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Citations for Becton, Dickinson and Company :
1301 - 1350 of 1783 citations for Recombinant Human Complement Factor H His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... The following day thawed human B cells were stained with CD19-BV510 (clone 5J25C1, BD Biosciences), CD27-PE-Cy7 (clone 0323 ...
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bioRxiv - Immunology 2021Quote: ... and negative for CD11c (APC mouse anti-human CD11c, clone B-ly6, BD Pharmingen Cat#559877), and subsequently analyzed for the expression of pDC markers CD123 (PE mouse anti-human CD123 ...
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bioRxiv - Immunology 2021Quote: ... PBMCs and MNC suspensions were blocked with 6.25 μg/ml anti-human CD4 antibody (BD Biosciences) at 4°C for 15 min followed by staining with a panel of fluorochrome-conjugated antibodies to identify antigen-specific memory B cells as described by gating strategy (Supplemental figure 2B) ...
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bioRxiv - Genomics 2019Quote: ... The antibodies used were: phycoerythrin (PE)- mouse anti-human anti–CD41a antibody (BD Pharmingen; Cat# 555467); fluorescein isothiocyanate (FITC)- mouse anti-human Annexin-V (biolegend ...
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bioRxiv - Immunology 2022Quote: Enzyme-linked immunospot (ELISpot) assays were conducted using the Human IFN-γ ELISpot Set (BD Biosciences) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... cells were stained with TLR1 (BV421 conjugated Mouse Anti-Human CD281, clone: GD2.F4, BD Biosciences), TLR2 (FITC conjugated anti-human CD282 Antibody ...
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bioRxiv - Immunology 2023Quote: ... cells were spun down and resuspended in 50μL of human Fc block (BD Biosciences Cat. 564220) in DPBS and incubated for 10 minutes at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... samples were blocked with Human BD Fc Block™ solution (1 µL/200,000 cells) (BD Biosciences) for 10 minutes at room temperature to reduce any non-specific antibody staining by Fc Receptors expressed on macrophages ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells were incubated with purified mouse anti-human Galectin-3 (1:100 dilution, #556904, BD Biosciences) diluted in blocking buffer for 1hr at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were then incubated in a blocking solution containing Human BD Fc Block (564220; BD Biosciences) diluted 1:25 (v/v ...
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bioRxiv - Neuroscience 2023Quote: ... Expression of MSCs markers was confirmed using the FACS Human MSC Analysis Kit (AB_2869404, BD Bioscience) according to the manufacturer instruction ...
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bioRxiv - Cancer Biology 2023Quote: ... BV450 anti-human CD31 (Clone WM59, dilution 1/20, BD Biosciences, Le Pont de Claix, France), PE anti-human MSCA1 (Clone W8B2 ...
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bioRxiv - Cancer Biology 2023Quote: ... APC anti-human CD206 (clone 19.2, dilution 1/10, BD Biosciences, Le Pont de Claix, France) and PE-Vio® 770 anti-human CD14 (clone TÜK4 ...
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bioRxiv - Cancer Biology 2023Quote: ... PerCP anti-human CD34 (Clone 8G12, dilution 1/20, BD Biosciences, Le Pont de Claix, France), BV450 anti-human CD31 (Clone WM59 ...
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bioRxiv - Cancer Biology 2023Quote: ... PerCP anti-human CD8 (Clone SK1, dilution 1/10, BD Biosciences, Le Pont de Claix, France) and APC anti-human CD25 (Clone 2A3 ...
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bioRxiv - Developmental Biology 2023Quote: ... hRGCs Brn3b-tdT were treated with human IgG or anti-DCC antibody (10μg/ml; BD Biosciences) or anti-Robo2 antibody (20μg/ml ...
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bioRxiv - Immunology 2023Quote: ... and the following conjugated anti-human monoclonal antibodies (mAbs): anti-CD4-FITC (M-T477, BD Pharmingen), anti-CD8-PerCP (SK1 ...
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bioRxiv - Molecular Biology 2023Quote: ... using the BD IMag Anti-Human CD14 Magnetic Particles – DM (BD Biosciences, San Jose, CA, USA), according to the instructions given by the supplier ...
