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Citations for Becton, Dickinson and Company :
1451 - 1500 of 8130 citations for Protein Kinase A PKA Activity Assay Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Electroporated cells were cultured in 24-well plates for 5-7 days and transferred to 6-well plates prior to selection by fluorescence-activated single cell sorting using FACS Melody (BD Biosciences).
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bioRxiv - Cell Biology 2024Quote: ... Cleaned coverslips were removed from 70% ethanol and allowed to dry in a biosafety cabinet then were placed into the wells of a 6 well tissue culture plate and coated with 10 µg/ml fibronectin (BD Biosciences) in PBS (10.1 mM Sodium phosphate dibasic ...
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bioRxiv - Immunology 2024Quote: ... and 5×104 of each were combined in 96-well U-bottom plates with anti-CD40 (100 ng/mL, clone HM40-3, BD Biosciences) in the presence or absence of OVA (10 μg/mL ...
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bioRxiv - Microbiology 2024Quote: ... overnight cultures of each strain were grown in BHI media and spot plated on agar plates made from tryptic soy broth without added dextrose (TSB-D, BD Difco) supplemented with 3% (wt/vol ...
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bioRxiv - Microbiology 2024Quote: Single cells were sorted into 384-well plates for SL-Smart-seq3xpress library preparation by flow cytometry using a FACS Fusion II cell sorter (BD Biosciences) and a 100µm nozzle within a safety cabinet ...
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bioRxiv - Biophysics 2024Quote: ... single cells were gated to include low sfGFP signal and sorted into 96-well plates on BD FACSAria Fusion (BD Biosciences) at the Flow Cytometry Resource Facility at Princeton University ...
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bioRxiv - Cell Biology 2024Quote: The human iPSC line MUSli001-A was generated from skin fibroblasts from a caesarean section [34] and cultured on a polystyrene plate coated with 2 µg/mL Matrigel (Growth Factor Reduced; Corning, BD Bioscience) at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2022Quote: FACS-sorted Tregs were plated at 50,000 cells/well in round bottom 96 well plates pre-coated with anti-human CD3 (2 μg/ml, clone UCHT1, BD Biosciences) and soluble anti-human CD28 (1 μg/ml ...
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bioRxiv - Microbiology 2022Quote: ... 10 µl of this bacterial suspension was added to the wells of a sterile 96-well flat-bottomed plate and incubated with 90 µl of freshly-donated human blood (collected in EDTA-treated tubes; BD Biosciences). Blood from each of four individual volunteers was assayed twice ...
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bioRxiv - Cell Biology 2023Quote: ... Jurkat cells were cultured for 3 days after electroporation and sorted into 96-well plates for clonal expansion using fluorescent signal to isolate edited clones using the BD Bioscience Influx sorter (BD Bioscience). Both alleles of AP-2 and one allele of DNM2 were tagged in the final cell line.
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bioRxiv - Neuroscience 2023Quote: ... Single GFP-positive cells were sorted into individual wells of vitronectin-coated 96-well plates using a FACSJazz cell sorter (BD Biosciences). The single cells were allowed to expand into single-cell derived colonies by culturing for 48 hours (incubated at 37°C in 95% humidity with 5% CO2 ...
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bioRxiv - Microbiology 2023Quote: ... and Sharpe (MRS) plates supplemented with 1.5% agar at 37°C in anaerobic conditions (BD GasPak™ EZ Anaerobe Container System) overnight ...
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bioRxiv - Genetics 2023Quote: ... For each reporter cell line single cells were FACS-sorted into 96-well plates using a FACSAria III cell sorter (BD Bioscience) to obtain single-cell derived clones ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were dissociated using Trypsin-EDTA and BFP-positive cells were individually seeded into wells of a 96-well plate for single-cell culture by fluorescence-activated cell sorting (FACS) using BD FACSAria III (BD Biosciences). As described by Zhang et al ...
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bioRxiv - Biophysics 2023Quote: ... Single cell clones of Cas9 and sgKRAS-transduced cells were sorted into 96-well plates using a FACSMelody benchtop sorter (BD Biosciences) and verified for successful knock-out of KRas by Western blot analysis and PCR amplicon sequencing ...
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bioRxiv - Cancer Biology 2023Quote: C4-2 cells were cultured and lifted from plates by trypsinization then re-suspended in 100 mL of 50% Matrigel (BD Biosciences) and saline mixture followed by subcutaneous injection in two separate groups of castrated and non-castrated athymic nude mice (age at least 6 weeks old) ...
