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Citations for Becton, Dickinson and Company :
1301 - 1350 of 1567 citations for Recombinant Human MAP1LC3A Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: Specificity of neoantigen-reactive CD8+ T-cells and TCR-transduced T-cells was assessed by human IFNγ (BD Biosciences Cat. #555142), IL-2 (BD Biosciences Cat ...
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bioRxiv - Neuroscience 2023Quote: ... and tumor necrosis factor (TNF) in cell supernatants were measured using the Human Inflammatory Cytokine Cytometric Bead Array Kit (BD Biosciences). Concentrations of chemokines in cell supernatants were measured using the LEGENDplexTM Human Proinflammatory Chemokine Panel 1 (Biolegend) ...
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bioRxiv - Genomics 2024Quote: ... day 7 cells were transferred to fresh culture medium with IL-2 and stimulated again with peptides and 1ug/mL of anti-human CD28 antibody (BD Biosciences) for another 7 days ...
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bioRxiv - Cell Biology 2024Quote: ... IL-6 and IL-1β cytokines were measured in the cell culture supernatant after 2 h of co-exposure using CBA human Inflammatory cytokine kit (BD Biosciences), while chemokines CXCL8/IL-8 ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were then spun down and resuspended in 100LμL of buffer containing fluorescently conjugated antibodies for one hour (5LμL of each antibody per sample): APC-Cy7 Mouse Anti- Human CD4 (1:20 dilution; BD Biosciences, #557871), APC-Cy7 Mouse IgG1 ...
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bioRxiv - Microbiology 2023Quote: ... for 16 hours followed by stimulation with a peptide pool of a broad range of HLA-subtypes and infectious agents (CEFX Ultra SuperStim Pool; JPT) (1 µg/ml) and 1 μg/ml mouse-anti-human CD28 antibody (#556620; BD Biosciences), or IL-2 (20 IU/ml ...
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bioRxiv - Biophysics 2024Quote: ... cells were washed twice in PBS + 2% FBS and stained with mouse anti-human CD45 Pe-Cy5 (BD Bioscience, clone HI30) and mouse anti-human CD107a Alexa Fluor 647 for 30 minutes at 4°C in the dark ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then washed 2x with 1% FBS in PBS and incubated in 1% FBS in PBS with APC-conjugated mouse anti-human CD68 (BD Biosciences) for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... bone marrow aspirates were performed every 6 weeks post-transplant and human chimerism was tracked by flow cytometry analysis (using a BD LSR Fortessa analyzer or BD FACSymphony) and analyzed with the following ...
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bioRxiv - Cancer Biology 2021Quote: ... and resuspended in 50 µL of FACS buffer (PBS plus 1% BSA) and blocked with µL 1 human Fc block (BD Biosciences, 564219) for 20 minutes at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Flow cytometry was conducted and the supernatant was collected from each well to assess IFNγ and CCL5 levels using a Human IFNγ ELISA Set (BD OptEIA, 555142) and ELISA MAX™ Deluxe Set Human CCL5 (Biolegend ...
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bioRxiv - Cancer Biology 2020Quote: ... Then cells were blocked by PBS plus 5% BSA and later incubated with FITC Anti-Human CD24 (BD Biosciences, San Jose, CA), and PE Anti-Human CD44 (BD Biosciences) ...
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bioRxiv - Immunology 2021Quote: ... 1 mM ethylenediaminetetraacetic acid (EDTA)) with the following anti-human antibodies (all at 1:200 dilution): anti-CD20-PECy7 (BD Biosciences, 335793), anti-CD3-APC-eFluro 780 (Invitrogen ...
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bioRxiv - Bioengineering 2021Quote: ... To assess activation marker status T cells were stimulated with 0.1 μg/mL of D117C or PIGF on collagen coated plates for 16-20 hr and surface-stained with anti-human CD3 BUV395 (BD clone SK7), CD4 BV785 (Biolegend clone RPA-T4) ...
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bioRxiv - Microbiology 2020Quote: ... the cells were washed twice and incubated with an R-phycoerythrin (PE)- conjugated mouse anti-human IgG1 secondary antibody (BD Biosciences, 555787) on ice for 30 minutes ...
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bioRxiv - Immunology 2022Quote: ... 1 mM ethylenediaminetetraacetic acid (EDTA)) with the following anti-human antibodies (all at 1:200 dilution): anti-CD20-PECy7 (BD Biosciences, 335793), anti-CD3-APC-eFluro 780 (Invitrogen ...
