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Citations for Becton, Dickinson and Company :
1251 - 1300 of 1404 citations for Recombinant Human NCR3 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: PBMC were labelled for FACS staining with mouse anti-human antibodies directed against lineage markers (Vioblue-CD3, CD14, CD19, CD56 and CD11c) (BD Biosciences), APC-Vio770 HLA-DR ...
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bioRxiv - Immunology 2020Quote: ... followed by staining for 3 days at 37 °C with 1:100 dilutions of BV421-labeled mouse anti-human CD35 clone E11 (BD Biosciences) and Alexa Fluor 488-labeled mouse anti-Ki67 clone B56 (BD Biosciences ...
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bioRxiv - Biochemistry 2020Quote: ... to stain for CD69 receptor using 5 µL (100 µg/mL) of PreCP-Cy ™ 5.5 mouse anti-human CD69 (BD Biosciences). Staining was done for 45 mins on ice then washed one time with 2 mL RPMI-1640 medium and reconstituted in 250 µL RPMI-1640 medium ...
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bioRxiv - Neuroscience 2022Quote: ... Syn1 [a mouse monoclonal antibody that recognizes residues 91-99 from the NAC region of human α-synuclein (51)] (BD Biosciences) at 1:4,000 and PER4 at 1:4,000 ...
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bioRxiv - Immunology 2022Quote: ... The membranes were then incubated with human mAbs (at a concentration of 1 µg/ml) and mouse anti-Hisx6 antibody (1 µg/ml, BD Biosciences) in PBS-T 5% dry milk for 2 h ...
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bioRxiv - Immunology 2022Quote: ... and then incubated with human mAbs (at a concentration of 1 µg/ml) and mouse anti-Hisx6 antibody (1 µg/ml, BD Biosciences) in PBS-T 5% dry milk in each channel for 2 h ...
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bioRxiv - Immunology 2022Quote: ... whereas 100 µl PBS+/+ containing 20 ng/ml recombinant IL-1β supplemented with 100 µg/ml PTX3 and/or 100 µg/ml mouse anti-human CD62P (clone AK-4, BD Bioscience) was added to the apical side (upper chamber) ...
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bioRxiv - Cancer Biology 2022Quote: ... For DAC studies on human MPNST xenograft bearing CB-17 SCID mice (Taconic): all cells were implanted into mice 1:1 with Matrigel (BD Biosciences). M3 cells (stably infected with MSCV-Luciferase-PGK-Hygro retrovirus ...
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bioRxiv - Neuroscience 2019Quote: ... Supernatant was aspirated and the cell pellet was resuspended in 200uL of FACS buffer (PBS, 0.5% BSA, 2mM EDTA, 20mM Glucose) with 1:50 PE Rat Anti-Human CD49f antibody (BD Biosciences; 555736) and incubated on ice for 20 minutes ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... IL-12 and IL-6 was simultaneously analyzed by flow cytometry (FACS Canto II; Becton-Dickinson) using the BD Cytometric Bead Array (CBA) Human Inflammatory Cytokines Kit (BD™) according recommendations ...
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bioRxiv - Genetics 2020Quote: Human embryonic stem (hES) H1 cells (WA01, WiCell) were maintained under feeder free conditions on Matrigel-coated plates (#354277, BD Biosiences) in mTeSR™1 ...
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bioRxiv - Developmental Biology 2019Quote: Endothelial network assembly was assayed by the formation of capillary-like structures by Human Uterine Microvascular Endothelial Cells (HUtMEC) on Matrigel (BD Biosciences). Matrigel was diluted 1:1 with a supplement-free M200 medium ...
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bioRxiv - Immunology 2020Quote: ... Cells with SARS-CoV-2 peptides and positive control were co-stimulated with CD28/CD49d at a final concentration of 5 μg/ml (anti-human CD28/CD49d Purified Clone L293 L25 BD Biosciences). Basal IFN-γ production by PBMCs was included as background control in the absence of stimulation and co-stimulation ...
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bioRxiv - Cancer Biology 2019Quote: ... and antibody to Fc receptor binding inhibitor (eBioscience) before incubation with the following primary antibodies: PE-conjugated anti-human CD31 (BD Pharmingen), PE-conjugated anti-human CD45 (BD Pharmingen) ...
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bioRxiv - Immunology 2019Quote: ... Activation assays were performed by culturing cells with recombinant human IL-2 (10ng/ml, RND Systems) in the presence of coated anti-CD3 and anti-CD28 (2mg/ml, BD Biosciences) in the culture medium ...
