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Citations for Becton, Dickinson and Company :
1351 - 1400 of 7852 citations for 6 Chloro 2 methyl 1 2 4 triazolo 4 3 b pyridazin 3 2H one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... 1×105 Tconv cells were co-cultured with Treg cells at different ratios in the presence of 2×105 irradiated APCs and 1 µg/ml soluble anti-CD3 antibody (554829, BD Biosciences). The cells were cultured in T-cell complete media which consists of RPMI 1640 medium supplemented with 50 U/ml penicillin ...
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bioRxiv - Cancer Biology 2019Quote: ... MDA-MB-231 parental or HSF1 KO with either scrambled control or DYRK2 knock-down shRNA (1 or 2) were counted and suspended in sterile phosphate buffer saline containing 50% Matrigel (BD Bioscience). 300,000 cells (100 μl ...
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bioRxiv - Immunology 2019Quote: ... We then double sorted lineage depleted cells for an HSC-enriched population (Lin- Sca-1+c-Kit+CD34+-CD135-) into 2% FBS in HBSS using fluorescent activated cell sorting (FACS) (BD Aria). Recipient mice ...
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bioRxiv - Immunology 2020Quote: ... the PBMCs were stimulated with pooled or individual peptides at a final concentration of 10 μg/mL per individual peptide for 1 h in the presence of 2 μg/mL monoclonal antibodies against human CD28 (BD Pharmingen) and CD49d (BD Pharmingen ...
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bioRxiv - Cancer Biology 2020Quote: ... For both ACHN and CAKI-1 cell lines 2×106 cells were diluted in 50 µl of PBS and 50 µl of Matrigel (BD Biosciences) and were injected subcutaneously into the right and left flank of each mouse ...
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bioRxiv - Immunology 2022Quote: ... into FACS buffer (1X PBS with 2% FBS and 1 mM EDTA) and analyzed with a FACS Aria flow cytometer (BD Biosciences).
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bioRxiv - Neuroscience 2021Quote: ... P0-P1 mouse pups were anesthetized in ice and then eyes were pierced at the edge of corneal by 30G × 1/2 needle (BD, 305106) under stereomicroscope ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Tissues were incubated at 4°C overnight with the following primary antibodies: monoclonal mouse anti-carboxyl-terminal binding protein 2 (CtBP2) IgG1 at 1:200 (612044; BD Biosciences) counterstained with goat anti-Mouse IgG1 conjugated with Alexa Fluor 568 (#A-21124) ...
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bioRxiv - Immunology 2020Quote: ... The cells were washed once with FACS buffer and fixed with 2% paraformaldehyde for 1 h prior to analyzing on an LSRII flow cytometer (BD Biosciences). In some experiments ...
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bioRxiv - Cancer Biology 2021Quote: ... samples were processed as previously described47 and 1-2 million cells were injected into cleared mammary fat pads in 10-20 microliters of commercial Matrigel (BD Biosciences). For ER+ tumors ...
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bioRxiv - Physiology 2020Quote: ... 1 ng/mL FGF2 and 10 mg/mL gentamycin) and plated on dishes previously coated with 2% Matrigel™ (BD biosciences).
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bioRxiv - Cancer Biology 2020Quote: Cells either from culture or from transwell assay were washed in PBS and incubated in 2% FBS in PBS with anti-CCR7-FITC (1:25, #561271, BD Biosciences) and anti-ROR1-APC (1:25 ...
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bioRxiv - Physiology 2022Quote: ... Novus) and F(ab’)2-donkey anti-Rabbit IgG (H+L) secondary antibody PE (1:100, 12-4739-81, BD bioscience) at 4℃ for 1h ...
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bioRxiv - Immunology 2022Quote: ... Then live Tetr-gag+Pent-gag+CD3+CD8+ cells were sorted by flow cytometry into PBS buffer 1% BSA 2 mM EDTA using a FACSAria III (BD Biosciences) equipped with 488 ...