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bioRxiv - Genomics 2022Quote: ... single cells expressing GFP (expressed from the CRISPR plasmid) were sorted into 96-well plates through flow cytometry using a BD FACSAria Fusion (BD Biosciences). Single-cell clones were cultured and tested for successful knockout of RFX7.
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bioRxiv - Immunology 2023Quote: ... cells were stained using the same protocol and then sorted in 96 plates using the ultra-purity mode on a MA900 (SONY) or an Aria II cell sorter (BD Biosciences) and Data were analyzed using FlowJo or Kaluza softwares ...
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bioRxiv - Cancer Biology 2023Quote: GBM or MCF10A cells were seeded at a density of 2000-4000 cells/well in a 96-well tissue-culture polystyrene plate (BD Falcon) that was pretreated with 3 μg/cm2 laminin for GBM cells (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... Anaerobic bacteria colonies were counted after incubating these agar plates for 2 days using the GasPak anaerobic gas generating pouch system (BD Biosciences).
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bioRxiv - Systems Biology 2023Quote: ... GM25256, Coriell) and hESCs (WA09, WiCell, Madison WI) were maintained under feeder-free conditions on Matrigel-coated plates (#354277, BD Biosciences) in mTeSR™ Plus (#100-0276 ...
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bioRxiv - Microbiology 2023Quote: ... GFP-positive cells were sorted and plated in a 96-well plate for single-clone selection with Becton Dickinson FACS Aria Fusion (BD Science). SAMHD1 deficiency was tested by Interferon-α stimulation and subsequent western blotting for SAMHD1 ...
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bioRxiv - Cancer Biology 2023Quote: ... We sorted single nuclei in 96-or 384-well plates (one nucleus per well in 10 nL sorting volume) using the BD FACSJazz Cell Sorter (BD Biosciences) based on forward and side scatter properties ...
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bioRxiv - Cell Biology 2023Quote: ... The cell suspension was transferred to a 24-well plate coated with 30 µl of Matrigel (growth factor reduced, phenol red-free; BD Biosciences). After 24 h ...
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bioRxiv - Cancer Biology 2023Quote: ... the MSCs and ALL cells were removed from the plate by trypsinisation and sorted on expression of CD90 using anti-CD90 FITC or anti-CD90 APC (BD Biosciences) and lack of expression of CD19 using anti CD19 APC or CD19 BV605 (BD Biosciences ...
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bioRxiv - Cell Biology 2023Quote: ... Single cells from mosaic cultures were sorted directly into individual wells of a 96 well plate using a BD FACS Jazz (BD Biosciences) and cultured to form colonies over 2-3 weeks ...
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bioRxiv - Bioengineering 2023Quote: ... VA) was cultured on plates of BBL Trypticase Soy Agar with 5% sheep blood (BD Trypticase Soy Agar II) (BD Biosciences) in a 37°C humidified 5% CO2 environment for 10-11 hours and diluted in 0.9% sodium chloride to the desired optical density ...
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bioRxiv - Microbiology 2023Quote: ... fragilis NCTC 9343 ΔPSA44 (lacks PSA biosynthesis locus) maintained in 25% glycerol at −80 °C were thawed on Brain Heart Infusion agar plates (BHI, BD BBLTM) supplemented with 5 µg/ml hemin (Alfa Aesar ...
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bioRxiv - Immunology 2023Quote: ... the plate was washed 5x with PBST 0.05% and incubated with biotinylated antibody G1 (Serotec MCA336B) or G2A (BD bioscience 553388) for 60min at RT ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were afterward sorted as single cells in 96-well plates for bright sfGFP and mOrange2 signals using a FACS Aria (BD Biosciences) cell sorter ...
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bioRxiv - Genomics 2024Quote: ... Single cells were sorted in single wells of a 96 well plate at the Cytometry Facility at the University of Zürich using a BD S6 5L cell sorter (BD Biosciences, Switzerland). The cell lines were then expanded and cultured as described above.
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bioRxiv - Cell Biology 2024Quote: ... mEGFP-negative and tagRFP-T-positive hiPSCs were pooled into Matrigel-coated plates using a BD Bioscience Influx sorter (BD Bioscience), three days after electroporation (Lonza ...
