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Citations for Becton, Dickinson and Company :
1001 - 1050 of 2473 citations for Recombinant Human MSLN Protein Fc Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: The following anti-mouse antibodies were used following manufacturer’s instructions: anti-CD16/CD32 Fc blocking antibody (BD Biosciences), anti-CD24-FITC (clone M1/69 ...
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bioRxiv - Immunology 2022Quote: ... and were resuspended in FC stain buffer prior to data collection via an LSR Fortessa flow cytometer (BD). Analysis was done using FlowJo software (version 10.7.1) ...
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bioRxiv - Neuroscience 2023Quote: ... Fc receptors were blocked with 100 mL solution containing anti-mouse CD16/CD32 (1:100, BD Pharmingen, 553141) for 30 minutes at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Fc receptors were blocked with anti-CD16/32 in the antibody staining solutions (BD Biosciences, Franklin Lakes, NJ). For RPE and endothelial identification ...
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bioRxiv - Microbiology 2022Quote: ... Single cell suspensions stained in PBS + 2% FBS in the presence of Fc receptor blocking antibody (BD Pharmingen) and stained with the antibodies against the following mouse proteins ...
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bioRxiv - Immunology 2023Quote: ... Monocytes were sorted into HBSS-2% FCS in 15ml Falcon tubes using a BD Aria III (BD Biosciences). Monocytes were gated as Lineage-negative (B220 ...
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bioRxiv - Immunology 2023Quote: ... Cells were stained for 45 min on ice in FC buffer containing unlabelled CD32 (BD Biosciences, Sydney, Australia) to block Fc receptor binding ...
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bioRxiv - Microbiology 2023Quote: ... and Fixable Viability Dye eFluor 780 (Thermo Fischer) in presence of FC Block (BD, 20 min, 4°C). Stained cells were washed in FACS buffer twice and fixed in 2% paraformaldehyde (30 min ...
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bioRxiv - Immunology 2023Quote: ... Cells were Fc-blocked with anti-mouse CD16/CD32 (1:100, Cat# 553141, BD Biosciences, San Jose, CA) followed by surface marker staining with antibodies to MerTK (2B10C42 ...
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bioRxiv - Immunology 2023Quote: ... and incubated for another 15 minutes at 4°C in the presence of Fc receptor block (BD Biosciences). Antibody cocktails for surface proteins were prepared in PBS + 2% FBS and Brilliant Stain Buffer (50 µl/100 µl of cocktail ...
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bioRxiv - Cell Biology 2023Quote: FC analyses of SkM single cell suspensions were performed with the cell analyser LSRFortessa X-20 (BD Bioscience) using the FACSDiva 8.0 software ...
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bioRxiv - Immunology 2024Quote: ... 2mM EDTA) and incubated with Mouse BD Fc Block purified anti-mouse CD16/CD32 mAb 2.4G2 (BD Biosciences) to block non-specific staining ...
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bioRxiv - Immunology 2024Quote: The FCS files of CyTOF were analyzed with dimensionality reduction and clustering approaches using FlowJo-10 (BD Biosciences) and its plugins including DownSampleV3 ...
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bioRxiv - Immunology 2021Quote: ... PBMCs and mdDCs culture supernatants were quantified by the Cytometric Bead Array (CBA) method using the Human Inflammatory Cytokines Kit and Human Chemokine Kit (BD Biosciences), following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated with PE-conjugated mouse anti-Human CD44 and mouse anti-Human CD184 antibodies (BD Biosciences, San Jose, CA) at a concentration of 5µl/10 million cells ...
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bioRxiv - Immunology 2020Quote: ... Flow cytometry experiments were performed using PE-anti-human CD54 (ICAM-1) (REA266, Miltenyi) and APC-anti-human CD20 (clone L27, BD Biosciences). Antibodies for blocking LFA-1 - ICAM-1 interaction were anti-ICAM1 (clone HA58 ...
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bioRxiv - Microbiology 2021Quote: ... Purification of human CD34+ cells were assessed by flow cytometry using an anti-human CD34-FITC antibody (clone 581, BD Biosciences). Fetal liver derived from three different donors were used in this study.
