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Citations for Becton, Dickinson and Company :
1001 - 1050 of 4029 citations for IL 1beta Mouse CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: Supernatants were collected 4 or 24 h post-challenge and the levels of TNF and mature IL-1β were quantified by ELISA using OptEIA human TNF ELISA Set (BD Biosciences) and human IL-1β/IL-1F2 DuoSet ELISA (R&D systems) ...
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bioRxiv - Microbiology 2020Quote: Macrophages were infected in a 48-well plate as described above and harvested supernatants were assayed for cytokine levels using ELISA kits for human IL-1β (BD Biosciences) and IL-18 (R&D Systems).
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bioRxiv - Immunology 2022Quote: ... Cells were washed again and resuspended for 30 minutes in permeabilization buffer containing IL-17A PE (clone TC11-18H10, BD Biosciences) and primary rabbit anti-tyrosine hydroxylase antibody (clone EP1533Y ...
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bioRxiv - Immunology 2021Quote: ... primary hMDMs were transfected in a 48-well plate as described above and harvested supernatants were assayed for cytokine levels using an ELISA kit for human IL-1β (BD Biosciences) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2019Quote: IL-17A IFN-γ and IL-10 concentrations in cell culture supernatant fluids were measured by sandwich ELISA using Ab pairs from BD Pharmingen or Biolegend according to manufacturer’s suggested protocols ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated overnight at 4°C with IFN-γ APC (1:25, Clone XMG1.2, Biolegends) and IL-17 PE (1:50, Clone TC11-18H10, BD Pharmingen) in Perm/Wash buffer ...
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bioRxiv - Immunology 2020Quote: ... Interleukin-6 (IL-6) expression was measured by ELISA in SF-supernatants according to the manufacturer’s instructions (BD Biosciences, Oxford, UK).
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bioRxiv - Neuroscience 2019Quote: ... IL-6 and MIP-2 were measured using paired antibodies and recombinant cytokines according to the manufacturer’s recommendations (BD PharMingen Systems) (11).
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bioRxiv - Immunology 2019Quote: ... and IL-1β) were determined using the high-sensitivity cytometric bead array (CBA) flex system (BD Biosciences, San Jose, CA, USA) that allows multiplex quantification in a single sample ...
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bioRxiv - Immunology 2019Quote: ... Activation assays were performed by culturing cells with recombinant human IL-2 (10ng/ml, RND Systems) in the presence of coated anti-CD3 and anti-CD28 (2mg/ml, BD Biosciences) in the culture medium ...
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bioRxiv - Immunology 2020Quote: ... The cells (2-4 × 105 cells per well of a 96-well plate) were added to the ELISpot plate containing an immobilized primary antibodies to either IFN-γ or IL-4 (BD), and were exposed to various stimuli (e.g ...
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bioRxiv - Immunology 2020Quote: Supernatants were collected after 4 or 24 h and the levels of TNF and IL-1β were quantified by ELISA using OptEIA human TNF ELISA Set (BD Biosciences) and human IL-1β/IL-1F2 DuoSet ELISA (R&D systems ...
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bioRxiv - Immunology 2021Quote: ... The concentrations of IL-4 and IL-13 were measured once after collection of each individual samples by using the cytometric bead array assay (BD Biosciences) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... anti-mouse IFN-γ ELISA set (Cat no. – 555138) and anti-human IL-10 ELISA set (Cat no. – 555157) were purchased from BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... and 5e6 spleen cells were restimulated in vitro with 5e6 irradiated ERAAP KO spleen cells and 20 U/ml of recombinant human IL-2 (BD Biosciences). After 6 days ...
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bioRxiv - Immunology 2022Quote: ... Supernatants were recovered and the amounts of IL-6 were analyzed by cytokine-specific ELISA kits (BD Biosciences, Mountain View, CA).
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bioRxiv - Immunology 2023Quote: ... IL-10 and IL-13 secretion using a cytokine bead array (#I558296, #558298, #558302, #558300 and #558349 respectively and all BD Biosciences) on a flow cytometer as recommended by the manufacturer ...
