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Citations for Becton, Dickinson and Company :
1451 - 1500 of 1548 citations for Tryptic Soy Agar TSA CRITERION Dehydrated Culture Media since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... and then 10 mL was dispensed into each sterile tissue culture dish (BD Falcon, 100 mm diameter x 20 mm depth) and incubated at 37°C with 5 % CO2 for 24 h ...
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bioRxiv - Molecular Biology 2024Quote: ... and also to eliminate the presence of mitomycin-C-treated MEFs (used as feeders during our mESC cultures) we performed FACS (Fluorescence-Activated Cell Sorting) using a FACSAria cell sorter (BD Biosciences). The dissociated mESC were first labeled with the eBioscienceTM Fixable Viability Dye eFluorTM 780 (ThermoFisher ...
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bioRxiv - Microbiology 2024Quote: ... Colonies isolated from growth on Columbia sheep blood agar plates were used to inoculate mNYC III media and cultures were incubated for 48 hours at 37°C under anaerobic conditions (GasPak™ EZ Anaerobe Container System Sachets, BD).
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bioRxiv - Immunology 2024Quote: ... sorted T3a and T3b B cells were assayed for differentiation to FO at 24 hours in culture by flow cytometry using MitoTracker staining in conjunction with the following primary antibody panel: CD38 (HIT2, BD Biosciences), CD24 (ML5 ...
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bioRxiv - Cell Biology 2024Quote: ... Cleaned coverslips were removed from 70% ethanol and allowed to dry in a biosafety cabinet then were placed into the wells of a 6 well tissue culture plate and coated with 10 µg/ml fibronectin (BD Biosciences) in PBS (10.1 mM Sodium phosphate dibasic ...
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bioRxiv - Developmental Biology 2024Quote: ... The PDMS chip (soaked in PBS) and the culture medium (filled in 10 mL syringes; BD Biosciences, 300912, diameter 14.5 mm) were degassed before use for at least one hour in a vacuum desiccator chamber.
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bioRxiv - Developmental Biology 2024Quote: All cell culture of all species was maintained at 37°C with 5% CO2 and 5% O2 on Matrigel (1:100, BD Corning) coated plates unless otherwise specified ...
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bioRxiv - Genomics 2024Quote: ... day 7 cells were transferred to fresh culture medium with IL-2 and stimulated again with peptides and 1ug/mL of anti-human CD28 antibody (BD Biosciences) for another 7 days ...
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bioRxiv - Bioengineering 2023Quote: ... At the end of the co-culture the cells were washed twice and incubated with human or mouse Fc receptor blocking antibodies (BD Biosciences). Human macrophages were stained with anti-CD11b (M1/70 ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 x 106 dissociated organoid cells in 100 µl hepatocyte culture medium and 5% Matrigel were injected subcutaneously into the flank using a 1 ml syringe with 25G needle (BD 305122). For human grafts ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were dissociated using Trypsin-EDTA and BFP-positive cells were individually seeded into wells of a 96-well plate for single-cell culture by fluorescence-activated cell sorting (FACS) using BD FACSAria III (BD Biosciences). As described by Zhang et al ...
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bioRxiv - Cell Biology 2022Quote: ... 5 × 106 MCF-7 cells stably expressed shCtrl and shMYSM1 lentivirus were suspended in 100 μl of culture medium/Matrigel (1:1) (BD Biosciences) and implanted subcutaneously into the bilateral armpits of 4-week-old female BALB/c nude mice (Vital River Laboratory) ...
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bioRxiv - Cell Biology 2023Quote: ... 100 μl of this suspension (1×10e5) was transferred to a 5 ml culture tube and 5 μl of FITC Annexin V (BD Biosciences) added ...
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bioRxiv - Biophysics 2022Quote: THP-1 cells were cultured in wells as detailed in the cell culture methods and analyzed using a flow cytometer (BD Accuri C6 flow cytometer, BD Biosciences). Differentiated cells were detached using Enzyme Express (1X ...
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bioRxiv - Cancer Biology 2023Quote: ... The percentage of sgRNA transduced GFP-positive cell population in culture was measured at indicated time points using a LSRII flow cytometer (BD Biosciences). The change of GFP percentage was used to assess proliferation of sgRNA-transduced cells relative to the non-transduced cells in the culture.
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bioRxiv - Immunology 2023Quote: ... Cytokine concentrations in the supernatants of DC cultures were measured using a BD human inflammatory cytometric bead array kit (BD #551811) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... cells were diluted with 37°C pre-warmed cell culture medium and analyzed with a MaxQuantX Flow Cytometer (Miltenyi Biotech) or BD FACSAria Fusion Flow cytometer (BD Biosciences). After acquisition of the baseline fluorescence level ...
