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Citations for Becton, Dickinson and Company :
1251 - 1300 of 1653 citations for Recombinant Human SPESP1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... in the presence of 100 U/ml of human IL-2 and soluble anti-CD28 antibody (BD Biosciences, Clone: 37.51). T lymphoblasts were cultured at 37 °C in 5% CO2 for 3 days in exponential growth phase ...
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bioRxiv - Immunology 2024Quote: Blood samples from healthy volunteers to obtain ABO-matched normal human serum were collected in vacutainer tubes for serum analysis (ref. no. 367896, BD).
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bioRxiv - Cancer Biology 2021Quote: ... Band densitometry conducted using Image J relative to housekeeping protein Actin (1:10,000, BD Transduction laboratories ...
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bioRxiv - Cell Biology 2020Quote: ... the primary antibody anti-GM130 (Golgi matrix protein 130) (1:10, BD Pharmingen, USA) was incubated overnight at 4°C ...
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Molecular coevolution of nuclear and nucleolar localization signals inside basic domain of HIV-1 TatbioRxiv - Evolutionary Biology 2021Quote: ... and cells expressing target proteins were selected using a FACSAria III cell sorter (BD). EGFP-Tat and EGFP were immunoprecipitated from U2OS cells using 25 μl of GFP-Trap magnetic beads (ChromoTek GmbH ...
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bioRxiv - Neuroscience 2020Quote: ... Soluble proteins were incubated overnight with 2.5 μg of anti-GluN1 antibody (BD Pharmingen). Immune complexes were isolated by the addition of 20 μL of protein G– Sepharose beads (GE Healthcare ...
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bioRxiv - Neuroscience 2019Quote: ... Mouse monoclonal antibodies were: anti-Golgi matrix protein of 130 kDa (GM130) (610822, BD Biosciences ...
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bioRxiv - Neuroscience 2019Quote: ... Mouse monoclonal antibodies were: anti-Golgi matrix protein of 130 kDa (GM130) (610822, BD Biosciences ...
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bioRxiv - Cancer Biology 2020Quote: Meiotic and kinetochore genes tagged with Enhanced Green Fluorescent Protein (EGFP) (from BD Biosciences) were used ...
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bioRxiv - Immunology 2022Quote: ... and BD GolgiPlug Protein Transport Inhibitor (containing brefeldin A; 1 mg/mL; BD Biosciences). Cells were washed ...
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bioRxiv - Immunology 2021Quote: ... and cells were stained for interferon-γ and macrophage inflammatory protein-1β (BD bioscience). Flow cytometry was performed with an IQue (Intellicyt ...
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bioRxiv - Immunology 2020Quote: ... Then, GolgiPlug and GolgiStop (brefeldin A and monensin, respectively) protein transport inhibitors (BD Biosciences) were added ...
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bioRxiv - Immunology 2021Quote: ... The protein transport inhibitor monensin (GolgiStop, Cat#51-2092KZ, BD Biosciences, San Jose, CA) was added to the wells to prevent cytokine secretion 60 minutes after the addition of the peptides ...
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bioRxiv - Microbiology 2020Quote: ... Proteins of interest were detected using primary polyclonal anti-FliK and anti-FliC (BD) antibodies as well as monoclonal anti-DnaK (abcam ...
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bioRxiv - Immunology 2021Quote: ... Protein concentration was normalized through the Bradford assay (BD Biosciences, San Diego, CA, USA). The absorbance was taken using Multiskan Go (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... and blotted for antibodies against specific proteins HIF1α (BD biosciences, cat# 610958, 1:1000), HK-2 (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2022Quote: ... anti-C-terminal binding protein 2 (mouse anti-CtBP2 IgG1; BD Biosciences; 1:200), and anti-glutamate receptor 2 (mouse anti-GluR2 IgG2a ...
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bioRxiv - Immunology 2023Quote: ... The green fluorescent protein (GFP) signal from cells was measured using a FACSVerse (BD). To measure autophagic flux ...
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bioRxiv - Biochemistry 2023Quote: ... cells expressing red fluorescent protein were enriched by FACS Aria Fusion SORP (BD Bioscience) and plated into a dish at a very low density ...