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bioRxiv - Immunology 2021Quote: Interleukin-8 secretion of treated THP-1 monocytes was quantified by the ELISA OptEIA™ Set for human interleukin-8 (BD) following the manufacturer’s protocol with optimized wash buffer composition (50x dilution) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... activation of T-cells was measured by flow cytometry using the anti-human CD4 (FITC)/CD69 (PE)/CD3 (PerCP) antibody staining solution (BD Biosciences) in combination with anti CD25 (APC ...
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Enhancer Remodeling Promotes Tumor-Initiating Activity in NRF2-Activated Non-Small Cell Lung CancersbioRxiv - Cancer Biology 2020Quote: ... and human tumor cells were collected as the mouse MCH class I-negative fraction using flow cytometry (BD FACSAria, Becton Dickinson). Then ...
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bioRxiv - Pathology 2021Quote: Cells were fixed in 2% PFA for 15 minutes at room temperature and incubated with mouse anti-human VE-Cadherin (BD Sciences) at 4°C overnight as previously described 36 ...
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bioRxiv - Immunology 2020Quote: ... samples were first pre-incubated with human BD Fc Block to block non-specific binding (BD Pharmingen, Franklin Lakes, NJ, USA) for 20 minutes at room temperature ...
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bioRxiv - Immunology 2021Quote: Cells were co-cultured in R10 medium alone or in presence of K562 cells for 6 hours in the presence of anti-human CD107a (H4A3, FITC, BD Biosciences). Golgi plug and Golgi stop (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: Supernatants were collected after 4 or 24 h and the levels of TNF and IL-1β were quantified by ELISA using OptEIA human TNF ELISA Set (BD Biosciences) and human IL-1β/IL-1F2 DuoSet ELISA (R&D systems ...
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bioRxiv - Immunology 2021Quote: ... or with no target cells (or an E:T of 1:0) and cultured overnight before being staining with allophycocyanin (APC)-conjugated anti human-CD69 antibody (BD Biosciences #555533). Flow cytometry was performed using a BD- Fortessa (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... T cells from different human donors (P1, P2, P3) were isolated using the RosetteSep™ negative isolation method and activated (using BD Human T cell activator beads coated with anti-CD3 and anti-CD28 ...
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bioRxiv - Immunology 2020Quote: Cells were washed in PBS and resuspended in PBS containing 2% fetal calf serum and 2 mM EDTA and stained with the extracellular mix using anti-human CD14-BV421 antibody (clone M5E2, BD Bioscience) and anti-human CD36-BUV605 antibody (clone CD38 ...
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bioRxiv - Immunology 2021Quote: ... To this end 1×104 targets per well and 2×104 effector cells per well were each preincubated for 2 h in 10 μg/ml anti-human CD178 antibodies (NOK-1 and NOK-2, BD Biosciences) or 5 μg/ml anti-human TRAIL antibody (RIK-2 ...
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bioRxiv - Genomics 2020Quote: Immune cells for bulk mRNA sequencing were incubated with Fc Block for 20 minutes and then stained with one of six panels of directly conjugated antibodies for 30 minutes: anti-human CD16 (BD 558122), CD123 (BD 560826) ...
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bioRxiv - Immunology 2019Quote: ... cDNA was amplified - 10 cycles for resting cells and 9 cycles for in vitro activated cells - using the Human Immune Response primer panel (BD Biosciences), containing 399 primer pairs ...
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bioRxiv - Immunology 2021Quote: ... anti-mouse IFN-γ ELISA set (Cat no. – 555138) and anti-human IL-10 ELISA set (Cat no. – 555157) were purchased from BD Biosciences ...
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bioRxiv - Genomics 2021Quote: Healthy human hiPSC cell lines for Figure 4 were cultured under feeder-free conditions on plates coated with human-qualified Matrigel (BD Biosciences) diluted 1:40 in mTeSR-1 medium (StemCell Technologies) ...
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bioRxiv - Immunology 2022Quote: ... Mouse and rat anti-human monoclonal antibodies (mAbs) used in this study included: IFNγ FITC (clone 25723.11, BD Biosciences, cat# 340449), TCRαβ Percp 710 (clone IP26 ...
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bioRxiv - Cancer Biology 2022Quote: The fraction of CD44+/CD24− cells was determined by trypsinizing and resuspending cells in FACS buffer at 10×106 cells/mL followed by incubation with antibodies against human CD44 (APC-conjugated, Clone G44-26, 1:5, BD Biosciences) and CD24 (PE-Cy7-conjugated ...