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bioRxiv - Bioengineering 2020Quote: Seven days after electroporation a total of 5 × 106 T cells were collected and stained in 100 uL with fluorophore-conjugated anti-human antibodies for CD3 (BD Biosciences #564001), beta-2-microglobulin (BioLegend #316306 ...
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bioRxiv - Physiology 2022Quote: Genome-edited hiPSCs were evaluated for expression of pluripotency markers using the BD Human Pluripotent Stem Cell Transcription Factor Analysis Kit (BD Biosciences, UK). In vitro differentiation efficiency was assessed by expression of stage-specific markers of definitive endoderm (CXCR4 ...
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Mutation in the matricellular gene fibulin-4 leads to endothelial dysfunction in resistance arteriesbioRxiv - Physiology 2022Quote: ... Membranes were blocked in 5% BSA/0.1% Tween20 in 1x TBS or 5% milk/0.1% Tween20 in 1x PBS for primary antibody mouse anti-human phospho-eNOS (1:1,000, pS1177, #612392, BD Biosciences, Franklin Lakes, NJ) or mouse anti-human-eNOS (1:1,000 ...
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bioRxiv - Immunology 2022Quote: ... 1 mM ethylenediaminetetraacetic acid (EDTA)) with the following anti-human antibodies (all at 1:200 dilution): anti-CD20-PECy7 (BD Biosciences, 335793), anti-CD3-APC-eFluro780 (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... washed with PBS and incubated in DMEM media containing 1% FBS and 1% BSA with Alexa Fluor® 647 Mouse Anti-Human LAMP1 Clone H4A3 (BD Biosciences) for 1h at 4°C with intermittent mixing ...
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bioRxiv - Cancer Biology 2022Quote: ... Apoptotic cells in leukemic spleens or human/mouse cell lines after different treatments were quantified with PE-AnnexinV Apoptosis Detection Kit I (BD Pharmingen, 559763). All flow cytometry data was collected on an Attune NxT Flow Cytometer (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... HAECs were allowed to recover from passaging for 24 h after plating and were subsequently incubated with AlexaFluor-647 anti-human VE-Cadherin antibody (1:100, BD Biosciences, #561567). After 15 minutes incubation ...
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bioRxiv - Cancer Biology 2022Quote: ... Human whole blood was collected from adult healthy volunteers with prior consent in heparinized vacutainer blood collection tubes (BD, Franklin Lakes, NJ) in accordance with Ahir et al. ...
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bioRxiv - Cancer Biology 2019Quote: 5×105 human CD14+ cells were isolated from peripheral blood mononuclear cells (PBMC) using anti-CD14 beads (BD Biosciences, San Jose, CA) with a typical yield of ≥ 70% recovery and ≥ 90% purity ...
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bioRxiv - Immunology 2019Quote: ... Immune cells were purified by FACS isolation of CD45 positive cells using APC Mouse Anti-Human CD45 (BD Pharmingen, clone: HI30 (RUO), American ...
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bioRxiv - Biochemistry 2020Quote: ... and CD81 antibodies (with no beads) were added to the solution containing the EVs (PE Mouse Anti-Human CD81 Clone JS-81, BD Pharmingen™). After 1 h of incubation ...
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bioRxiv - Immunology 2021Quote: ... Macaque cells were incubated with 100 μl of FACS buffer (PBS 1x with 2% fetal bovine serum and 1mM EDTA) with human Fc Block (BD Biosciences #564219) at a 1:500 dilution for 30 min on ice.
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bioRxiv - Immunology 2021Quote: ... and viability dye (eBioscience™ Fixable Viability Dye eFluor™ 780) followed by Foxp3 staining with anti-FoxP3 (259D/C7) using Human FoxP3 buffer set (BD biosciences) according to the Manufacturer’s recommendations.
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bioRxiv - Immunology 2021Quote: ... spleen and bone marrow were isolated and evaluated for the percentage of human CD45+ cells by flow cytometry (CD45-PerCP-Cy5.5, Biolegend Cat: 304028; BD FACS Canto II). Populations were defined and analyzed using FlowJo software (Flowjo v10.7.1 ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was collected after 6 days of co-culture to assess IFNγ and CCL5 levels using a Human IFNγ ELISA Set (BD OptEIA, 555142) and ELISA MAX™ Deluxe Set Human CCL5 (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... a panel of four fluorescent markers was employed including fluorescein isothiocyanate (FITC) mouse anti-human CD40 (BD Biosciences; San Jose, CA, USA), phycoerythrin (PE ...
