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Citations for Becton, Dickinson and Company :
1151 - 1200 of 7878 citations for Prostaglandin E2 PGE2 Multi Format ELISA Kit 1 Strip Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... for 30 minutes at 4°C and sorted into 384-wells SORTseq plates using a FACS Aria Cell Sorter (BD Biosciences) and directly stored at −80°C until further processing.
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bioRxiv - Cell Biology 2020Quote: ... cells expressing GFP were sorted as single cells in 96-well plates using the 488 nm laser on FACSAria IIu (BD Biosciences). Clones were then analyzed for the expression of target BBS proteins by immunoblotting and sequencing of DNA surrounding the sgRNA target site.
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bioRxiv - Immunology 2021Quote: ... MLN cell suspensions were added to a 96 well plate for surface staining with anti-mouse TCRβ-FITC (clone H57-597, BD Biosciences) and anti-CD4-PerCP-Cy5.5 (clone RM4-5 ...
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bioRxiv - Immunology 2021Quote: ... Single cells were index sorted into 384 well plates containing 1uL of reverse transcription (RT) master mix using a FACS Aria III with FACS Diva software (BD Biosciences) to maintain protein expression with plate well location ...
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bioRxiv - Cell Biology 2020Quote: ... or cell carrier 96 wells plates were coated with a thick layer (150-200uL/cm2) of a mix of growth factor-reduced (GFR) Matrigel (BD Biosciences) and neutralized Bovine Collagen I (Purecol ...
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bioRxiv - Molecular Biology 2021Quote: ... and panned over prewashed 100-mm tissue culture plates coated with CD45 and CD16/32 antibodies (BD Biosciences, San Jose, CA). After incubation for 60 min at 37°C in a 5% CO2 atmosphere to promote adherence of contaminating macrophages and fibroblasts ...
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bioRxiv - Cell Biology 2022Quote: Transduced cells were selected with 10 µg/mL Blasticidin (Thermo) and thereafter sorted in 96 well-plates with a BD FACS Aria III (BD Biosciences) (Imaging Facility ...
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bioRxiv - Immunology 2022Quote: ... Naive CD4+ T cells were stimulated with plate-bound anti-CD3 (LOT: 4310627, eBioscience) and 1.5 μg/ml soluble CD28 monoclonal antibodies (Cat: 557393, BD biosciences pharmingen) in DMEM medium and cytokines (IL-6 ...
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bioRxiv - Immunology 2020Quote: ... and 14 days post-infection (DPI) was determined by culturing serially diluted mouse lung homogenates on 7H10 agar plates (BD Biosciences) at 37°C for 2-3 weeks ...
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bioRxiv - Immunology 2019Quote: ... Plates were washed twice with PBS-Tween and twice with 1x PBS before being developed with OptEIA TMB Substrate (BD Biosciences). Reaction was quenched with 2N hydrochloric acid and plates were read at 450nm ...
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bioRxiv - Neuroscience 2019Quote: ... following which cells were dissociated and cultured in 24-well plates on poly-L-ornithine and Matrigel (BD Biosciences, Bedford, MA) coated glass coverslips in neural differentiation media containing neural growth factors 1x B27 supplement (Life Technologies) ...
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bioRxiv - Immunology 2019Quote: ... were sorted directly into U-bottom 96-well plates containing cell culture media using an Aria III cell sorter (BD Biosciences). For each cell type we sorted 4 single cells ...
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bioRxiv - Biochemistry 2019Quote: ... Stable cells expressing GFP-fusion proteins were trypsinized and single GFP+cells with scatter properties (FSC and SSC) similar to their non-transfected counterparts were plated into 96-wells plates by FACS (Influx Cell Sorter, BD Biosciences). A GFP-negative gate was chosen to select monoclonal stable cells expressing non-fluorescent proteins ...
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bioRxiv - Neuroscience 2019Quote: ... iPSC were treated with Accutase and plated at a density of 50 × 103 cells/well of a 24-wells plate on top of a Matrigel (BD Biosciences) coated coverslip in mTeSR containing Y27632 10 μM (Miltenyibiotec) ...
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bioRxiv - Genetics 2019Quote: ... in PBS + 2.5% FBS and viable single cells were sorted and deposited into the MS-5 seeded 96 well plate using the FACSAria II (BD Biosciences). Single cells were cultured for 16-17 days with an addition of 100μl erythro-myeloid differentiation media per well at day 8.