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bioRxiv - Immunology 2023Quote: ... IL- 10 secretion was determined by ELISA in supernatants harvested after a 24 hour restimulation with anti-CD3 (555336, BD Biosciences) and anti-CD28 (555725 ...
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bioRxiv - Genetics 2023Quote: ... and CD43-B cells were cultured at a density of 5×105 cells per ml in RPMI medium supplemented with 15% FBS and 25 ng ml−1 of IL-4 (R&D) and anti-CD40 (BD Biosciences). Cultured cells were maintained daily at a density of 0.5-1×106 cells per ml ...
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bioRxiv - Immunology 2023Quote: ... prior to stimulation for 5 h with anti-CD3 and anti-CD28 without IL-2 in the presence of brefeldin A and monensin (BD Biosciences).
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bioRxiv - Microbiology 2023Quote: ... and values for IL-2 were determined by using a standard curve generated by using known concentrations of purified recombinant murine IL-2 (BD Biosciences).
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bioRxiv - Biochemistry 2023Quote: ... Cells were then stained for intracellular IL-6 and events were collected using the BD LSR Fortessa cell analyzer (BD Biosciences). Data was analyzed using the FlowJo Software (BD Biosciences).
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bioRxiv - Bioengineering 2024Quote: ... Cells incubated with IL-10 at 37 °C were fixed and permeabilized for 20 min on ice with Cytofix/Cytoperm (BD Biosciences). Cells were washed twice in permeabilization buffer and further stained with anti-mouse IL-10 in permeabilization buffer for 30 min at on ice ...
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bioRxiv - Genomics 2023Quote: ... Cell populations expressing the top and bottom 10% IL-1β levels were enriched using a FACSAria Fusion cell sorter (BD Biosciences). At least four million IL-1βlow and IL-1βhigh cells were collected for genomic DNA extraction using Quick-DNA FFPE Kit (Zymo Research) ...
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bioRxiv - Microbiology 2023Quote: ... We then washed the cells and stained with FACS antibodies (anti-IL-2 FITC [BD Biosciences, 562040], anti-TNF PE [BD Biosciences, 554419], anti-IFN-γ [BD Biosciences ...
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bioRxiv - Genomics 2024Quote: ... day 7 cells were transferred to fresh culture medium with IL-2 and stimulated again with peptides and 1ug/mL of anti-human CD28 antibody (BD Biosciences) for another 7 days ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells from mouse tissues were incubated with nonspecific binding blocker (anti-mouse/human CD16/CD32 Fcγ receptor; BD Biosciences) before staining for V450-human CD45 (BD Biosciences # 560368) ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells from mouse tissues were incubated with nonspecific binding blocker (anti-mouse/human CD16/CD32 Fcy receptor, BD Biosciences) before staining for APC-human CD33 (Biolegend #303408) ...
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bioRxiv - Cancer Biology 2020Quote: ... antibodies or with their isotype controls (FITC mouse IgG2a, k isotype, and APC mouse IgG2b, k isotype, BD Bioscience) in ice for 30 minutes ...
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bioRxiv - Microbiology 2021Quote: ... PE-conjugated mouse-anti-human CD8 and APC-conjugated mouse-anti-human CD3 Abs (BD Pharmingen, San Jose, CA), diluted in PBS to a final volume of 50μl/well ...
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bioRxiv - Cell Biology 2020Quote: ... 3×106 cells were incubated with purified rat IgG2b anti-mouse CD16/32 receptor antibody as per supplier’s instructions (Mouse BD Fc-block ...
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bioRxiv - Microbiology 2021Quote: ... then stained with APC-anti-mouse CD4 (Clone RM4-5) and PE-anti-mouse CD8a (Clone 53-6.72) (all from BD).
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bioRxiv - Immunology 2022Quote: Mouse thioglycollate-elicited PECs and mouse BM-Mphs were cultured in flat-bottom 96-well plates (BD Falcon, USA) at 1 × 105 cells/well ...
