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Citations for Becton, Dickinson and Company :
1301 - 1350 of 2491 citations for Phosphatase Inhibitor Cocktail 2 Tubes 100X since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Then, GolgiPlug and GolgiStop (brefeldin A and monensin, respectively) protein transport inhibitors (BD Biosciences) were added ...
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bioRxiv - Immunology 2021Quote: ... The protein transport inhibitor monensin (GolgiStop, Cat#51-2092KZ, BD Biosciences, San Jose, CA) was added to the wells to prevent cytokine secretion 60 minutes after the addition of the peptides ...
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bioRxiv - Molecular Biology 2022Quote: ... Passaging was performed using mTeSR-1 supplemented 1 nM ROCK inhibitor (BD Biosciences 562822) to prevent differentiation ...
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bioRxiv - Developmental Biology 2021Quote: ... was flushed 2-3 times with sterile PBS using a 26G x 1/2 needle and 1 ml syringe (BD). BM-free bones were cut into small pieces with sterile scissors until bones became paste (usually 3 mins for 3 weeks old and 5 mins for 6-9 weeks old) ...
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bioRxiv - Immunology 2019Quote: ... washed with PBS containing 2% FBS and 2 mM EDTA and taken for analysis on a flow cytometry machine (FORTESSA X-20, BD).
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bioRxiv - Cancer Biology 2021Quote: ... followed by incubation in blocking buffer (1% anti-mouse-CD16/CD32, 2.4 G2, 2% FBS, 5% NRS,1% triton x100 and 2% NMS in HBSS; BD Bioscience) for 1h at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... Adherent and suspension cells of each sample were pooled and after centrifugation for 5min at 800 × g cells were carefully resuspended in annexin V staining buffer (RPMI1640 without phenol red containing 1 % FBS, 2 µg/ml lysostaphin, 2 mM CaCl2, 10 µl/ml annexin V-APC [BD Biosciences] and 10 µl/ml 7AAD [BD Biosciences]) ...
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bioRxiv - Immunology 2019Quote: ... The cells were then incubated for 18 hours and IL-2 amounts in the supernatant were determined using a mouse IL-2 OptEIA ELISA kit from BD Biosciences as per manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... Samples were then washed with 2% and stained with 2% FCS in PBS and subsequently washed stained with goat anti-rabbit IgG –FITC secondary (BD) for 30 minutes at RT ...
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bioRxiv - Cell Biology 2020Quote: ... and interleukin-2 cytokine production was measured using BD optiEA Mouse IL-2 ELISA set following the manufacturer’s instructions (BD Biosciences).
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bioRxiv - Immunology 2022Quote: ... Cells were washed twice with 2% FBS in PBS and resuspended in 2% formalin before being acquired on an LSRFortessa (BD). Data was collected in the FITC and scatter channels ...
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bioRxiv - Microbiology 2022Quote: ... venezuelae ATCC 10712 was cultured in liquid complete supplement mixture (CSM) medium or on International Streptomyces Project medium 2 (ISP-2) agar (277010, BD). To make CSM medium base ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 x 2 cm of dorsal skin tissue was processed for skin MPC isolation by FACS (BD FACS AriaTM III). For defining the gates for GFP+ and Tomato+ cells ...
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bioRxiv - Systems Biology 2023Quote: ... all strains are grown in YPD (2% w/v Bacto Peptone, BD Biosciences #211677; 2% w/v D-glucose, BD Biosciences #215520 ...
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bioRxiv - Neuroscience 2023Quote: ... All dissociated cells were blocked with Fc receptors staining buffer (1% anti-mouse CD16/CD32, 2.4 G2, 2% FBS, 5% NRS, and 2% NMS in HBSS; BD Bioscience) and then stained with a standard panel of antibodies ...
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bioRxiv - Neuroscience 2023Quote: ... We performed a post-hoc exclusion of 4 of the samples based on their high heterogeneity observed by PCA plot (n = 2 CT and n = 2 BD), as compared with the 14 other samples ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were incubated with a lineage antibody cocktail (B220, CD3, CD4, CD8, Gr1, CD11b, NK1.1, Il7R, CD19) and were sorted on FACSAria II sorter (BD Biosciences) to recover GMPs ...
