Labshake search
Citations for Becton, Dickinson and Company :
101 - 150 of 220 citations for SH3TC1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2022Quote: ... Single cell suspensions stained in PBS + 2% FBS in the presence of Fc receptor blocking antibody (BD Pharmingen) and stained with the antibodies against the following mouse proteins ...
-
bioRxiv - Immunology 2023Quote: ... the blocking buffer was aspirated and the slides were stained in 1:75 anti-CD35 (740029, BD Biosciences) primary antibody solution also made in blocking buffer for ∼16 hours at 4°C ...
-
bioRxiv - Physiology 2022Quote: ... and BLCs (PE Mouse Anti-NKX6.1: 1:40, BD Biosciences Cat# 563023, RRID:AB_2716792; and AF647 Mouse Anti-C-Peptide: 1:200 BD Biosciences Cat# 565831 ...
-
bioRxiv - Immunology 2023Quote: ... PBMC were co-cultured with peptides at 37°C for 6h with CD107a BV786 (BD Biosciences, H4A3) and 1μg/mL of CD28/CD49d costimulatory reagent (BD Biosciences) ...
-
bioRxiv - Plant Biology 2023Quote: ... Bait sequences (w/o signal peptides) were fused with the GAL4 transcription factor binding domain (GAL4-BD) in the p97-BD ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were then incubated in blocking buffer (PBS with 2% FBS) containing an anti-BrdU monoclonal antibody (BD Biosciences) (1:100 dilution ...
-
bioRxiv - Cell Biology 2022Quote: ... Inhibition of cell-cell junctions was achieved using a VE-cadherin function-blocking antibody (clone 75, BD Biosciences 610251) in which the cells were incubated at 12.5 μg/ml for 2 h before fixation.
-
bioRxiv - Immunology 2020Quote: ... or anti-CD206 blocking antibody (10 μg/ml clone 15.2 321102, BioLegend; isotype control mouse IgG1 kappa clone 107.3; 554721, BD Biosciences) were added to cultures 60 minutes before phagocytosis assays ...
-
bioRxiv - Cancer Biology 2021Quote: ... then Fc-receptors were blocked in Fc-blocking solution for 10 minutes at room temperature (BD, San Jose, CA). Sections were further blocked with with 3% goat serum in TBST for 30 minutes at room temperature ...
-
bioRxiv - Immunology 2021Quote: ... cells were incubated with a Fixable Viability Dye (eBioscience) and then with 2.4G2 antibody for FcγRII/III blocking (BD Pharmingen). Surface staining was performed at 4°C in staining buffer (PBS 1X-FCS 2%-EDTA 5mM) ...
-
bioRxiv - Cell Biology 2021Quote: ... Digested cells were incubated with blocking antibody Rat Anti-Mouse CD16/CD32 (BD Biosciences, Cat. No. 553142, 1:100) for 10 min ...
-
bioRxiv - Microbiology 2023Quote: ... BM cell suspensions (2×107/mL concentration) were treated with FcɣR-blocking agent (anti-CD16/32, clone 2.4G2, BD Biosciences) for 15 min ...
-
bioRxiv - Immunology 2023Quote: ... Non-specific antibody binding was blocked by incubating the cells with Fc-blocking antibody 2.4G2 (BD, 25 μg/ml) for 10 minutes on ice ...
-
bioRxiv - Immunology 2023Quote: Cells were kept at 4ºC while being stained in PBS supplemented with 0.5% BSA and 2mM EDTA in the presence of antibody blocking CD16/32 (clone 2.4G2; BD 553142). All antibodies were used at a 1:200 dilution vol/vol (v/v) ...
-
bioRxiv - Molecular Biology 2023Quote: ... and then sequentially incubated in blocking solution (PBS, 0,5% Tween 20,1% BSA) containing anti-BrdU primary antibody (1/100, BD Biosciences) and anti-mouse secondary antibody coupled to FITC (1/300 ...
-
bioRxiv - Neuroscience 2023Quote: ... Detection was accomplished by incubating with anti-ATXN2 primary antibodies (diluted 1:500 in blocking solution, BD Biosciences 611378) overnight at 4°C ...
