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Citations for Becton, Dickinson and Company :
1451 - 1500 of 1655 citations for hsa mir 296 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: The release of IL-10 and IL-6 were quantified in cell supernatants using specific immunoassay kits purchased from BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... Cytokine production (IFN, IL-4, IL-17A, IL-10) was also assessed by Cytometric Bead Array (CBA) Kit (BD Biosciences) on supernatants collected at day 3 of polarization ...
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bioRxiv - Microbiology 2022Quote: ... Cells were fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization kit according to the manufacturer’s instructions (BD Biosciences). The presence of intracellular capsid protein VP1 was determined by staining with polyclonal rabbit anti-EV-D68 VP1 (10 µg/ml ...
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bioRxiv - Systems Biology 2023Quote: Brdu staining was used to analyze the cancer cell proliferation according to manufacturer the protocol (Brdu APC flow Kit, 552598, BD). Briefly ...
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bioRxiv - Immunology 2022Quote: ... the cells were analyzed by bromodeoxyuridine (BrdU) incorporation and DNA content staining with APC BrdU Flow Kit (BD Biosciences, #552598) per the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Intracellular cytokine staining was performed after 15 minutes fixation using BD Cytofix/Cytoperm™ Fixation/Permeabilization Kit (BD Biosciences, #554714). Flow cytometry data were recorded on BD LSRII or FortessaX20 using DIVA software and analyzed using FlowJo™ software (Tree Star).
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bioRxiv - Immunology 2023Quote: ... Intracellular expression of cytokines and transcription factors was analyzed using the respective fluorochrome-coupled mAbs in conjunction with either the BD Cytofix/Cytoperm kit (BD) or the Foxp3 staining buffer set (eBioscience ...
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bioRxiv - Immunology 2023Quote: ... mice were injected with 500μL BrdU (2mg/ml) intraperitoneally 12h prior to analysis using a BrdU flow kit (BD Biosciences) as previously 52 ...
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bioRxiv - Cell Biology 2023Quote: ... The BrdU pulse was followed by a 3-weeks chase period to detect by flow cytometry label-retaining cells by combining surface staining to define MPPs with intracellular staining using the BrdU-FITC labeling kit following the manufacturer’s instructions (BD Biosciences). Apoptosis was measured using the Annexin V detection kit (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... permeabilised with Foxp3 transcriptional factor staining buffer kit (eBioscience) before intracellular staining with appropriate cytokine antibodies and acquisition through LSR Fortessa machine (BD Immunocytometry system ...
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Mice generated with induced pluripotent stem cells derived from mucosal-associated invariant T cellsbioRxiv - Immunology 2023Quote: ... the cells were subjecte to intracellular staining with BD Cytofix/Cytoperm buffer kit according to the manufactures’s instruction (BD BioScience). Cells were analyzed with the MACSQuant cell analyzer (3 lasers ...
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bioRxiv - Bioengineering 2022Quote: ... and MHCII-NYESO conjugated on the SCMs are quantified by the PE Phycoerythrin Fluorescence Quantitation Kit (BD Biosciences, Cat # 340495). Each test in the kit comes with 4 different intensities of PE-labeled beads ...
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bioRxiv - Immunology 2023Quote: ... 1 x 105 HSC-F cells were infected with SIVmac239 at MOI 0.05 in U-bottomed plates and permeated with Cytofix/Perm kit (BD Biosciences). After two washes ...
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bioRxiv - Immunology 2023Quote: ... Intracellular staining for cytoplasmic and nuclear proteins were performed with Transcription Factor Staining Buffer kit according to manufacturer’s instructions (BD Pharmingen). Dead cells were excluded by DAPI (BioLegend ...
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bioRxiv - Immunology 2023Quote: Phagocytic potential of blood-derived monocytes and granulocytes was examined immediately after necropsy by Phagotest and Phagoburst kits (BD Biosciences). Phagocytosis was examined by stimulating blood samples with opsonized E ...
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bioRxiv - Microbiology 2023Quote: ... the supernatants were collected for cytokine determination using ELISA kits according to the manufacturer’s recommendations (R&D and BD Bioscience).