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bioRxiv - Immunology 2022Quote: Suspensions of human Tconv cells or Treg cells were stained with V500-conjugated anti-human CD4 (clone RPA-T4, BD Biosciences), PE-Cy7-conjugated anti-human CD25 (clone BC96 ...
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bioRxiv - Genetics 2020Quote: ... and permeabilized using BD Cytofix/Cytoperm kit before incubation with BV605 anti-human IFN-gamma (B27 clone) and BUV395 anti-human TNF-alfa (MAB11 clone, BD Biosciences) in permeabilization buffer at 4°C for 30 min in the dark ...
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bioRxiv - Immunology 2020Quote: ... mouse anti human ICAM-1 (CD54) and mouse anti human VCAM-1 (CD106) antibodies and fixed with FACS lysing solution (BD Bioscience). Surface levels were determined by flow cytometry (Gallios ...
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated with PE-conjugated mouse anti-Human CD44 and mouse anti-Human CD184 antibodies (BD Biosciences, San Jose, CA) at a concentration of 5μL/10 million cells ...
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bioRxiv - Microbiology 2022Quote: ... Housekeeping protein β-actin (a cytoskeleton protein; dilution, 1:5,000; BD Biosciences) was used as loading controls ...
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bioRxiv - Immunology 2021Quote: ... protein transport inhibitor (BD Golgi Plug™ containing Brefeldin A ...
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bioRxiv - Immunology 2021Quote: ... protein transport inhibitor (BD Golgi Plug™ containing Brefeldin A ...
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bioRxiv - Immunology 2021Quote: For TCR stimulation naïve and primed CD8+ T cells were incubated for 20 min at 4°C with biotinylated anti-CD3ε mAb (5 and 1 μg/mL, BD Biosciences #553060). Cells were washed and stimulated for the indicated time by adding streptavidin (20 μg/mL ...
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bioRxiv - Microbiology 2021Quote: ... the same protocol was followed except that of the use of biotinylated anti-Syrian hamster IgG1 /IgG2 antibodies in 1:10000 dilution (BD biosciences, USA) and Streptavidin-horseradish peroxidase 1:8000 (Thermo-scientific ...
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IL-27R signaling serves as immunological checkpoint for NK cells to promote hepatocellular carcinomabioRxiv - Immunology 2020Quote: ... After washing with 1% BSA-PBS slides were incubated with secondary goat anti-rat and goat anti-rabbit biotinylated antibodies for 30 min at RT followed by 30 min of incubation with streptavidin–HRP (1:500; BD Pharmingen, 554066). For developing DAB substrate (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... IFNγ produced upon re-activation of peptide-specific T cells and captured on anti-IFNγ capture antibody was visualized via sequential addition of 2 μg/mL biotinylated anti-IFNy detection antibody (BD #51-1818KA), Streptavidin-Horseradish Peroxidase (HRP ...
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bioRxiv - Immunology 2024Quote: ... Content of chemokines / cytokines was quantified after washing and staining with biotinylated detection antibodies and phycoerythrin bound streptavidin by using LSR II (BD Bioscience, Germany). Calculation of each chemokine / cytokine quantity was determined by using standard curves according manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... Human cytokines expression (hTNFα and hIFNγ) in human T cells were analyzed using BD LSRFortessa™ cell analyzer - flow cytometer (BD Biosciences) and the data were analyzed using FlowJo software (Dako).
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bioRxiv - Microbiology 2023Quote: ... Capture and detection antibodies for human IL-6 were from the OptEIA set for human IL-6 (Cat# 55220, RRID: AB_2869045; BD Biosciences, Heidelberg, Germany) and capture and detection antibodies for human TNF were from the OptEIA set for human TNF (Cat# 555212 ...
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bioRxiv - Microbiology 2023Quote: ... and capture and detection antibodies for human TNF were from the OptEIA set for human TNF (Cat# 555212, RRID: AB_2869042; BD Biosciences, Heidelberg, Germany). Signals were detected by Streptavidin-PE (Agilent ...