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bioRxiv - Immunology 2023Quote: ... Cells were sorted by their distinct proliferation peaks (peak 0, peak 1 and peak 2) on FACS-Aria and Influx Cell Sorters (BD Biosciences). Cells were kept on ice and after washing with cold PBS were immediately resuspended in Trizol for RNA extraction ...
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bioRxiv - Biophysics 2022Quote: ... Saccharomyces cerevisiae strain W303-GAL4 was grown overnight in YPDA medium (yeast extract 1 %, adenine sulfate 540 μM, peptone 2 % (BD Biosciences), glucose 2 % ...
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bioRxiv - Cancer Biology 2023Quote: Single cell suspensions were pre-incubated for 15 minutes in a staining solution (PBS+/+, 2% FBS) containing 1 μg/mL Fc block (BD Biosciences), followed by an incubation with the antibodies for 45 minutes (Table S1) ...
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bioRxiv - Immunology 2022Quote: ... cells were fixed with 2% paraformaldehyde for 20 min and resuspended in 350 ul 1% BSA/PBS for phenotyping by FACSDivacytometer (BD Biosciences). To set up the compensation control ...
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bioRxiv - Biophysics 2024Quote: ... transferred in a V-bottom 96-well plates and co-cultured with K562 cells (ATCC) at 1:1 effector-to-target ratio in the presence of mouse anti-human CD107a Alexa Fluor 647 (BD Bioscience, clone H4A3, 2 uL/well) for 6 hours at 37°C ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were rinsed with PBS and incubated for 20 hours at 4°C in primary antibody solution 1:3000 mouse anti-Ki67 (BD Biosciences, San Jose, CA, USA), 2% normal horse serum ...
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bioRxiv - Microbiology 2019Quote: ... one 60-mL syringe (BD), one 1-mL syringe (Norm-Ject) ...
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bioRxiv - Microbiology 2020Quote: ... PMHs were cultured on Collagen 1 Biocoat 6-well plates (BD Biosciences) at a seeding density of 1×105cells/cm2 in Hepatocyte Growth Medium with 2% DMSO ...
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bioRxiv - Cancer Biology 2019Quote: Cells (1 × 105/well) were seeded into 6-well plates (BD Falcon). Cells were cultured for 24 h ...
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bioRxiv - Neuroscience 2023Quote: ... PE mouse anti-human Pax-6 (1:20, #561552, BD Pharmingen, USA), Alexa Fluor 647 mouse anti-Nestin (1:20 ...
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bioRxiv - Immunology 2023Quote: ... were coated with capturing anti-IL-6 (1:250, BD Biosciences, #554400) or anti-TNF (1:200 ...
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bioRxiv - Immunology 2023Quote: ... were coated with capturing anti-IL-6 (1:250, BD Biosciences, #554400) or anti-TNF (1:200 ...
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bioRxiv - Immunology 2021Quote: ... It was cultivated from Dinger’s disk in 3 mL of Elinghausen-McCullough-Johnson-Harris (EMJH) medium (BD, Difco) supplemented with 10% Leptospira enrichment EMJH medium (BD ...
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bioRxiv - Biochemistry 2019Quote: ... The plates were washed 3 times using PBST and then developed using an ELISA developing kit (BD Biosciences). The optical densities at 450 nm were read using an ELISA plate reader (Molecular Devices).
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bioRxiv - Immunology 2021Quote: ... 3 weeks) and single clones were sorted into 96-well plates using an IMI5L cell sorter (BD Biosciences). Clones were grown under selection for 3–4 wk and tested for KO by DNA sequencing and immunostaining and flow cytometry ...
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bioRxiv - Plant Biology 2020Quote: ... Laminar flow of buffer was buffer was verified with 0.1 % SPADNS and the chamber was treated with 0.05 % HPMC before IEF-FFE amphylites (pH 3-9; BD) were applied to inlets 2-6 of the chamber at a flow rate of 60 ml per hour ...
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bioRxiv - Microbiology 2019Quote: ... BMECs were seeded on the apical side of fibronectin-coated inserts (BD Falcon, 24-well, 3 µm pores). Concurrently ...
