Labshake search
Citations for Becton, Dickinson and Company :
1201 - 1250 of 2077 citations for Recombinant Human IFNA5 Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... One million cells per sample were pre-incubated with purified anti-mouse CD16/CD32 antibody (Fc Block™, 553141, BD Biosciences). Live/dead cells were discriminated by staining cells with the LIVE/DEAD® Fixable Near-IR Dead Cell Stain kit (L10119 ...
-
bioRxiv - Immunology 2022Quote: BMDMs were harvested as described before and Fc receptors were blocked with an anti-mouse CD16/CD32 antibody (1:250, BD Biosciences) in FACS buffer (5 % heat-inactivated FCS ...
-
bioRxiv - Immunology 2019Quote: Lymphocytes or purified splenic B cells were washed in PBS with 1% BSA and incubated with 1mg/ml purified rat anti-mouse CD16/CD32 (Fc block, BD Pharmingen) at 4°C for 15 min ...
-
bioRxiv - Neuroscience 2021Quote: ... Fc receptors were blocked as described above and cells were stained with CD3e FITC (1:200, clone 145-2C11, BD Pharmingen) or CD3e PE-Cy7 (1:200 ...
-
bioRxiv - Bioengineering 2021Quote: ... Cells were incubated for 15 minutes at room temperature in the dark in 50uL PBS with Fc Block (1/100 dilution, BD, 564220) and Zombie UV dye (1 in 500 dilution ...
-
bioRxiv - Immunology 2021Quote: ... B cells were isolated by negative selection and resuspended in FACS buffer (PBS with 2% Fetal calf serum) followed by staining with anti-mouse CD16/CD32 (mouse Fc block; BD Biosciences) and a decoy tetramer (BV510 ...
-
bioRxiv - Immunology 2021Quote: ... Cells were aliquoted to a count of 1 million cells each and incubated on ice with Fc Block (BD, Table S1) at a 1:20 dilution ...
-
bioRxiv - Neuroscience 2021Quote: ... and their Fc receptors were blocked for 10 min at 4 °C with anti-CD16/CD32 antibodies (10 μg/mL: BD Biosciences). After blocking ...
-
bioRxiv - Immunology 2020Quote: ... For flow cytometry analysis first Fc receptors were blocked using α-CD16/CD32 antibody (clone 2.4G2, BD Biosciences, Bedford, MA, USA). The cells were subsequently washed and stained for following surface markers with directly conjugated antibodies against CD19/B220 (PerCP Cy5.5 or eFluor 450) ...
-
bioRxiv - Microbiology 2021Quote: Spleen and liver cells were incubated (20 min, 4°C) with anti-Fc-gamma blocking antibody (clone 2.4G2) (BD Biosciences, Belgium) and were subsequently stained with fluorescent conjugated antibodies (30 min ...
-
bioRxiv - Immunology 2019Quote: ... 10 ml PBS with 10 % FCS (buffer A) was added and the suspension was passed through a 100 µm cell strainer (BD Biosciences). The resulting single cell suspension was pelleted and resuspended in 1 ml ACK buffer (0.15 M NH4Cl ...
-
bioRxiv - Immunology 2020Quote: ... Cells were stained with fluorescent-conjugated anti-mouse antibodies after blocking CD16/CD32 with Fc Block (BD Biosciences, San Jose, CA). For intracellular staining ...
-
bioRxiv - Immunology 2019Quote: ... and CD3+CD4+CD25− cell population (Tconv) were directly sorted into tubes with 500 μL FCS on a FACSAria™ III (BD). For RNA sequencing ...
-
bioRxiv - Immunology 2020Quote: ... cells were resuspended in 4% heat-inactivated FCS containing FACS buffer and data were acquired by a Symphony Flow Cytometer (BD Biosciences). Flow cytometry and t-SNE analyses were performed with FlowJo software (FlowJo10.5.3) ...
-
bioRxiv - Immunology 2020Quote: ... then washed with 2% FCS - PBS and stained with Mouse anti-IRF-7-PE (pS477/pS479, clone K47-671; BD Biosciences) or Mouse anti-NF-κB p65-Alexa Fluor 647 (pS529 ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were then blocked for 5 minutes at room temperature using anti-mouse CD16/32 Fc block (1/50) (BD Biosciences). Cells were then incubated with the staining (Ly6g-APCCy7 (1A8) ...