-
bioRxiv - Immunology 2019Quote: ... The MHC monomers exchanged with peptides were tetramerized in the presence of allophycocyanin (APC) conjugated streptavidin (BD Biosciences) for 30 min at 37 °C ...
-
bioRxiv - Neuroscience 2021Quote: ... by subcutaneous injection of 200mg MOG peptide 35–55 in complete Freund’s adjuvant (Mycobacterium tuberculosis at 3.75 mg/ml; BD) followed by injection of 500ng of pertussis toxin (Sigma ...
-
bioRxiv - Immunology 2019Quote: ... SIVmac239 Gag Peptide Set #12364) in the presence of anti-CD28 (1 μg/mL, clone L293, BD Biosciences) and anti-CD49d (1 μg/mL ...
-
bioRxiv - Microbiology 2020Quote: ... N or M peptide pools (10μg/ml) in the presence of anti-CD28 (1μg/ml; BD Biosciences, USA) and recombinant interleukin (IL)-2 (20U/ml ...
-
bioRxiv - Immunology 2021Quote: Splenocytes were re-stimulated specifically for CD8+ T cells with 2 μg/mL B8R peptide (TSYKFESV, BD Pharmingen) with 5 μg/mL Brefeldin A (Invitrogen ...
-
bioRxiv - Cell Biology 2020Quote: ... resuspended in 100 μl PBS/5% FBS and blocked with 2.5 μg of Fc-blocking solution (BD Bioscience, Cat #564220) for 15 min at room temperature ...
-
bioRxiv - Neuroscience 2020Quote: ... Membranes were blocked with Odyssey blocking buffer and incubated with anti-LRP1 (R2629 or 5A6) or anti-SORL1 (BD Biosciences) at a concentration of 1:1000 overnight at 4 °C ...
-
bioRxiv - Cell Biology 2020Quote: ... IdU and CldU were then detected with the following primary antibodies in blocking solution for 1 hour at room temperature: mouse anti-BrdU (B44) (1:25) (BD) for IdU and rat anti-BrdU [Bu1/75 (ICR1)] (1:100 ...
-
bioRxiv - Cancer Biology 2021Quote: ... slides were incubated for 2.5 hr with primary antibodies diluted in blocking buffer (IdU, B44, 347580; BD; CldU, ab6326, Abcam), washed several times in PBS ...
-
Single cell transcriptomics reveals the effect of PD-L1/TGF-β blockade on the tumor microenvironmentbioRxiv - Genomics 2020Quote: ... The liquid was poured off from the cells (leaving approximately 100 µl in each tube) and 5 µl of mouse Fc Blocking Reagent (rat anti-Mouse CD16/CD32 BD) was added to samples and incubated for 5 minutes at 4°C ...
-
bioRxiv - Immunology 2021Quote: ... followed by FcR blockage with mouse serum (2%) and FcR blocking anti-mouse CD16/32 antibodies (clone 2.4G2, BD Biosciences). Next ...
-
bioRxiv - Immunology 2021Quote: ... Cells were then treated with dasatinib for 1 hr at 37°C prior to blocking with Fc-block (BD Bioscience), 5 % normal mouse serum ...
-
bioRxiv - Immunology 2022Quote: ... Fc blocking was performed with a combination of antibodies diluted in FACS buffer with Brilliant Violet Stain Buffer (BD Biosciences) for 30 minutes at 4°C in the dark ...
-
bioRxiv - Systems Biology 2023Quote: ... 4×106 cells per sample were incubated in blocking solution containing 5% fetal bovine serum and 2% FcBlock (BD Biosciences) in PBS ...
-
bioRxiv - Immunology 2023Quote: ... Fc receptor-blocking mAb against CD16/32 (93), and fluorochrome-conjugated streptavidin (BUV395, eFlour450, APC and PE-Cy7) were purchased from BD, eBioscience ...
-
bioRxiv - Immunology 2023Quote: ... The protein binding capacity of the well surface was saturated with 300 μL blocking solution (PBS supplemented with 2% skim milk powder (BD) and 0.1% Tween 20 ...
-
bioRxiv - Immunology 2023Quote: ... Surface antigens were stained for 30 min at 4°C with indicated antibodies (Table.1) after Fc blocking for 4°C 30 min with anti-mouse CD16/32 (BD) without wash ...
