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Citations for Becton, Dickinson and Company :
1201 - 1250 of 1385 citations for Recombinant Human ABHD15 His tagged since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... primary hMDMs were transfected in a 48-well plate as described above and harvested supernatants were assayed for cytokine levels using an ELISA kit for human IL-1β (BD Biosciences) according to the manufacturer’s instructions.
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bioRxiv - Bioengineering 2020Quote: ... Cytokine concentrations in the culture supernatant were measured using BD Cytometric Bead Array Human Th1/Th2 Cytokine Kit II (BD Biosciences).
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bioRxiv - Immunology 2021Quote: ... Cytokines in the serum sample were measured using human Th1/Th2/Th17 cytometric bead array kit (BD Bioscience, San Jose, CA) according to the manufacture’s instruction ...
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bioRxiv - Immunology 2021Quote: ... labelled with anti-human myeloid and stromal markers (Table 1) and analysed by flow cytometry on a LSR Fortessa (BD Biosciences).
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bioRxiv - Immunology 2020Quote: ... the PBMCs were stimulated with pooled or individual peptides at a final concentration of 10 μg/mL per individual peptide for 1 h in the presence of 2 μg/mL monoclonal antibodies against human CD28 (BD Pharmingen) and CD49d (BD Pharmingen ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were then washed twice 5 min in Tris buffer saline containing 0.01% Triton X-100 (TBST) and incubated with the primary antibody over night at 4°C (human and mouse a-Syn: 610786 BD Biosciences). After incubation with the first antibody ...
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bioRxiv - Neuroscience 2020Quote: ... Human CD34C-derived ECs were seeded at a density of 8×104/insert onto the Matrigel-coated (BD Biosciences, United States) Transwell inserts (3401-Costar ...
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bioRxiv - Microbiology 2020Quote: ... adjusted to 106 CFU ml-1 in HBSS and 104 CFU (10 μl) used to inoculate 90 μl of freshly donated human blood (collected in EDTA-treated tubes; BD Biosciences) or freshly diluted H2O2 (10 mM in PBS ...
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bioRxiv - Microbiology 2020Quote: ... IL-10 and IL-12p70 were quantified in those culture supernatants by ELISA with BD OptEIA™ human IL-10 and IL-12p70 sets following the manufacturer's instructions (BD Bioscience).
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bioRxiv - Genomics 2021Quote: ... hPBMCs were resuspended in 1x PBS + 2% FBS and stained with a FITC-conjugated Mouse Anti-Human CD45 Antibody (Clone HI30, BD Biosciences) for 25 min on ice ...
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bioRxiv - Immunology 2020Quote: ... 8.0 × 105 cells per condition were fixed in 1% PFA for 10 min at room temperature and subsequently stained with primary antibodies: mouse anti-human PEcy7-CD16 (BD #557744), mouse anti-human PerCPcy5.5-CD66b (Biolegend # 305107) ...
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bioRxiv - Microbiology 2021Quote: ... Intracellular staining of the cytokines was performed after fixation and permeabilization using APC conjugated anti-human IFNγ (BD Bioscience, Cat# 551385), anti-human IL2 (BD Bioscience ...
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bioRxiv - Cancer Biology 2021Quote: ... Flow cytometry for negative checkpoint regulator was performed as previously described(14) using antibodies: BV510 mouse anti-human CD279 (PD1) (1:100; 563076, BD Horizon), PerCP-eFluor 710 mouse anti-human CD223 (LAG-3 ...
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bioRxiv - Molecular Biology 2022Quote: Human embryonic stem cell (hESC) line H9 and human induced pluripotent stem cell (hiPSC) line S3 were cultured on Matrigel (BD Biosciences)-coated plates in mTesR1 medium (19 ...
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bioRxiv - Immunology 2022Quote: ... For measurements of CXCL13 the cells were cultured for 5 hours at 37°C in RPMI supplemented with 10% human AB serum and GolgiStop (1/1500; BD Biosciences). For cytokine measurements the cells were plated in the presence of anti-CD3/CD28 (Dynabeads® Human T-activator CD3/CD28 ...
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bioRxiv - Immunology 2022Quote: ... then cell culture supernatant were harvested and analysed using a BD Cytometric Bead Array (CBA) Human Soluble Protein Master Buffer Kit (BD Biosciences) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... cells were resuspended in 90 μL staining buffer and incubated with 10 μL of anti-human CD21-PE (Cat #555422, BD Bioscience), anti-human CD55-PE (MHCD5504 ...
