Labshake search
Citations for Becton, Dickinson and Company :
1101 - 1150 of 1415 citations for Pig Histone H3.3 H3F3A ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... MCP-1 and IL-6 cytokine levels were quantified using the Cytometric bead assay Mouse inflammation kit (BD Bioscience) as recommended by the manufacturer and measured by flow cytometry using a FACS Fortessa flow cytometer.
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bioRxiv - Cancer Biology 2022Quote: ... and then fixed using the BD Cytofix/Cytoperm Fixation/Permeabilization Kit (BD Biosciences, 554722; 20 minutes at 4°C). Cells were then washed twice in 1x BD Perm/Wash buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... according to the manufacturer’s procedure and reverse-transcribed into cDNAs using High Capacity cDNA Reverse Transcription Kit (BD Bioscience) from 1 μg total mRNA according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2021Quote: ... and fixed and permeabilised using Fixation/Permeabilization Solution Kit according to the manufacturer’s instructions (BD Biosciences, San Jose, U.S.A.). Cells were stained with a mouse anti-D8 monoclonal antibody AB1.1 (108 ...
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bioRxiv - Cancer Biology 2020Quote: ... all cells were collected including floating cells and TUNEL staining was performed using the APO-DIRECT Kit (BD Pharmingen) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... CD19+ B cells were extracted from each PBMC sample through magnetic separation (BD iMag Negative Isolation Kit, BD, # 558007). Purified B cells were cultured in RPMI 1640 media supplemented with 15% fetal calf serum (FCS ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell cycle assessment was performed after 20 min of labeling with a BrdU-APC Flow kit (#552598, BD Pharmingen), following the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2019Quote: ... IL-17A (eBio17B7) and Foxp3 (FJK-16s) in permeabilization buffer supplied with the BD Cytofix/Cytoperm kit (BD Biosciences) for 1 hour at 4°C ...
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bioRxiv - Immunology 2021Quote: ... and following surface staining cells were fixed and permeabilized according to the manufacturer’s instructions using the BD Cytofix/Cytoperm Plus Kit (BD. For counting the cells AccuCount Fluorecent particles (Spherotech ...
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bioRxiv - Cancer Biology 2021Quote: ... PC3 transfected cells were used for assessment of invasion using a matrigel invasion chamber kit (BD Bioscience, Sparks, MD). The matrigel coated plates were rehydrated in warm DMEM serum-free medium for 2 h at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... and a lineage cocktail with an isotype control (561317) and the Annexin:PE Apoptosis Detection Kit I were purchased from BD Pharmingen (San Diago ...
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bioRxiv - Immunology 2020Quote: ... Cytokine production (IFNγ, TNF, IL-17A, GM-CSF) was also assessed by Cytometric Bead Array (CBA) Kit (BD Biosciences) at day 3 of polarization ...
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bioRxiv - Genomics 2021Quote: Flow cytometry of iPSC-derived MSCs was performed using the BD Biosciences Human MSC Analysis Kit (BD Biosciences 562245), in combination with the Zombie Violet Fixable Viability Kit (BioLegend 423113) ...
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bioRxiv - Systems Biology 2022Quote: Cytokines in citrated plasma samples were quantified using the BD Cytometric Bead Array Mouse inflammation kit (552364, BD Biosciences) according to manufacturer’s instructions and analyzed using a FACSVerse flow cytometer (BD Biosciences) ...
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bioRxiv - Immunology 2024Quote: ... Cytokine production of T cells was evaluated by surface and intracellular staining using Fixation/Permeabilization Solution Kit (BD Biosciences). Background cytokine staining was subtracted ...
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bioRxiv - Immunology 2024Quote: ... the intracellular expression of viral p27 Gag proteins was assessed by flow cytometry with a CytofixCytoperm kit (BD Biosciences). Antibodies used were as follows ...
