Labshake search
Citations for Becton, Dickinson and Company :
1251 - 1300 of 1332 citations for 7 Cyano 5 fluoroindole since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... Then cells were washed twice with HBSS-5%FBS and once with PBS and analysed by flow cytometry (BD FACScalibur, BD Bioscience).
-
bioRxiv - Bioengineering 2023Quote: ... resuspended in 100 μL of PBS containing 5% FBS and blocked with 2.5 μg of Fc-blocking solution (BD Bioscience, Cat #564220) for 15 min at room temperature ...
-
bioRxiv - Cancer Biology 2022Quote: ... and 5% goat serum) for 1h at RT and then stained with EC specific CD31 antibody (BD biosciences, 550274, 1:25 dilution) or muscle base membrane laminin antibody (Sigma ...
-
bioRxiv - Biochemistry 2023Quote: ... Cells with the highest 2-5% GFP signal were selected two to three days post transfection using a flow cytometer (BD FACSAria II). Single cells were cultured in RPMI with 10% FBS ...
-
bioRxiv - Genomics 2023Quote: ... were then sorted into a 384-well plate containing 5 μL of autoclaved source water using FACS (BD FACSMelody™ Cell Sorter, BD Biosciences). 10 µL of RLT+ lysis buffer (Qiagen ...
-
bioRxiv - Neuroscience 2023Quote: ... we prepared a cranial window assembly by activating the surface of a circular glass coverslip (5 mm diameter, 0.13-0.15 mm thickness; Assistent Karl Hecht) with a laboratory corona treater (BD-20ACV Electro-Technic Products). We then fused the activated glass coverslip to a cylindrical silicone plug (1.5 mm diameter ...
-
bioRxiv - Neuroscience 2023Quote: ... Membranes were blocked with 5% skimmed milk in TBS-Tween and incubated with anti-αSyn clone 42 (BD Biosciences, 1:1,000 dilution). In a slot blot ...
-
bioRxiv - Immunology 2023Quote: ... and stained for the following surface markers on ice for 30 min: CD4 (APC-Cy7, 1:100, clone RM4-5, BD Biosciences, 565650), CD8b (Alexa Fluor 488 ...
-
bioRxiv - Cancer Biology 2023Quote: ... of 6 weeks old were inoculated subcutaneously (s.c.) with 5 × 105 cells mixed 1:1 with BD Matrigel Matrix High Concentration (BD Biosciences, Erembodegem, Belgium) previously diluted 1:1 in culture medium ...
-
bioRxiv - Immunology 2024Quote: ... and Parabacteroides distasonis were grown anaerobically at 37°C in brain heart infusion (BHI) media (Oxoid, UK) supplemented with hemin (5 mg/L) and vitamin K (0.5 mg/L) (BD, Franklin Lakes, NJ). Lactobacillus johnsonii and Ligilactobacillus murinus were grown anaerobically at 37°C in de Man Rogosa Sharpe (MRS ...
-
bioRxiv - Plant Biology 2020Quote: ... and 1 ml of cell suspension was incubated with 2 μl of Nile red solution (0.25 mg ml−1) for 5 minutes before the analysis with a bench flow cytometer (BD Accuri C6, BD Biosciences). To determine Nile red fluorescence ...
-
bioRxiv - Molecular Biology 2020Quote: ... then were seeded into a 96-well plate at a density of 5×103cells/well coated with 10mg/ml Matrigel matrix without growth factor (Cat No: 3432-010-01, BD Biosciences, CA, USA) for 6 hour incubation at 37□ ...
-
bioRxiv - Molecular Biology 2021Quote: ... slides were incubated for 60 minutes in 1% BSA and 5% goat serum in PBS and then incubated overnight with anti-p21 antibody (BD Pharmingen, mouse, #556431) and anti-p16 antibody (abbiotec ...
-
bioRxiv - Cancer Biology 2020Quote: ... 2000 cells were plated in 8-well chamber slides onto a base layer of growth factor-reduced Matrigel (5 mg/ml, BD Biosciences, Lot A6532) and collagen type I (1.5-2.1 mg/ml ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: The seeded cells (5 x 104 cells/mL) were pre-stained with 1 μL Orange NO probe (BD Biosciences, San Jose, CA, USA) per 500 μL cell suspension for 30 minutes ...
-
bioRxiv - Microbiology 2020Quote: ... Assay plates were incubated overnight at 37ºC in a 5% CO2 incubator and developed using BD ELISPOT AEC substrate set (BD Biosciences, San Diego, CA). Spots were counted and analyzed using an ELISPOT reader and Immunospot software (Cellular Technology ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were then blocked in PBS + 5% BSA for 1 hour before addition of primary antibodies (anti-LC3A/B, CST #4108, 1:100; anti-LAMP1, BD #555798, 1:100) diluted in blocking buffer overnight at 4°C ...