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bioRxiv - Microbiology 2023Quote: ... The cytokine level from the plasma was quantitated using cytometric bead array kit (P110100403, JiangXi Cellgene) on Flow Cytometry (FASCantoII, BD). All measurements were handled according to the manufacturer instruction ...
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bioRxiv - Cell Biology 2023Quote: Cells were analyzed for cell cycle phase using a ClickIT EdU Alexa Fluor 647 kit (Fisher) and FxCycle PI/RNase Staining Solution (Fisher) followed by flow cytometry on an LSR-II (BD) and analysis using FlowJo.
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bioRxiv - Cancer Biology 2024Quote: ... and whole-transcriptome and Sample-tag libraries were obtained using the BD WTA amplification kit and protocol (BD Biosciences, #633801). The quality of the final libraries was assessed by using an Agilent 2200 TapeStation with HS D5000 ScreenTape (Agilent Technologies ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Infection was monitored by flow cytometry at 72 hrs post infection to assess GFP (unfixed cells) or TRIM5α-HA expression (using the CytoFix/Cytoperm kit [BD] according to manufacturer’s instructions and staining with anti-HA-PE [Biolegend] diluted 1:200) ...
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bioRxiv - Genomics 2024Quote: ... RoCKseq beads were resuspended in 680 µl Sample Buffer (Cat. No. 650000062, BD RhapsodyTM Enhanced Cartridge Reagent Kit, BD 664887) instead of 750 µl.
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bioRxiv - Cell Biology 2020Quote: ... and macrophage chemoattractant protein-1 (MCP-1) were quantified by bead-based flow cytometry assay (CBA Kit; BD Biosciences, Heidelberg, Germany) in accordance with the instructions of the manufacturer.
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bioRxiv - Immunology 2021Quote: ... Cells for intracellular staining of HLA-DR were first fixed and permeabilised using BD Cytofix/Cytoperm™ Plus Fixation/Permeabilization Kit (BD Biosciences ...
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bioRxiv - Microbiology 2020Quote: Macrophages were infected in a 48-well plate as described above and harvested supernatants were assayed for cytokine levels using ELISA kits for human IL-1β (BD Biosciences) and IL-18 (R&D Systems).
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Genetically engineered microglia-like cells have therapeutic potential for neurodegenerative diseasebioRxiv - Neuroscience 2021Quote: ... and with hematopoietic stem cell markers APC-conjugated anti-CD117 (c-Kit) and PE-conjugated Ly6A/E (Sca-1) (BD Biosciences). Lineage negative cells were transduced with a lentiviral vector in a cell incubator at 2 ×106 cells/mL in either StemSpan SFEM II (STEMCELL Technologies ...
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bioRxiv - Immunology 2021Quote: ... primary hMDMs were transfected in a 48-well plate as described above and harvested supernatants were assayed for cytokine levels using an ELISA kit for human IL-1β (BD Biosciences) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2021Quote: The mode of cell death was analyzed by flow cytometry with an Annexin V-FITC and propidium iodide (PI) double-staining kit (BD Biosciences). After the treatments ...
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bioRxiv - Bioengineering 2019Quote: ... with anti-positive (CD90, CD105, CD44) and negative marker (CD34, CD11b, CD19, CD45, MHC class II) antibodies (Human MSC Analysis Kit, 562245, BD Biosciences), as described in detail in [42] ...
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bioRxiv - Immunology 2019Quote: ... cells were cultured in the presence of 1 μg/mL Brefeldin A for indicated timepoints and were fixed and permeabilized with Cytofix/Cytoperm kit (BD Biosciences) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2019Quote: Cytometric bead array (CBA) was performed using the mouse soluble protein master buffer kit combined with the appropriate CBA flex sets (BD Biosciences), per manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... We then double sorted lineage depleted cells for an HSC-enriched population (Lin- Sca-1+c-Kit+CD34+-CD135-) into 2% FBS in HBSS using fluorescent activated cell sorting (FACS) (BD Aria). Recipient mice ...
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bioRxiv - Bioengineering 2020Quote: ... Cytokine concentrations in the culture supernatant were measured using BD Cytometric Bead Array Human Th1/Th2 Cytokine Kit II (BD Biosciences).