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bioRxiv - Immunology 2023Quote: ... Ars/A1-derived antibody secreting cells were detected with Biotin Mouse Anti-Mouse IgM[a] (Clone:DS-1; BD Biosciences), followed by Streptavidin-AP (SouthernBiotech) ...
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bioRxiv - Biophysics 2023Quote: Monoclonal mouse anti-human CD44 (Clone G44-26) and monoclonal mouse anti-CD209 (Clone DCN46) were obtained from BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... and blocked unspecific bindings with using anti-mouse CD16/CD32 (Mouse BD Fc Block, BD Biosciences, Franklin Lakes, NJ). Cells were then stained with FITC-conjugated anti-mouse CD45 (BioLegend) ...
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bioRxiv - Cell Biology 2021Quote: Serum cytokine levels (Ifn-γ, Il-1β and Tnf) were determined with a CBA Flex Set Multiplex Cytokine Bead Array (BD Biosciences).
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bioRxiv - Immunology 2022Quote: ... and the supernatants were harvested for cytokine detection (IL-10 and TGF-β) by specific ELISAs using a standard protocol (BD OptEIA). The detection limits for IL-10 is 31.3–2000 pg/ml e TGF- β is 62.5–4,000 pg/mL) ...
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bioRxiv - Immunology 2020Quote: ... IL-2 and IFN-γ concentration secreted in cell supernatant were measured using sandwich ELISA according to manufacturer’s protocol (BD Biosciences, USA). Plates were read on a Varioskan Lux multimode microplate reader (ThermoFisher ...
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bioRxiv - Immunology 2022Quote: Pro-inflammatory cytokines tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were measured in tissue culture supernatants using a commercially available sandwich ELISA kit (BD Biosciences). As recommended by the manufacturer ...
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bioRxiv - Microbiology 2019Quote: ... The cell-free supernatants were assayed for IL-1β and TNFα using a commercial Enzyme-Linked Immunosorbent Assay (ELISA) Kit (BD Bioscience-Pharmingen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Secreted concentrations of IL-13 and IFN- γ were measured from cell supernatant using a cytometric bead array (CBA, BD Biosciences, UK) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: The concentration of cytokine IL-12 present in serum samples was evaluated using the “Cytometric Bead Array” kit (CBA, BD Bioscience, USA). The analyses of these cytokines were performed using flow cytometry and the data analyzed using FCAP Array software.
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bioRxiv - Immunology 2023Quote: ... PE-cy7-conjugated anti-TNF-α (Clone MP6-XT22, 1:800), and APC-conjugated anti-IL-4 (Clone 11B11, 1:100) (BD Biosciences). All stained samples were acquired using an LSR-Fortessa flow cytometer or Symphony A3 (Becton Dickinson ...
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bioRxiv - Immunology 2023Quote: ... Cytometric Bead Array using enhanced sensitivity flex sets of human IL-2/IFN- γ/TNF- α was done following manufacturer’s instruction (BD Biosciences). Alternatively ...
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bioRxiv - Immunology 2022Quote: ... Fc receptors were blocked with anti-mouse CD16/CD32 antibodies and stained with fluorophore or biotin-conjugated anti-mouse antibodies (BD Pharmingen ...
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bioRxiv - Biophysics 2022Quote: ... mouse anti-flotillin-1 mAb (catalog number 610820) and mouse anti-calnexin mouse mAb (catalog number 610523) were purchased from BD Transduction Laboratories (Torrey Pines ...
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bioRxiv - Cancer Biology 2022Quote: ... Mouse cells were incubated with mouse BD Fc BlockTM containing purified anti-mouse CD16/CD32 mAb 2.4G2 at 1:400 (553142, BD BioSciences) for 10 min at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... Anti-mouse CD44-APC/Cy7 (clone IM7) and anti-mouse CD117-APC (c-Kit; clone 2B8) were obtained from BD Biosciences ...
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bioRxiv - Cell Biology 2020Quote: ... 3×106 cells were incubated with purified rat IgG2b anti-mouse CD16/32 receptor antibody as per supplier’s instructions (Mouse BD Fc-block, clone 2.4G2, BD Biosciences, 553141) for 20 minutes at 4°C ...