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bioRxiv - Immunology 2024Quote: ... The pooled sample was resuspended in an ice-cold sample buffer (BD Biosciences, BD Rhapsody Cartridge Reagent Kit, 633731). Isolation of single cells was performed using the single cell capture and cDNA synthesis kit according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: Cell cycle and cell apoptosis assays were performed according to the manufacturer’s instruction (FITC Annexin V Apoptosis Detection Kit I, BD, 556547 ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed with cold 1x Perm/Wash™ buffer (Cytofix/Cytoperm™ fixation and permeabilization kit, BD Biosciences) and incubated in 1x Perm/Wash™ buffer containing the CTLA4 antibody (Table 3 ...
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bioRxiv - Microbiology 2023Quote: ... in 2% FBS in PBS and fixed and permeabilized with BD Cytofix/Cytoperm™ Fixation/Permeabilization Kit (BD Biosciences). Then ...
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bioRxiv - Immunology 2023Quote: ... samples were washed two times with 1X permeabilization and wash buffer from the transcription factor buffer kit (BD Biosciences) and stained with antibodies against IFN-γ ...
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bioRxiv - Cancer Biology 2023Quote: The cell cycle assay was performed with the PI/RNase Staining Buffer Kit (BD Biosciences, San Jose, CA, USA). A total of 3 × 105 cells were seeded into each well of 6-well plates and allowed to attach overnight ...
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bioRxiv - Microbiology 2023Quote: HIV-infected cells for each Jurkat cell lines were permeabilized using the Cytofix/Cytoperm Fixation/Permeabilization Kit (BD Biosciences) and stained intracellularly using PE-conjugated mouse anti-p24 mAb (clone KC57 ...
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bioRxiv - Neuroscience 2023Quote: ... and performed reverse transcription and an exonuclease reaction using the version 1 3’ kit (BD Biosciences, cat. 633733, 633773).
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bioRxiv - Cancer Biology 2023Quote: ... Single-Cell capture was performed with BD Rhapsody as described above using Rhapsody Enhanced Cartridge Kit (BD Biosciences 664887). Reverse transcription from beads and sequencing library production was carried out according to manufacturer’s instructions (BD Biosciences ...
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bioRxiv - Cell Biology 2023Quote: ... for the detection of cleaved Caspase 3 we used the PE active Caspase 3 apoptosis kit (550914, BD Biosciences) and for the detection of Annexin V we used the FITC Annexin V Kit (Immunostep) ...
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bioRxiv - Developmental Biology 2023Quote: ... Collected cells were washed with PBS and fixed and permeabilized using BD Cytofix/Cytoperm Fixation/Permeabilization Kit (BD Biosciences). Cells were stained with Click-iT Plus OPP reaction cocktail for 30 min at room temperature and analyzed by FACS Symphony (BD Biosciences).
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bioRxiv - Immunology 2023Quote: ... cells were fixed and permeabilized following manufacturer protocol using Cytofix/Cytoperm™ Fixation/Permeabilization Kit (BD Biosciences, cat #554714). After centrifugation and washing ...
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bioRxiv - Immunology 2023Quote: ... followed by fixation and permeabilization using the Fixation/Permeabilization buffer kit according to manufacturer’s instructions (BD Biosciences, NJ, USA). This was followed by staining with anti-phospho-Akt antibody diluted in Perm/Wash buffer for 1h ...
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bioRxiv - Neuroscience 2023Quote: ... astrocytes and endothelial cells (LIVE/DEAD Fixable Aqua Dead Cell Stain Kit, ThermoFisher; CD45.1 clone A20 in BV421 (BD); CD45.2 clone 104 in BV421 (BD) ...
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bioRxiv - Cell Biology 2024Quote: ... bone marrow cells were stained using BD Stemflow™ Mouse Hematopoietic Stem and Progenitor Cell Isolation Kit (BD, 560492) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... BD Rhapsody beads (“Enhanced Cell Capture Beads V2”, Part Number 700034960, BD RhapsodyTM Enhanced Cartridge Reagent Kit, BD 664887) were incubated with a splint ...
