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Citations for Becton, Dickinson and Company :
1401 - 1450 of 2032 citations for Recombinant Human CD86 Fc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Antibodies used for cell lines were anti-human CD340 (HER2) (#562349, BD). All flow cytometry experiments were performed using either LSR Fortessa or ARIA Fusion (BD) ...
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bioRxiv - Cancer Biology 2023Quote: ... mouse anti-human CD15 conjugated to PE/Cyanine7 (clone HI98, BD, 560827), mouse anti-human CD33 conjugated to PE/Dazzle 594 (clone WM53 ...
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bioRxiv - Cell Biology 2023Quote: Human blood was collected into a 3.2% sodium citrate tube (BD Vacutainer) and centrifugated (1,200 x g for 10 minutes at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... mouse anti-human FOXP3 conjugated to BB700 (clone 236A/E7, BD, 566526), mouse anti-human CD69 conjugated to PE (clone FN50 ...
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bioRxiv - Immunology 2023Quote: ... TNF-α and interferon (IFN)-α were quantitatively determined by BD™ CBA Human Th1/Th2 Cytokine Kit II (BD Biosciences, San Jose, CA) as described previously[18] ...
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bioRxiv - Cell Biology 2023Quote: ... purified mouse anti-human CD3 (clone HIT3a, BD Biosciences, Franklin Lakes, NJ); goat F(ab’)2 anti-mouse IgG-PE (Southern Biotech ...
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bioRxiv - Cancer Biology 2023Quote: ... mouse anti-human CD25 conjugated to BV650 (clone M-A251, BD, 563719), mouse anti-human CD11b conjugated to BV395 (clone ICRF44 ...
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bioRxiv - Immunology 2024Quote: For degranulation assays mouse anti-human CD107a conjugated with PE (BD Biosciences) was added to the PBMCs incubated overnight in mediumPBMC1 after washing with DPBS ...
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bioRxiv - Bioengineering 2024Quote: ... PE-Vio 770-conjugated anti-human CD34 (130-124-456, BD Miltenyi) and FITC-conjugated anti-human CD36 (555454 ...
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bioRxiv - Bioengineering 2024Quote: ... and ITS + Premix (1%) and human EGF (20 ng/mL) from BD Biosciences ...
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bioRxiv - Cancer Biology 2024Quote: ... and ITS + Premix (1%) and human EGF (20 ng/mL) from BD Biosciences ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were stained with antibodies against human CD45 (clone HI30, BD Biosciences) and mouse CD45 (clone 2D1 ...
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bioRxiv - Microbiology 2021Quote: ... the cryosections were incubated for 1 h in the blocking solution (PBS containing 5% goat serum and 5 μg/mL mouse BD Fc Block™) and then labeled overnight at 4°C with the APC-conjugated rat anti-mouse CD45 antibody (1:100 dilution ...
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bioRxiv - Immunology 2020Quote: ... The cells were then washed and resuspended in PBS supplemented with 2% FCS for FACS analysis (FACSCanto II; BD Bioscience, San Jose, USA). FACS data analysis was performed using BD FACSDivaTM Software 8 (BD Biosciences ...
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bioRxiv - Cancer Biology 2020Quote: ... Unspecific binding was prevented using the ells were stained using the BD Pharmingen Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) (Clone 2.4G2 ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated in the FC wash buffer (2h at 4 °C) containing FITC-coupled anti-CD44 antibody (1 to 80 BD Pharmingen Cat.No. 555478) or its isotype control (FITC Mouse IgG2b ...
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bioRxiv - Immunology 2021Quote: We permeabilized formalin-fixed glomerular and tubulointerstitial single cells with 0.25% Tween-20 in PEB buffer at room temperature for 30 minutes and blocked cells with Fc Block (clone 2.4G2, BD Biosciences, San Jose, CA) diluted in 0.25% Tween-20 on ice for 10 minutes ...
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Multiplexed single-cell transcriptomic analysis of normal and impaired lung development in the mousebioRxiv - Cell Biology 2019Quote: ... Cells were incubated in the dark with 2 μl/1×106 cells of CD16/32 antibody (Fc block; BD Biosciences, Mississauga, ON, Canada) for 15 minutes at RT ...