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bioRxiv - Neuroscience 2019Quote: ... The larvae were placed in the middle of the testing plate made of a petri dish (BD Falcon BioDishXL, BD Biosciences) of size 24.5 x 24.5 cm that was filled with 2% agarose (Agarose Standard ...
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bioRxiv - Neuroscience 2019Quote: ... The larvae were placed in the middle of the testing plate made of a petri dish (BD Falcon BioDishXL, BD Biosciences) of size 24.5 x 24.5 cm that was filled with 2% agarose (Agarose Standard ...
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bioRxiv - Pathology 2019Quote: ... were added in triplicate for each experimental group to the 96-well plates pre-coated with 3 μg/ml anti-CD3 antibody (clone HIT3a, BD Biosciences) for 3-5 h at 37 °C ...
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bioRxiv - Immunology 2021Quote: Post-culture cells were pelleted in V-bottom 96-well plates and resuspended in 30 μl of antibodies at pre-optimized concentrations and diluted in Brilliant Stain Buffer (BD Biosciences), then incubated for 15’ at RT ...
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bioRxiv - Microbiology 2021Quote: ... Single colonies from each recovery plate were picked and used to inoculate a 2ml liquid culture of Nutrient Broth (BD Biosciences). Liquid regrowth cultures (R1 ...
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bioRxiv - Microbiology 2021Quote: ... The cultivars were isolated as single colonies on Tryptic Soy Agar (TSA) plates (30g/L of Tryptic Soy Broth from Sigma Aldrich and 15g/L of Bacto Agar from BD) grown at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... Cells were seeded in 96-well plates (Falcon by Becton–Dickinson Labware Europe, France) coated with collagen I (BD Bioscience, USA), such that a single cell layer homogenously covers each well ...
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bioRxiv - Cancer Biology 2020Quote: ... Polyclonal cell populations were then diluted and sorted into 96-well plates using a BD FACS Aria II flow cytometer (BD Biosciences), such that each well contained on average one cell ...
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bioRxiv - Bioengineering 2021Quote: ... H-2Kb-negative GFP-positive cells were sorted into single cells on a 96-well plate using FACSAria Fusion (BD Biosciences) and cultured for 2-3 weeks in D10 ...
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bioRxiv - Bioengineering 2021Quote: ... The PDMS sheet with the air distribution channels and the plate lid with PDMS cover were both plasma-treated using an Electro-Technic Products Corona Plasma treater (Model BD-20AC). They were then bonded to each other ...
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bioRxiv - Molecular Biology 2021Quote: M.tb strains GFP-Erdman (kindly provided by Dr. Marcus Horwitz, UCLA) and H37Rv (ATCC# 25618) were cultured in 7H11 agar plates (BD BBL), supplemented with oleic acid ...
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bioRxiv - Cancer Biology 2020Quote: ... as assessed by PI and Hoechst staining were sorted into 96 wells plates on a BD FacsJAZZ cell sorter (BD Biosciences) and stored in -80C until further analysis ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were plated at a density of 1.0 × 104 cells per well in triplicate in 96-well plates coated with 50 μl of growth factor - reduced Matrigel (BD Biosciences). Plates were incubated overnight at 37° C ...
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bioRxiv - Cell Biology 2022Quote: ... filtered with a 3 μm filter and sorted for YFP expression into a 96 well plate (5 and 10 events; 48 wells each) (FACSARIA III, BD Biosciences). Individual plaques were screened by PCR and all modified loci were sequenced to confirm correct modification ...
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bioRxiv - Bioengineering 2022Quote: ... All cells were transferred to a V bottom 96-well plate to run flow cytometry analysis on LSRFortessa X-20 (BD Bioscience). mScarlet / Clover ratio was calculated as Q2/(Q2+Q4 ...
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bioRxiv - Immunology 2022Quote: ... from bone marrow were isolated from CFA and MDA-LDL+CFA immunized Aicda-Cre+/ki R26tdTom+/ki mice and single-cell sorted into 384-well plates filled with lysis buffer using an Aria III flow cytometric cell sorter and index sort option (BD Biosciences). RNA from single cells was retrotranscribed using random hexamer primers and cDNA was used as template for three independent nested PCRs with barcoded primers to amplify Igh ...
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bioRxiv - Synthetic Biology 2022Quote: ... samples of 1×106 per mL pooled CAR T cells with 2×106 per mL Nalm 6 were centrifuged at 300 × g for 2 min in 96-well flat-bottom plates and incubated in HTCM with 1x Brefeldin-A/GolgiPlug (BDBiosciences #555029), 1x Monensin/GolgiStop (BD Biosciences #554724) ...