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bioRxiv - Immunology 2024Quote: ... Serum supernatant was collected and loaded onto BD Cytometric Bead Array Mouse Th1/Th2/Th17 CBA kit (BD Biosciences), as per manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... co-culture supernatants were thawed at room temperature for 1 hr and cytokines stained with mouse Th1/Th2/Th17 cytokine kit (BD) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Cell quantities were quantified with flow cytometry using the BD Cell Viability Kit with BD Liquid Counting Beads (BD Biosciences). Cultures were then centrifuged at 3,000 g for 15 minutes and washed with sterile PBS three times before dilution into sterile water to a final concentration of 9x105 cfu/mL.
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bioRxiv - Immunology 2021Quote: ... fixed and stained with APC anti-BrdU antibody and 7-AAD using the APC BrdU Flow kit (BD Pharmingen, 552598) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... and IL-17A) were simultaneously measured using the mouse Th1/Th2/Th17 cytokine cytometric bead array (CBA) kit (BD Biosciences). Briefly ...
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bioRxiv - Cancer Biology 2019Quote: Apoptosis of the CTAC cells were determine by using FITC-Annexin V Apoptosis Detection kit (BD Pharmingen, San Diego, CA). The cells were treated with 5 μM of plumbagin for 18 h at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... Single cells were sorted into the 96-well plates of the Precise WTA Kit with predispensed library chemistry using a BD FACSAriaII SORP (BD) and a gating strategy as displayed in Supplementary Figure 3 ...
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bioRxiv - Microbiology 2019Quote: Cytokines were measured in culture supernatants using Cytometric Bead Array (CBA) Mouse Inflammation Kit (BD Bioscience, San Jose, CA, USA). The kit was used for the simultaneous detection of mouse monocyte chemoattractant protein-1 (MCP-1) ...
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bioRxiv - Immunology 2019Quote: Infection-inflicted early cell death was quantified by FACS analyses subsequent to staining of MEFs with FITC/Annexin-V and PI using apoptosis detection kit from BD Biosciences (# 556547) ...
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bioRxiv - Cancer Biology 2019Quote: ... the apoptosis of PANC-1 cells was determined by using the Annexin V-FITC/PI apoptosis detection kit (BD Biosciences). The cells were harvested with trypsin-EDTA (Gibco ...
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bioRxiv - Bioengineering 2020Quote: ... primary human T cells were heated in the thermal cycler prior to assaying with an apoptosis detection kit (BD # 556547) or CellTrace Violet (Fisher # C34571) ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were washed twice with ice-cold PBS and FITC-annexin-PI was done as per manufacturer’s instruction using FITC Annexin V Apoptosis Detection Kit I (Cat. 556547, BD Biosciences). Flow cytometry was performed using a BD LSR II flow cytometer of the Flow Cytometry Core Facility at The Bloomberg School of Public Health ...
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bioRxiv - Microbiology 2020Quote: ... washed twice with cold PBS and then re-suspended in 1x binding buffer(Apoptosis detection Kit I # 556578, BD Pharmingen) at 1x 106 cells/ml ...
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bioRxiv - Immunology 2021Quote: ... lung single cell suspensions were stained with tetramer prior to the surface and intracellular staining for 1 hour at 37°C.Intracellular cytokine staining was performed using the BD Cytofix/Cytoperm kit (BD Biosciences) following manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... buffer (PBS containing 0.1% sodium azide and 1% bovine serum albumin) and fixed/permeabilized using a Cytofix/Cytoperm Buffer Kit (BD Biosciences) or a FoxP3 Transcription Factor Buffer Kit (eBioscience) ...
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Naked Mole-Rat Hematopoietic Stem and Progenitors are Highly Quiescent with an Inherent Myeloid BiasbioRxiv - Cell Biology 2021Quote: ... Cells were washed with Perm/Wash and stained with 1µl/1mio cells anti-BrdU from the FITC BrdU Flow Kit (BD Biosciences) for 20min at RT in the dark ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were stained with the fixable LIVE/DEADTM yellow stain according to the manufacturer’s protocol followed by fixation using the Fixation/Permeabilization kit (BD Biosciences). After fixation ...
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bioRxiv - Cell Biology 2021Quote: ... conjugated anti-c-kit and Peridinin Chlorophyll Protein Complex (PerCP) or allophycocyanin (APC) conjugated anti-CD45 antibodies were purchased from BD Biosciences Pharmingen ...