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Citations for Becton, Dickinson and Company :
51 - 100 of 1274 citations for SARS CoV 2 Spike Glycoprotein S1 RBD His Tag CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... the ELISA was performed by coating 96 well plates with 2 µg/mL LRRC15-His (AcroBio, #LR5-H52H3) in coating buffer (BD, #51-2713KC) overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... CD69 BV711 (Clone HI.2F3) (BD Cat. 740664), CD103 PE-CF594 (Clone M290 ...
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bioRxiv - Immunology 2020Quote: Cells were stimulated for 12h with SARS-CoV-2 peptide pools (0.25 μg/ml) in the presence of αCD28/αCD49d co-stimulatory antibodies (BD FastImmune™, BD Biosciences, San Diego, CA, USA), 5 μg/ml brefeldin A (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... and Sample Tag barcodes were captured by BD Rhapsody beads coated with poly(T ...
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bioRxiv - Neuroscience 2023Quote: ... and Sample Tag libraries (BD Biosciences, cat. 633801) for sequencing on Illumina® sequencers ...
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bioRxiv - Molecular Biology 2023Quote: Genes encoding HBb (BD_RS00255, old locus tag: Bd0055) and HMfB (GenBank accession number M34778.1 ...
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bioRxiv - Bioengineering 2023Quote: ... the GFP+IgG+ cell population (B-RBD cells) were sorted by FACS (BD Aria III).
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bioRxiv - Cancer Biology 2024Quote: SV40 TAG-specific mouse monoclonal antibody (BD Bioscience 554149) primary antibody was used ...
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bioRxiv - Immunology 2019Quote: ... and Foxp3 (S1 Table) and analyzed using a Fortessa flow cytometer running FACSDiva (BD Biosciences). FSC 3.0 files were analyzed and presented with FLOWJO Software (flowjo.com).
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bioRxiv - Microbiology 2023Quote: ... we stained cells expressing wild-type and mutant SFV glycoproteins with a mouse anti-SFV immune ascitic fluid (ATCC Cat#: VR-1247AF) or a control mouse IgG2b antibody (BD Biosciences Cat#: 557351). Staining of wild-type and mutant EEEV or SFV E3–E2–(6K/TF)–E1 was detected with a PE-conjugated donkey anti-mouse F(ab’)2 fragment (Jackson ImmunoResearch Cat# ...
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bioRxiv - Immunology 2023Quote: ... Alive DAPI- CD19+RBD+ B cells were single-cell index sorted using a FACSAria II (BD Biosciences) into empty 96-well PCR plates (VWR) ...
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bioRxiv - Immunology 2023Quote: RBD-specific T cell responses were investigated using the mouse IFN-γ ELISPOT Set (551083; BD Biosciences), as per manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: CHO cells stably expressing the ecDHFR mutant library were submitted for flow cytometry twice (FACSAria, (BD Biosciences, Franklin Lakes, NJ), UT Southwestern Mass and Flow Cytometry Core ...
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bioRxiv - Bioengineering 2021Quote: ... CHO-S wild type cells at a concentration of 1 × 106 cells/mL in a six well plate (BD Biosciences) in AC free media were transfected with a total of 3.75 μg DNA using FreeStyleTM MAX reagent together with OptiPRO SFM medium (Life Technologies ...
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bioRxiv - Bioengineering 2023Quote: CHO MPs were counted using either a BD FACSAriaII flow cytometer with FACSDiva software (BD Biosciences, Franklin Lakes, NJ, USA) or a CytoFLEX S flow cytometer with CytExpert software (Beckman Coulter ...
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bioRxiv - Genetics 2021Quote: ... Cytofluorimetric analysis was performed to enumerate CD20 positive and mCherry positive CHO-k cells using the BD FACSCalibur sytem (BD Biosciences).
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bioRxiv - Immunology 2023Quote: ... anti-CD8 (as above) and anti-CD69 (HI.2F3, BD Bioscience) or anti-IRF4 (3e4 ...
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The skin environment controls local dendritic cell differentiation and function through innate IL-13bioRxiv - Immunology 2021Quote: ... lung and SI dLNs as indicated in Figure S1 using a BD INFLUX Cell sorter (BD Biosciences). Cells were resuspended in FACS buffer and the respective DC2 subets were sorted directly into QIAzol Lysis Reagent (QIAGEN ...
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bioRxiv - Immunology 2023Quote: ... and CD45 Sample Tags to enable multiplexing (BD Bioscience, RRID: AB_2870301) and AbSeq antibodies to ICOS (AMM2072 ...
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bioRxiv - Immunology 2021Quote: ... BCR triggering was measured in response to the RBD-NPs described above by flow cytometry (LSR II, BD Biosciences) as the ratio of Ca2+ bound/unbound states of Fura red ...