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bioRxiv - Immunology 2019Quote: ... were labeled with a cocktail of non-APC binding antibodies namely the murine T-cell lineage directed anti-Thy-1.2 antibody (BD Pharmingen) and the T-cell subsets recognizing CD4 antibody (eBioscience ...
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bioRxiv - Immunology 2020Quote: ... single-cell suspensions of spleen and lung tissue and blood at a concentration of 5 x 106 cells/mL were treated with RPMI plus 5 µL/mL Leukocyte Activation Cocktail with GolgiPlug (BD) or RMPI only and incubated for 4 hours at 37 °C ...
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bioRxiv - Microbiology 2022Quote: Cells were incubated in 50 μL of FACS buffer containing a cocktail of antibodies for 30 minutes on ice prior to washing and analysis on a FACS Aria (BD). Antibodies included anti-IgM FITC (G20–127 ...
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bioRxiv - Developmental Biology 2020Quote: Single-cell suspensions of mouse mammary epithelial cells were blocked in Fc block cocktail (FACS buffer, 6.25 μg/mL Mouse BD Fc block (BD Bioscience) and 200 μg/mL Rat Gamma Globulin (Jackson ImmunoResearch) ...
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bioRxiv - Immunology 2019Quote: ... 0.5-1×106 cells were incubated for 30 minutes at 37°C with antibody cocktails composed of 100µl of Stain Buffer (FBS) (BD Biosciences), 10µl of BD HorizonTM Brilliant Stain Buffer Plus (BSB ...
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bioRxiv - Cell Biology 2020Quote: For HSC isolation the cells were stained with the following antibody cocktail: PECy5 conjugated anti-lineage specific antibodies: CD2 (BD), CD3 (BD) ...
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bioRxiv - Immunology 2020Quote: ... for 5 min at RT prior to staining with a surface stain cocktail containing the following antibodies purchased from BD and Biolegend ...
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bioRxiv - Immunology 2020Quote: Cells were incubated in 50 μL of FACS buffer containing a cocktail of antibodies for 30 minutes on ice prior to washing and analysis on a FACS Aria (BD). Antibodies included anti-IgM FITC (G20-127 ...
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bioRxiv - Immunology 2021Quote: ... normal mouse and normal rat serum followed by incubation with pre-titrated immunoglobulin specific antibody cocktails prepared in brilliant stain buffer (BD) for 30 min on ice ...
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bioRxiv - Immunology 2021Quote: ... and incubated in surface-stain cocktail consisting of 50µL BD Horizon Brilliant Violet Stain Buffer (BD Bioscience, Franklin Lakes, NJ) plus antibodies (see Supplementary Tables 2-4 ...
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bioRxiv - Immunology 2021Quote: ... Surface antibody cocktail was prepared in cell staining buffer (Fluidigm) and added to all samples equally after Fc block (BD). Cells were washed then fixed and permeabilized using eBioscience Transcription Factor Fix/Perm Buffer followed by barcoding (Fluidigm) ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed twice (900 x g for 5 minutes at room temperature) and then 50µL of surface antibody cocktail diluted in Brilliant Violet buffer (BD Biosciences) added and incubated 20 minutes at room temperature in the dark ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were stained using a mouse anti-human antibody cocktail to cell surface markers: CD3-BV711 (BD Biosciences, clone UCHT1), CD14-BV711 (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... and lymph nodes of mice vaccinated for tumor challenge experiments, cells were incubated with 100 μL of antibody cocktail (surface antibodies, CD16/CD32 (BD), and fixable viability dye (eBiosciences ...
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bioRxiv - Neuroscience 2024Quote: ... 50 µl of cell suspension was stained using an antibody cocktail (Table 1) diluted in Brilliant Stain Buffer (BD, U.S.). Cells were incubated with antibodies for 30 min at 4 °C in dark followed by washing in sterile PBS (pH 7.2) ...
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bioRxiv - Developmental Biology 2023Quote: ... The following antibodies were used to stain for HSCs: FITC-conjugated lineage cocktail with antibodies anti-CD4 (clone H129.19, BD Pharmingen), CD3e (clone 145-2C11 ...