-
bioRxiv - Microbiology 2021Quote: ... Colonies were grown from frozen stocks by streaking on TSA-II agar plates supplemented with 5% sheep blood (BD BBL, New Jersey, USA). Unless otherwise stated ...
-
bioRxiv - Microbiology 2021Quote: ... or LB media (10 g/liter Bacto tryptone, 5 g/liter Bacto yeast extract, 10 g/liter sodium chloride; BD, San Jose, CA) at 30°C ...
-
bioRxiv - Immunology 2020Quote: ... cells were stained with PerCP/Cy5.5 or BV421-conjugated anti–mouse CD4 rat monoclonal antibody (RM4-5; BD Biosciences, Franklin Lakes, NJ, USA). The cells were then fixed and permeabilized with Cytofix/Cytoperm reagent (BD Biosciences ...
-
bioRxiv - Plant Biology 2019Quote: Surface-sterilized seeds were germinated and grown for 5 days containing 1% w/v sucrose and 1% w/v agar (Difco Bacto agar, BD laboratories; http://www.bd.com) in a growth chamber (NK system ...
-
bioRxiv - Microbiology 2019Quote: Colonies were grown from frozen stocks by streaking on TSA-II agar plates supplemented with 5% sheep blood (BD BBL, New Jersey, USA). Colonies were then picked and inoculated in fresh Columbia broth (Remel Microbiology Products ...
-
bioRxiv - Microbiology 2021Quote: ... Off-line samples were taken from the reactors each 5-10 h and the analysis of the GFP expression levels was performed with a BD Accuri C6 (BD Biosciences, CA, USA) based on FL1-A channel (excitation 488 nm ...
-
bioRxiv - Cancer Biology 2021Quote: ... Secondary antibody incubated for one hour at RT in 5% milk TBS-T at 1:1,000 (HRP-Anti-Mouse, BD Pharmigen, Goat Polycloncal, 554002). Between steps 3 × 5 minute washes were performed in TBST ...
-
bioRxiv - Neuroscience 2021Quote: ... the BECs were seeded in 96-well plates coated with collagen type I (5 µg/mL, #354231, BD Bioscience, San Jose, CA, USA) at a density of 104 cells per well ...
-
bioRxiv - Immunology 2021Quote: ... were resuspended in defRPMI-1640 with 3% newborn calf serum and Benzonase™ (FACS buffer) in 5 mL FACS tubes and pre-incubated with GolgiStop™ (BD Biosciences) for ∼60 min at 4°C ...
-
bioRxiv - Immunology 2021Quote: ... both ends of femurs and tibiae were cut with surgical scissors and bone marrow was extracted by flushing 3-5 ml sterile and ice-cold buffer through the bones using a 26G needle (BD Microlance 3 300300). The isolated bone marrow was then filtered through a 40 µM nylon cell strainer (FALCON 352340 ...
-
bioRxiv - Immunology 2022Quote: ... PBMCs were stimulated with the RBD peptide pool (5 μg.ml−1 final concentration) and brefeldin A (1 μg.ml−1 ; BD Pharmingen, San Diego, CA, USA) at 37 °C overnight ...
-
bioRxiv - Immunology 2022Quote: Surface staining: Single cell suspensions were incubated with 5% normal rat serum and rat anti-mouse CD16/CD32 antibody (BD Biosciences close 2.4G2) then stained with fluorophore-conjugated antibodies ...
-
bioRxiv - Immunology 2022Quote: ... 100 µL of detection antibody (IFNγ, BD Biosciences, #51-1818KA; IL-17A, Invitrogen, #88-7371-88; and IL-5, BD Biosciences, #51-1806KZ) was added at a 1:250 dilution in ELISpot diluent (eBioscience ...
-
bioRxiv - Microbiology 2022Quote: ... 98 mL of fresh MRS medium is then inoculated with 2 mL of the overnight culture and incubated at 37°C without shaking to an OD of 0.5-0.6 (ca. 4-5 hours) in a GasPak anaerobic jar (BD GasPak™ EZ container systems). Cells are harvested a first time by centrifugation at 4,000 g for 5 min ...
-
bioRxiv - Immunology 2023Quote: ... PerCP-Cy5.5-conjugated anti-IFN-γ (Clone 4S. B3, 1:10), and PE-cy7-conjugated anti-TNF-α (Clone Mab11, 1:50) (BD Biosciences) for intracellular staining ...
-
bioRxiv - Physiology 2023Quote: ... p = 0.2 (sum of shared targets between 1/0 and 5/0 R-βHB ex vivo groups and BH-BD targets, divided by n), and q = 0.8 ...