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bioRxiv - Physiology 2021Quote: ... Following resuspension in PBS and passage through a 70μm strainer, cells were incubated in Fc block solution (Bio Rad, Cat# BUF041A) and PerCP-conjugated anti-CD11b antibody (BD Pharminogen, Cat# 550993). After PBS washes ...
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bioRxiv - Immunology 2021Quote: ... samples were stained with the relevant combination of surface and intracellular antibodies in combination with Fc blocking antibody CD16/CD32 (BD Pharmingen, Cat. #553141). BV421 anti-MHCII(IA/IE ...
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bioRxiv - Immunology 2021Quote: ... cell pellets were resuspended in x μL of unlabeled CD16/32 mAb Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™), BD Pharmingen 553141) ...
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bioRxiv - Immunology 2022Quote: ... blood cells were stained in 50 µl 2% FCS RPMI medium with antibodies against CD11C (APC, clone HC3, BD biosciences, dilution 1/100), CD11b (PercPCy5.5 or Pacific Blue ...
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bioRxiv - Physiology 2023Quote: ... then cells were washed with 1x PBS and resuspended in FACS buffer and non-specific binding to Fc receptors was blocked by incubation with anti-CD16/32 antibody (BD Pharmingen, Cat# 553141) and the FACS antibodies BV711 conjugated anti-CD11b and Pacific blue conjugated anti-CD45 antibodies (Cat# 101241 ...
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bioRxiv - Immunology 2023Quote: ... cells were washed 2x in FACS buffer (5% FBS, 2 mM EDTA in PBS) and resuspended with anti-CD16/CD32 Mouse BD FC Block (BD Biosciences, Cat. 553142) in FACS buffer at a concentration of 10 ug/ml for 15 min ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then incubated at 4°C for 15 min with Fc Block (Rat anti-mouse CD16 and CD32; BD Pharmingen – Cat.553141) and 30 min with the antibody mixes ...
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bioRxiv - Immunology 2023Quote: Cells were first labelled with Fc block for 10 minutes at 4°C and then the following antibodies added: CD45-PE (BD, 30-F.11), Siglec F-APC (BioLegend ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell suspensions were blocked for 5 min at 4 °C with rat anti-mouse CD16/CD32 (#553142, Mouse BD Fc Block, BD Biosciences) in FACS buffer ...
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Regulatory T cells control type 1-driven immunopathology restraining GM-CSF-producing helper T cellsbioRxiv - Immunology 2024Quote: ... cells were restimulated ex vivo for 4-5h at 37 °C in complete RPMI medium (RPMI supplemented with 2mM L-Glutamine and 10% fetal calf serum (FCS)) with GolgiPlug (BD, 1:1,000 dilution), phorbol 12-myristate 13-acetate (50ng/ml ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated for 30 min on ice with biotinylated and Alexa Fluor 647-conjugated recombinant soluble S and PD-1-BB515 (EH12.1, BD Horizon, 1:100) in 2% FBS and 2 mM EDTA in PBS (P2) ...
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bioRxiv - Bioengineering 2020Quote: ... we also preincubated 200 µL of 2 µmol recombinant B56 protein with 1 µmol mouse anti-B56 antibody (Clone 23/B56α BD biosciences, 610615) and 1 µmol goat anti-mouse IgG Alexa Fluor 647 (Thermo Fisher A-21235 ...
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bioRxiv - Plant Biology 2023Quote: ... Antibody dilutions were made in 5% (w/v) bovine serum albumin (α-GST, α-His, α-HA, α-biotin-HRP, α-MLO2) in TBST or 5% (w/v) milk (α-Gal4 BD, α-Gal4 AD ...
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bioRxiv - Developmental Biology 2021Quote: ... were plated in buffer (PBS with 0.5% bovine serum albumin and 1 mM EDTA) and non-specific Fc binding was blocked via incubation with mouse anti-rat CD32 (BD Biosciences #550271; 1:200) for 30 minutes on ice ...
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bioRxiv - Immunology 2020Quote: The following rat anti-mouse antibodies were used for leukocyte analysis within each tissue: purified CD16/CD32 Fc blocking antibody (clone 2.4G2; BD Pharmingen™, San Jose, CA), PerCP-Cy™5.5 Ly6G antibody (clone 1A8 ...
