Labshake search
Citations for Becton, Dickinson and Company :
1001 - 1050 of 1102 citations for 7 Fluoro 1H indazole 3 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... anti-mouseCD86-FITC (Clone: GL1) and anti-mouse CD40-FITC (Clone: 3/23) according to the manufacturer’s instructions (BD Bioscience, USA). All controls were performed using irrelevant isotype staining ...
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bioRxiv - Immunology 2019Quote: ... Primary antibodies diluted as appropriate were: the rat anti-mouse MAC-3 antibody Clone M3/84 (1:100 dilution, BD Pharmingen), rabbit polyclonal NOS2 antibody (#ab15323 ...
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bioRxiv - Developmental Biology 2019Quote: ... This strain was grown overnight from a frozen stock (25% glycerol) in 3 mL MRS medium (DifcoTM Lactobacilli MRS Broth, BD #288110) containing 10 µg/mL chloramphenicol ...
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bioRxiv - Immunology 2020Quote: ... for 30 min prior to the addition of unconjugated anti-CD28 (clone L293) and anti-CD49d (clone L25) (each at 3 μl/ml; BD Biosciences), brefeldin A (1 μl/ml ...
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bioRxiv - Immunology 2020Quote: ... in the presence of unconjugated anti-CD28 (clone L293) and anti-CD49d (clone L25) (each at 3 μl/ml; BD Biosciences) and IL-2 (10 IU/ml ...
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bioRxiv - Neuroscience 2021Quote: ... Purified bone marrow cells were centrifuged at 1500 rpm for 3 min and filtered through a 100 μm cell strainer (BD Biosciences). The bone marrow cells were plated onto a 100 mm culture dis h or six-well plate (BD Biosciences ...
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bioRxiv - Pathology 2021Quote: ... 0.2 micron sterile filtered) using an insulin syringe (31-gauge, 6 mm length, 3/10 cc, BD Veo Ultra-Fine needle). Glucose and lactate levels were measured using a minimally invasive tail-prick method at designated timepoints post-injection.
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bioRxiv - Immunology 2021Quote: ... NK cells purity at the end of the 3-week coculture was (> 90%) as determined by flow cytometry (BD Accuri C6) using CD56 (5.11H11 ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained with cell phenotyping antibodies in a 1:1 volume ratio of 3% FBS and Brilliant Stain Buffer (BD Biosciences) according to standard procedures ...
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bioRxiv - Immunology 2022Quote: Splenocytes from infected mice were incubated for 5 hours at 37°C 5%CO2 in cRPMI prior intra-cytoplasmic detection of active-caspase 3 (BD Bioscience) using BD Fixation/Permeabilization kit (BD Bioscience) ...
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bioRxiv - Microbiology 2022Quote: ... in PBS at 37°C for 30 mins followed by one wash with PBS at 450 g for 3 mins and finally resuspend in 100 μl PBS for analysis using flow cytometry (BD Fortessa).
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bioRxiv - Microbiology 2022Quote: ... Cells were harvested and washed with 3 x with PBS before performing FACS using a BD FACS Celesta Cell Analyzer (BD Biosciences).
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bioRxiv - Physiology 2022Quote: ... Sections were washed 3 times in wash buffer then stained for Alexa Fluor 555 mouse anti-E-cadherin (BD, Cat. 560064) for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... Confluent definitive endoderm cells are then passed 1:3 in presence of Rock Inhibitor on growth factors reduced Matrigel (BD Biosciences) and cultured for 8 days in differentiation medium (DMEM F12 ...
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bioRxiv - Biochemistry 2023Quote: ... 1 µg of this genomic DNA from soluble fraction was mixed with 3 µg of anti-IdU/BrdU antibody (3D4 clone, BD Science) in BrdU-IP buffer composed of PBS and 0.0625% Triton X-100 overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... mice received a subcutaneous injection of 3 million VCaP cells in a mix of serum-free medium and 50% Matrigel (BD Biosciences). Once tumors grew to about 200 mm³ ...
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bioRxiv - Cell Biology 2024Quote: Cells were seeded 24 h before treatment and were allowed to grow in the presence of 3 μg/mL BrdU (BD Pharmingen) for 48 h ...
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bioRxiv - Immunology 2024Quote: ... and 5×104 of each were combined in 96-well U-bottom plates with anti-CD40 (100 ng/mL, clone HM40-3, BD Biosciences) in the presence or absence of OVA (10 μg/mL ...