-
bioRxiv - Cell Biology 2020Quote: ... anti-Synapsin (1:500) and anti-CD133 (1:500) were used after Fc blocking with CD16/32 antibody (553141; BD Pharmingen). Respective Secondary antibodies ...
-
bioRxiv - Immunology 2020Quote: Cells isolated from the organs indicated in figure 4 and figure S9 were incubated with an Fc blocking agent (BD Pharminogen) for 10min at 4°C ...
-
bioRxiv - Immunology 2021Quote: ... The bacterial pellet was resuspended in 1X PBS + 0.25% BSA + 5% NGS and stained with Fc Block (BD Biosciences, clone 2.4G2), followed by SYTO BC (ThermoFisher ...
-
bioRxiv - Immunology 2020Quote: ... and incubated with FC block and subsequently stained with a combination of the following anti-mouse antibodies (all from BioLegend, eBioscience, or BD Biosciences): anti-CD8α (53-6.7) ...
-
bioRxiv - Immunology 2021Quote: ... Cells were stained with fluorescent labelled anti-mouse antibodies after blocking CD16/CD32 with Fc Block (BD Biosciences, San Jose, CA). Flow cytometry analysis was performed using following antibodies ...
-
bioRxiv - Immunology 2020Quote: ... The cells were washed twice with PBS containing 2% FBS and were blocked with CD16/32 Fc block (Cat. 553142, BD Bioscience) before the analysis ...
-
bioRxiv - Immunology 2020Quote: ... Up to 5×106 cells were washed in cold FACS wash (PBS supplemented with 2% FCS and 2mM EDTA) prior to being stained with a surface receptor antibody cocktail containing FcBlock (BD, 2.4G2) and LIVE/DEAD fixable blue dead cell stain (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2022Quote: For 3T3 NIH fibroblasts cells infected with C/EBPα-ER-IRES-hCD4 (WT and T35A) were incubated with 0.1µg per 1 million cells of both Fc block and hCD4-Biotin antibody (BD Pharmingen, Cat#555347) for 10 and 20 minutes respectively ...
-
bioRxiv - Immunology 2022Quote: ... and were resuspended in Fc stain buffer prior to performing flow cytometry analysis on a LSR Fortessa flow cytometer (BD Biosciences) and analyzed using FlowJo software (version 10.7.1) ...
-
bioRxiv - Immunology 2022Quote: ... For quantification of the different developmental steps in T cell maturation the cells were blocked with murine Fc-block (BD Biosciences) and stained with either CD45-APC/Cy7 (BD Biosciences ...
-
bioRxiv - Biochemistry 2023Quote: ... for all experiments except for experiment with soluble Siglec-7-Fc constructs in which a 5-laser Fortessa X-20 (BD Biosciences) was used instead.
-
bioRxiv - Neuroscience 2023Quote: ... all samples were filtered through a 70-μm nylon mesh and blocked with anti-Fc CD16/32 (1:40; BD Biosciences). For FOXP3 staining ...
-
bioRxiv - Microbiology 2023Quote: ... 1 x 106 parasites were collected and incubated with VSG2WT antisera (1:4000) or VSG11WT (1:1000) together with Fc block (1:200, BD Pharmingen) in cold HMI-9 without FBS for 10 min at 4°C ...
-
bioRxiv - Immunology 2023Quote: ... CD45—CD38—CD235a—CD71— non-hematopoietic cells were sorted in DMEM supplemented with 10% FCS using a FACSAria III (BD Biosciences) and BD FACSDiva version 5.0 (BD Biosciences).
-
bioRxiv - Neuroscience 2023Quote: ... cells were incubated for 15 min at 4 °C with a purified mouse anti-rat CD32 FcγII (clone D34-485, Rat BD Fc Block) to block unspecific binding ...
-
bioRxiv - Cell Biology 2023Quote: Unfractionated BM cells were counted on the XP-300-Hematology Analyzer (Sysmex Corporation) and then Fc-blocked either with purified CD16/32 (BD Biosciences), or stained with CD16/32 antibody conjugated to a fluorophore ...
-
bioRxiv - Immunology 2023Quote: ... Cells were stained on ice for 30min in FACS buffer with 1:100 dilutions of biotin-conjugated-HA and purified rat anti-mouse CD16/CD32 (Mouse BD Fc Block™, Clone 2.4G2, BD Biosciences), then stained on ice for 30min in FACS buffer with 1:1000 dilution of allophycocyanin (APC)-conjugated streptavidin ...
