3 Hydrazino 5 methyl 4H 1 2 4 triazol 4 ylamine hydrochloride citations for Becton, Dickinson and Company

Quote: ... 1x105 tumor cells and 1x105 γδ T cells were co-cultured for 3 h at 37°C in the presence of 1 µl α-CD107a-PE antibody (BD Biosciences). Medium or stimulation with α-CD3 and α-CD28 (both 1 µg/ml ...

A novel Mannan-specific chimeric antigen receptor M-CAR redirects T cells to interact with Candida spp. hyphae and Rhizopus oryzae spores

Júlia Garcia Guimarães, et al., bioRxiv - Bioengineering 2024

Quote: The expression of the exhaustion markers PD-1 and TIM-3 was evaluated using anti-PD1 (cat. 560795, BD Biosciences, BD PharMingen) and anti-TIM3 (cat ...

Lotus japonicus karrikin receptors display divergent ligand-binding specificities and organ-dependent redundancy

Samy Carbonnel, et al., bioRxiv - Plant Biology 2020

Quote: ... thaliana seedlings were grown for 5 days on half-strength Murashige and Skoog (MS) medium containing 1% agar (BD). L ...

A suitable murine model for studying respiratory coronavirus infection and therapeutic countermeasures in BSL-2 laboratories

Ana Cláudia dos Santos Pereira Andrade, et al., bioRxiv - Microbiology 2021

Quote: ... 1×106 cells were blocked with mouse BD FC block™ (5 μg/mL, BD Pharmingen, Cat No. 553141) and then stained using fluorescent-labeled monoclonal antibodies as follows ...

C/EBPB-dependent Adaptation to Palmitic Acid Promotes Tumor Formation in Hormone Receptor Negative Breast Cancer

Xiao-Zheng Liu, et al., bioRxiv - Cancer Biology 2021

Quote: ... cells were washed once with 5 mL of PBS/1% BSA and analyzed on flow cytometry (BD LSR Fortessa). To gate the CD44high/CD133+ cell population ...

Interleukin 7 receptor drives Early T lineage Progenitor expansion

Rafael A. Paiva, Vera C. Martins, bioRxiv - Immunology 2021

Quote: ... for 1 hour at −20 ºC and finally stained for phosphorylated Stat-5 (BD biosciences, clone 47/Stat5; a647) in PBS/10% FBS for 30 min at room temperature ...

Sensitivity of dairy calf Salmonella enterica serotype Cerro isolates to infection-relevant stressors

Sarah M. Raabis, et al., bioRxiv - Microbiology 2024

Quote: ... Approximately 1 g of feces or 5 g of each tissue sample were added to tetrathionate broth (BD DifcoTM) and were incubated overnight at 37°C ...

Crosstalk of platelets with macrophages and fibroblasts aggravates inflammation, aortic wall stiffening and osteopontin release in abdominal aortic aneurysm

MU Wagenhäuser, et al., bioRxiv - Molecular Biology 2023

Quote: ... GPIIIa, Luc.H11-FITC, #M031-1 or Integrin ⍺ 5/CD49e, Tap.A12-FITC and anti-human CD61-PE, #555754, BD Biosciences) and incubated for 15 min at RT in the dark ...

An inducible genetic tool for tracking and manipulating specific microglial states in development and disease

Kia M. Barclay, et al., bioRxiv - Neuroscience 2023

Quote: ... cells were resuspended in FACS buffer and incubated in FC Block for 5 minutes (1:60, BD Pharmingen, 553142) and primary antibodies for 10 minutes ...

The PIP4K2 inhibitor THZ-P1-2 exhibits antileukemia activity by disruption of mitochondrial homeostasis and autophagy

Keli Lima, et al., bioRxiv - Pharmacology and Toxicology 2022

Quote: ... Cells were then washed with PBS and resuspended in buffer assay red containing 5 μg/mL JC-1 (BD) and 10 000 events were acquired by flow cytometry (FACSCalibur ...

Type 1 lymphocytes and interferon-γ accumulate in the thalamus and restrict seizure susceptibility after traumatic brain injury

Nicholas M. Mroz, et al., bioRxiv - Immunology 2024

Quote: ... heat-inactivated FBS and 0.05% NaN3) containing 5% (v/v) normal rat serum and Fc Block (2.4G2, 1:250, BD Biosciences) for 20 min at 4°C ...

Inhibition of glutaminase elicits senolysis in therapy-induced senescent melanoma cells

Justin Kim, et al., bioRxiv - Cancer Biology 2024

Quote: ... 100 μl of the suspension (1×105 cells) was incubated with 5 μl of APC Annexin V (BD Biosciences) and incubated for 15 minutes at room temperature ...

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