-
bioRxiv - Immunology 2024Quote: ... The membrane was blocked with Blocking Buffer (Odyssey) for 1 h at room temperature before incubation with mouse anti-AP2A1 (BD, clone 8 ...
-
bioRxiv - Immunology 2021Quote: ... cells were re-stimulated with peptides for 6 hours in the presence of Leuko Act Cktl with GolgiPlug (BD) plus 50 IU/mL IL-2 ...
-
bioRxiv - Immunology 2020Quote: Cells were stimulated for 12h with SARS-CoV-2 peptide pools (0.25 μg/ml) in the presence of αCD28/αCD49d co-stimulatory antibodies (BD FastImmune™ ...
-
bioRxiv - Immunology 2020Quote: PBMCs (1−2×106) were stimulated with epitope-specific peptides (5 μM) and anti-CD28 mAb (0.5 μg/mL, BD) and expanded for 14 days in complete RPMI culture medium containing rIL2 (20 IU/mL ...
-
bioRxiv - Developmental Biology 2021Quote: ... The primary antibodies for flow cytometry were: rat anti-C-peptide-Alexa Fluor 647 (1:200, 565831, BD Pharmigen) and mouse anti-Glucagon-BV421 (1:100 ...
-
bioRxiv - Immunology 2021Quote: ... cells were re-stimulated with peptides for 6 hours in the presence of GolgiPlug and GolgiStop (BD Cat#550583) plus 50 IU/mL IL-2 ...
-
bioRxiv - Immunology 2021Quote: ... Slides were incubated overnight in blocking solution with the following primary antibodies (1:100): rat anti-mouse SiglecF (E50-2440, 552126 BD Biosciences), Armenian hamster anti-mouse CD80 (16-10A1 ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Membranes were incubated with primary antibodies overnight at 4℃ after 1h blocking in 5% skimmed milk (Becton, Dickinson and Company, USA) at room temperature ...
-
bioRxiv - Cancer Biology 2021Quote: ... A primary antibody dilution was prepared in the blocking solution with the antibodies diluted 1:200 for HIF1α (BD Bioscience, 610958), 1:50 for phosphor-S6 (CST ...
-
bioRxiv - Cell Biology 2021Quote: ... washed twice and resuspended in 200 μL of blocking buffer in the presence of 7-AAD (BD Biosciences Cat#: 51-68981E) or DAPI (Invitrogen Cat# ...
-
bioRxiv - Developmental Biology 2020Quote: ... The cells were washed 2 times in FCM Blocking Buffer then analyzed on a BD Accuri C6 Flow Cytometer (BD Biosciences).
-
bioRxiv - Microbiology 2021Quote: Spleen and liver cells were incubated (20 min, 4°C) with anti-Fc-gamma blocking antibody (clone 2.4G2) (BD Biosciences, Belgium) and were subsequently stained with fluorescent conjugated antibodies (30 min ...
-
bioRxiv - Developmental Biology 2019Quote: ... the cells were resuspended with blocking buffer (5% BSA in DPBS) and stained with PE-Cy7 rat anti-CD31 (1:400, 561410, BD biosciences), APC mouse anti-EpCAM (1:400 ...
-
bioRxiv - Cell Biology 2019Quote: ... 1-2×106 cells were incubated for 15 min at +4°C with blocking buffer (1xPBS, 1% BSA, 2mM EDTA) containing 5mg/ml α-CD16/CD32 (2.4G2, BD Biosciences). For surface staining ...
-
bioRxiv - Immunology 2020Quote: ... Cells were stained with fluorescent-conjugated anti-mouse antibodies after blocking CD16/CD32 with Fc Block (BD Biosciences, San Jose, CA). For intracellular staining ...
-
bioRxiv - Cell Biology 2020Quote: ... anti-Synapsin (1:500) and anti-CD133 (1:500) were used after Fc blocking with CD16/32 antibody (553141; BD Pharmingen). Respective Secondary antibodies ...
-
bioRxiv - Immunology 2020Quote: Cells isolated from the organs indicated in figure 4 and figure S9 were incubated with an Fc blocking agent (BD Pharminogen) for 10min at 4°C ...