Labshake search
Citations for Becton, Dickinson and Company :
1401 - 1450 of 1589 citations for 6 Nitro 2 Methylbenzothiazole since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2023Quote: ... cells were fixed for a minimum of 20 minutes with 2% paraformaldehyde and acquired immediately using a FACS Symphony A3 (BD Biosciences). Where intracellular staining was performed ...
-
bioRxiv - Immunology 2023Quote: ... Cells were sorted by their distinct proliferation peaks (peak 0, peak 1 and peak 2) on FACS-Aria and Influx Cell Sorters (BD Biosciences). Cells were kept on ice and after washing with cold PBS were immediately resuspended in Trizol for RNA extraction ...
-
bioRxiv - Immunology 2022Quote: FACS-sorted Tregs were plated at 50,000 cells/well in round bottom 96 well plates pre-coated with anti-human CD3 (2 μg/ml, clone UCHT1, BD Biosciences) and soluble anti-human CD28 (1 μg/ml ...
-
bioRxiv - Pathology 2023Quote: ... Placentas were perfused for 3 hours and 1 ml (T4 transfer experiments) or 2 ml (TRIAC transfer experiments) samples were collected from the reservoirs into blood collection tubes (BD vacutainer) at the time points indicated in the figures ...
-
bioRxiv - Immunology 2022Quote: ... The cells were fixed in 2% paraformaldehyde and GFP expression measured and quantitated using a FACS Canto II flow cytometer and FlowJo software (BD Biosciences). IC50 values were calculated using Prism software (GraphPad/Dotmatics).
-
bioRxiv - Developmental Biology 2023Quote: ... GFP and mCherry+ cells were collected in 50 µl 2% BSA in PBS via fluorescence-activated cell sorting (BD LSR Fortessa) and processed for single-cell RNA sequencing analysis based on 10X Genomics (10X Chromium system ...
-
bioRxiv - Immunology 2023Quote: ... followed by staining with a cocktail of mAbs and four fluorochrome-conjugated spike proteins in staining buffer (2% FBS/PBS) supplemented with Brilliant Stain Buffer Plus (BD Biosciences). The stained cells were fixed (Lysing Solution ...
-
bioRxiv - Immunology 2023Quote: ... plates were incubated with serum (2 fold dilutions, 1:20-1:2560) and parasite specific antibody was measured using biotinylated IgG1 (BD Biosciences) or IgG2c (BD Biosciences ...
-
bioRxiv - Cancer Biology 2023Quote: ... were stained with the specified antibodies on ice for 30 min in D-PBS supplemented with 2% FBS in the dark and analyzed using a Fortessa or Symphony A3 (BD Biosciences). Data was analyzed using FlowJo10 (Tree Star Inc. ...
-
bioRxiv - Developmental Biology 2023Quote: ... resuspended in 300 μl of 2% FBS in PBS with 1000 ng/μl DAPI and processed by FACS on a Melody Cell Sorter (BD Biosciences) using the BD FACSChorus software ...
-
bioRxiv - Cancer Biology 2023Quote: ... cells were washed with PBS three times and resuspended in FACS buffer (PBS with 2%FBS) for analysis on BD Biosciences LSR Fortessa using a 488-nm laser and 530-nm/30 filter (BD Biosciences). Data analysis was performed with FCS Express 7 (De Novo Software) ...
-
bioRxiv - Bioengineering 2023Quote: ... TCR expressing cells were mixed with K562-A2 cells at a ratio of 1:2 (Effector:Target) in the RPMI media and supplemented with 1 µg/ml of anti-CD28/CD49d antibodies (BD Biosciences, 347690) and 1 µg/ml of cognate peptides ...
-
bioRxiv - Biophysics 2022Quote: ... Saccharomyces cerevisiae strain W303-GAL4 was grown overnight in YPDA medium (yeast extract 1 %, adenine sulfate 540 μM, peptone 2 % (BD Biosciences), glucose 2 % ...
-
bioRxiv - Cancer Biology 2023Quote: C4-2 cells were cultured and lifted from plates by trypsinization then re-suspended in 100 mL of 50% Matrigel (BD Biosciences) and saline mixture followed by subcutaneous injection in two separate groups of castrated and non-castrated athymic nude mice (age at least 6 weeks old) ...
-
bioRxiv - Cancer Biology 2023Quote: Single cell suspensions were pre-incubated for 15 minutes in a staining solution (PBS+/+, 2% FBS) containing 1 μg/mL Fc block (BD Biosciences), followed by an incubation with the antibodies for 45 minutes (Table S1) ...