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bioRxiv - Immunology 2023Quote: ... IP-10 in tissue culture supernatants was quantified with a Human IP-10 ELISA Set (BD).
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bioRxiv - Cancer Biology 2023Quote: ... Engraftment was measured by flow cytometry for human CD45+ cells (BD no. 571875, dilution 1:100). All animal studies were done at the University of Colorado under Institutional Animal Care and Use Committee– approved protocol no ...
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bioRxiv - Cancer Biology 2023Quote: ... Engraftment was measured by flow cytometry for human CD45+ cells (BD no. 571875, dilution 1:100). All animal studies were done at the University of Colorado under Institutional Animal Care and Use Committee– approved protocol no ...
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bioRxiv - Cancer Biology 2023Quote: ... and mouse anti-human αvβ3 (CD51/CD61)-FITC (Clone 23C6; BD Pharmingen, San Jose, CA, USA). Isotype controls included rat IgG2b-APC (Clone eB149/10H5 ...
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bioRxiv - Neuroscience 2023Quote: ... and blocked for 15 minutes with 1:20 human FC block (BD Biosciences; Cat. No. 564220) and then stained with 1:66 PE-Cy7 anti-human CD71 (TFRC ...
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bioRxiv - Cell Biology 2024Quote: ... CCL2/MCP-1 and CXCL10/IP-10 were quantified using CBA human chemokine kit (BD Biosciences), according to the instructions from the manufacturer ...
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bioRxiv - Cell Biology 2024Quote: Human platelets were isolated from ACD-A anticoagulated whole blood (collected in BD Vacutainer ACD-A) as previously described.26 The platelet pellet was resuspended in HEPES-buffered Tyrodes buffer with BSA 0.35% (w/v ...
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bioRxiv - Molecular Biology 2024Quote: ... H1 human ESC lines (WA01, WiCell) were maintained in mTeSR plus (STEMCELL) on Matrigel (BD Biosciences) and passaged using TryplE in single cells ...
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Resolution of SARS-CoV-2 infection in human lung tissues is driven by extravascular CD163+ monocytesbioRxiv - Immunology 2024Quote: ... The following anti-human antibodies were used for flow cytometry and were all acquired from BD Biosciences (San Jose ...
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bioRxiv - Cancer Biology 2024Quote: ... as well as pe-cy-conjugated mouse anti-human CD86 (5 μL/106 cells; BD Biosciences) were incubated on ice for 30 minutes ...
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bioRxiv - Immunology 2021Quote: ... Cells were then incubated with pS6 or HK2 antibody for 1 h and O-GlcNAc antibody for 20 min at RT in the dark in cytoplasmic permeabilization buffer (BD Biosciences). Nuclear permeabilization buffer was used for c-Myc staining ...
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bioRxiv - Genetics 2022Quote: ... Cells were incubated at 37°C with 5% CO2 for 24 h and then sorted for GFP+ expression using fluorescent activated cell sorting (FACS) on a FACSAria II (BD Bioscience). The GFP+ pool (bulk ...
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bioRxiv - Immunology 2022Quote: ... cells were stimulated for 4 h with eBioscience™ Cell Stimulation Cocktail and a protein transport inhibitor-containing Brefeldin (1.5uL/mL StopGolgi; BD Biosciences). Cells were then washed in FACS buffer ...
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bioRxiv - Immunology 2021Quote: ... the killing capacity of NK cells was tested when they coculture with senescent / young or modulated for ST8SIA1 expression MRC5.NK cells are added to the culture for 4 h in presence of monensin and brefeldin (BD Biosciences) at the effector/target ratio of 1:1 ...
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bioRxiv - Bioengineering 2021Quote: ... H-2Kb-negative GFP-positive cells were sorted into single cells on a 96-well plate using FACSAria Fusion (BD Biosciences) and cultured for 2-3 weeks in D10 ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were collected at 72 h post FeNPs administration and detected with the Annexin V-FITC Apoptosis Detection Kit (BD, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The percentage of GFP-positive cells among the mCherry-positive cells was determined 48 h after I-SceI transfection using a LSRFortessa X-20 (BD Bioscience) using BD FACSDiva Software v8.0.1 ...
