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Citations for Becton, Dickinson and Company :
1051 - 1100 of 2089 citations for Recombinant Human SELPLG Fc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Pellets were resuspended in 300µL of PBS + 1% v/v FBS and 150µL were added to 2µL of Mouse BD Fc Block (BD Biosciences 553142). Homogenates in Fc block were incubated for 15 minutes at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Single cell suspensions for myeloid panel staining were incubated in Fc Block (Cat#553142, BD Biosciences, Franklin Lakes, NJ, USA). Fluorescent minus one staining controls were used for both panels where required ...
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bioRxiv - Immunology 2022Quote: ... Fc-blocked (1 μg/mL; eBiosciences) BALF cells were stained with anti-mouse SiglecF-PE (0.3 μg/mL; BD Pharmingen), CD11c-APC (0.3 μg/mL ...
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bioRxiv - Immunology 2022Quote: For RNAseq analysis BM cells were isolated by flushing the long bones with FACS buffer (2mM EDTA, 1% FCS, PBS) and treated with Pharm LyseTM buffer (BD). Cells were enriched by magnetic removal of CD11b+ and CD19+ cells (EasySepTM ...
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bioRxiv - Microbiology 2022Quote: ... For each sample 1.5 x 106 cells first underwent Fc receptor blockade with rat anti-mouse FcγRIII/II receptor (CD16/32; BD Biosciences). After Fc receptor blocking for 5 minutes on ice ...
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bioRxiv - Immunology 2022Quote: Ex vivo isolated T cells and in vitro-cultured T cells were incubated with Fc-block (1:250; BD Biosciences) followed by surface staining ...
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bioRxiv - Cancer Biology 2022Quote: ... Mouse cells were incubated with mouse BD Fc BlockTM containing purified anti-mouse CD16/CD32 mAb 2.4G2 at 1:400 (553142, BD BioSciences) for 10 min at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Isolated intestinal epithelial cells (107 cells/1ml) were incubated with Mouse BD Fc Block™ (55314, BD, Franklin Lakes, NJ) for 10 minutes on ice in IESC media (DMEM/F12 ...
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bioRxiv - Microbiology 2020Quote: ... and 3×106 cells per tube were stained as follows: cells were incubated in 0.5 μg Fc block (BD Biosciences) for 15 min at 4°C in staining buffer (Ca2+ - and Mg2+ - free PBS containing 3% heat-inactivated fetal bovine serum ...
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bioRxiv - Microbiology 2021Quote: ... Fc receptors were blocked using the anti-mouse CD16/CD32 (FcBlock clone 2.4G2) monoclonal antibodies (BD Biosciences, #553141, 1/400) and cells were stained for 30 min at 4°C with a combination of the following anti mouse immunophenotyping antibodies ...
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bioRxiv - Cancer Biology 2021Quote: ... Single-cell suspensions were stained with 5 μM Cell-ID Cisplatin (Fluidigm Corp.) and incubated with Fc block (BD Biosciences), followed by surface antibody cocktail ...
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bioRxiv - Neuroscience 2022Quote: ... Unspecific binding of antibodies was blocked by previous incubation for 10 min with anti CD16/CD32 (Fc block, clone 2.4G2; #55314, BD Pharmingen) in Fluorescence Activated Cell Sorting (FACS ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed and incubated (15-20 min., on ice) with Fc Block (1:100 in 1% FBS/PBS, Clone 2.4G2, BD Biosciences), followed by anti-CD45-PE-Cy5.5 (1:100 ...
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bioRxiv - Cell Biology 2020Quote: ... 3×106 cells were incubated with purified rat IgG2b anti-mouse CD16/32 receptor antibody as per supplier’s instructions (Mouse BD Fc-block, clone 2.4G2, BD Biosciences, 553141) for 20 minutes at 4°C ...
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Single cell transcriptomics reveals the effect of PD-L1/TGF-β blockade on the tumor microenvironmentbioRxiv - Genomics 2020Quote: ... The liquid was poured off from the cells (leaving approximately 100 µl in each tube) and 5 µl of mouse Fc Blocking Reagent (rat anti-Mouse CD16/CD32 BD) was added to samples and incubated for 5 minutes at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed in FACS buffer (PBS with 2% Heat-inactivated FCS) and fixed in 1x BD cellFIX (BD Biosciences). Samples were acquired on the LSRFortessa (BD Biosciences) ...