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bioRxiv - Systems Biology 2023Quote: ... All of the other data considered here (MERFISH, BD Rhapsody mRNA and protein, etc.) ultimately consists of a similar matrix ...
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bioRxiv - Cancer Biology 2023Quote: CD81 cell surface protein expression was determined by FCM (LSR Fortessa X20, BD Biosciences), 5 x105 cells were labeled with 1 μg anti-hCD81-PE clone 5A6 or 1 µg isotype control-PE (349506 ...
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bioRxiv - Immunology 2023Quote: ... CD3 peridinin chlorophyll protein (PerCP) Cy5.5 clone SP34-2 (BD Biosciences Cat No. 552852), IgD phycoerythrin (PE ...
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bioRxiv - Systems Biology 2020Quote: ... White cells were resuspended in PBS supplemented with 1% human serum and 2 mM EDTA and analyzed on an LSR Fortessa instrument (BD Biosciences).
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bioRxiv - Immunology 2021Quote: Supernatants were collected 4 or 24 h post-challenge and the levels of TNF and mature IL-1β were quantified by ELISA using OptEIA human TNF ELISA Set (BD Biosciences) and human IL-1β/IL-1F2 DuoSet ELISA (R&D systems) ...
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bioRxiv - Immunology 2021Quote: CD47 expression or cross-dressing on cells was determined by direct staining with BV786-conjugated anti human specific CD47 mAb B6H12 (abbreviated anti-hCD47; BD Bioscience) or PE-conjugated anti-human CD47 mAb CC2C6 (with cross-reactivity to pig CD47 and thus ...
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bioRxiv - Microbiology 2020Quote: Macrophages were infected in a 48-well plate as described above and harvested supernatants were assayed for cytokine levels using ELISA kits for human IL-1β (BD Biosciences) and IL-18 (R&D Systems).
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bioRxiv - Molecular Biology 2021Quote: ... Cells were re-suspended in 100 μl of FCSB and 1 μl of mouse anti-human CD10-PE (561002, BD Biosciences) and mouse anti-human CD92-AF647 (565316 ...
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bioRxiv - Neuroscience 2022Quote: Dissociated and resuspended iTF-Microglia were blocked for 15 min with 1:20 Human FC Block (BD Biosciences; Cat. No. 564220) and then stained with 1:66 PE/Cy7 anti-human CD184 (CXCR4 ...
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bioRxiv - Immunology 2022Quote: ... NK cells were added to the washed ELISA plate and incubated together with anti-human CD107a (BD, 1:40, clone: H4A3), brefeldin A (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... primary hMDMs were transfected in a 48-well plate as described above and harvested supernatants were assayed for cytokine levels using an ELISA kit for human IL-1β (BD Biosciences) according to the manufacturer’s instructions.
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bioRxiv - Bioengineering 2019Quote: ... with anti-positive (CD90, CD105, CD44) and negative marker (CD34, CD11b, CD19, CD45, MHC class II) antibodies (Human MSC Analysis Kit, 562245, BD Biosciences), as described in detail in [42] ...
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bioRxiv - Developmental Biology 2019Quote: ... Cell suspensions from APEL vascular differentiations were incubated with mouse anti-human CD31-APC (eBioscience, San Diego, CA) and CD146-PE (BD Biosciences) antibodies for 30 min on ice ...
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bioRxiv - Molecular Biology 2019Quote: ... The HLA-DQ cell surface expression was performed by cytofluorimetric analysis using the FACSAria III and DIVA software with FITC mouse anti-human HLA-DQ antibody (BD Biosciences). The quantitation of specific transcripts was performed by qRT-PCR as described in [9] ...
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bioRxiv - Cancer Biology 2019Quote: ... cKIT and PDGFRA expression was assessed using mouse anti-human CD117 (PE-Cyanine7, clone 104D2, #25-1178-42, eBioscience) and CD140a (PE, clone αR1, #556002, BD Pharmingen) respectively ...