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bioRxiv - Microbiology 2020Quote: ... Proteins bound to the column were eluted with 3 ml Elution buffer (BD buffer with 100 mM imidazole) and aliquoted in 300 μl fractions ...
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bioRxiv - Microbiology 2022Quote: ... Proteins bound to the column were eluted with 3 ml elution buffer (BD buffer with 100 mM imidazole) and aliquoted in 300 μl fractions ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were then transferred onto a nitrocellulose membrane which was then blocked in 3% skimmed milk (BD Difco). Membranes were then subjected to immunoblotting ...
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bioRxiv - Cancer Biology 2021Quote: ... GFP-expressing PC-3 cells were sorted by FACS Aria III (BD Life Sciences, Franklin Lakes, NJ, USA) and maintained with complete medium ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... 36 cm above the arena an infrared-sensitive Chameleon 3 camera (CM3-U3-13S2C-CS-BD, FLIR, USA) was used to record fly movements ...
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bioRxiv - Immunology 2021Quote: We have used 3 BD FACSAria™ Fusion cell sorters (Fusion) and a FACSSymphony A5 (Symphony) from BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... Wells were extensively washed again and developed with 3-amino-9-ethyl-carbazole (AEC) substrate set (BD Biosciences). After drying ...
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bioRxiv - Immunology 2021Quote: bEnd.3 cells were cultured to 80% confluence then seeded onto transwells (8 µm pore size; BD Biosciences) and grown for 5 d until they reached polarisation ...
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bioRxiv - Cancer Biology 2021Quote: PCa cells (3 × 105) in serum-free medium were seeded into FluoroBlock TM Cell Culture insert (BD Falcon). The lower chamber contained conditioned media (CM ...
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bioRxiv - Cell Biology 2022Quote: Cells were harvested by centrifugation at 1600 rpm for 3 minutes and fixed in Cytofix Fixation Buffer (BD) for 10 minutes at 37 °C ...
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bioRxiv - Immunology 2022Quote: ... for 3 hours at 37°C in the presence of GolgiStop (monensin, according to manufacturer’s recommendation, BD Biosciences). Cells were washed and labelled with fixable viability dye (LIVE/DEAD eFlour780 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells were subsequently stained with the rabbit anti-active caspase-3 antibody (clone C92-605) (BD Biosciences, USA) in the dark ...
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bioRxiv - Immunology 2023Quote: ... Left lung lobes were homogenized in 3 mL MilliQ water containing PANTA™ Antibiotic Mixture (BD, cat.no. #245114) using GentleMACS M-tubes (Miltenyi Biotec) ...
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bioRxiv - Molecular Biology 2023Quote: ... All samples (24 samples; 8 samples per macrophage polarization state) were cleaned (Supplementary Figure 3; FlowJo, version 10.7.1, BD), and live single cells were imported into OMIQ (Omiq.ai ...
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bioRxiv - Immunology 2023Quote: ... antibody mix 3 (Supplementary Table S4) with Sample Tag oligonucleotide-conjugated antibodies (Single-Cell Multiplexing Kit, BD Biosciences) was added to the cell suspension and left on ice for 15 minutes for staining ...
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bioRxiv - Cancer Biology 2023Quote: ... This was followed by overnight incubation with the primary antibody (Purified Rabbit Anti-active caspase 3 (BD 559565) 1:200 in Block solution ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were then sorted for mCherry-positive cells (cutoff set at 99.9th percentile of the untransduced cell population’s mCherry signal) using a FACSAria 3 cell sorter (BD). We observed 4% of cells with a mCherry positive signal ...
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bioRxiv - Developmental Biology 2024Quote: ... Red blood cells were lysed twice on ice for 3 min with BD Pharm Lyse Buffer (BD Pharmingen). Identification of leukocyte subsets was performed by staining with antibodies ...
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bioRxiv - Immunology 2023Quote: ... Cells were stimulated with 0.5ug/ml purified NA/LE hamster anti-mouse-CD40 (clone HM40-3 BD Pharmingen), 10ug/ml affinity pure F(ab)2 fragment goat anti-mouse-IgM ...