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bioRxiv - Biochemistry 2022Quote: ... TCR expression on transfected Jurkat cells was assessed by staining with phycoerythrin (PE)-conjugated mouse anti-human CD3 (BD Pharmingen 555333); TCR expression was unaltered by Csk overexpression.
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bioRxiv - Immunology 2020Quote: ... TNF-α and IFN-γ in the serum of experimental monkeys were detected with the Non-Human Primate (NHP) Th1/Th2 Cytokine Kit (BD) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... sequenced reads were aligned to the hg38 human transcriptome and the expression of transcripts in each cell was quantified via the standard Rhapsody analysis pipeline (BD Biosciences) on Seven Bridges (https://www.sevenbridges.com) ...
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bioRxiv - Immunology 2020Quote: PBMC were labelled for FACS staining with mouse anti-human antibodies directed against lineage markers (Vioblue-CD3, CD14, CD19, CD56 and CD11c) (BD Biosciences), APC-Vio770 HLA-DR ...
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bioRxiv - Immunology 2020Quote: ... followed by staining for 3 days at 37 °C with 1:100 dilutions of BV421-labeled mouse anti-human CD35 clone E11 (BD Biosciences) and Alexa Fluor 488-labeled mouse anti-Ki67 clone B56 (BD Biosciences ...
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bioRxiv - Biochemistry 2020Quote: ... to stain for CD69 receptor using 5 µL (100 µg/mL) of PreCP-Cy ™ 5.5 mouse anti-human CD69 (BD Biosciences). Staining was done for 45 mins on ice then washed one time with 2 mL RPMI-1640 medium and reconstituted in 250 µL RPMI-1640 medium ...
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bioRxiv - Neuroscience 2022Quote: ... Syn1 [a mouse monoclonal antibody that recognizes residues 91-99 from the NAC region of human α-synuclein (51)] (BD Biosciences) at 1:4,000 and PER4 at 1:4,000 ...
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bioRxiv - Immunology 2022Quote: ... The membranes were then incubated with human mAbs (at a concentration of 1 µg/ml) and mouse anti-Hisx6 antibody (1 µg/ml, BD Biosciences) in PBS-T 5% dry milk for 2 h ...
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bioRxiv - Immunology 2022Quote: ... and then incubated with human mAbs (at a concentration of 1 µg/ml) and mouse anti-Hisx6 antibody (1 µg/ml, BD Biosciences) in PBS-T 5% dry milk in each channel for 2 h ...
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bioRxiv - Immunology 2022Quote: ... whereas 100 µl PBS+/+ containing 20 ng/ml recombinant IL-1β supplemented with 100 µg/ml PTX3 and/or 100 µg/ml mouse anti-human CD62P (clone AK-4, BD Bioscience) was added to the apical side (upper chamber) ...
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bioRxiv - Cancer Biology 2022Quote: ... For DAC studies on human MPNST xenograft bearing CB-17 SCID mice (Taconic): all cells were implanted into mice 1:1 with Matrigel (BD Biosciences). M3 cells (stably infected with MSCV-Luciferase-PGK-Hygro retrovirus ...
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bioRxiv - Genetics 2020Quote: Human embryonic stem (hES) H1 cells (WA01, WiCell) were maintained under feeder free conditions on Matrigel-coated plates (#354277, BD Biosiences) in mTeSR™1 ...
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bioRxiv - Immunology 2020Quote: ... Cells with SARS-CoV-2 peptides and positive control were co-stimulated with CD28/CD49d at a final concentration of 5 μg/ml (anti-human CD28/CD49d Purified Clone L293 L25 BD Biosciences). Basal IFN-γ production by PBMCs was included as background control in the absence of stimulation and co-stimulation ...
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bioRxiv - Immunology 2021Quote: Interleukin-8 secretion of treated THP-1 monocytes was quantified by the ELISA OptEIA™ Set for human interleukin-8 (BD) following the manufacturer’s protocol with optimized wash buffer composition (50x dilution) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... activation of T-cells was measured by flow cytometry using the anti-human CD4 (FITC)/CD69 (PE)/CD3 (PerCP) antibody staining solution (BD Biosciences) in combination with anti CD25 (APC ...
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Enhancer Remodeling Promotes Tumor-Initiating Activity in NRF2-Activated Non-Small Cell Lung CancersbioRxiv - Cancer Biology 2020Quote: ... and human tumor cells were collected as the mouse MCH class I-negative fraction using flow cytometry (BD FACSAria, Becton Dickinson). Then ...