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bioRxiv - Cancer Biology 2019Quote: ... Membranes were blocked for 1 h in 5% milk/TBS Tween-20 solution and incubated for 1 h with anti-TOP2β (1:5,000, mouse anti-human, Clone 40/Topo IIβ, RUO – 611492, BD Biosciences-US) or anti-TOP2α (1:10,000 ...
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bioRxiv - Microbiology 2019Quote: ... Cells were then washed three times in PBS followed by incubation in 1% Human Fc Block (BD Biosciences, San Jose, CA) in PBS ...
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bioRxiv - Cell Biology 2019Quote: ... cells were stained with a 1:50 dilution of phycoerythrin (PE)-conjugated mouse monoclonal antibody against human TRAIL (550516, BD Biosciences). For immunophenotyping of MSCs the cells were stained with 1:50 dilution of fluorochrome-conjugated mouse monoclonal antibodies against CD73 (PE-Vio770 ...
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bioRxiv - Bioengineering 2020Quote: ... Cytokine concentrations in the culture supernatant were measured using BD Cytometric Bead Array Human Th1/Th2 Cytokine Kit II (BD Biosciences).
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bioRxiv - Immunology 2021Quote: ... Cytokines in the serum sample were measured using human Th1/Th2/Th17 cytometric bead array kit (BD Bioscience, San Jose, CA) according to the manufacture’s instruction ...
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bioRxiv - Immunology 2021Quote: ... labelled with anti-human myeloid and stromal markers (Table 1) and analysed by flow cytometry on a LSR Fortessa (BD Biosciences).
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bioRxiv - Immunology 2020Quote: ... the PBMCs were stimulated with pooled or individual peptides at a final concentration of 10 μg/mL per individual peptide for 1 h in the presence of 2 μg/mL monoclonal antibodies against human CD28 (BD Pharmingen) and CD49d (BD Pharmingen ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were then washed twice 5 min in Tris buffer saline containing 0.01% Triton X-100 (TBST) and incubated with the primary antibody over night at 4°C (human and mouse a-Syn: 610786 BD Biosciences). After incubation with the first antibody ...
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bioRxiv - Neuroscience 2020Quote: ... Human CD34C-derived ECs were seeded at a density of 8×104/insert onto the Matrigel-coated (BD Biosciences, United States) Transwell inserts (3401-Costar ...
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bioRxiv - Cancer Biology 2019Quote: ... and human prostate cancer propagating cells were resuspended in 120 µl of a 1:1 mixture of Matrigel (BD Biosciences, #354234) and PrEGM (Lonza ...
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bioRxiv - Microbiology 2020Quote: ... adjusted to 106 CFU ml-1 in HBSS and 104 CFU (10 μl) used to inoculate 90 μl of freshly donated human blood (collected in EDTA-treated tubes; BD Biosciences) or freshly diluted H2O2 (10 mM in PBS ...
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bioRxiv - Microbiology 2020Quote: ... IL-10 and IL-12p70 were quantified in those culture supernatants by ELISA with BD OptEIA™ human IL-10 and IL-12p70 sets following the manufacturer's instructions (BD Bioscience).
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bioRxiv - Genomics 2021Quote: ... hPBMCs were resuspended in 1x PBS + 2% FBS and stained with a FITC-conjugated Mouse Anti-Human CD45 Antibody (Clone HI30, BD Biosciences) for 25 min on ice ...
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bioRxiv - Immunology 2020Quote: ... 8.0 × 105 cells per condition were fixed in 1% PFA for 10 min at room temperature and subsequently stained with primary antibodies: mouse anti-human PEcy7-CD16 (BD #557744), mouse anti-human PerCPcy5.5-CD66b (Biolegend # 305107) ...
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bioRxiv - Microbiology 2021Quote: ... Intracellular staining of the cytokines was performed after fixation and permeabilization using APC conjugated anti-human IFNγ (BD Bioscience, Cat# 551385), anti-human IL2 (BD Bioscience ...
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bioRxiv - Cancer Biology 2021Quote: ... Flow cytometry for negative checkpoint regulator was performed as previously described(14) using antibodies: BV510 mouse anti-human CD279 (PD1) (1:100; 563076, BD Horizon), PerCP-eFluor 710 mouse anti-human CD223 (LAG-3 ...