-
bioRxiv - Microbiology 2023Quote: ... PBMCs were then stained with live/dead and cell surface markers in MACS solution (Miltenyi) supplemented with 2% BSA (Miltenyi) prior to permeabilization with Cytofix/Cytoperm (BD Biosciences) and staining with intracellular markers in Perm/Wash (BD Biosciences) ...
-
bioRxiv - Microbiology 2023Quote: ... PBMCs were then fixed in 2% formaldehyde and stored at 4°C until flow cytometry on an LSR II flow cytometer (BD Biosciences). Cell surface markers included CD3 (clone 17A2 ...
-
bioRxiv - Immunology 2022Quote: ... cells were fixed with 2% paraformaldehyde for 20 min and resuspended in 350 ul 1% BSA/PBS for phenotyping by FACSDivacytometer (BD Biosciences). To set up the compensation control ...
-
bioRxiv - Immunology 2023Quote: ... T cells isolated from lymph nodes or tumors were mixed with 3×106 congenically marked B6 splenocytes and incubated with 1 μg/mL of OVA peptide and/or 2 μg/mL of GP peptide for 4-5h at 37°C in the presence of GolgiPlug (BD Biosciences). After staining for cell surface molecules ...
-
bioRxiv - Immunology 2023Quote: ... Antibody cocktails for surface proteins were prepared in PBS + 2% FBS and Brilliant Stain Buffer (50 µl/100 µl of cocktail, BD Biosciences) and added to the cells ...
-
bioRxiv - Molecular Biology 2023Quote: ... Anaerobic bacteria colonies were counted after incubating these agar plates for 2 days using the GasPak anaerobic gas generating pouch system (BD Biosciences).
-
bioRxiv - Microbiology 2023Quote: ... GYM (glucose 4 g, yeast extract 4 g, malt extract 10 g, CaCO3 2 g, and Bacto Agar (BD Difco™) 20 g ...
-
bioRxiv - Animal Behavior and Cognition 2023Quote: ... and the second model investigated ii) four exposures from the first eating event (AK-20, BD, KB, LT & NH; Model 2). In each model ...
-
bioRxiv - Immunology 2023Quote: ... 1 μg/ml each of ESAT-6 and CFP-10 peptide pools (provided by Aeras, Rockville, MD) or R10 media for 2 h before adding 10 μg/ml BD GolgiPlug (BD Biosciences) for a further 12 hours ...
-
bioRxiv - Immunology 2023Quote: ... prior to stimulation for 5 h with anti-CD3 and anti-CD28 without IL-2 in the presence of brefeldin A and monensin (BD Biosciences).
-
bioRxiv - Immunology 2023Quote: ... The tube was then stored in the dark at 4°C for up to 2 hours prior to acquisition on a LSRII or FACSLyric analyzer (BD Biosciences).
-
bioRxiv - Immunology 2023Quote: ... solution for 20 minutes followed by 20 minutes of antibody cross linking on ice with 2 µg/mL goat-anti mouse solution (BD, #53998). T cells were then activated via an incubation period at 37 °C in FACS buffer (PBS with 2% FBS and 2 mM EDTA ...
-
bioRxiv - Cancer Biology 2023Quote: ... or FAK-KD cell lines were generated by injecting a total of 2×106 MDA-MB-231/Dendra2 cells resuspended in 20% collagen I (BD Biosciences) in PBS into the lower left mammary gland of SCID mice ...
-
bioRxiv - Cell Biology 2023Quote: ... Cell pellets were dissolved in 2% FBS in PBS and flow cytometry was performed using an Accuri C6 Plus (BD Biosciences) or Citole Flow Cytometer (Beckman Coulter).
-
bioRxiv - Genomics 2023Quote: ... at least 2 × 106 cells were fixed and permeabilized using the Fixation/Permeabilized Solution Kit with BD GolgiStop TM (BD Biosciences) following the manufacturer’s recommendations ...
-
bioRxiv - Immunology 2023Quote: ... The proteases were inactivated by adding 10% fetal bovine serum and 2 mM EDTA and the cell disaggregated by passing them through a 40 μm nylon sieve (BD Bioscience). Single cells were then washed with 1% Bovine serum albumin (BSA ...
-
bioRxiv - Immunology 2023Quote: ... Samples for flow cytometry were fixed in 2% paraformaldehyde solution in PBS and analyzed using a LSRII flow cytometer (BD Biosciences) and FlowJo software (Tree Star ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were suspended in 350 µL of FACS buffer (1XDPBS, 2% FBS, 25mM HEPES, 2mM EDTA) and was analysed by flow cytometry BD LSRFortessa (BD Biosciences). 10,000 cells were analysed for each condition ...