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bioRxiv - Microbiology 2020Quote: ... lymphocytes were cultured in 96-well dishes at 37°C for 5-6 h in the presence of 2 μM peptide pool and brefeldin A (BD Biosciences). Cells were then labeled for cell-surface markers ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were then incubated for 72 h at 4°C in mouse anti-PKCδ primary antibody (1:1000, BD Biosciences, 610397) in blocking solution containing 1.5% NGS ...
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bioRxiv - Cancer Biology 2019Quote: ... the cells were re-stimulated for another 6 h with the anti-CD3 antibody in the presence of both GolgiStop and GolgiPlug (BD Biosciences) and stained with antibodies against IFN-γ and TNF-α ...
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bioRxiv - Genomics 2020Quote: ... 3 times 5 min washes with TNT were followed by a 1 h incubation with biotinylated goat anti-rat (559286, BD biosciences) 1:100 in TNB ...
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bioRxiv - Bioengineering 2022Quote: ... Genes encoding the proteins were cloned into pET29b between the restriction sites NdeI and XhoI and expressed for 4 h in Terrific Broth (BD Difco) using Lemo21 (NEB ...
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bioRxiv - Microbiology 2022Quote: ... aureus was also grown in cell free supernatants (CFS) of Fusobacterium nucleatum ATCC 25586 that had been cultured anaerobically for 48 h in BBL Brucella Broth (BD 2011088) supplemented with 250 μg/mL and 50 μg/mL of hemin and vitamin K (Hardy Diagnostics Z237) ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were allowed to activate for 24 and 48 h and then stained with Annexin V FITC and 7AAD (BD Pharmingen). Cell proliferation was analyzed 96 hours post polyclonal activation in CD4+ T cells previously stained with cell trace violet dye (Thermo Fisher).
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bioRxiv - Molecular Biology 2020Quote: ... the cells were treated with 1 µg/ml doxycycline for 24 h and single GFP+ cells were sorted into 96-well plates on a BD FACSAria UV instrument (BD Biosciences) and grown until colonies formed.
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bioRxiv - Cancer Biology 2020Quote: ... cells were stimulated with 100 ng/ml phorbol 12-myristate 13-acetate (PMA) plus 1 µg/ml ionomycin for 4 h at 37°C in the presence of GolgiPlug/GolgiStop (BD Pharmigen). Cells were stained for surface molecules as described above ...
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bioRxiv - Immunology 2020Quote: ... The cells were stained with fluorochrome-conjugated antibodies or influenza HA-specific (HA518–526) H-2Kd tetramer-IYSTVASSL (MBL) reagent and analyzed using a flow cytometer (BD Biosciences). BMDCs (105 cells ...
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bioRxiv - Immunology 2020Quote: ... The cells were washed once with FACS buffer and fixed with 2% paraformaldehyde for 1 h prior to analyzing on an LSRII flow cytometer (BD Biosciences). In some experiments ...
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bioRxiv - Microbiology 2021Quote: ... lymphocytes were cultured in 96-well dishes at 37°C for 5-6 h in the presence of 2 μM peptide pool and brefeldin A (BD Biosciences). Cells were then labeled for cell-surface markers ...
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bioRxiv - Immunology 2021Quote: ... lamina propria cells were stimulated for 5 h with PMA (50 ng/ml) and ionomycin (500 ng/ml) in the presence of BD Golgi plug (BD Biosciences) followed by fixation and permeabilized using the BD Cytofix/Cytoperm kit ...
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bioRxiv - Cell Biology 2019Quote: ... GFP+ cells were sorted in 96-well plates 48 h post transfection using a FACS Aria III cell sorter (BD BioSciences). Single cells were grown until formation of viable individual clone in DMEM medium supplemented with 50% fetal bovine serum and 1% penicillin/streptomycin ...
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bioRxiv - Microbiology 2020Quote: ... Footpads were digested with 4 mg ml−1 collagenase D for 2 h at 37°C before dissociation through 70 μm cell strainers (BD Biosciences). Homogenates were resuspended in HOMEM supplemented with 20% FCS and serial dilutions (2-fold ...