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bioRxiv - Immunology 2021Quote: Surface staining was done in the presence of anti-CD16/32 antibodies to block FcγRII/III receptors using Mouse Fc blockTM (BD Biosciences). Cells were incubated with the Mouse Fc blockTM for 10 min followed by incubation with fluorochrome-conjugated pre-titrated antibodies for 20 min at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Pellets were suspended in 100 μl of flow buffer and blocked with 0.25 μg Fc block (BD Pharmingen, CA, USA) for 10 min followed by staining with antibodies ...
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bioRxiv - Immunology 2020Quote: ... Samples were then washed with 2% and stained with 2% FCS in PBS and subsequently washed stained with goat anti-rabbit IgG –FITC secondary (BD) for 30 minutes at RT ...
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bioRxiv - Neuroscience 2019Quote: Cell pellets were eluted in FACs buffer (0.5% BSA in PBS with 2mM EDTA) and blocked for ten min with Fc preblock (CD16/CD32, BD 553141), followed by 20 minute staining with anti-CD31-APC (1:100 ...
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bioRxiv - Cell Biology 2020Quote: ... cells were resuspended in PBS with 1% FCS and analyzed for GFP and AF647 fluorescence on a FACS Verse (BD). Nontransfected and non-AF647-stained cells were used for gating the cells positive for GFP and AF647 ...
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bioRxiv - Immunology 2020Quote: ... Non-specific binding was blocked with 3% (w/v) bovine serum albumin (BSA, ChemCruz) in PBS with 0.5 g/mL Fc Block (BD Biosciences) for 30 minutes ...
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bioRxiv - Immunology 2021Quote: ... All samples were then incubated with Fc block (5 μg mL-1 CD16/CD32 (BD Biosciences, San Diego, CA, USA) and 0.1 % mouse serum in FACs buffer (PBS containing 0.5 % BSA and 2 mM EDTA (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... Cells were then treated with dasatinib for 1 hr at 37°C prior to blocking with Fc-block (BD Bioscience), 5 % normal mouse serum ...
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bioRxiv - Immunology 2021Quote: ... Surface antibody cocktail was prepared in cell staining buffer (Fluidigm) and added to all samples equally after Fc block (BD). Cells were washed then fixed and permeabilized using eBioscience Transcription Factor Fix/Perm Buffer followed by barcoding (Fluidigm) ...
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bioRxiv - Genomics 2021Quote: ... Cell counting was performed by counting of the events with FCS ≥ 1.104 and SSC ≥ 5.105 by flow cytometry (BD-Accuri-C6). The transposition activity was represented as the ratio of the hygromycine-resistant colonies to the total cells ...
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bioRxiv - Immunology 2022Quote: Single cell suspensions were first incubated for 10 minutes on ice with anti-mouse CD16/32 Fc Block (BD Biosciences), and subsequently stained with fluorophore-conjugated antibodies against surface markers at 4°C for 20 mins ...
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bioRxiv - Cancer Biology 2022Quote: ... The cells were then resuspended incubated with anti-mouse CD16/CD32 at 1:400 (BD Fc BlockTM, BD Biosciences #553142) for 10 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... The cells were then resuspended incubated with anti-mouse CD16/CD32 at 1:400 (BD Fc BlockTM, BD Biosciences #553142) for 10 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were counted and resuspended to 10×106 cells/100μl in 1:50 Rat Anti-mouse CD16/CD32 Moue Fc Block (BD Biosciences) in FACS buffer (3% FBS ...
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bioRxiv - Immunology 2022Quote: ... Red blood cells were lysed using ammonium chloride and Fc receptors were blocked using anti-mouse CD16/CD32 (BD Bioscience) before staining.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and resuspended in 100 μL of a 1:50 dilution of Mouse BD FC block (BD Pharmigen, San Joe, USA). Cells were then stained using the following fluorescent antibodies ...
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bioRxiv - Cancer Biology 2022Quote: ... Single cell suspensions were washed with 1XPBS-4%FBS before incubation for 20 min on ice at 5•107 cells/ml with 500 ng/ml Fc block (2.4G2, BD Pharmingen). Cells were then incubated for 1 hour on ice with AF700 conjugated CD45.2 monoclonal antibody (104 ...
