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Citations for Becton, Dickinson and Company :
1201 - 1250 of 1407 citations for Recombinant Human LAG3 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... bone marrow aspirates were performed every 6 weeks post-transplant and human chimerism was tracked by flow cytometry analysis (using a BD LSR Fortessa analyzer or BD FACSymphony ...
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bioRxiv - Immunology 2024Quote: ... LN or gut MNCs (after a period of resting at 37oC in X-vivo 15 continuing 1% human AB serum) using a BD FACSAria cell sorter (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were stained using a mouse anti-human antibody cocktail to cell surface markers: CD3-BV711 (BD Biosciences, clone UCHT1), CD14-BV711 (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... or H226 (5×106) human cells were subcutaneously injected into the right flank of each mouse with an equal volume of Matrigel (BD) to PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... 5×104 alive GAFs or glioma cells were incubated with PE Mouse anti-Human CD105 (2µg/mL, BD Biosciences #560839) and APC Mouse anti-Human CD90 (2µg/mL ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were blocked with 0.1 mg/ml mouse IgG prior to staining with BV-421 labeled anti-human CCR6 antibody (Clone 11A9, BD Biosciences) for 1 h at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... The following anti-human antibodies were used for intracellular cytokine staining: anti-IL-8 PE (clone G265-8, BD Bioscience), anti-IFN-γ APCvio770 (clone REA600 ...
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bioRxiv - Immunology 2023Quote: ... PBMCs were stained using a mouse anti-human antibody cocktail to cell surface markers: CD3-BV711 (BD Biosciences, clone UCHT1), CD14-BV711 (BD Biosciences ...
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bioRxiv - Neuroscience 2023Quote: Erythroblasts and iPSCs were washed and resuspended in FC buffer (HBSS w/o calcium and magnesium + 0.5% BSA) and incubated with PE Mouse Anti-Human CD44 antibody (1:25, BD, 550989), FITC Mouse Anti-Human CD71 antibody (1:50 ...
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bioRxiv - Immunology 2022Quote: The following anti-human antibodies were used for surface staining: Anti-CD56 Brilliant Violet (BV) 650 (clone NCAM 16.2, BD Bioscience), anti-CD39 BV421 (clone A1 ...
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bioRxiv - Bioengineering 2023Quote: ... The cells were then permeabilized using 0.1% Triton X-100 and stained with anti-CD68 antibody (PE- Mouse Anti-Human CD68 set, BD Biosciences) (1:50 dilution ...
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bioRxiv - Molecular Biology 2023Quote: ... were coated overnight at 4 °C with 300 ng/mL mouse anti-human RIPK1 capture antibody (1:100 dilution in PBS of BD Bioscience BD610459 lot 0314064 ...
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bioRxiv - Immunology 2023Quote: ... were cultured in R10 in the presence of human GM-CSF (10 ng/ml, Miltenyi) and IL-4 (1000 U/ml, BD) for 5 days as previously described 24 ...
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bioRxiv - Cell Biology 2023Quote: Cytokine released by the SARS-CoV-2-infected or ApoBD-treated MDMs were measured using human cytokine bead array as per manufacturer’s instruction (BD Biosciences). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: Purified mouse anti-human CD63 antibody (Catalog No. 556019) and isotype control antibody (Catalog No. 555746) were bought from BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... Cells were stained with pre-optimized concentrations of the following antibodies: PE anti-human IgD (clone IA6-2, BD Biosciences), PE-TXRD anti-human CD10 (clone HI10A ...
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bioRxiv - Immunology 2024Quote: Blood samples from healthy volunteers to obtain ABO-matched normal human serum were collected in vacutainer tubes for serum analysis (ref. no. 367896, BD).
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bioRxiv - Immunology 2024Quote: ... in the presence of 100 U/ml of human IL-2 and soluble anti-CD28 antibody (BD Biosciences, Clone: 37.51). T lymphoblasts were cultured at 37 °C in 5% CO2 for 3 days in exponential growth phase ...
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bioRxiv - Systems Biology 2020Quote: ... White cells were resuspended in PBS supplemented with 1% human serum and 2 mM EDTA and analyzed on an LSR Fortessa instrument (BD Biosciences).
