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Citations for Becton, Dickinson and Company :
1301 - 1350 of 2032 citations for CD300A Human HEK293 Fc His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... 1 μg of purified rat anti-mouse CD16/CD32 was added to each pool (Mouse BD Fc Block, BD Biosciences, cat. 55142) and incubated for 30 minutes at 4°C ...
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bioRxiv - Genetics 2022Quote: ... and ICAM1-Fc (a gift from Professor Alister Craig, Liverpool School of Tropical Medicine) spotted on bacteriological petri dishes (BD Falcon 351007) at a concentration of 50μg/ml ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The cells were washed twice with FC buffer by centrifugation (1000 rpm/5minutes) and incubated with streptavidin−PE conjugate (BD bioscience, 349023) for 30 minutes at 4ºC ...
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bioRxiv - Cell Biology 2024Quote: ... cells were counted using a hemacytometer and then treated with an Fc blocking buffer containing anti-Cd16/32 (BD Biosciences, 1:500) for 20 minutes at 4 degrees Celsius before a 30 minute incubation with antibody master mixes for the myeloid or lymphoid panels at 4 degrees Celsius ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed with FACS staining buffer (PBS supplemented with 2% fetal bovine serum and 1 mM EDTA) and incubated with Fc receptor blocker purified rat anti-mouse anti-CD16/CD32 (BD Biosciences, 553142) for 20 min at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... The cells were then washed with flow cytometry buffer [PBS + 0.5% Bovine Serum Albumin (BSA) (Fisher BioReagents, BP9706100) + 2 mM EDTA] and incubated at 4°C with Fc Block (BD Biosciences, 553142) for 10 min ...
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bioRxiv - Cell Biology 2022Quote: ... labelled with cell hashing antibodies and isolated by FACS (all steps at 4°C): Cell suspensions were incubated with Fc-block (1:50, BD Pharmingen 553142) for 10min and subsequently incubated with mouse CD45 MicroBeads (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: ... all cells were harvested from the culture plates and the cell Fc receptors were blocked for 10 min at 4°C with 10 μg/mL anti-CD16/CD32 antibodies (BD Biosciences 553142). Then ...
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bioRxiv - Cancer Biology 2023Quote: ... 5X106 cells per mL were washed with cold PBS and blocked in PBS with Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™, BD Biosciences) for 15 min at 4°C in the dark ...
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bioRxiv - Bioengineering 2023Quote: ... resuspended in 100 μL of PBS containing 5% FBS and blocked with 2.5 μg of Fc-blocking solution (BD Bioscience, Cat #564220) for 15 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Eluent was washed twice, incubated with DR (Bio-Legend, 424101, 1:1000) and CD16/CD32 Fc block (BD Biosciences, 553141, 1:200) at room temperature for 15 minutes ...
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bioRxiv - Immunology 2023Quote: Splenocytes-or BM-derived cells were resuspended in sPBS and the Fc receptors were blocked for 10 min at 4°C with 10 μg/mL anti-CD16/CD32 antibodies (BD Biosciences 553142). After blocking ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were incubated with anti-mouse CD32 for 5 min at 4°C to block Fc receptors and stained with fluorochrome conjugated antibodies to CD45R/B220 PE (1:200) (clone RA3-6B2, BD Bioscience #553089), Ter119 PE (1:200 ...
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bioRxiv - Immunology 2023Quote: ... and media was tested by ELISA according to the manufacturer’s instructions (BD OptEIA Human IL-10 ELISA set #3555157 and BD OptEIA Human IL-1B ELISA set 2 #557953).
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bioRxiv - Microbiology 2021Quote: ... the cryosections were incubated for 1 h in the blocking solution (PBS containing 5% goat serum and 5 μg/mL mouse BD Fc Block™) and then labeled overnight at 4°C with the APC-conjugated rat anti-mouse CD45 antibody (1:100 dilution ...
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bioRxiv - Immunology 2020Quote: ... The cells were then washed and resuspended in PBS supplemented with 2% FCS for FACS analysis (FACSCanto II; BD Bioscience, San Jose, USA). FACS data analysis was performed using BD FACSDivaTM Software 8 (BD Biosciences ...
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bioRxiv - Cancer Biology 2020Quote: ... Unspecific binding was prevented using the ells were stained using the BD Pharmingen Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) (Clone 2.4G2 ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated in the FC wash buffer (2h at 4 °C) containing FITC-coupled anti-CD44 antibody (1 to 80 BD Pharmingen Cat.No. 555478) or its isotype control (FITC Mouse IgG2b ...
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bioRxiv - Immunology 2021Quote: We permeabilized formalin-fixed glomerular and tubulointerstitial single cells with 0.25% Tween-20 in PEB buffer at room temperature for 30 minutes and blocked cells with Fc Block (clone 2.4G2, BD Biosciences, San Jose, CA) diluted in 0.25% Tween-20 on ice for 10 minutes ...
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Multiplexed single-cell transcriptomic analysis of normal and impaired lung development in the mousebioRxiv - Cell Biology 2019Quote: ... Cells were incubated in the dark with 2 μl/1×106 cells of CD16/32 antibody (Fc block; BD Biosciences, Mississauga, ON, Canada) for 15 minutes at RT ...
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bioRxiv - Physiology 2021Quote: ... Following resuspension in PBS and passage through a 70μm strainer, cells were incubated in Fc block solution (Bio Rad, Cat# BUF041A) and PerCP-conjugated anti-CD11b antibody (BD Pharminogen, Cat# 550993). After PBS washes ...
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bioRxiv - Immunology 2021Quote: ... samples were stained with the relevant combination of surface and intracellular antibodies in combination with Fc blocking antibody CD16/CD32 (BD Pharmingen, Cat. #553141). BV421 anti-MHCII(IA/IE ...
