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Citations for Becton, Dickinson and Company :
1051 - 1100 of 1347 citations for NKG2DL2 Human HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: Murine organs and human LNs (1 cm3 or smaller in size) were fixed with BD CytoFix/CytoPerm (BD Biosciences) diluted in PBS (1:4 ...
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bioRxiv - Immunology 2020Quote: Fluorophore or biotin-conjugated antibodies with specificity to human cell surface antigens and cytokines were purchased mainly from BD Biosciences or Thermo Fisher Scientific and in some occasions from other suppliers as indicated below ...
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bioRxiv - Immunology 2020Quote: ... Cells were seeded (30,000-50,000/wells) into wells pre-coated with anti-human CD3 (2 μg/ml, clone UCHT1, BD Biosciences) along with soluble anti-human CD28 (1 μg/ml ...
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bioRxiv - Pathology 2021Quote: ... and the resulting single-cell suspension was labeled with Phycoerythrin (PE)-conjugated mouse anti-human CD146 antibody (BD Biosciences) or CD31 antibody (Abcam ...
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bioRxiv - Cancer Biology 2020Quote: ... The viability and purity of the neutrophils were determined by anti-human CD66b PE (clone G10F5; BD Biosciences, 561650) and anti-human CD16 FITC (clone 3G8 ...
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bioRxiv - Genomics 2021Quote: Roughly 80 ml of human blood was drawn to and kept in spray-coated EDTA tubes (BD Vacutainer #366643) at 4°C ...
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bioRxiv - Immunology 2020Quote: ... peridinin chlorophyll protein complex cyanine (PerCP-Cy™5.5) mouse anti-human CD163 (BD Biosciences; San Jose, CA, USA) and allophycocyanin (APC ...
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bioRxiv - Genomics 2021Quote: Flow cytometry of iPSC-derived MSCs was performed using the BD Biosciences Human MSC Analysis Kit (BD Biosciences 562245), in combination with the Zombie Violet Fixable Viability Kit (BioLegend 423113) ...
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bioRxiv - Cancer Biology 2021Quote: ... dissociated tumor cells were Fc receptor blocked by incubating 1*106 cells/50-100 µl of staining buffer with 2.5 µg of Human Fc Block (Cat # 564220; BD) for 10 minutes.
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bioRxiv - Bioengineering 2022Quote: ... and resuspended in staining buffer containing: 1:100 dilution V450 mouse anti-human CD31 (BD Biosciences, Mississauga, ON, Canada), 1:10 dilution PE mouse anti-human CD34 (BD Biosciences ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were incubated with PE-conjugated mouse monoclonal anti-human IGF-1R antibody (BD Biosciences, Franklin Lakes, NJ) or PE-conjugated isotype-matched control mouse antibody (IgG1k ...
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bioRxiv - Microbiology 2022Quote: ... human serum was isolated from venous blood of the same donors taken into serum separation blood collection tubes (BD Vacutainer ...
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bioRxiv - Cancer Biology 2023Quote: ... to block MHC-I and 30 μg/mL mouse anti-human HLA-DR/DP/DQ (clone Tu39, BD Biosciences) to block MHC-II for 30 minutes at 37 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... Th2 cells were re-activated using 1μg/mL αCD3 (Purified NA/LE Mouse anti-Human CD3, 555329, BD Pharmingen) and 1μg/mL αCD28 (Purified NA/LE Mouse anti-Human CD28 ...
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bioRxiv - Neuroscience 2022Quote: iPSC lines were cultured on plates coated with human-qualified Matrigel (BD Biosciences; dilution 1:40 in DMEM-F12) in mTeSR (StemCell Technologies ...
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bioRxiv - Biophysics 2024Quote: ... Interferon gamma production was assessed by intracellular staining using mouse anti-human IFN-g FITC (BD Bioscience, clone 25723.11) and BD Cytofix/Cytoperm (BD Bioscience ...