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bioRxiv - Immunology 2021Quote: Supernatants were collected 4 or 24 h post-challenge and the levels of TNF and mature IL-1β were quantified by ELISA using OptEIA human TNF ELISA Set (BD Biosciences) and human IL-1β/IL-1F2 DuoSet ELISA (R&D systems) ...
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bioRxiv - Immunology 2021Quote: CD47 expression or cross-dressing on cells was determined by direct staining with BV786-conjugated anti human specific CD47 mAb B6H12 (abbreviated anti-hCD47; BD Bioscience) or PE-conjugated anti-human CD47 mAb CC2C6 (with cross-reactivity to pig CD47 and thus ...
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bioRxiv - Microbiology 2020Quote: Macrophages were infected in a 48-well plate as described above and harvested supernatants were assayed for cytokine levels using ELISA kits for human IL-1β (BD Biosciences) and IL-18 (R&D Systems).
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bioRxiv - Molecular Biology 2021Quote: ... Cells were re-suspended in 100 μl of FCSB and 1 μl of mouse anti-human CD10-PE (561002, BD Biosciences) and mouse anti-human CD92-AF647 (565316 ...
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bioRxiv - Neuroscience 2022Quote: Dissociated and resuspended iTF-Microglia were blocked for 15 min with 1:20 Human FC Block (BD Biosciences; Cat. No. 564220) and then stained with 1:66 PE/Cy7 anti-human CD184 (CXCR4 ...
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bioRxiv - Immunology 2022Quote: ... NK cells were added to the washed ELISA plate and incubated together with anti-human CD107a (BD, 1:40, clone: H4A3), brefeldin A (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... primary hMDMs were transfected in a 48-well plate as described above and harvested supernatants were assayed for cytokine levels using an ELISA kit for human IL-1β (BD Biosciences) according to the manufacturer’s instructions.
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bioRxiv - Bioengineering 2019Quote: ... with anti-positive (CD90, CD105, CD44) and negative marker (CD34, CD11b, CD19, CD45, MHC class II) antibodies (Human MSC Analysis Kit, 562245, BD Biosciences), as described in detail in [42] ...
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bioRxiv - Developmental Biology 2019Quote: ... Cell suspensions from APEL vascular differentiations were incubated with mouse anti-human CD31-APC (eBioscience, San Diego, CA) and CD146-PE (BD Biosciences) antibodies for 30 min on ice ...
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bioRxiv - Molecular Biology 2019Quote: ... The HLA-DQ cell surface expression was performed by cytofluorimetric analysis using the FACSAria III and DIVA software with FITC mouse anti-human HLA-DQ antibody (BD Biosciences). The quantitation of specific transcripts was performed by qRT-PCR as described in [9] ...
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bioRxiv - Cancer Biology 2019Quote: ... cKIT and PDGFRA expression was assessed using mouse anti-human CD117 (PE-Cyanine7, clone 104D2, #25-1178-42, eBioscience) and CD140a (PE, clone αR1, #556002, BD Pharmingen) respectively ...
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bioRxiv - Cancer Biology 2019Quote: ... Membranes were blocked for 1 h in 5% milk/TBS Tween-20 solution and incubated for 1 h with anti-TOP2β (1:5,000, mouse anti-human, Clone 40/Topo IIβ, RUO – 611492, BD Biosciences-US) or anti-TOP2α (1:10,000 ...
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bioRxiv - Microbiology 2019Quote: ... Cells were then washed three times in PBS followed by incubation in 1% Human Fc Block (BD Biosciences, San Jose, CA) in PBS ...
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bioRxiv - Cell Biology 2019Quote: ... cells were stained with a 1:50 dilution of phycoerythrin (PE)-conjugated mouse monoclonal antibody against human TRAIL (550516, BD Biosciences). For immunophenotyping of MSCs the cells were stained with 1:50 dilution of fluorochrome-conjugated mouse monoclonal antibodies against CD73 (PE-Vio770 ...
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bioRxiv - Bioengineering 2020Quote: ... Cytokine concentrations in the culture supernatant were measured using BD Cytometric Bead Array Human Th1/Th2 Cytokine Kit II (BD Biosciences).