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bioRxiv - Immunology 2024Quote: ... and then stained for 30 min at 4°C in PBA buffer (PBS, 0.5% BSA, 2 mM EDTA) in the presence of Fc Block (1:50 dilution, BD-Biosciences), using the following antibody combination ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cells (1.5 x 106 cells) first underwent Fc receptor blockade with rat anti-mouse FcψRIII/II receptor (CD16/32; BD Biosciences). After blocking for 5 minutes on ice ...
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bioRxiv - Microbiology 2024Quote: ... Samples were blocked for 30 minutes in FACS buffer (2% FBS 1X PBS) containing Fc Block anti-CD16/32 (2.4G2, BD Biosciences) (1:100 dilution) ...
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bioRxiv - Immunology 2023Quote: ... Surface antigens were stained for 30 min at 4°C with indicated antibodies (Table.1) after Fc blocking for 4°C 30 min with anti-mouse CD16/32 (BD) without wash ...
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bioRxiv - Genetics 2023Quote: ... 10 × 106 bone marrow cells were resuspended in 200 μl of PBS supplemented with 2 % FCS containing the following antibody solution: FITC-conjugated lineage cocktail with antibodies against CD4 (clone H129.19, BD Pharmingen), CD3e (clone 145-2C11 ...
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bioRxiv - Immunology 2022Quote: ... Fc blocking was performed with a combination of antibodies diluted in FACS buffer with Brilliant Violet Stain Buffer (BD Biosciences) for 30 minutes at 4°C in the dark ...
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bioRxiv - Immunology 2023Quote: ... Single cell suspensions were incubated for 15 minutes at 4°C with anti CD16/CD32 Mouse BD Fc Block diluted 1/50 (BD) in 100 µL of FACS buffer in 96 wells round-bottom plates (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2023Quote: ... the stained cells were stored in DPBS supplemented with 2.5% (v/v) paraformaldehyde (PFA) and 1% FCS before flow cytometry measurement on a BD LSRFortessa flow cytometer (BD Biosciences). pMHC-I multimer binding T cells were identified by a Boolean gating strategy in FlowJo (BD Biosciences ...
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bioRxiv - Cell Biology 2022Quote: ... The eluted macrophage fraction was resuspended in a final volume of 200μl MACS buffer and incubated 10 min with Fc block (1:50, BD Pharmingen). 200μl of staining solution containing flow cytometry antibodies (anti-CD45-PECy7 (1:100 ...
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bioRxiv - Cancer Biology 2023Quote: Spleens and livers were harvested into ice cold FACS buffer (PBS supplemented with 2% FCS v/v) and then emulsified through a 70μM filter (BD Biosciences), centrifuged and washed in FACS buffer ...
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bioRxiv - Developmental Biology 2023Quote: ... one million cells were transferred in 100 μl of FACS buffer supplemented with 0.5μM Y27632 and then added 2 μl Fc Block (BD Pharmingen, 553141) per sample followed by 10 min incubation on ice ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed and resuspended in PBS + 3% FCS for cell sorting on the BD FACS Aria Fusion (BD Biosciences) at the NIHR Cambridge BRC Cell Phenotyping hub ...
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bioRxiv - Cancer Biology 2023Quote: ... Each sample was Fc blocked before staining using 50 µL of 2X FC Block Solution (FACS Buffer, 1:125 dilution of anti-CD16/CD32 (BD) and incubated for 10 minutes on ice ...
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bioRxiv - Cancer Biology 2023Quote: ... The binding of IGSF3-Fc to the cell surface was detected as the APC fluorescence intensity using FACSVerse (BD Biosciences).
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bioRxiv - Systems Biology 2023Quote: All flow cytometry raw data were saved as .fcs files and processed by the FlowJo software (version 10, BD Biosciences) for further analysis ...
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bioRxiv - Cancer Biology 2022Quote: ... IL-2 and IFNγ cytokine concentrations were analyzed by sandwich ELISA according to manufacturer’s kit protocol (BD Opteia human IL-2 and human IFNγ). In vivo cerebellum lysates were analyzed for cytokines similarly ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were stained with Alexa Fluor 647–conjugated anti– human CD24 and APC-H7 labeled anti–human CD44 antibodies (BD Pharmingen, San Jose, CA) at 4°C for 20 min ...