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Citations for Becton, Dickinson and Company :
1351 - 1400 of 6989 citations for 8 CYANO IMIDAZO 1 2 A PYRIDINE 2 CARBOXYLIC ACID ETHYL ESTER since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: Cryopreserved cells were thawed at room temperature and washed twice with 10 ml of FACS buffer (PBS and 2% FBS) and filtered through a 100 µM filter (BD BioSciences, cat no: 352360). Cell concentration was determined by hemocytometer ...
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bioRxiv - Immunology 2021Quote: ... the cells were fixed with 2% formaldehyde solution (Tousimis, Rockville MD) and fluorescence was evaluated on an LSRII flow cytometer (BD Bioscience, San Jose, CA). The phagocytic score was calculated by multiplying the percentage of bead-positive cells by the geometric mean of the fluorescence intensity of bead-positive cells and dividing by 10,000.
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bioRxiv - Immunology 2022Quote: ... the cells were fixed with 2% PFA and stored at 4°C until the samples were acquired on a LSRII cytometer (BD Biosciences, Mississauga, ON, Canada). Data analysis was performed using FlowJo v10.5.3 (Tree Star ...
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bioRxiv - Biochemistry 2022Quote: ... either single GFP+ cells (to obtain clones 1 and 2) or 250 GFP+ cells (to obtain the pool) were sorted per well into a 96 well plate (BD FACSAria Fusion Cell Sorter). The two clones and the pool were expanded and the decreased ArgBP2 expression was confirmed by immunoblotting.
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bioRxiv - Immunology 2019Quote: ... The cells were then washed twice in media and resuspended in 2% paraformaldehyde until they were run on a BD LSRII Flow Cytometer (BD BioSciences, San Jose, CA). Flow data were analyzed using Flowjo version 10.5.3.
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bioRxiv - Immunology 2021Quote: ... PBMCs were unstimulated (DMSO) or stimulated with SARS-CoV-2 spike (S) peptide pools (5 mg/mL) in presence of GolgiPlug (BD Biosciences, San Jose, CA) for 6 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were fixed using 2% paraformaldehyde (PFA) solution for 30 minutes and permeabilization was performed using a 1X BD perm/wash solution (BD Biosciences, USA; cat#554723) for 15 minutes at 4°C ...
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bioRxiv - Immunology 2020Quote: ... the cells were co-cultured in R10 medium alone or in presence of K562 cells for 2 hours in the presence of anti-human CD107a (FITC or Brilliant Violet 421, H4A3, BD Biosciences, San Jose, Calif.).
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bioRxiv - Immunology 2021Quote: ... The following antibodies were used in the cocktail for surface staining: CD3-allophycocyanin (APC)-Cy7 (clone SP34-2, Cat#557757, BD Pharmingen, San Jose, CA), CD4-PE-CF594 (clone L200 ...
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bioRxiv - Immunology 2021Quote: ... cryopreserved PBMCs were assessed to determine the frequency of circulating T follicular helper (Tfh) using a panel which included the following antibodies: CD3 (BD Biosciences; clone SP34-2), CD4 (BD Biosciences ...
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bioRxiv - Microbiology 2021Quote: All mycobacterial strains (Supplementary File 2 – Key Reagents) were grown in liquid culture containing Difco™ Middlebrook 7H9 Broth (BD Biosciences, San Jose, CA) and supplemented with 0.2 % (v/v ...
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bioRxiv - Immunology 2020Quote: ... Cells were collected from wells by pipetting with PBS (containing 2%FBS and 2mM EDTA) and data acquisition was performed on a BD Accuri C6 (Becton, Dickinson and Company, USA). All flow cytometry data were analyzed with FlowJo v10.1 (BD Biosciences).
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bioRxiv - Microbiology 2022Quote: ... After 19h incubation at 37°C, the cells were fixed with 2% formaldehyde solution (Tousimis, Rockville, MD) and fluorescence was evaluated on a LSRII (BD Bioscience, San Jose, CA). The phagocytic score was calculated by multiplying the percentage of bead-positive cells by the geometric mean fluorescence intensity (MFI ...
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bioRxiv - Bioengineering 2023Quote: ... Fixed and permeabilized cells were washed twice with PBSA buffer and incubated for 2 hr at room temperature with Alexa Fluor 647-conjugated anti-STAT5 pY694 antibody (BD Biosciences, clone 47/Stat5) diluted 1:50 in PBSA buffer ...
