4 5 5 5 Tetrafluoro 4 trifluoromethoxy pentan 2 one citations for Becton, Dickinson and Company

Quote: ... Cells were pelleted at 2000 g for 5 min in 5 ml polystyrene round bottom tubes (BD Falcon 352052), and washed three times in 5 ml 0.2% BSA in PBS-G containing 6 mM glucose ...

The suprapyramidal and infrapyramidal blades of the dentate gyrus exhibit different GluN subunit content and dissimilar frequency-dependent synaptic plasticity in vivo

Christina Strauch, et al., bioRxiv - Neuroscience 2024

Quote: ... sections were incubated with primary antibody solutions for 5 days at 4°C: mouse monoclonal antiNMDAR1 (GluN1) (1:200; 556308, PharMingen, Becton, Dickinson and Company, Frankline Lakes, USA) or rabbit polyclonal antiNMDAa1 (GluN2A ...

Single-cell analysis of human lung epithelia reveals concomitant expression of the SARS-CoV-2 receptor ACE2 with multiple virus receptors and scavengers in alveolar type II cells

Guangchun Han, et al., bioRxiv - Genomics 2020

Quote: ... Cells from patients 2 through 5 were stained following washing with anti-EPCAM-PE (347198, BD Biosciences;1:50 dilution in ice-cold phosphate-buffered saline ...

Triple tandem trimer immunogens for HIV-1 and influenza nucleic acid-based vaccines

Iván del Moral-Sánchez, et al., bioRxiv - Microbiology 2023

Quote: ... 5% CO2 for 2 h prior to the addition of Brefeldin A and monensin (BD Biosciences). After being left in culture overnight ...

Comparative analysis of temperature preference behavior and effects of temperature on daily behavior in eleven Drosophila species

Fumihiro Ito, Takeshi Awasaki, bioRxiv - Evolutionary Biology 2022

Quote: ... virilis and 5mm diameter for others) filled with 5% sucrose (Wako) and 2% Bacto agar (BD) on one side ...

PYCR1-dependent proline synthesis in cancer-associated fibroblasts is required for the deposition of pro-tumorigenic extracellular matrix

Emily J Kay, et al., bioRxiv - Cancer Biology 2021

Quote: ... 1.5×106 pCAF2 and 5×105 MCF10DCIS.com in 200 µl 50% growth factor reduced phenol red free Matrigel (BD Biosciences) in PBS were injected subcutaneously into the flank of 8 week old female BALB/c nude mice (Charles River) ...

Maintenance DNA methylation is essential for regulatory T cell development and stability of suppressive function

Kathryn A. Helmin, et al., bioRxiv - Immunology 2020

Quote: ... single-cell suspensions of spleen and lung tissue and blood at a concentration of 5 x 106 cells/mL were treated with RPMI plus 5 µL/mL Leukocyte Activation Cocktail with GolgiPlug (BD) or RMPI only and incubated for 4 hours at 37 °C ...

Tools for Genetic Engineering and Gene Expression Control in Novosphingobium aromaticivorans and Rhodobacter sphaeroides

Ashley N. Hall, et al., bioRxiv - Microbiology 2023

Quote: ... coli strains were grown in LB (10 g tryptone, 5 g yeast extract, 5 g NaCl per liter; BD 240230) aerobically ...

TLK1-mediated RAD54 phosphorylation spatio-temporally regulates Homologous Recombination Repair

Ishita Ghosh, et al., bioRxiv - Molecular Biology 2022

Quote: ... Cells were fixed with Crystal violet solution (3gm in 5% Methanol: 5% Isopropanol) (BD BBL Gram Crystal Violet, Cat # 212525) and counted manually using ImageJ ...

Primary cilia shape postnatal astrocyte development through Sonic Hedgehog signaling

Rachel Bear, Steven A. Sloan, Tamara Caspary, bioRxiv - Neuroscience 2024

Quote: ... 3mg tamoxifen/ 40g mouse weight in 300ul corn oil was administered intraperitoneally at E15.5 and E19.5 to the pregnant dam using a 1ml syringe and a 25G 5/8-inch needle (BD Biosciences) followed by subcutaneous administration of 3mg tamoxifen/ 40g mouse weight prepared in 20ul of corn oil at P0 ...

STING suppresses cancer pain via immune and neuronal modulation

Kaiyuan Wang, et al., bioRxiv - Cancer Biology 2021

Quote: ... followed by incubation in blocking buffer (1% anti-mouse-CD16/CD32, 2.4 G2, 2% FBS, 5% NRS,1% triton x100 and 2% NMS in HBSS; BD Bioscience) for 1h at room temperature ...

Satellite glial GPR37L1 regulates maresin and potassium channel signaling for pain control

Sangsu Bang, et al., bioRxiv - Neuroscience 2023

Quote: ... All dissociated cells were blocked with Fc receptors staining buffer (1% anti-mouse CD16/CD32, 2.4 G2, 2% FBS, 5% NRS, and 2% NMS in HBSS; BD Bioscience) and then stained with a standard panel of antibodies ...

Tbet expression by Tregs is needed to protect against Th1-mediated immunopathology during Toxoplasma infection in mice

Jordan Warunek, et al., bioRxiv - Immunology 2021

Quote: ... anti-CD4-V450 (clone RM4-5, BD Pharmingen), anti-CD8b-Percp-Cy5.5 (clone H35-17.2 ...

Dynamically Linking Influenza Virus Infection Kinetics, Lung Injury, Inflammation, and Disease Severity

Margaret A. Myers, et al., bioRxiv - Systems Biology 2021

Quote: ... CD4 (PE-Cy5, clone RM4-5, BD Biosciences), CD8α (BV605 ...

Polygenic mutations model the pleiotropic disease of Fanconi Anemia

Karl-Heinz Tomaszowski, et al., bioRxiv - Cancer Biology 2020

Quote: ... anti-CD4 (PE, clone RM4-5, BD Pharmingen) and anti-CD8 (FITC ...

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