-
bioRxiv - Immunology 2023Quote: ... was added to the cell suspension and Fluorescein isothiocyanate (FITC)-positive to FITC-negative cell percentage was measured after 5 min of incubation using the BD FACSCanto™ Flow Cytometer (BD Biosciences, USA). Results were analyzed using the Flowing Software (version 2.5.1 ...
-
bioRxiv - Immunology 2023Quote: ... cells were washed 2x in FACS buffer (5% FBS, 2 mM EDTA in PBS) and resuspended with anti-CD16/CD32 Mouse BD FC Block (BD Biosciences, Cat. 553142) in FACS buffer at a concentration of 10 ug/ml for 15 min ...
-
bioRxiv - Immunology 2023Quote: ... and the cells were stained with antibodies specific for the following proteins: CD4 (PE Rat Anti-Mouse CD4; Clone RM4-5; BD Biosciences; Cat # 553049), H-2Dd (Anti-mouse H-2Dd antibody ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cell suspensions were blocked for 5 min at 4 °C with rat anti-mouse CD16/CD32 (#553142, Mouse BD Fc Block, BD Biosciences) in FACS buffer ...
-
bioRxiv - Cancer Biology 2023Quote: ... Membranes were blocked with 5% skim-milk diluted in 1X TBST and incubated with primary antibodies overnight [RB (BD Pharmingen #554136, 1:500), p53 (Pierce #MA5-12453 ...
-
bioRxiv - Microbiology 2024Quote: ... the cells were washed three times with PBS for 5 min each and then analyzed by flow cytometry (LSRFortessa, BD Biosciences, MD, USA). The mean fluorescence intensity (MFI ...
-
bioRxiv - Biophysics 2024Quote: ... After 5 or 10min of incubation at room temperature (including the 1min centrifugation) cells were fixed with BD fixation buffer (BD Biosciences 51-2090KZ) for 30min at room temperature ...
-
bioRxiv - Genomics 2024Quote: ... 2.5 x 105 cells were stained for 75 minutes at room temperature with PE Mouse anti-PDX1 Clone-658A5 (BD Biosciences; 1:10) and Alexa Fluor® 647 Mouse anti-NKX6.1 Clone R11-560 (BD Bioscience ...
-
bioRxiv - Microbiology 2024Quote: The urine sample obtained from the patient was quantitatively inoculated onto a Columbia agar plate with 5% sheep blood (BD Diagnostics, Heidelberg, Germany) and a MacConkey II-Agar plate (BD Diagnostics ...
-
bioRxiv - Neuroscience 2024Quote: ... The resuspended cells were incubated for 5 min on ice and sorted with an influx cell sorter (BD Biosciences, San Jose, CA, USA) with an 85 μm nozzle at 20 psi and 1X DPBS (Sigma-Aldrich ...
-
bioRxiv - Cancer Biology 2022Quote: ... The co-cultures were incubated for 6 hours at 37°C and 5% CO2 prior to cell staining using the Cytofix/Cytoperm Fixation Kit (BD Biosciences, Cat# 554715/555028).
-
bioRxiv - Neuroscience 2019Quote: ... Intracellular cytokine staining was performed according to the manufacturer’s protocol (Cytofix/Cytoperm buffer set from BD with PE-Cy7-conjugated anti-CD4 (RM4-5), eFluor 450-conjugated anti-TCR-β (H57-597) ...
-
bioRxiv - Immunology 2019Quote: ... Single-cell suspensions at 5 × 107 cells per ml were stained with a cocktail of fluorochrome conjugated human antibodies (BD Biosciences, San Jose, CA), anti-human IgG APC ...
-
bioRxiv - Immunology 2021Quote: ... Single leucocyte suspensions were stimulated with either phorbol 12-myristate 13-acetate (PMA)-ionomycin or 5 µg/ml of MCMV peptide pool (Table S3) for 6 hours with brefeldin A (BD Biosciences, San Jose, CA). After stimulation ...
-
bioRxiv - Neuroscience 2020Quote: ... infected mouse brain for flow cytometry were obtained as described.(Posel et al., 2016) Isolated cells were incubated overnight (5 hours for microglia analysis) in GolgiPlug and GolgiStop (BD Bioscience #555029 and #554724) in RPMI media containing HEPES and 10% fetal bovine serum (FBS ...
-
bioRxiv - Bioengineering 2020Quote: ... Cells were resuspended in PBS + 5% FBS and analyzed via Fluorescence-Assisted Cytometry (FACs) using a BD LSR Fortessa (BD Biosciences, San Jose, CA). Fluorescence minus one (FMO ...
-
bioRxiv - Microbiology 2020Quote: ... or 0 to 10−2 fluorescent intensity (AF647-negative) were captured and segregated in 2 separate 5 ml round-bottom tubes (BD Biosciences, San Jose, CA) containing 1% BSA/PBS ...