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bioRxiv - Immunology 2024Quote: ... blocked with 2% BSA in phosphate buffer pH=7.4 for 30min at RT and incubated with mouse anti-human LAMP1 (BD Biosciences, Franklin Lakes, NJ, USA) for 1h at RT ...
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bioRxiv - Microbiology 2024Quote: ... Cells were enumerated using a Cellometer X2 cell counter (Nexcelom Bioscience, Lawrence, MA, USA) and resuspended in yeast nitrogen base supplemented with 2% glucose (Difco; BD, Franklin Lakes, NJ, USA), to obtain a concentration of 2.0 × 106 cells/ml ...
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bioRxiv - Microbiology 2024Quote: ... Bacterial strains were prepared for agar overlay plating by streaking out from glycerol stocks onto 2% streak agar plates (BD Difco 2216 marine broth), and allowed to grow for one days at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... and Colistin Naladixic Acid (CNA) agars (BD BBL™ Prepared Plated Media) and incubated in 5% CO2 at 35°C for 48 hours ...
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bioRxiv - Microbiology 2022Quote: ... CAA for 1l: 5g of Bacto Casamino Acids Technical (BD ref 223120), 0.25g MgSO4 · 7 · H2O (Sigma CAS 10034-99-8) ...
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bioRxiv - Bioengineering 2022Quote: ... supplemented with OADC (Oleic acid, Albumin, Dextrose, Catalase) enrichment medium (BD Biosciences). Bacterial cultures were harvested ...
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bioRxiv - Biochemistry 2020Quote: ... 6.7 g/l yeast nitrogen base w/o amino acids (BD Biosciences), 2% glucose ...
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bioRxiv - Cancer Biology 2022Quote: ... Matching whole blood samples were collected in acid citrate dextrose tubes (BD) and processed within 3-4 hours of phlebotomy (n=10 ...
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bioRxiv - Microbiology 2022Quote: ... 10% oleic acid-albumin-dextrose catalase (OADC) (BD Difco, Frankin Lakes, USA), 0.05% (vol/vol ...
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bioRxiv - Microbiology 2023Quote: ... or selective Ca-medium: 5 g/L BactoTM casamino acids (BD bioscience); pH adjusted to 7.2 with KOH (30) ...
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bioRxiv - Biochemistry 2023Quote: ... 6.7 g/l yeast nitrogen base w/o amino acids (BD Biosciences), 2% glucose ...
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bioRxiv - Microbiology 2021Quote: ... Slides were incubated with an anti-IL-8 antibody (Cat. # 550419, BD Pharmingen™) in 5% NGS with PBS at 4°C for overnight ...
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bioRxiv - Biochemistry 2019Quote: ... and NPC2 mutant fibroblasts were plated onto 8-well tissue culture slides (BD falcon) at a density of approximately 20,000 cells per well and incubated at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... We index sorted 8 96-well plates using a FACS Aria II (BD Biosciences) interfaced with FacsDiva software (BD Biosciences) ...
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bioRxiv - Cancer Biology 2022Quote: Migration assay were conducted with 8-μm pore PET transwell inserts (BD Falcon™) and TC-treated multi-well cell culture plate (BD Falcon™) ...
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bioRxiv - Immunology 2019Quote: ... IL-8 and Cxcl15 were analyzed by ELISA kits following the manufacturer’s instructions (BD Bioscience and R&D Systems ...
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bioRxiv - Immunology 2019Quote: ... 8-color flow cytometer with BD Diva Software (BD Bioscience San Jose, CA, USA) and analyzed using FlowJo version 9.7.7 (Tree Star Ashland ...
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bioRxiv - Immunology 2020Quote: ... using DIVA 8 acquisition software and a FACS Aria III cell sorter (BD Biosciences), at single cell density into hard shell 96-well PCR plates (BIORAD ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated at 37°C for 8 hr with Brefeldin A (BD Biosciences) and Monensin (ThermoFisher ...
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bioRxiv - Cell Biology 2022Quote: ... containing inactivated Mycobacterium tuberculosis H37Ra (6 mg/mL, BD B231141, Cat. DF3114-33-8). Mice were also co-injected with pertussis toxin (200 ng/mouse in 1X PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... with an 8-μm pore size were pre-coated with Matrigel (BD Biosciences, 354234) [30] ...