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bioRxiv - Immunology 2023Quote: ... and ionomycin (500 ng/mL) for 3 hrs at 37°C prior to surface and intracellular staining using the BD Cytofix/Cytoperm Kit (BD Biosciences). Intracellular transcription factor staining was performed using a Foxp3 staining kit (ThermoFisher) ...
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bioRxiv - Cancer Biology 2023Quote: ... Flow cytometry was performed on a 3 laser Cytek Aurora Cytometer (Cytek Biosciences, Fremont, CA) and analyzed using FlowJo software (BD Biosciences). All antibodies are from Biolegend unless otherwise specified.
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bioRxiv - Cell Biology 2023Quote: ... After 1 h of incubation at 37 °C the cells were washed 3 times with HBSS followed by trypsinization and signal was measured using FACScan and FACSCalibur systems (BD Biosciences). Data was analyzed with FlowJo™ v10 Software (BD Biosciences).
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bioRxiv - Cancer Biology 2023Quote: ... Flow cytometry was performed on a 3 laser Cytek Aurora Cytometer (Cytek Biosciences, Fremont, CA) and analyzed using FlowJo software (BD Biosciences). All antibodies are from Biolegend ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibody incubation was overnight at 4 degrees C with the following antibodies at 1:100 dilution in PBS + 3% BSA: CD24-FITC (clone ML5, BD Bioscience), CD271-APC (clone C40-1457 ...
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bioRxiv - Immunology 2023Quote: ... Pooled cells from n = 3 - 5 mice/cohort were stained with PE- or APC-conjugated antibodies (from either BD or BioLegend) against murine F4/80 (clone ...
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bioRxiv - Immunology 2023Quote: ... with 3×104 PFU or injected intracranially (10uL) with 50 PFU of LGTV-TP21 using insulin syringes (BD Medical, #BD-329461). At appropriate times post infection ...
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bioRxiv - Biophysics 2022Quote: ... #131-8; #130-6; #130-5; #130-3 and 2 μm lot MM2307#156; #159.1; #159.2; #122.3, BD Biosciences, San Jose, CA). For calibration of the fluorescence axis in the PE channel ...
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bioRxiv - Immunology 2023Quote: ... and 1:20 dilution mouse anti-human CD123 (IL-3)-PE (stock 200 ug/mL, BD Biosciences, Franklin Lakes, NJ, USA). A 1:1,000 dilution of anti-mouse IgG Alexa-647 (stock 1 mg/mL ...
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bioRxiv - Microbiology 2023Quote: ... cells were incubated with fluorochrome-conjugated monoclonal antibodies (mAbs) diluted at 1:200 into a 1:3 solution of Brilliant Staining Buffer (BD 563794): Cell Staining Buffer ...
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bioRxiv - Cell Biology 2023Quote: ... E8.5 embryos were fixed with 4% paraformaldehyde (PFA) in PBS at 4°C for 3 h and incubated with FITC-conjugated anti-EEA1 (1:100, BD Bioscience) or Cy3-labeled anti-mouse IgG (1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... The cells followed by an incubation with the SM/Chol probe OlyA (3 µM) and primary anti-IFN-γR1 antibody (1:50) (BD Biosciences) together in 1% BSA-PBS at room temperature for 90 min ...
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IpaA reveals distinct modes of vinculin activation during Shigella invasion and cell-matrix adhesionbioRxiv - Cell Biology 2023Quote: ... NJ) were coated for 3 hours with 10 μg of Matrigel following the manufacturer’s instructions (Biocoat, BD Biosciences, San Jose, CA). Inserts were placed into 24-well dishes containing 500 μl of RPMI medium supplemented with 1% fetal calf serum ...
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bioRxiv - Evolutionary Biology 2023Quote: ... After 48h cultures were diluted and plated on Nutrient Agar plates (5 g/L peptone BD difco, BD Bioscience; 3 g/L yeast extract BD difco, BD Bioscience, 15 g/L agar Bacto, BD Bioscience). Plates were left at room temperature until colonies were detectable and distinguishable (2-4 days) ...
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bioRxiv - Plant Biology 2024Quote: ... The dry volume (DV, m3) was measured by water displacement (Berta et al., 2010) and basic density (BD, kg m-3) calculated as reported by Carriero et al ...
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bioRxiv - Systems Biology 2024Quote: ... freshly sorted lineage-labeled Dclk1+ cells were resuspended in LPM-3D medium (LPM-3) and mixed with growth factor-reduced Matrigel (BD Biosciences) in a 1:1 ratio ...