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bioRxiv - Immunology 2021Quote: ... cell pellets were resuspended in x μL of unlabeled CD16/32 mAb Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™), BD Pharmingen 553141) ...
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Regulatory T cells control type 1-driven immunopathology restraining GM-CSF-producing helper T cellsbioRxiv - Immunology 2024Quote: ... cells were restimulated ex vivo for 4-5h at 37 °C in complete RPMI medium (RPMI supplemented with 2mM L-Glutamine and 10% fetal calf serum (FCS)) with GolgiPlug (BD, 1:1,000 dilution), phorbol 12-myristate 13-acetate (50ng/ml ...
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bioRxiv - Immunology 2022Quote: ... blood cells were stained in 50 µl 2% FCS RPMI medium with antibodies against CD11C (APC, clone HC3, BD biosciences, dilution 1/100), CD11b (PercPCy5.5 or Pacific Blue ...
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bioRxiv - Immunology 2023Quote: ... cells were washed 2x in FACS buffer (5% FBS, 2 mM EDTA in PBS) and resuspended with anti-CD16/CD32 Mouse BD FC Block (BD Biosciences, Cat. 553142) in FACS buffer at a concentration of 10 ug/ml for 15 min ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then incubated at 4°C for 15 min with Fc Block (Rat anti-mouse CD16 and CD32; BD Pharmingen – Cat.553141) and 30 min with the antibody mixes ...
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bioRxiv - Immunology 2023Quote: Cells were first labelled with Fc block for 10 minutes at 4°C and then the following antibodies added: CD45-PE (BD, 30-F.11), Siglec F-APC (BioLegend ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell suspensions were blocked for 5 min at 4 °C with rat anti-mouse CD16/CD32 (#553142, Mouse BD Fc Block, BD Biosciences) in FACS buffer ...
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bioRxiv - Physiology 2024Quote: ... then cells were washed with 1x PBS and resuspended in FACS buffer and non-specific binding to Fc receptors was blocked by incubation with anti-CD16/32 antibody (BD Pharmingen, Cat# 553141) and the FACS antibodies BV711 conjugated anti-CD11b and Pacific blue conjugated anti-CD45 antibodies (Cat# 101241 ...
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bioRxiv - Cancer Biology 2021Quote: ... then 10 mL of anti-human CD11b/Mac-1 (555388, BD Bioscience) solution (25% in blocking buffer ...
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bioRxiv - Cell Biology 2019Quote: ... mouse anti-Oct3/4 human isoform A (BD Biosciences, 561628, 1:40) and rabbit anti-Phospho-Histone H2A.X (CST ...
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bioRxiv - Immunology 2020Quote: ... FITC mouse anti-human CD40 (1:20 dilution, Clone 5C3, BD Pharmingen), Brilliant Violet 421™ mouse anti-human CD69 (1:40 dilution ...
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bioRxiv - Synthetic Biology 2022Quote: ... AF647 Mouse anti-human Akt pS473 clone M89-61 (BD Biosciences #560343).
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bioRxiv - Neuroscience 2021Quote: Human microglia cell line was plated in T150 flasks (BD Falcon 355001) at 20 million/flask in 25mL complete microglia media and placed in 37C 5% CO2 incubator ...
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bioRxiv - Immunology 2022Quote: ... and 25 ng/mL mouse anti-human CD3 antibody (UCHT1; BD Bioscience). T cells were incubated at 37°C for 4-5 weeks and fed biweekly with R10-Max and 50 U/mL IL-2 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD45 BUV395 (1:100, clone HI30, BD Biosciences, cat # 563792), Anti-human CD16 BUV395 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD3 BUV395 (1:100, clone UCHT1, BD Biosciences, cat # 563546), Anti-human CD69 BUV496 (1:50 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD16 BUV395 (1:100, clone 3G8, BD Biosciences, cat # 563785), Anti-human CD3 BUV395 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD15 PE (1:100, clone W6D3, BD Biosciences, cat # 562371), Anti-human CD163 PE (1:50 ...
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bioRxiv - Immunology 2021Quote: ... and kappa light chain (PE anti-human kappa light chain; BD Biosciences) double positivity using a SH800S Cell Sorter (Sony Biotechnology) ...
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bioRxiv - Physiology 2019Quote: ... the monoclonal antibody PE mouse anti-human CD105 (Ref. 560839, BD Biosciences) was used ...
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bioRxiv - Immunology 2019Quote: ... and stained for 30 minutes with anti-human CD4-BUV395 (BD Biosciences), CD25-PE-Dazzle594 (Biolegend) ...
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bioRxiv - Immunology 2021Quote: ... Plates were coated with mouse anti-human IFN-γ antibody (BD Pharmigen) at 5 μg/well and incubated at 4°C overnight ...
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bioRxiv - Immunology 2021Quote: ... followed by a mixture of the following anti-human antibodies from BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... 5 μL mouse anti-human CD16-APC-H7 (BD® cat. 560195), 5 μL mouse anti-human CD19-V450 (BD® cat ...
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bioRxiv - Immunology 2021Quote: ... cells were stained with α-human CD8-FITC (G42-8, BD Pharmingen), CD69-BV605 (FN50 ...
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bioRxiv - Bioengineering 2020Quote: ... and ITS+ Premix (1%) and human EGF (20 ng/ml) from BD Biosciences.
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bioRxiv - Systems Biology 2021Quote: Gardnerella vaginalis isolate 11E4 were maintained on Human Bilayer Tween Agar (BD) plates and NYC III medium according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: The human inflammatory cytometric bead array (CBA) kit was purchased from BD Biosciences (San Jose ...