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bioRxiv - Molecular Biology 2024Quote: ... GFP- (PX458) or mRuby-positive (PX461) cells were single cell sorted into 96-well plates using a FACSAria™ III sorter (BD).
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bioRxiv - Systems Biology 2024Quote: - selection of diploids twice on SC(MSG)-Ura + G-418 plates (1.7 g/l yeast nitrogen base without amino acids and ammonium sulfate (BD Biosciences, 233520), 1 g/l monosodium glutamic acid (MSG ...
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bioRxiv - Cell Biology 2024Quote: ... RKO-DOX-Cas9-P2A-BFP cells117 were transduced with pLX303-SFFV-MYC-mCherry-A3H-II-P2A-OLLAS-EGFP-A3H-I and mCherry+/GFP+ double-positive cells were sorted by FACS into 96-well plates using a FACSAria III cell sorter (BD Biosciences). The mutREAD cell line was generated by co-transducing pLX303-SFFV-MYC-mCherry-P2A-3xHA-A3H-I and DualCRISPR-hU6-sgUNG2-mU6-sgUNG2-Thy1.1-P2A-Neo into RKO-DOX-Cas9-P2A-GFP44 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells expressing GFP or Halo (labeled by JF549-HaloTag ligand) were subjected to single-cell sorting into 96-well plates 24-36 h after transfection (FACSAria II, BD Biosciences). Monoclonal cell populations with mutations in both alleles were identified through sequencing ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were afterward sorted as single cells in 96-well plates for bright sfGFP and mOrange2 signals using a FACSAria (BD Biosciences) cell sorter ...
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bioRxiv - Systems Biology 2024Quote: - N-SWAT and C-SWAT libraries were assembled in 384-colony format on SC-Ura plates (6.7 g/l yeast nitrogen base without amino acids (BD Biosciences, 291940), 2 g/l amino synthetic complete acid mix lacking uracil (SC-Ura) ...
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bioRxiv - Microbiology 2024Quote: ... the mixture was then transferred to the surface of 10-cm square 1,5% bottom agar plates (BD Difco 2216 marine broth) and incubated for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: Trans-well assay was done in 24-well Boyden chambers with 8-μm pore size polycarbonate membranes (BD Biosciences, San Diego, USA). ST1 cells (5 × 104 ...
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bioRxiv - Neuroscience 2019Quote: ... mIL-6 levels were evaluated using sandwich capture ELISA assays using the RIPA soluble mouse forebrain lysates as per manufacturer’s instructions (BD OptiEIA, BD Biosciences).
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bioRxiv - Immunology 2021Quote: Testing for GC and CT in first-void urine samples was performed by nucleic acid amplification test (NAAT; ProbeTech Assay, BD, Sparks, MD). Testing for HIV-1/2 and syphilis were performed by chemiluminescent microparticle immunoassay (CMIA ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were analyzed for GFP+ frequencies by flow cytometry as for the other reporter assays and/or used for cell sorting of GFP-negative (GFP-neg) cells (BD Aria sorter).
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bioRxiv - Cancer Biology 2021Quote: ... the BD Pharmingen APC BrdU Flow Kit and the PE Annexin V Apoptosis Detection Kit II were from BD Biosciences ...
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bioRxiv - Microbiology 2019Quote: ... Culture of 10ul from dilutions 1:10-1 - 1:10-8 was performed on CHROMagar Orientation (BD) and ESBL Brilliance agar (Oxoid) ...
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bioRxiv - Cancer Biology 2024Quote: ... and CAPAN-1 cells diluted in 200 μl of a 1:1 mixture of Matrigel (BD Biosciences) and PBS (Corning) ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-AIM-1 (611082, BD transduction laboratories, 1:200); mouse anti-α-Tubulin (DM1A ...
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bioRxiv - Cell Biology 2020Quote: ... This cell suspension was mixed 1:1 with matrigel (BD) and 100 μl were injected subcutaneously into a minimum of six male immunodeficient NOD/ShiJic-scid Jcl (NOD-SCID ...
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bioRxiv - Physiology 2022Quote: ... at 1:400 and Sca-1 V500 (BD Horizon, 561228) at 1:300 ...
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bioRxiv - Cell Biology 2022Quote: anti-Dynamin 1/2/3-mouse (BD Science, 1:1000), anti-Pacsin2-Rabbit (Proteintech ...