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bioRxiv - Cell Biology 2020Quote: ... Antibodies against CD16/32 (Fc block; clone 2.4G2) and ITGB2 (clone CTB104; Alexa Fluor 488 conjugated) were from BD Pharmingen and Santa Cruz Biotechnology Inc. ...
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bioRxiv - Immunology 2022Quote: Flow cytometry of peritoneal cells was performed using anti-mouse CD16/32 (Fc Block, BD Biosciences, San Jose, CA) before staining with primary antibody or isotype controls ...
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bioRxiv - Microbiology 2021Quote: ... 1×106 cells were blocked with mouse BD FC block™ (5 μg/mL, BD Pharmingen, Cat No. 553141) and then stained using fluorescent-labeled monoclonal antibodies as follows ...
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bioRxiv - Cancer Biology 2020Quote: ... for 15 minutes to block Fc receptors and then stained using with antibodies to CD45-APC-Cy7 (BD 557659) and SYTOX Red Dead Cell Stain (Life Technologies S34859 ...
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bioRxiv - Immunology 2020Quote: ... cells were washed in perm/wash solution and resuspended in 0.5% PFA-FC stain buffer prior to running on a Symphony A5 flow cytometer (BD). Analysis was performed using FlowJo software ...
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bioRxiv - Systems Biology 2022Quote: Citrated blood collected from infected and control mice was diluted with HEPES buffer containing mouse Fc-block (BD Pharmingen). Platelets ...
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bioRxiv - Immunology 2019Quote: ... FCS in PBS and were stained for 1 hour at 37°C with Vγ5 TCR-FITC (clone 536; BD), MHCII-AF647 (I-A/IE ...
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bioRxiv - Immunology 2020Quote: ... and 10% (v/v) FCS (from herein referred to as DC culture media. The antibodies were purchased from BD, Miltenyi ...
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bioRxiv - Neuroscience 2023Quote: ... Fc receptors were blocked for 15 min with rat anti-CD16/32 (1:100, catalog no. 553141; BD Biosciences) and cells were washed and labeled with the following antibodies for 30 min at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed and blocked with blocking buffer plus Fc receptor blocking antibody (Clone Fc1.3216, BD Biosciences, United Kingdom). Cells were incubated at RT for 10 min then centrifuged (800 g ...
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bioRxiv - Immunology 2022Quote: Cells were incubated with purified anti-mouse CD16/CD32 clone 2.4G2 (Fc block; BD Biosciences, San Jose, CA, USA), and stained as described with H-2k(d ...
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bioRxiv - Immunology 2024Quote: ... 1% FCS and 2mM EDTA) and stained for extracellular molecules with the following antibodies: anti-CD8a (53-6.7, BD), anti-CD4 (GK1.4 ...
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bioRxiv - Immunology 2024Quote: ... and Fc-receptors were blocked (2.4G2) before staining with monoclonal antibodies before collection on a Fortessa flow cytometer (BD). Fluorochrome-conjugated antibodies to the following cell surface molecules were used ...
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bioRxiv - Immunology 2024Quote: ... cells were blocked with 2% FBS and 0.2% goat serum containing HBSS including 1:1000 Mouse BD Fc Block™ (Clone 2.4G2, BDBiosciences). For detection of endogenous p65 ...
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bioRxiv - Immunology 2024Quote: ... Cells were blocked with 2% FBS and 0.2% goat serum containing HBSS including 1:1000 Mouse BD Fc Block™ (Clone 2.4G2, BDBiosciences) and stained with cell surface marker detecting fluorophore-conjugated antibodies ...
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bioRxiv - Immunology 2024Quote: ... and blocking with 2% FBS and 0.2% goat serum containing HBSS including 1:1000 Mouse BD Fc Block™ (Clone 2.4G2, BDBiosciences). Intracellular proteins were stained with fluorophore-conjugated antibodies overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: Single-cell suspensions from tumors or spleens were incubated in Fc block (BD Biosciences, 553142, vol/vol 1/50) at room temperature for 10 min ...
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bioRxiv - Cell Biology 2023Quote: ... Data collection was done using BD FACSDiva™ software and .fcs files were formally analyzed with FlowJo (FlowJo, BD). All flow cytometry experiments contained single color controls for compensation and gating.