Human MXRA8 Mouse Fc Tag citations for Becton, Dickinson and Company

Quote: RFP+GFP+ FOXP3+ Tregs and RFP+GFP- exFOXP3 T cells isolated from spleen and CNS from EAE mice were stained with sample tag antibodies (Ms Single Cell Sampling Multiplexing Kit, BD Biosciences). Next ...

Polymorphic KIR3DL3 expression modulates tissue-resident and innate-like T cells

William H. Palmer, et al., bioRxiv - Immunology 2022

Quote: ... Cell subsets for the populations from different donors were labelled simultaneously with the cell surface sorting panel (Table S4) an oligonucleotide-tagged antibody sample tag (BD Biosciences) prior to cell sorting on a FACSAria3 (BD Biosciences ...

MAIT cells drive chronic inflammation in a genetically diverse murine model of spontaneous colitis

Liyen Loh, et al., bioRxiv - Immunology 2023

Quote: ... up to 2 x106 MR1-5-OP-RU-APC Tetramer magnetic bead enriched spleen (n= 2) or unenriched colon (n= 2) single cell suspensions were labeled with an oligonucleotide-tagged antibody sample tag (BD Biosciences). MAIT cells (MR1-5-OP-RU-Tet+TCRβ+CD3+ ...

LAG-3 blockade reactivates the CD8⁺ T cell expansion program to re-expand contracted clones in the tumor

Munetomo Takahashi, et al., bioRxiv - Immunology 2024

Quote: ... cells were stained with antibodies and anti-CD45 Sample Tag oligonucleotide-conjugated antibodies from the Single-Cell Multiplexing Kit (BD Biosciences). Before sorting ...

Human myogenic progenitor cells display tenogenic differentiation potential and facilitate tendon regeneration

Xiexiang Shao, et al., bioRxiv - Cell Biology 2024

Quote: ... The single cell suspension obtained from human muscle was stained with a cocktail containing PE-Cy5 anti-human CD45 (BD Pharmingen, cat#555484), Percp-Cy5.5 anti-human CD31 (BioLegend ...

Sphingosine-1-phosphate signaling inhibition suppresses Th1-like Treg generation by reversing mitochondrial uncoupling

Rachel Coulombeau, et al., bioRxiv - Immunology 2024

Quote: ... The antibodies used for stimulation were anti-human CD3 (clone UCHT1, plate bound) and anti-human CD28 (clone 28.2, BD Biosciences, San Jose, CA) at 1 μg/mL ...

A recombinant SARS-CoV-2 RBD antigen expressed in insect cells elicits immunogenicity and confirms safety in animal models

Ricardo Choque-Guevara, et al., bioRxiv - Immunology 2021

Quote: ... and then labeled with conjugated antibodies to surface antigens (PerCP-Cy®5.5 anti-mouse CD3, FITC anti-mouse CD4, APC-Cy®7 mouse anti-CD8, all from BD Biosciences ...

A recombinant SARS-CoV-2 RBD antigen expressed in insect cells elicits immunogenicity and confirms safety in animal models

Ricardo Choque-Guevara, et al., bioRxiv - Immunology 2021

Quote: Mononuclear cells were directly labeled with conjugated antibodies to surface antigens (PerCP-Cy®5.5 anti-mouse CD3, clone, FITC anti-mouse CD4, APC-Cy®7 anti-mouse CD8, for T lymphocyte phenotype, all from BD Biosciences ...

Prostaglandin E₂ promotes intestinal inflammation via inhibiting microbiota-dependent regulatory T cells

Siobhan Crittenden, et al., bioRxiv - Immunology 2020

Quote: ... for 2 h or overnight followed by staining with anti-mouse Foxp3 (clone FJK-16s), anti-Mouse ROR-γt (clone B2D, eBioscience) and mouse anti-Stat1 (pY701) (clone 4a, BD UK) for at least 1 hour ...