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bioRxiv - Immunology 2019Quote: ... Serum was collected by tail-tipping before LPS injection and 2 and 4 hours following injection for IL6 determination by ELISA (BD; OptEIATM Set Mouse IL-6), as per manufactures instructions.
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bioRxiv - Immunology 2021Quote: ... cells were washed and resuspended in PBS/2% FBS for analysis on a five-laser flow cytometer (FACSymphony A3 Cell Analyser, BD Bioscience, San Jose, CA, USA). Data were analyzed with FlowJo software (TreeStar ...
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bioRxiv - Neuroscience 2020Quote: Neurons from 18-day-old rat embryos were dissected and dissociated using trypsin and plated at a density of 70,000 cells cm−2 in minimal essential medium (MEM) supplemented with 10% NU serum (BD Biosciences, Le Pont de Claix, France), 0.45% glucose ...
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bioRxiv - Immunology 2021Quote: ... and intracellular cytokines (PE anti-mouse IFN-γ, PE-Cy®7 anti-mouse TNF-α, APC anti-mouse IL-2, all from BD Biosciences, USA). The labeled cells were acquired with the BD FACSCanto™ II flow cytometer and analyzed with the program FlowJo v.10.6.2 (BD Biosciences).
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... we allowed organotypic cultures to differentiate at an air-liquid interface (ALI) in a defined differentiation media [base media with 2% v/v NuSerum (BD BioSciences, San Jose, CA, USA), and 50 nM retinoic acid (Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... we allowed organotypic cultures to differentiate at an ALI in a defined differentiation media supplemented with IL-13 [base media with 2% v/v NuSerum (BD BioSciences, San Jose, CA, USA), 50 nM retinoic acid (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: The actinobacteria were routinely cultured from frozen spore preparations via inoculation into Difco International Streptomyces Project (ISP) medium 2 (ISP2) (Becton, Dickinson and Company, Spark, MD) and incubated at 28° C ...
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bioRxiv - Immunology 2020Quote: ... before being washed and re-suspended in PBS/2% FCS for analysis on a five-laser flow cytometer (LSR FortessaTM; BD Bioscience, San Jose, CA, USA). The data were analysed with FlowJo software (TreeStar ...
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bioRxiv - Immunology 2020Quote: Cells were stimulated for 12h with SARS-CoV-2 peptide pools (0.25 μg/ml) in the presence of αCD28/αCD49d co-stimulatory antibodies (BD FastImmune™, BD Biosciences, San Diego, CA, USA), 5 μg/ml brefeldin A (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... BD 560046. After washing twice, cells were fixed in PFA 2% for 20 min, washed, and acquired on a Fortessa BD LSRII Cell Analyzer (Becton Dickinson).
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bioRxiv - Molecular Biology 2023Quote: ... PMVECs were purified after two rounds of immune selection using magnetic beads coated with anti-CD31 and anti-ICAM-2 antibodies (BD Bioscience, Franklin Lakes, NJ, USA). mPMVECs were cultured and maintained in a medium containing 5 % FBS and EC growth supplement (ECGS ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were then washed 3x in flow cytometry stain buffer and fixed with 2% PFA for 20 minutes prior to acquisition on a LSRII Fortessa (BD Biosciences, San Jose, CA, USA). Samples were analyzed with FlowJo V10 software (Figure S4).
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bioRxiv - Immunology 2023Quote: ... 5.0 × 104 CD4+ T cells were serially diluted onto either α-IL-2 or α-IFNɣ sterile coated PVDF 96 well plates (BD Biosciences, NJ, USA). The CD4+ T cells were then activated for 24 or 72 h in complete DMEM ...
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bioRxiv - Cancer Biology 2023Quote: ... or E0771 hPD-L1 (2 × 105 cells in 25 μL of medium mixed with 25 μL of Matrigel Basement Membrane Matrix [BD Biosciences, San Jose, CA, USA]) were injected into the mammary fat pad or flank ...
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bioRxiv - Genetics 2022Quote: ... The proportion of HbF-and HbS-positive RBCs were measured with an antibody recognizing HbF (Thermo Fisher for HUDEP-2 cells or BD Biosciences for primary cells) or HbS (BioMedomics) ...