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bioRxiv - Cancer Biology 2023Quote: ... Live cells from each sample were sorted into one 96-well flat-bottom low attachment tissue culture plate well containing 30 μl sorting buffer on a cold plate using Aria II flowcytometry cell sorter (BD Biosciences) with a 70 µm nozzle (70 psi) ...
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bioRxiv - Cell Biology 2023Quote: Skin equivalent cultures using a Collagen:matrigel gel were prepared by mixing 4.9 volumes of type I collagen (BD Biosciences, Oxford, UK), 2.1 volumes of Matrigel (BD Biosciences) ...
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bioRxiv - Cell Biology 2023Quote: ... Single cells from mosaic cultures were sorted directly into individual wells of a 96 well plate using a BD FACS Jazz (BD Biosciences) and cultured to form colonies over 2-3 weeks ...
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bioRxiv - Cell Biology 2023Quote: The EBs were dissociated as described above and the cells were plated onto 24 well culture dishes pre-coated with matrigel (25% v/v, BD PharMingen). Cells were cultured for 2–3 days and were fixed with 4% PFA in PBS for 15 min at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: Live cells were incubated with 100 nM 6-FAM-dC-puromycin in complete culture medium for 30 min and analyzed by a FACSCalibur flow cytometer (BD Biosciences).
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bioRxiv - Immunology 2024Quote: ... Cell culture supernatants were assayed for IL-2 using capture and biotin-labeled detection antibody pairs (BD Biosciences, Franklin Lakes, NJ). Detection was performed with HRP-conjugated streptavidin (BD Biosciences ...
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bioRxiv - Microbiology 2024Quote: ... overnight cultures were either grown with aeration (180 rpm, Infor shaker) in case of ETEC or anaerobically (GasPak™ EZ Systems, BD) in case of B ...
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bioRxiv - Cell Biology 2024Quote: ... and Exo-BLD exosomes derived from cancer cell line culture mediums and serum using both the conventional flow cytometer (BD FACSCelesta) and the imaging flow cytometer (VIFFI flow cytometer) ...
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bioRxiv - Bioengineering 2024Quote: ... IL-2 levels in the co-culture supernatant were quantified using ELISA (BD OptiEIA Human IL-2 ELISA kit, BD Biosciences) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... of pressurized culture were taken using a 1 mL Injekt-F syringe (Braun) fitted with a 23-gauge sterile needle (BD Microlance) by puncturing the rubber fitting ...
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bioRxiv - Cell Biology 2024Quote: ... floating hMG progenitors were collected from the culture’s supernatant and passed through a 70 μm Filcon™ Syringe-Type nylon mesh (BD Biosciences) to ensure a single-cell suspension and to remove cell clumps ...
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bioRxiv - Immunology 2024Quote: ... was added at the start of the co-culture and GolgiStop as well as GolgiPlug (containing monensin and brefeldin A, respectively, both BD Biosciences) were added after 1 h ...
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bioRxiv - Cancer Biology 2024Quote: Cells were harvested from cell culture dishes at the indicated experimental conditions and resuspended in 100 μL Annexin V Binding buffer (BD Biosciences) at a density of 2 × 105 cells/mL ...
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bioRxiv - Cancer Biology 2024Quote: ... and fresh whole blood was collected on the day of co-culture for each timepoint into BD Vacutainer tubes coated with EDTA (BD #367525). PBS was added to the whole blood collected in EDTA coated vacutainer tubes at equal volumes ...
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bioRxiv - Microbiology 2021Quote: ... and several ten-fold dilutions (10−4 to 10−7) were plated onto de Man Rogosa and Sharpe (MRS) media (BD Difco, USA) supplemented with 50 mg/L of mupirocin (PanReac AppliChem ...
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bioRxiv - Cell Biology 2022Quote: ... washed with PBS and incubated in DMEM media containing 1% FBS and 1% BSA with Alexa Fluor® 647 Mouse Anti-Human LAMP1 Clone H4A3 (BD Biosciences) for 1h at 4°C with intermittent mixing ...
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bioRxiv - Microbiology 2022Quote: ... HFK were maintained in E media containing 5% FBS and 5 ng/ml of mouse epidermal growth factor (EGF, BD Biosciences; 354010) and 10 µM Rho kinase inhibitor (ROCK ...
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bioRxiv - Microbiology 2022Quote: ... HFKs were cultured in E media containing 5% FBS and 5 ng/ml of mouse epidermal growth factor (EGF, BD Biosciences; 354010) and 10 μM Rho kinase (ROCK ...