A high-throughput CRISPR interference screen for dissecting functional regulators of GPCR/cAMP signaling

Khairunnisa Mentari Semesta, et al., bioRxiv - Cell Biology 2020

Quote: ... resuspended in 100 μl PBS/5% FBS and blocked with 2.5 μg of Fc-blocking solution (BD Bioscience, Cat #564220) for 15 min at room temperature ...

Viral infection engenders bona fide and bystander lung memory B cell subsets through permissive selection

Claude Gregoire, et al., bioRxiv - Immunology 2021

Quote: ... cells were either resuspended in 400μl of PBS 2%FCS 2mM EDTA and analyzed on Fortessa-X20/Symphony cytometers (BD Biosciences) or in RPMI media and used for cell sorting on a FACSAria II (BD ...

Prenatal immune stress induces a prolonged blunting of microglia activation that impacts striatal connectivity

Lindsay N. Hayes, et al., bioRxiv - Neuroscience 2021

Quote: ... The cells were incubated with Fc block (eBiosciences 16-0161-81) for 5 min then incubated with antibodies (CD45-APC BD Biosciences 561018 ...

Prenatal immune stress induces a prolonged blunting of microglia activation that impacts striatal connectivity

Lindsay N. Hayes, et al., bioRxiv - Neuroscience 2021

Quote: ... The cells were incubated with Fc block (eBiosciences 16-0161-81) for 5 min then incubated with antibodies (CD45-APC BD Biosciences 559864 ...

Enhancing mitochondrial activity in neurons protects against neurodegeneration in CNS inflammation

Sina C. Rosenkranz, et al., bioRxiv - Neuroscience 2020

Quote: ... and microglia (CD45med CD11b+were sorted into collection tubes coated with FCS and filled with complete RPMI 1640 medium (1% penicillin, streptomycin, 0.1% 2-ME) with 20% FCS using a FACSAria device (BD Biosciences). Purity of sorted populations was routinely above 95% ...

Metabolic dysfunction induced by high-fat diet modulates hematopoietic stem and myeloid progenitor cells in brown adipose tissue of mice

Kyle T Mincham, et al., bioRxiv - Cell Biology 2021

Quote: ... Single cell suspensions for myeloid panel staining were incubated in Fc Block (Cat#553142, BD Biosciences, Franklin Lakes, NJ, USA). Fluorescent minus one staining controls were used for both panels where required ...

Plasmacytoid dendritic cells regulate megakaryocyte and platelet homeostasis

Florian Gaertner, et al., bioRxiv - Immunology 2022

Quote: For RNAseq analysis BM cells were isolated by flushing the long bones with FACS buffer (2mM EDTA, 1% FCS, PBS) and treated with Pharm LyseTM buffer (BD). Cells were enriched by magnetic removal of CD11b+ and CD19+ cells (EasySepTM ...

Nuclear receptor corepressor 1 controls regulatory T cell subset differentiation and effector function

Valentina Stolz, et al., bioRxiv - Immunology 2022

Quote: Ex vivo isolated T cells and in vitro-cultured T cells were incubated with Fc-block (1:250; BD Biosciences) followed by surface staining ...

A flexible, efficient, and scalable platform to produce circular RNAs as new therapeutics

Chuyun Chen, et al., bioRxiv - Bioengineering 2022

Quote: ... Cells were then washed with FC buffer (PBS supplemented with 2% HI-FBS and 0.05% NaN3) and resuspended in Fixation Buffer (BD Pharmingen)for 5 min at 4C° in the dark ...

Role of TLR4 in persistent Leptospira interrogans infection: a comparative in vivo study in mice

Nisha Nair, et al., bioRxiv - Microbiology 2020

Quote: ... and 3×106 cells per tube were stained as follows: cells were incubated in 0.5 μg Fc block (BD Biosciences) for 15 min at 4°C in staining buffer (Ca2+ - and Mg2+ - free PBS containing 3% heat-inactivated fetal bovine serum ...

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