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bioRxiv - Immunology 2022Quote: Secretion of CXCL10 (IP-10) into the medium was quantified 24 h after stimulation by ELISA (Human IP-10; BD OptEIA, 550926). Briefly ...
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bioRxiv - Immunology 2022Quote: ... 1 mM ethylenediaminetetraacetic acid (EDTA)) with the following anti-human antibodies (all at 1:200 dilution): anti-CD20-PECy7 (BD Biosciences, 335793), anti-CD3-APC-eFluro 780 (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were incubated in media containing 10 µg/mL blocking antibody (Purified Rat Anti-Human CD29 Clone Mab 13, BD Biosciences, #552828) or equivalent concentration non-targeting isotype control (Purified Rat IgG2a κ Isotype Control Clone R35-95 ...
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bioRxiv - Bioengineering 2023Quote: ... at 10 × 106 cells/mL followed by incubation with antibodies against human CD44 (APC-conjugated, Clone G44-26, 1:5, BD Biosciences, US) and CD24 (PE-Cy7-conjugated ...
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bioRxiv - Cell Biology 2023Quote: ... VE-cadherin was stained 1:200 with the live labeling antibody Alexa Fluor® 647 Mouse Anti-Human CD144 (BD Pharmingen™), 2 min prior to imaging ...
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bioRxiv - Immunology 2023Quote: ... 96-well plates were pre-coated with 10 ng/mL anti-CD3 mAb at 4°C overnight (Purified NA/LE mouse anti-human CD3, BD Cat# 557052). Three-fold serial dilutions of either IL-7 or a test compound were added to the cells and incubated for 4 days at 37°C ...
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bioRxiv - Immunology 2023Quote: ... A target primer panel was constructed by combining a commercial predesigned Human T Cell Expression Primer Panel (BD Biosciences, 259 target primers) with our custom-designed primer panel (BD Biosciences) ...
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bioRxiv - Immunology 2023Quote: The purity of each NK population was checked before and after cell sorting and routinely by flow cytometry with anti-human CD16-BV421 (clone 3G8, BD Pharmagen, France), anti-human CD56-PeCy7 (clone B159 ...
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Tracer-based metabolomics for profiling nitric oxide metabolites in a 3D microvessel-on-a-chip modelbioRxiv - Cell Biology 2023Quote: ... Permeabilization was followed by blocking cells using 5% BSA in HBSS+ for 30 min and incubated with the primary antibody solution - Mouse anti-human CD144 (1:150; 555661, BD Biosciences, USA) for overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... and co-cultured with human CD8+ T cells at 1:1 E:T ratio in the presence of anti-CD107a-APC mAb (BD Biosciences, CA, USA). After 1 hour ...
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bioRxiv - Immunology 2023Quote: ... Cytometric Bead Array using enhanced sensitivity flex sets of human IL-2/IFN- γ/TNF- α was done following manufacturer’s instruction (BD Biosciences). Alternatively ...
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bioRxiv - Immunology 2023Quote: ... were diluted 1:50 in PBS-10% FBS and IL-2 concentration was determined by ELISA assays using the OptEIA Human IL-2 ELISA commercial kit (BD Biosciences, USA) and revealed with TMB substrate (Thermofisher ...
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bioRxiv - Immunology 2020Quote: ... and proteins surface expression assessed by Accuri C6 Plus flow cytometry (BD Bioscience, Oxford, UK). Cytokines and chemokines were measured in the cell media supernatant by ELISA (Peprotech) ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells expressing fluorescence proteins were collected uisng a FACS Aria III flow cytometer (BD Biosciences), and used for downstream experiments.
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bioRxiv - Neuroscience 2021Quote: ... C-terminal-binding protein 2 / RIBEYE ribbon synapse maker (Schmitz et al., 2000) (CtBP2, BD Bioscience ...
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bioRxiv - Immunology 2020Quote: Surface marker proteins and intracellular cytokine responses were quantified via flow cytometry (LSRII, BD Biosciences) and analyzed using FlowJo software version 10.5.3 (TreeStar Inc. ...
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bioRxiv - Immunology 2021Quote: ... Cell surface expression of adhesion molecule protein was measured by multiparameter flow cytometry (BD Fortessa). Supernatants were retained and secreted chemokines were measured by ELISA (IP-10 ...
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bioRxiv - Immunology 2020Quote: ... Expression of proteins was measured using FACS Canto II HTS (BD Biosciences, Bedford, MA, USA) and analyzed using FlowJo software (FlowJo LLC ...