QT3 and QT4 were processed by Flow Cytometric Analysis with a Cycletest PLUS DNA Reagent Kit (BD Biosciences, Le pont de Claix). Number of replicates per group are provided in Supplementary Table 1 ...
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bioRxiv - Immunology 2022Quote: ... Cells were then washed 1 time with 100 µL PBS and then fixed and permeabilized in 100 µL Fixation and Permeabilization Solution from the BD Cytofix/Cytoperm Fixation/Permeabilization Kit (BD BioSciences, 554714) for 10 minutes at 4°C ...
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bioRxiv - Physiology 2022Quote: Genome-edited hiPSCs were evaluated for expression of pluripotency markers using the BD Human Pluripotent Stem Cell Transcription Factor Analysis Kit (BD Biosciences, UK). In vitro differentiation efficiency was assessed by expression of stage-specific markers of definitive endoderm (CXCR4 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then washed twice with PBS and stained using a Propidium Iodide/RNase Staining kit (BD PharMingen. Franklin Lakes, NJ. USA) in the dark at room temperature for 30 min ...
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bioRxiv - Developmental Biology 2022Quote: ... and cell cycle analysis was performed by staining cells with BrdU-APC and 7-AAD with BD Pharmingen™ APC BrdU Flow Kits (552598, BD Biosciences), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Pro-inflammatory cytokines tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were measured in tissue culture supernatants using a commercially available sandwich ELISA kit (BD Biosciences). As recommended by the manufacturer ...
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bioRxiv - Pathology 2019Quote: ... and TNF-α) in the homogenate were measured using the CBA Mouse Th1/Th2/Th17 cytokine kit (BD Biosciences; CA, United States) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... The cell-free supernatants were assayed for IL-1β and TNFα using a commercial Enzyme-Linked Immunosorbent Assay (ELISA) Kit (BD Bioscience-Pharmingen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: Cell cycle was analyzed by flow cytometry with an allophycocyanin (APC) BrdU Flow Kit according to the manufacturer’s protocol (BD Biosciences, Mississauga, ON). Cells were labeled with bromodeoxyuridine (BrdU ...
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bioRxiv - Immunology 2019Quote: ... Unfractionated and lineage-depleted (Lin-) cells were labelled with phycoerythrin (PE) conjugated antibodies for CD11b and c-Kit (BD Biosciences, Mississauga, ON), and analyzed by flow cytometry ...
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bioRxiv - Genetics 2020Quote: ... 10 × 106 cells from villages of iPSCs or neurons were fixed and permeabilized using the Fixation/Permeabilization Solution Kit (BD Biosciences, 554714). Cells were resuspended in FACS buffer (1 x PBS ...
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bioRxiv - Immunology 2020Quote: ... Plasma TNF and IFN-γ levels were measured using their respective ELISA kits (BD OptEIA™; BD Biosciences, San Diego, CA, USA).
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bioRxiv - Cancer Biology 2022Quote: ... cells were fixed at 4 °C for 20 min and washed in permeabilization buffer from the Fix/Perm kit (BD Biosciences, 554714). Antibodies against intracellular targets were diluted in permeabilization buffer and cells were incubated at 4 °C for 30 min followed by another wash prior to assessment with BD LSR Fortessa SORP cytometer ...
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bioRxiv - Molecular Biology 2022Quote: ... The dermis was incubated in the enzyme mix from the whole skin dissociation kit (Milteney Biotec) for 3 hours according to the manufacturer’s instructions and further processed by Medicon tissue disruptor (BD Biosciences, Stockholm, Sweden). The dermal cell suspension was incubated with CD90 Microbeads ...
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bioRxiv - Immunology 2022Quote: ... cells were fixed and permeabilised using the BD Cytofix/Cytoperm™kit as per the manufacturer’s instructions (BD Biosciences, San Jose, CA) and stained with GATA3-PE (TWAJ ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed twice with 2% FACS buffer with 50nM dasatinib and fixed using fixation/permeabilization solution (Cytofix/Cytoperm™ fixation and permeabilization kit, BD Biosciences) for 20 minutes at 4°C ...
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bioRxiv - Synthetic Biology 2023Quote: ... A third of each cell sample was stained for 30 mins at 4°C with 1:100-dilutions of APC-conjugated anti-C-Kit/CD117 (BD Biosciences; 550412) and PE-Cy7-conjugated anti-CXCR-4/CD184 (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... cells were surface stained and then perforated and intracellularly stained with a BD fixation/permeabilization kit (BD Biosciences, San Jose, CA, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2023Quote: The concentration of cytokine IL-12 present in serum samples was evaluated using the “Cytometric Bead Array” kit (CBA, BD Bioscience, USA). The analyses of these cytokines were performed using flow cytometry and the data analyzed using FCAP Array software.
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bioRxiv - Immunology 2023Quote: ... IL-17 and TNF-α in BALF and blood sera of all the mice groups by utilizing commercially available kits brought from BD (USA), as per the manufacturer’s instructions.