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bioRxiv - Microbiology 2024Quote: ... Cells were activated for 3 days with 1 μg/mL/million cells of purified NA/LE mouse anti-human CD3 and CD28 (BD Biosciences) and infected with HIVBaL at 0.01 ng/106 cells ...
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bioRxiv - Immunology 2024Quote: ... coated plates were blocked with PBS–3% rat serum and incubated with serum samples and with a biotinylated rat antimouse IgE (clone R35–118; BD Biosciences). Avidin-HRP (Ebiosciences ...
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bioRxiv - Immunology 2024Quote: ... 50 μl of PBS-BSA containing 2.5 μl of CD11c APC antibody (clone S-HCL-3) or 5 μl of CD11c BV421 antibody (clone B-ly6 RUO) (BD Biosciences) were added to each tube ...
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bioRxiv - Immunology 2024Quote: ... Flow cytometry was performed on a 3 laser Cytek Aurora Cytometer (Cytek Biosciences, Fremont, CA) and analyzed using FlowJo software (BD Biosciences). All antibodies are from Biolegend ...
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bioRxiv - Bioengineering 2024Quote: ... 25,000 irradiated HM-1-luc cells were mixed with 3 × 105 PBMCs per sample and seeded in a 96-well ELISPOT plate (BD Biosciences) that was pre-coated with IFN-γ capture antibody (BD Biosciences) ...
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Planarian CREB-binding protein (CBP) gene family regulates stem cell maintenance and differentiationbioRxiv - Developmental Biology 2020Quote: ... which was incubated for 2 h in the dark at 37°C with 20 µM caspase-3 substrate Ac-DEVD-AMC (BD Biosciences Pharmingen) or 2 ml from a stock of 1 mg/ml for a final volume of 150 µl ...
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bioRxiv - Developmental Biology 2022Quote: Zebrafish wild-type and vsxKO retina sections from different developmental stages were analysed for the detection of apoptotic and mitotic cells using rabbit anti-active caspase-3 antibodies (BD Biosciences, 559565) and rabbit anti-phospho-histone H3 antibodies (Merck Millipore ...
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bioRxiv - Immunology 2021Quote: ... cells were stained for surface markers (when required) then fixed/permeabilized by using PE Active Caspase-3 Apoptosis Kit (BD Bioscience, 550914) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... The cell pellet was then resuspended in 1 ml of T-R minimal medium and injected into the IsoCaRB system using a 3 ml syringe (BD Biosciences # 309657) and a 20-gauge needle (Sigma-Aldrich #Z192511).
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bioRxiv - Molecular Biology 2021Quote: ... Between 2 - 4x105 events were collected per sample within 3 hours after staining on an LSRFortessa (BD Biosciences, 4 lasers, 18 parameters) and analyzed using FlowJo software (version 9.7.4 ...
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bioRxiv - Cancer Biology 2020Quote: ... Mammary fat pad injections were performed in the 4th pre-cleared mammary fat pad of 3 weeks old female mice in 50% matrigel (BD Biosciences, Germany). Breast or prostate cancer-origin of xenografts were verified by a pathologist.
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bioRxiv - Cancer Biology 2019Quote: ... Parental P18T and RAS pathway mutant knock-in patient-derived organoids were transplanted subcutaneous as single cells at a density of 3×10^5 organoids in 100 µl 50% Matrigel/medium with 10% collagen type I (BD Bio-sciences) mixture into NSG-B2m mice (JAX stock no ...
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ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactionsbioRxiv - Microbiology 2021Quote: ... Lentivirus was titrated by serial dilution of lentivirus and incubating with 5000 HEK293T cells in 96 well plates with 8 µg/ml polybrene for 3 days followed by GFP detection by flow cytometry (BD Biosciences LSRFortessa). Transduction units (TU ...
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bioRxiv - Immunology 2022Quote: ... 1x105 tumor cells and 1x105 γδ T cells were co-cultured for 3 h at 37°C in the presence of 1 µl α-CD107a-PE antibody (BD Biosciences). Medium or stimulation with α-CD3 and α-CD28 (both 1 µg/ml ...
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bioRxiv - Immunology 2022Quote: ... PBMC from the 3-time point from each patient were labelled using Single Cell Labelling with the BD Single-Cell Multiplexing Kit (BD Biosciences, #633781) and 52 different BD AbSeq Ab-Oligos reagents following the manufacturer’s protocol (BD Biosciences) ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were suspended in 500 μl PBS with 3 % FBS and then detected by flow cytometry (BD Biosciences, San Diego, CA, USA).