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bioRxiv - Genomics 2024Quote: Human induced pluripotent stem cells (iPSCs) were maintained in mTeSR medium on plates pre-treated with Matrigel from BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... they were incubated for 10 min at room temperature with a combined Fc block solution (2μL Human Fc block [BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... Cells were seeded (30,000-50,000/well) into wells pre-coated with anti-human CD3 (2 μg/ml, clone UCHT1, BD Biosciences) along with soluble anti-human CD28 (1 μg/ml ...
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bioRxiv - Biochemistry 2023Quote: ... 50 μL of 1:250 mouse anti-human CD15 clone HI98 [anti-Lewis x] (BD Biosciences, catalog no. 555400BD) or mouse-anti-human blood group Lewis a clone 7LE (Abcam ...
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bioRxiv - Cancer Biology 2023Quote: ... Surface or total MPL proteins were stained with anti- human MPL-PE antibody (BD Pharmingen 562159; 1:50 dilution).
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bioRxiv - Cancer Biology 2023Quote: ... and stained with the following antibodies: anti-human CD45-APC (clone HI30, #555485, BD Bioscience, Franklin Lakes, NJ, USA) (hCD45 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... cryopreserved human hepatocytes (Lot BD195: 2-year-old, Hispanic female, 1 × 106 hepatocytes, BD Biosciences, Ann Arbor, MI, USA) were transplanted under anesthesia into 2-to 4-week-old hemizygous cDNA-uPA/SCID mice through the spleen ...
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bioRxiv - Immunology 2023Quote: ... 100,000 PBMC per well were seeded in complete media (Advanced RPMI with 10% human serum and 1% L-Glutamine) supplemented with 1µg/mL of anti-CD28 antibody (Clone #37.51, BD Biosciences). The L598 compound was added to the cultures every two days at 2 ...
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bioRxiv - Cell Biology 2023Quote: ... mouse monoclonal antibody against human CD49e/Integrin α5 Chain (1:1000 for western blotting; BD Pharmingen; catalogue number 555614); mouse monoclonal antibody against human Integrin alpha-5 (1:100 for immunofluorescence ...
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bioRxiv - Biophysics 2023Quote: Monoclonal mouse anti-human CD44 (Clone G44-26) and monoclonal mouse anti-CD209 (Clone DCN46) were obtained from BD Biosciences ...
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bioRxiv - Molecular Biology 2023Quote: ... GPIIIa, Luc.H11-FITC, #M031-1 or Integrin ⍺ 5/CD49e, Tap.A12-FITC and anti-human CD61-PE, #555754, BD Biosciences) and incubated for 15 min at RT in the dark ...
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bioRxiv - Biophysics 2024Quote: ... to a final concentration of 0.4 mg ml-1 for DHFR or 0.1 mg ml-1 for human fibronectin (BD Biosciences), with a total volume of 100 µl in a 96-well plate ...
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bioRxiv - Cell Biology 2024Quote: ... the levels of secreted cytokines were quantified using the BD OptEIA™ Human IL-6 ELISA kit (BD Biosciences). Cells were trypsinized and counted for normalization of cytokine levels per cell.
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bioRxiv - Cancer Biology 2021Quote: ... Antibodies used are as follows and were used according to manufacturer’s protocol: PE-Cy7 Mouse Anti-Human CD3 (Cat 563423; BD Pharmingen), PE Mouse Anti-Human CD4 (Cat555347 ...
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bioRxiv - Systems Biology 2020Quote: ... Cells were stained at 4°C for 15 minutes with an APC-anti-Human-CD62L (clone DREG-56, BD Pharmingen), a BV650-anti-Human-CD11b (BioLegend ...
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bioRxiv - Immunology 2020Quote: ... 2mM EDTA and 0.05% NaN3): PerCP-Cy™5.5 mouse anti-human CD19 (1:40 dilution, Clone HIB19, BD Pharmingen) (for PBMC samples only) ...
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bioRxiv - Immunology 2022Quote: ... the resulting bulk transduced population positive for Human ACE2 expression stained by FITC-anti-ACE2 Antibody (Sinobiological) was sorted by FACS Aria SORP (BD) and maintained in the culture medium in the presence of 2 μg/ml of puromycin.