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bioRxiv - Cancer Biology 2022Quote: ... and engraftment of human cells in the bone marrow and spleen was measured with flow cytometry (CD45-PE-Cy7 antibody, BD Biosciences). Single-cell suspensions were prepared from the bone marrow ...
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bioRxiv - Cancer Biology 2022Quote: ... Isolation of CD4+ T cells was carried out by negative selection using the Human CD4 T Lymphocyte Enrichment Set-DM (BD Biosciences). All patient samples were obtained with informed consent for research use ...
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bioRxiv - Cancer Biology 2022Quote: ... Primary AML cells were harvested and stained with human CD45 and cells were analyzed by a high-throughput automated plate reader (BD LSRFortessa). Dead cells were excluded using the Near-IR Live/Dead marker (Invitrogen) ...
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bioRxiv - Immunology 2022Quote: Human and Non-human primate samples were processed similarly: Blood was collected in 8 mL sodium citrate-containing CPT tubes (BD Biosciences) and separated into plasma and peripheral blood mononuclear cells (PBMC ...
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bioRxiv - Neuroscience 2022Quote: ... and tumor necrosis factor alpha (TNFα) in cell supernatants were measured using the Human Inflammatory Cytokine Cytometric Bead Array Kit (BD Biosciences). A LEGENDplex™ HSC Myeloid Panel kit (Biolegend ...
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bioRxiv - Genomics 2022Quote: ... 106 cells were resuspended in PBS supplemented with 2% fetal bovine serum and incubated with human Fc blocking solution (BD Biosciences) for 10 minutes at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Human CD34+ stem cell purity was monitored by anti-human CD34 FITC (clone:581) and 7-AAD viability dye using LSR Fortessa X-20 flow cytometer (BD Biosciences).
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bioRxiv - Immunology 2022Quote: ... human cells were stimulated with PMA and ionomycin (500 ng/mL, eBioscience) in the presence of GolgiPlus and GolgiStop (BD Biosciences) for 4 h at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... IL-6 and IL-1β cytokines were measured in the cell culture supernatant after 2 h of co-exposure using CBA human Inflammatory cytokine kit (BD Biosciences), while chemokines CXCL8/IL-8 ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were then spun down and resuspended in 100LμL of buffer containing fluorescently conjugated antibodies for one hour (5LμL of each antibody per sample): APC-Cy7 Mouse Anti- Human CD4 (1:20 dilution; BD Biosciences, #557871), APC-Cy7 Mouse IgG1 ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then washed 2x with 1% FBS in PBS and incubated in 1% FBS in PBS with APC-conjugated mouse anti-human CD68 (BD Biosciences) for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... bone marrow aspirates were performed every 6 weeks post-transplant and human chimerism was tracked by flow cytometry analysis (using a BD LSR Fortessa analyzer or BD FACSymphony) and analyzed with the following ...
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bioRxiv - Genomics 2024Quote: ... day 7 cells were transferred to fresh culture medium with IL-2 and stimulated again with peptides and 1ug/mL of anti-human CD28 antibody (BD Biosciences) for another 7 days ...
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bioRxiv - Microbiology 2023Quote: ... for 16 hours followed by stimulation with a peptide pool of a broad range of HLA-subtypes and infectious agents (CEFX Ultra SuperStim Pool; JPT) (1 µg/ml) and 1 μg/ml mouse-anti-human CD28 antibody (#556620; BD Biosciences), or IL-2 (20 IU/ml ...
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bioRxiv - Biophysics 2024Quote: ... cells were washed twice in PBS + 2% FBS and stained with mouse anti-human CD45 Pe-Cy5 (BD Bioscience, clone HI30) and mouse anti-human CD107a Alexa Fluor 647 for 30 minutes at 4°C in the dark ...
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bioRxiv - Immunology 2022Quote: FACS-sorted Tregs were plated at 50,000 cells/well in round bottom 96 well plates pre-coated with anti-human CD3 (2 μg/ml, clone UCHT1, BD Biosciences) and soluble anti-human CD28 (1 μg/ml ...
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bioRxiv - Immunology 2023Quote: ... with the same panel of human antibodies as used for cell sorting and analyzed by flow cytometry on a FACS Fortessa (BD Biosciences). UltraComp eBeads (Invitrogen/Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... 10 µl of this bacterial suspension was added to the wells of a sterile 96-well flat-bottomed plate and incubated with 90 µl of freshly-donated human blood (collected in EDTA-treated tubes; BD Biosciences). Blood from each of four individual volunteers was assayed twice ...