-
bioRxiv - Developmental Biology 2024Quote: ... 1 – 2 mL of thin albumin was aspirated out with a 5 mL syringe (Nipro) and a 18G sterile needle (BD PrecisionGlide) from the blunt end of the egg to create a space between the embryo and the shell ...
-
bioRxiv - Bioengineering 2024Quote: ... for viability assessment and fixed with 2% paraformaldehyde (30 min incubation) until analysis by flow cytometry using an LSR Fortessa (BD Biosciences). For assessment of NP-associated fluorescence in a fluorescence plate reader ...
-
bioRxiv - Immunology 2024Quote: ... Cell culture supernatants were assayed for IL-2 using capture and biotin-labeled detection antibody pairs (BD Biosciences, Franklin Lakes, NJ). Detection was performed with HRP-conjugated streptavidin (BD Biosciences ...
-
bioRxiv - Immunology 2024Quote: ... the cells were fixed with 2% (w/v) paraformaldehyde and stored at 4°C before analysis with FACS Celesta Cell Analyzer (BD Biosciences). Data were analyzed using FlowJo software version X.0.7 (Tree Star ...
-
bioRxiv - Cell Biology 2024Quote: ... was added to a final concentration of 2 μg/mL and cells examined by flow cytometry using a FACSLyric flow cytometer (BD Biosciences). For controls (negative ...
-
bioRxiv - Immunology 2024Quote: T cells were restimulated with 3 μg/ml plate-bound anti-CD3 and 2 μg/ml soluble anti-CD28 or PMA/Ionomycin in the presence of GolgiPlug protein transport inhibitor (BD Biosciences) for 6 hours ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... Isolates from the CNRMA in France were subcultured on 2% malt extract agar (Oxoid) and potato dextrose agar (BD Diagnostic Systems) for 5 days at 30 °C ...
-
bioRxiv - Cancer Biology 2021Quote: ... The bottom layer of soft agar was prepared by mixing 2 × growth medium and 1.5% noble agar (BD Biosciences, San Jose, CA) at a 1:1 ratio and the mixture was poured into 6-well plates ...
-
bioRxiv - Cell Biology 2020Quote: ... resuspended in buffer solution (PBS + 2% FBS + 2mM EDTA) and blocked with recombinant Fc protein (1:100 dilution, BD Biosciences, cat#553142). Cells were then incubated with the primary antibody solution (1:100 dilution ...
-
Planarian CREB-binding protein (CBP) gene family regulates stem cell maintenance and differentiationbioRxiv - Developmental Biology 2020Quote: ... which was incubated for 2 h in the dark at 37°C with 20 µM caspase-3 substrate Ac-DEVD-AMC (BD Biosciences Pharmingen) or 2 ml from a stock of 1 mg/ml for a final volume of 150 µl ...
-
bioRxiv - Neuroscience 2021Quote: ... Primary antibodies used for immunofluorescence of HeLa cells and/or primary neurons were: mouse anti-Ataxin-2 (BD Biosciences 611378, 1:250), mouse anti-beta-III-tubulin (Covance MMS-435P ...
-
bioRxiv - Developmental Biology 2019Quote: ... Passages were performed by resuspending Matrigel-embedded organoids in cold DPBS and transferring them to a Falcon tube using a 2 mL syringe with a 27 G ½ needle (BD Microlance #300635) to break them up ...
-
bioRxiv - Cancer Biology 2020Quote: ... cells were washed with 1x Permeabilization buffer (eBioscience) and incubated for 15 min in FACS buffer (PBS, 2% FCS) before adding the primary MASH-1 antibody (BD Biosciences, 556604), 1:250 ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... 4 μL of the saturated culture were transferred in 60 uL PBS and the samples were stored at 4 °C for 1-2 days before being assayed by flow cytometry (BD FACSCanto II). Data were analyzed in FlowJo ...
-
bioRxiv - Bioengineering 2020Quote: ... we also preincubated 200 µL of 2 µmol recombinant B56 protein with 1 µmol mouse anti-B56 antibody (Clone 23/B56α BD biosciences, 610615) and 1 µmol goat anti-mouse IgG Alexa Fluor 647 (Thermo Fisher A-21235 ...
-
bioRxiv - Bioengineering 2021Quote: ... and stained for 1 hour at 4 °C with 2 µl of the following Abs: PE-Cy7 conjugated anti-mouse IFN-γ (BD Biosciences), PE conjugated anti-mouse IL-2 (Invitrogen eBioscience) ...
-
bioRxiv - Bioengineering 2021Quote: ... albicans fungal cells (CMM16) were inoculated from a streaked plate in 2 ml yeast extract peptone dextrose (YPD) [1% yeast extract (BD Biosciences, 212730), 2% peptone (BD Biosciences ...