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bioRxiv - Genomics 2022Quote: Cells were plated at 5-15K the day prior to transfection in a 96-well plate coated with poly-D-lysine (BD Biocoat). HEK293FT and HepG2 cells were transfected with Lipofectamine 3000 (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then cultured on anti-hamster IgG coated plates in the presence of hamster anti-CD3 epsilon and anti-CD28 antibodies (BD Biosciences), neutralizing anti-IL-4 antibody (eBioscience) ...
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bioRxiv - Bioengineering 2022Quote: ... 2×105 cells were plated in a well of a 12-well plate coated with 80 μg/ml Growth Factor Reduced Matrigel Basement Membrane Matrix (BD Biosciences). One day later ...
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bioRxiv - Molecular Biology 2020Quote: ... the cells were treated with 1 µg/ml doxycycline for 24 h and single GFP+ cells were sorted into 96-well plates on a BD FACSAria UV instrument (BD Biosciences) and grown until colonies formed.
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bioRxiv - Microbiology 2021Quote: ... The concentration of antibody was quantified in plates coated in triplicate with two-fold dilutions of an appropriate purified mouse Ig isotype standard (IgG and IgA; BD Biosciences), starting at 0.5 μg/μl ...
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bioRxiv - Immunology 2020Quote: ... fibroblast-collagen cultures were prepared in Falcon cell culture inserts and placed into Falcon 6 Well Deep Well TC-Treated Polystyrene Plates (BD Biosciences) containing 2×105 fibroblasts in FBS ...
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bioRxiv - Cancer Biology 2021Quote: ... MDA-MB-231 cells (5 × 104 cells per well) were plated in 24-well culture plate and the top chamber (24-well insert; BD Bioscience) were seeded with MCF7 (2 × 104 cells ...
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bioRxiv - Cancer Biology 2021Quote: ... in 96-well round bottom tissue culture plates in R10 media with the presence of Golgi inhibitors monensin and brefeldin A (BD Bioscience) to reach 200μL per well ...
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bioRxiv - Bioengineering 2021Quote: Cells were plated at 5-15K the day prior to transfection in a 96-well plate coated with poly-D-lysine (BD Biocoat). HEK293FT and HepG2 cells were transfected with Lipofectamine 2000 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... 200 μL/well were plated in triplicate into a 96-well plate pre-coated with 50 μL of bacto-agar (BD Biosciences) in PBS1x (0,66% w/v) ...
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bioRxiv - Immunology 2022Quote: ... 0.75 × 105 naïve T cells were cultured for one or two days with plate-bound anti-CD3ε (2 µg/ml, clone 145-2C11; BD Biosciences) in differentiation medium consisting of RPMI1640 supplemented with 1% penicillin/streptomycin ...
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bioRxiv - Immunology 2022Quote: ... and then tetramer+CD8+7-AAD- cells were sorted into 96-well plates (NIPPON Genetics, Cat# 4ti-0770/C) by using an FACS Aria II (BD Biosciences). TCRα and TCRβ cDNA pairs were amplified from single T cells by a one-step multiplex RT-PCR method described in our previous study19 ...
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bioRxiv - Immunology 2022Quote: Igλ-enriched B1-8 cells were cultured for 2-3 days at 4 *105 cells/well in a 12-well tissue culture plate (BD Falcon) and then washed ...
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bioRxiv - Immunology 2022Quote: ... Eventually 200 000 CD8+ T cells from vaccinated animals were seeded with 50 000 ovalbumin pulsed splenocytes on IFNγ ELISpot assay plates (BD Biosciences). The cells were incubated for 48 hours and the plates were developed as described by the manufacturer.
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bioRxiv - Cancer Biology 2019Quote: 24-well plate inserts were coated with Matrigel and cells were plated on the surface of the Matrigel (BD Sciences 356231) in serum free media ...
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bioRxiv - Genetics 2020Quote: Human embryonic stem (hES) H1 cells (WA01, WiCell) were maintained under feeder free conditions on Matrigel-coated plates (#354277, BD Biosiences) in mTeSR™1 ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were seeded into the center 60 wells of a 96 well plate (Trueline) at precise initial cell numbers using Fluorescence Activated Cell Sorting (BD Fusion) plate sorting at single cell precision ...
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bioRxiv - Microbiology 2020Quote: ... Parasites expressing the transfected Cas9-GFP were sorted by flow cytometry into 96 well plates containing HFF monolayers using a BD Influx cell sorter (BD Biosciences). This line was used for complementing pSFP1myc constructs ...