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bioRxiv - Immunology 2023Quote: ... PBMCs were stained using a mouse anti-human antibody cocktail to cell surface markers: CD3-BV711 (BD Biosciences, clone UCHT1), CD14-BV711 (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... The following antibodies made up the extracellular cocktail: CD3-BUV395 (145-2C11, RRID: AB_27382, BD Biosciences, Franklin Lakes, NJ, USA), CD4-AF700 (RM4-5 ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were then blocked with anti-mouse CD16/32 and labeled with cell surface antibody cocktail including anti-mouse CD3-FITC、anti-mouse CD4-APC and anti-mouse CD8-Percp-cy5.5 (BD Bioscience) for 30min at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Single cells from digested xenograft tumors were labeled using a BUV395 anti-mouse cocktail (pooled and diluted 1:40: anti-mouse H2kD [BD Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: ... Single cells from digested xenograft tumors were labeled using a BUV395 anti-mouse cocktail (pooled and diluted 1:40: anti-mouse H2kD [BD Biosciences, cat. 742437], anti-mouse CD45 [BD Biosciences ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 1.5 mL of each milt sample was diluted 1:9 in Storfish (IMV Technologies, France; an inactivating medium) in 15 mL tubes (Falcon, BD Biosciences, Allschwil, Switzerland). These tubes were then placed on ice and are referred to as ‘diluted milt’.
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bioRxiv - Neuroscience 2022Quote: Whole blood was collected from non-fasted and overnight fasted (16 hr) mice in an EDTA-treated tube (cat# 365974; BD, Franklin Lakes, NJ) by cardiac puncture with a 23-25G needle from a deeply anesthetized animal ...
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bioRxiv - Developmental Biology 2022Quote: ... Blood samples were collected from the jugular or coccygeal vein (according to the size of the animal) using EDTA tube (BD vacutainer, BD, Spain) and stored at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... After 48h single colonies were picked and inoculated into 15ml falcon tubes containing 3ml nutrient broth (5g/L peptone BD difco, BD Bioscience; 3g/L yeast extract BD difco, BD Bioscience), and were grown overnight at 28°C shaken at 250rpm ...
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bioRxiv - Plant Biology 2021Quote: ... special order cytometer with a 514-nm laser exciting fluorescence that is cut off at 525 nm prior to photomultiplier tube collection (BD, Franklin Lakes, NJ). Events were annotated ...
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bioRxiv - Molecular Biology 2019Quote: ... A further 8 mL of blood was collected in tubes containing Clot activator and gel for serum separation (SST II Plus, BD Vacutainer®, USA), and another 3 mL of blood was collected in tubes containing sodium heparin (Sodium HeparinN Plus ...
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bioRxiv - Biochemistry 2021Quote: ... Blood was collected by cardiac puncture (400-600 μl) in BD Microtainer Blood Collection tubes with clot activator gel containing heparin (BD, Franklin Lakes, NJ). Blood samples were collected by centrifugation and sent to the University of Michigan In-Vivo Animal Core (IVAC ...
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bioRxiv - Immunology 2021Quote: Absolute numbers of B cells and T cells in peripheral blood were measured using BD Multitest™ 6-color TBNK reagents and BD Trucount™ Tubes (BD Biosciences) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... were resuspended in defRPMI-1640 with 3% newborn calf serum and Benzonase™ (FACS buffer) in 5 mL FACS tubes and pre-incubated with GolgiStop™ (BD Biosciences) for ∼60 min at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... followed by inoculation in Mycobacteria Growth Indicator Tubes (MGITs) incubated in a BD BACTEC TM MGIT TM 960 instrument (BD, Sparks, MD, USA). Extrapulmonary samples were inoculated using the same medium without prior decontamination ...
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bioRxiv - Molecular Biology 2022Quote: Blood samples were also collected from the same rabbits into serum separator tubes for checking their health status (creatinine, albumin, cholesterol and triglycerides, BD Vacutainer, ref: 367953).
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bioRxiv - Immunology 2022Quote: ... 8 mLs of peripheral blood was drawn into each of two BD Vacutainer Cell Preparation Tubes with sodium citrate (BD Biosciences catalog #362760). The tubes were centrifuged at 1,800 x g for 30 minutes at room temperature (RT) ...