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bioRxiv - Immunology 2021Quote: ... Cytokines in the serum sample were measured using human Th1/Th2/Th17 cytometric bead array kit (BD Bioscience, San Jose, CA) according to the manufacture’s instruction ...
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bioRxiv - Immunology 2021Quote: ... labelled with anti-human myeloid and stromal markers (Table 1) and analysed by flow cytometry on a LSR Fortessa (BD Biosciences).
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bioRxiv - Immunology 2020Quote: ... the PBMCs were stimulated with pooled or individual peptides at a final concentration of 10 μg/mL per individual peptide for 1 h in the presence of 2 μg/mL monoclonal antibodies against human CD28 (BD Pharmingen) and CD49d (BD Pharmingen ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were then washed twice 5 min in Tris buffer saline containing 0.01% Triton X-100 (TBST) and incubated with the primary antibody over night at 4°C (human and mouse a-Syn: 610786 BD Biosciences). After incubation with the first antibody ...
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bioRxiv - Neuroscience 2020Quote: ... Human CD34C-derived ECs were seeded at a density of 8×104/insert onto the Matrigel-coated (BD Biosciences, United States) Transwell inserts (3401-Costar ...
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bioRxiv - Cancer Biology 2019Quote: ... and human prostate cancer propagating cells were resuspended in 120 µl of a 1:1 mixture of Matrigel (BD Biosciences, #354234) and PrEGM (Lonza ...
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bioRxiv - Microbiology 2020Quote: ... adjusted to 106 CFU ml-1 in HBSS and 104 CFU (10 μl) used to inoculate 90 μl of freshly donated human blood (collected in EDTA-treated tubes; BD Biosciences) or freshly diluted H2O2 (10 mM in PBS ...
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bioRxiv - Microbiology 2020Quote: ... IL-10 and IL-12p70 were quantified in those culture supernatants by ELISA with BD OptEIA™ human IL-10 and IL-12p70 sets following the manufacturer's instructions (BD Bioscience).
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bioRxiv - Genomics 2021Quote: ... hPBMCs were resuspended in 1x PBS + 2% FBS and stained with a FITC-conjugated Mouse Anti-Human CD45 Antibody (Clone HI30, BD Biosciences) for 25 min on ice ...
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bioRxiv - Immunology 2020Quote: ... 8.0 × 105 cells per condition were fixed in 1% PFA for 10 min at room temperature and subsequently stained with primary antibodies: mouse anti-human PEcy7-CD16 (BD #557744), mouse anti-human PerCPcy5.5-CD66b (Biolegend # 305107) ...
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bioRxiv - Microbiology 2021Quote: ... Intracellular staining of the cytokines was performed after fixation and permeabilization using APC conjugated anti-human IFNγ (BD Bioscience, Cat# 551385), anti-human IL2 (BD Bioscience ...
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bioRxiv - Cancer Biology 2021Quote: ... Flow cytometry for negative checkpoint regulator was performed as previously described(14) using antibodies: BV510 mouse anti-human CD279 (PD1) (1:100; 563076, BD Horizon), PerCP-eFluor 710 mouse anti-human CD223 (LAG-3 ...
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bioRxiv - Molecular Biology 2022Quote: Human embryonic stem cell (hESC) line H9 and human induced pluripotent stem cell (hiPSC) line S3 were cultured on Matrigel (BD Biosciences)-coated plates in mTesR1 medium (19 ...
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bioRxiv - Immunology 2022Quote: ... For measurements of CXCL13 the cells were cultured for 5 hours at 37°C in RPMI supplemented with 10% human AB serum and GolgiStop (1/1500; BD Biosciences). For cytokine measurements the cells were plated in the presence of anti-CD3/CD28 (Dynabeads® Human T-activator CD3/CD28 ...
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bioRxiv - Immunology 2022Quote: ... then cell culture supernatant were harvested and analysed using a BD Cytometric Bead Array (CBA) Human Soluble Protein Master Buffer Kit (BD Biosciences) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... cells were resuspended in 90 μL staining buffer and incubated with 10 μL of anti-human CD21-PE (Cat #555422, BD Bioscience), anti-human CD55-PE (MHCD5504 ...