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bioRxiv - Microbiology 2022Quote: ... These cells were cultured in E media containing 5% FBS and 5 ng/ml of mouse epidermal growth factor (EGF, BD Biosciences; 354010) and with NIH 3T3 J2 fibroblast added as feeders ...
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bioRxiv - Cancer Biology 2020Quote: ... single cells positive for tdTomato were sorted under sterile conditions into 384-well plates containing OCI-C5x-conditioned media (50 µl/well) using fluorescence-assisted cell sorting (FACSAria cell sorter; BD Biosciences, Inc.). OCI-C5x conditioned media was prepared by seeding 7.5×105 OCI-C5x cells in a 10cm plate with 10ml OCMI-L media and collected 48 hours after incubation ...
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bioRxiv - Microbiology 2020Quote: ... The fundamental M9 media formulation (pH 7) was supplemented with 0.8% deferrated acid-hydrolyzed casamino acids (BD Biosciences San Jose, CA, USA), 8 mM MgSO4 ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were incubated in media containing 10 µg/mL blocking antibody (Purified Rat Anti-Human CD29 Clone Mab 13, BD Biosciences, #552828) or equivalent concentration non-targeting isotype control (Purified Rat IgG2a κ Isotype Control Clone R35-95 ...
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bioRxiv - Immunology 2024Quote: ... and Parabacteroides distasonis were grown anaerobically at 37°C in brain heart infusion (BHI) media (Oxoid, UK) supplemented with hemin (5 mg/L) and vitamin K (0.5 mg/L) (BD, Franklin Lakes, NJ). Lactobacillus johnsonii and Ligilactobacillus murinus were grown anaerobically at 37°C in de Man Rogosa Sharpe (MRS ...
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bioRxiv - Genetics 2023Quote: ... Primed H9 cells were maintained in HFF-conditioned media (HFF-CM) supplemented with 8 ng/ml of β-FGF (MILTENYI BIOTE) on Matrigel (BD, Biosciences) coated surfaces cell lines in a humidified 5% CO2 incubator at 37ºC ...
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bioRxiv - Cell Biology 2022Quote: ... 100,000 PBMCs that had been dissolved and quantified in each media were stained with antibodies for CD3 (APC-H7, BD Biosciences (BD)) ...
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bioRxiv - Synthetic Biology 2023Quote: ... 2% dextrose) when containing no plasmids and in synthetic defined (SD) media (0.67% yeast nitrogen base without amino acids [BD Difco], 2% dextrose) supplemented with the appropriate yeast synthetic drop-out medium supplement when plasmid maintenance was required (SDΔW-without tryptophan ...
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Langerhans cells regulate immunity in adulthood by regulating postnatal dermal lymphatic developmentbioRxiv - Immunology 2024Quote: Cells isolated from crawl-out epidermal cultures were washed with PBS and rested in serum-free media with 2 x 105 cells per well in 96-well assay plate (BD falcon, #353910) for 30 min ...
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bioRxiv - Systems Biology 2024Quote: ... and lawns of pAWP78-P89-20ZRfae in E.coli S17-1 (overnight) were prepared for mating on plates with Hypho media supplemented with 5% Nutrient Broth (NB Difco, BD Life Sciences). Plates were incubated for 1 day at 30° C ...
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bioRxiv - Bioengineering 2024Quote: ... cells were incubated with AF647-anti-VE-cadherin antibody (1:200, v/v, diluted in pre-warmed EGM-2 media, BD Biosciences, #561567) for 30 minutes orbital shaking ...
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bioRxiv - Cancer Biology 2021Quote: ... For start of the migration fresh cell culture medium was added with or without 10 μg/ml 9EG7 antibody (BD Biosciences, #553715). The progress of migration was recorded every minute with temperature and CO2 controlled Leica DM IL LED microscopes with Leica HI PLAN I 10x/0.22 air objectives ...
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bioRxiv - Bioengineering 2020Quote: Jurkat activation during anti-CD3 administration or co-culture was assessed using flow cytometry detection of CD69 with an APC-conjugated anti-CD69 antibody (BD Bioscience 555533). Cells were not washed prior to or after staining ...
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bioRxiv - Immunology 2022Quote: Pro-inflammatory cytokines tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were measured in tissue culture supernatants using a commercially available sandwich ELISA kit (BD Biosciences). As recommended by the manufacturer ...
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bioRxiv - Cancer Biology 2022Quote: ... Transduced cells were then expanded further in culture and FACS sorted for mStrawberry expression (BD-FACS Aria, BD Bioscience, Franklin Lakes, NJ).