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bioRxiv - Bioengineering 2019Quote: ... the monocytes were incubated for 10 min at room temperature with human BD Fc-block (2.5 µg per 1×106 cells in 100 µL, Cat. no. 564220, BD biosciences). The cells were further incubated with the following antibody cocktail for 30 min at 4°C ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were then washed twice 5 min in TBST and incubated with the primary antibody over night at 4°C (human and mouse a-syn: 610786 BD Biosciences ...
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bioRxiv - Systems Biology 2019Quote: Total T cells were isolated from peripheral blood mononuclear cells from 3 human donors via negative selection (Stem Cell Technologie cat. no. 19051) and plated in anti-CD3 (BD Pharmingen clone UCHT1 ...
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bioRxiv - Immunology 2021Quote: ... 1 mM EDTA) with the following anti-human antibodies (all at 1:200 dilution): anti-CD20-PECy7 (BD Biosciences, 335793), anti-CD3-APC-eFluro 780 (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... Phenotyping BCAMhi and BCAMlo organoids: Single cells were incubated with anti-human BCAM BV421 (clone B64; 1:50; BD Biosciences), EGFR BV605 ...
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bioRxiv - Cell Biology 2021Quote: Human THP-1 cells (NCTC #88081201) were maintained or passaged in 25 or 75 cm2 tissue culture flasks (BD Biosciences) using RPMI-1640 (Lonza ...
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bioRxiv - Bioengineering 2020Quote: ... primary human T cells were heated in the thermal cycler prior to assaying with an apoptosis detection kit (BD # 556547) or CellTrace Violet (Fisher # C34571) ...
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bioRxiv - Bioengineering 2021Quote: ... live/dead stain in PBS for 15 min on ice before washing and re-suspending in 2x human/mouse Fc block in stain buffer (BD) followed by surface-staining with monomeric GFP and anti-human CD45 AF700 (Biolegend clone HI30) ...
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bioRxiv - Genomics 2020Quote: Primary human bronchial epithelial basal cells (Passage 1 or 2) were expanded on flasks coated with collagen I (BD Biocoat) in bronchial epithelial growth medium (BEGM) ...
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bioRxiv - Genomics 2020Quote: ... Primary antibodies (anti-Cas9 7A9-3A3, Cell Signaling Technology #14697S, anti-γ-tubulin Sigma#T5326, anti-Human Retinoblastoma protein BD Pharmigen #554136 ...
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bioRxiv - Immunology 2022Quote: ... Pellets were resuspended in 50 µL cold PBS 0.25% BSA with PE-conjugated mouse anti-human IgG (1:100, BD Biosciences), or PE-conjugated goat-anti human IgA (1:800) ...
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bioRxiv - Immunology 2022Quote: ... B cells were also stained with following anti-human antibodies (all at 1:200 dilution): anti-IgD-BV650 (BD, 740594), anti-CD27-BV786 (BD biosciences ...
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bioRxiv - Immunology 2022Quote: ... 1 mM EDTA) with the following anti-human antibodies (all at 1:200 dilution): anti-CD20-PECy7 (BD Biosciences, 335793), anti-CD3-APC-eFluro 780 (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... Samples were stained with fluorochrome-labeled monoclonal antibodies against mouse and human cell-surface antigens and analyzed on an LSRII flow cytometer (BDBiosciences). Data were analyzed using FloJo software.
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bioRxiv - Immunology 2020Quote: ... APC mouse anti-human CD184 (clone 12G5; #555976) and PE rat anti-human CD184 (clone 1D9; #551510) and the appropriate isotype controls were obtained from BD Pharmingen ™ ...
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bioRxiv - Microbiology 2020Quote: ... Samples were taken from the basolateral medium over time and the concentration of chemokines and cytokines was determined using the LEGENDplex Human Anti-Virus Response Panel (13-plex) and the LEGENDplex Human Proinflammatory Chemokine Panel (13-plex) on a FACSCanto II flow cytometer (BD). Samples were run in duplicate and measured at two different dilutions.
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bioRxiv - Cell Biology 2021Quote: ... platelets in ACD-A anticoagulated whole blood were stained with FITC-labelled anti-human CD61 antibody (Clone: RUU-PL7F12, Cat No: 340715 BD Pharmingen ...