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bioRxiv - Pathology 2021Quote: Cells were fixed in 2% PFA for 15 minutes at room temperature and incubated with mouse anti-human VE-Cadherin (BD Sciences) at 4°C overnight as previously described 36 ...
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bioRxiv - Immunology 2020Quote: ... samples were first pre-incubated with human BD Fc Block to block non-specific binding (BD Pharmingen, Franklin Lakes, NJ, USA) for 20 minutes at room temperature ...
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bioRxiv - Immunology 2021Quote: Cells were co-cultured in R10 medium alone or in presence of K562 cells for 6 hours in the presence of anti-human CD107a (H4A3, FITC, BD Biosciences). Golgi plug and Golgi stop (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: Supernatants were collected after 4 or 24 h and the levels of TNF and IL-1β were quantified by ELISA using OptEIA human TNF ELISA Set (BD Biosciences) and human IL-1β/IL-1F2 DuoSet ELISA (R&D systems ...
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bioRxiv - Immunology 2021Quote: ... or with no target cells (or an E:T of 1:0) and cultured overnight before being staining with allophycocyanin (APC)-conjugated anti human-CD69 antibody (BD Biosciences #555533). Flow cytometry was performed using a BD- Fortessa (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... T cells from different human donors (P1, P2, P3) were isolated using the RosetteSep™ negative isolation method and activated (using BD Human T cell activator beads coated with anti-CD3 and anti-CD28 ...
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bioRxiv - Immunology 2020Quote: Cells were washed in PBS and resuspended in PBS containing 2% fetal calf serum and 2 mM EDTA and stained with the extracellular mix using anti-human CD14-BV421 antibody (clone M5E2, BD Bioscience) and anti-human CD36-BUV605 antibody (clone CD38 ...
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bioRxiv - Immunology 2021Quote: ... To this end 1×104 targets per well and 2×104 effector cells per well were each preincubated for 2 h in 10 μg/ml anti-human CD178 antibodies (NOK-1 and NOK-2, BD Biosciences) or 5 μg/ml anti-human TRAIL antibody (RIK-2 ...
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bioRxiv - Genomics 2020Quote: Immune cells for bulk mRNA sequencing were incubated with Fc Block for 20 minutes and then stained with one of six panels of directly conjugated antibodies for 30 minutes: anti-human CD16 (BD 558122), CD123 (BD 560826) ...
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bioRxiv - Immunology 2021Quote: ... anti-mouse IFN-γ ELISA set (Cat no. – 555138) and anti-human IL-10 ELISA set (Cat no. – 555157) were purchased from BD Biosciences ...
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bioRxiv - Genomics 2021Quote: Healthy human hiPSC cell lines for Figure 4 were cultured under feeder-free conditions on plates coated with human-qualified Matrigel (BD Biosciences) diluted 1:40 in mTeSR-1 medium (StemCell Technologies) ...
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bioRxiv - Immunology 2022Quote: ... Mouse and rat anti-human monoclonal antibodies (mAbs) used in this study included: IFNγ FITC (clone 25723.11, BD Biosciences, cat# 340449), TCRαβ Percp 710 (clone IP26 ...
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bioRxiv - Cancer Biology 2022Quote: The fraction of CD44+/CD24− cells was determined by trypsinizing and resuspending cells in FACS buffer at 10×106 cells/mL followed by incubation with antibodies against human CD44 (APC-conjugated, Clone G44-26, 1:5, BD Biosciences) and CD24 (PE-Cy7-conjugated ...
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bioRxiv - Cancer Biology 2022Quote: ... and engraftment of human cells in the bone marrow and spleen was measured with flow cytometry (CD45-PE-Cy7 antibody, BD Biosciences). Single-cell suspensions were prepared from the bone marrow ...
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bioRxiv - Cancer Biology 2022Quote: ... Isolation of CD4+ T cells was carried out by negative selection using the Human CD4 T Lymphocyte Enrichment Set-DM (BD Biosciences). All patient samples were obtained with informed consent for research use ...
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bioRxiv - Cancer Biology 2022Quote: ... Primary AML cells were harvested and stained with human CD45 and cells were analyzed by a high-throughput automated plate reader (BD LSRFortessa). Dead cells were excluded using the Near-IR Live/Dead marker (Invitrogen) ...
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bioRxiv - Immunology 2022Quote: Human and Non-human primate samples were processed similarly: Blood was collected in 8 mL sodium citrate-containing CPT tubes (BD Biosciences) and separated into plasma and peripheral blood mononuclear cells (PBMC ...