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bioRxiv - Cancer Biology 2022Quote: ... Single cell suspensions containing 3×106 cells/sample were stained using appropriate fluorescent antibody cocktails (Listed in Supplementary Table S1) after Fc blocking and analyzed using flow cytometry (BD FACS Celesta/Novocyte Advanteon). Cells were fixed and permeabilized using intracellular staining reagents (Intracellular Fixation & Permeabilization Buffer Set ...
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bioRxiv - Immunology 2022Quote: ... and F15 NOD Nfκb1 heterozygote were suspended in biotin-free RPMI containing 0.1% azide and 3% FCS and surface stained in 96-well plates with the 10–3.6 PE (anti-I-Ag7) (BD PharMingen, Franklin Lakes, NJ, USA), which is class II major histocompatibility complex (MHC ...
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bioRxiv - Cancer Biology 2023Quote: ... APC-eFluor780-conjugated cKit and BV605-conjugated Fc Gamma (CD16/CD32) for 30 minutes followed by fixation and permeabilization using BD Cytofix/Cytoperm (BD Biosciences, San Jose, California). Intracellular staining using FITC-conjugated KI-67 was performed overnight at 4 degrees ...
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bioRxiv - Immunology 2023Quote: ... Cells were then stained with monoclonal antibodies conjugated to fluorescent molecules as listed in Supplemental Table 5 after treatment with Fc Block (BD Biosciences, Franklin Lakes, NJ). Cells were analyzed on either a BD LSR-II or BD LSRFortessa X-20 flow cytometer running BD FACSDiva software or a Cytek Aurora spectral cytometer running SpectroFlo software and analyzed using FlowJo software (TreeStar).
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bioRxiv - Microbiology 2023Quote: ... spun for 10 minutes and then resuspended in 50 μL of a 1:200 dilution of Fc block (CD16/CD32, clone 2.4G2, BD Biosciences, cat. 553141, 0.5mg/mL) in FACS buffer (PBS ...
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bioRxiv - Cell Biology 2020Quote: ... Samples of human cell lines were then analyzed on a FACSCalibur (BD Biosciences) and primary murine samples were analyzed on a FACSCanto (BD Bioscience).
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bioRxiv - Immunology 2020Quote: ... PE mouse anti-human CD71 (1:20 dilution, Clone M-A712, BD Pharmingen) and APC mouse anti-human CD86 (1:20 dilution ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were incubated with PE-conjugated mouse anti-human CD44 or CD151 (BD) for 30 min at 4°C ...
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bioRxiv - Synthetic Biology 2022Quote: ... PE-CF594 Mouse anti-human Akt pS473 clone M89-61 (BD Biosciences #562465), AF647 Mouse anti-human Akt pS473 clone M89-61 (BD Biosciences #560343).
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bioRxiv - Immunology 2022Quote: Healthy human whole blood was collected by venous puncture into collection tubes (BD catalog# 364606 ...
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bioRxiv - Immunology 2022Quote: ... Intracellular cytokine staining was performed with anti-human IFNγ and TNFα (BD Biosciences) antibodies for 30 min at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD45 PE-CF594 (1:100, clone HI30, BD Biosciences, cat # 562279), Anti-human CD29 PE Cy7 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD45RO PE Cy7 (1:50, clone UCHL1, BD Biosciences, cat # 560608), Anti-human MerTK PE Cy7 (1:50 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human RORgT BV650 (1:50, clone Q21-559, BD Biosciences, cat # 563424), Anti-human Perforin eF450 (1:100 ...
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bioRxiv - Genomics 2020Quote: ... PE-conjugated mouse anti-human CD31 antibody (1:25; clone WM59, BD Pharmingen), PerCP-conjugated streptavidin (1:400 ...
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bioRxiv - Immunology 2021Quote: ... followed by incubation with mouse anti-human CD31 (1:20, BD, Catalog 550389), and goat anti-human IFNL (1:25 ...
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bioRxiv - Immunology 2019Quote: ... followed by intracellular cytokine staining with anti-human IL-10-APC (BD Biosciences), IL-4-PERCP Cy5.5 (Biolegend ...