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bioRxiv - Immunology 2021Quote: ... Cytokines in the serum sample were measured using human Th1/Th2/Th17 cytometric bead array kit (BD Bioscience, San Jose, CA) according to the manufacture’s instruction ...
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bioRxiv - Bioengineering 2020Quote: ... Intracellular staining for the S protein was performed for 60 min on ice after using a fixation/permeabilization kit (eBioscience) and then analyzed using a FACSCalibur flow cytometer (BD Biosciences). The phagocytic score was determined by gating the samples on events representing cells and was calculated as follows ...
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bioRxiv - Immunology 2020Quote: ... and cells were fixed and permeabilized at room temperature for 15 min with Fixation/Permeabilization Solution Kit (BD Biosciences; cat. #554714). For each sample a minimum of 1.2x105 stopping gate events (live CD3+ T-cells ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were trypsinized, filtered through a 70 μm strainer (Laboteca; McAllen, TX) and fixed/permeabilized using the Cytofix/Cytoperm Solution Kit (BD Biosciences), according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: An amount of 100 μl serum was placed in 96-well plates to assess total IgE content with an IgE-specific enzyme-linked immunosorbent assay (ELISA) kit (BD Pharmingen) [10] ...
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bioRxiv - Cell Biology 2021Quote: ... GMPGFP+ and GMPGFP- or clonal HoxA9 cells were sorted directly in the fixation buffer from the Fixation/Permeabilization kit (BD Biosciences). Cells were then washed with PBS supplemented with 3% BSA followed by the Click-iT reaction as described above ...
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bioRxiv - Cell Biology 2020Quote: ... intracellular staining with anti-FoxP3-FITC antibodies (clone: FJK-16s; Thermo Fischer Scientific) was performed with the BD Cytofix/Cytoperm Kit (BD Biosciences) according to the manufacture instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... treated with 75µM cisplatin and stained with Annexin V conjugated to Alexa Fluor 488 dye and propidium iodide (PI) using Dead Cell Apoptosis Kit (BD Biosciences) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... then cell culture supernatant were harvested and analysed using a BD Cytometric Bead Array (CBA) Human Soluble Protein Master Buffer Kit (BD Biosciences) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: Concentrations of TNF-α and IFN-γ in sera on day 21 after allogeneic BMT were measured with specific anti-mouse ELISA kits from BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... TNF-α and IFN-γ in the serum of experimental monkeys were detected with the Non-Human Primate (NHP) Th1/Th2 Cytokine Kit (BD) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... The enzyme activity was stopped with fetal bovine serum (FBS) followed by the fixation and staining with the APC BrDU Flow Kit – Part A (552598, BD Pharmingen) according to the manufacturer’s recommendation with some exceptions ...
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bioRxiv - Immunology 2022Quote: ... Intracellular staining was performed after treatment with Fixation/Permeabilization solution and in the presence of Perm/Wash buffer (Cytofix/ Cytoperm kit, BD Biosciences) using anti-mouse IFN-γ FITC ...
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bioRxiv - Immunology 2022Quote: ... The Foxp3 staining kit was used for intracellular transcription factor staining and the Cytoperm/Cytofix Plus staining kit was used for cytokine staining (BD Biosciences). Data was analyzed using FlowJo (TreeStar) ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed twice with 500 μL permeabilization buffer (from kit described above) and stained with 150 μL permeabilization buffer containing 1:400 Ki67-Alexa-Fluor antibody (561126, BD Biosciences) for 30 minutes at 4 °C ...
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bioRxiv - Immunology 2019Quote: ... Intracellular cytokine staining was performed to examine the production of cytokines in these CAR-T cells using Fixation/Permeabilization Solution Kit (BD Biosciences). CD107 expression was determined as described (Liu et al. ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... IL-12 and IL-6 was simultaneously analyzed by flow cytometry (FACS Canto II; Becton-Dickinson) using the BD Cytometric Bead Array (CBA) Human Inflammatory Cytokines Kit (BD™) according recommendations ...
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bioRxiv - Immunology 2019Quote: Cell surface staining was performed as described in the protocol “Single Cell Labelling with the BD Single-Cell Multiplexing Kit and BD AbSeq Ab-oligos” (BD Biosciences). Briefly ...