-
bioRxiv - Cancer Biology 2023Quote: Single cell suspensions were pre-incubated for 15 minutes in a staining solution (PBS+/+, 2% FBS) containing 1 μg/mL Fc block (BD Biosciences), followed by an incubation with the antibodies for 45 minutes (Table S1) ...
-
bioRxiv - Immunology 2023Quote: ... at 1:600 antibody to cell suspension volume ratio in 30×106 cell/ml HBH with Fc block for 15-30 min on ice and then sorted with a BD FACS Aria III (BD Biosciences) with assistance from the University of Washington Pathology Flow Cytometry Core Facility ...
-
bioRxiv - Biophysics 2022Quote: ... The FITC ERF values were assigned to both 2 μm and 6 μm beads using a specific lot of FITC-FC Bead (BD Biosciences) as a calibrator with known ERF value ...
-
bioRxiv - Cancer Biology 2023Quote: ... and 7AAD-CD45+CD34-CD117-CD33- non-myeloid (lymphoid) fraction were sorted in DMEM supplemented with 10% FCS using a FACSAria III (BD Biosciences) and BD FACSDiva version 5.0.
-
bioRxiv - Immunology 2022Quote: ... Single-cell suspensions were incubated with Fc block (anti-mouse CD16/CD32, 1 μg for 1x106 cells, BD Biosciences, Cat# 553142) at 4°C for 30 min ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cell suspensions were blocked for 5 min at 4 °C with rat anti-mouse CD16/CD32 (#553142, Mouse BD Fc Block, BD Biosciences) in FACS buffer ...
-
bioRxiv - Immunology 2023Quote: ... Cells were acquired in BD LSR-II flow cytometer using FACS diva software and FCS files were analyzed by the Flowjo v10.5.0 software (BD Biosciences, CA, USA). For absolute counting ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were washed two times with 150 µL PBS (centrifuged 5 min at 400 × g at 4 °C) and resuspended in 50 µL Fc-block (1:50, Purified Rat Anti-Mouse CD16/CD32, BD, #553142) diluted in FACS buffer (1x PBS/2% FBS/2mM ...
-
bioRxiv - Neuroscience 2023Quote: ... cell suspension was incubated with Fc block antibodies for 5 minutes at 4 °C and then labeled with anti-mouse Thy1.2 magnetic particles (BD IMag™) for 30 minutes at 4 °C ...
-
bioRxiv - Bioengineering 2020Quote: ... we preincubated 2 µmol recombinant B56 protein with 1 µmol mouse anti-B56 antibody (Clone 23/B56α BD biosciences, 610615) and 1 µmol goat anti-mouse IgG Alexa Fluor 647 (Thermo Fisher A-21235 ...
-
bioRxiv - Immunology 2020Quote: ... and then labeled cells were incubated in the presence of recombinant IL-15/15Rα (50ng/mL) in the presence of absence of anti-integrin α1 (10μg/mL, BD Biosciences) or Integrin α1β1 inhibitor Obtusatin (10nM ...
-
bioRxiv - Immunology 2021Quote: ... FNA samples were stained in P2 for 30 minutes on ice with biotinylated and Alexa Fluor 647 conjugated recombinant soluble Spike proteins as well as PD-1 BB515 (EH12.1, BD Horizon). Cells were then washed twice with P2 and stained with IgG BV480 (goat polyclonal ...
-
bioRxiv - Microbiology 2022Quote: ... were transformed with recombinant plasmids per manufacturer instructions and grown at 37°C on selective LB agar plates (BD, 244520) and in LB broth (Fisher ...
-
bioRxiv - Microbiology 2020Quote: Human lymphocyte characterization was performed on an LSR II (BD Biosciences). Lungs from BLT mice were minced using scissors and incubated in medium containing Liberase (0.02 mg/mL ...
-
bioRxiv - Molecular Biology 2019Quote: ... The membrane was subsequently incubated with mouse anti-human CD63 (BD Biosciences ...
-
bioRxiv - Cancer Biology 2019Quote: ... Anti-human CD33 (Clone WM 53, # 555450) was obtained from BD Biosciences ...
-
bioRxiv - Cell Biology 2019Quote: ... APC-conjugated mouse anti-human EPCAM (1:20; EBA-1; BD), PE/Cy7-conjugated anti-human/mouse CD49f (ITGA6 ...