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bioRxiv - Immunology 2022Quote: ... and biotinylated RBD Gamma and Delta with streptavidin-BV480 or streptavidin-BV650 (all from BD Biosciences, San Jose, CA) at a protein ...
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bioRxiv - Microbiology 2024Quote: ... Cell samples were acquired on BD LSR Fortessa and data were analyzed (Figure S1) using FlowJo 10 (BD).
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bioRxiv - Immunology 2021Quote: ... Plates were washed five times and developed with tetramethylbenzidine (1-Step Ultra TMB-ELISA Substrate Solution, ThermoFisher, for RBD-ELISA, and BD OptEIA Substrate Solution ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed five times and developed with tetramethylbenzidine (1-Step Ultra TMB-ELISA Substrate Solution, ThermoFisher, for RBD-ELISA,and BD OptEIA Substrate Solution ...
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bioRxiv - Immunology 2024Quote: ... and stained for surface and intracellular markers using the antibodies listed in Table S1 for flow cytometry analysis (BD FACS Canto II ...
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bioRxiv - Cell Biology 2021Quote: ... bound proteins were analyzed by western blotting using anti-His monoclonal antibodies (BD Biosciences ...
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bioRxiv - Microbiology 2021Quote: ... GFP-positive transduced CHOK1 cells expressing RBD-mFc were sorted by flow cytometry using a BD FACSAria III cell sorter (BD Biosciences) and suspension adapted in PROCHO5 medium (Lonza ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated antibody bound to RBD in the presence of competitor was stained with Streptavidin-PE at a 1:1000 dilution (BD Biosciences) and analysed on a 200 Bioplex instruments ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed five times and developed with tetramethylbenzidine (1-Step Ultra TMB-ELISA Substrate Solution, Thermo Fisher Scientific, for the RBD ELISA, and BD OptEIA Substrate Solution ...
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bioRxiv - Immunology 2021Quote: ... Eosinophils were labelled with sample tags (633793 BD Mouse Single-Cell Multiplexing Kit) according to the the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... cells were labelled with sample tags (633793 BD Mouse Single-Cell Multiplexing Kit) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... Single-cells were purified from the population of Spike-binding cells by flow cytometry using a FACSMelody (BD). Single-cell clones were then assessed for binding to His-tagged SARS-CoV-2 Spike using a Cytoflex S (Beckman ...
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bioRxiv - Immunology 2021Quote: ... The binding of mAbs to spikes expressing on cell surface was analyzed using flow cytometry (BD Canto Analyzer). Percent (% ...
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bioRxiv - Biophysics 2022Quote: ... The binding of mAbs to spikes expressing on cell surface was analyzed using flow cytometry (BD Canto Analyzer). Percent (% ...
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bioRxiv - Molecular Biology 2020Quote: ... Four-way cell sorting (spermatogonia, primary spermatocytes, secondary spermatocytes, round spermatids; File S1) using a FACSAria II Cell Sorter (BD Biosciences ...
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bioRxiv - Biochemistry 2023Quote: ... 150,000 cells were sorted in early (S1) and late (S2) S-phase fractions using a Fluorescence Activated Cell Sorting system (FACS Aria Fusion, BD) in Lysis Buffer (50 mM Tris pH 8 ...
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bioRxiv - Immunology 2024Quote: ... and stained for surface and intracellular markers using the antibodies listed in Table S1 for flow cytometry analysis (BD FACS Canto II, BD FACSARIA III ...
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bioRxiv - Bioengineering 2019Quote: ... the cell culture mixture was stained with an antibody recognizing the neural cell adhesion molecule (NCAM/CD56; BD Pharmingen; Table S1), and the myogenic progenitor (CD56+ ...
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bioRxiv - Immunology 2021Quote: ... Cells were aliquoted to a count of 1 million cells each and incubated on ice with Fc Block (BD, Table S1) at a 1:20 dilution ...
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bioRxiv - Immunology 2019Quote: ... Cells were initially stained with fluorochrome-conjugated antibodies against surface receptors (see Table S1) in BD Brilliant stain buffer (BD Biosciences) for 30 min at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: Hematopoietic cells were stained with the antibodies listed in Supp Table S1 for 20-30 min at 4°C and analyzed on a custom BD LSR Fortessa (BD Biosciences). For sorting ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were stimulated with the RBD peptide pool (5 μg.ml−1 final concentration) and brefeldin A (1 μg.ml−1 ; BD Pharmingen, San Diego, CA, USA) at 37 °C overnight ...
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bioRxiv - Immunology 2021Quote: ... FNA samples were stained in P2 for 30 minutes on ice with biotinylated and Alexa Fluor 647 conjugated recombinant soluble Spike proteins as well as PD-1 BB515 (EH12.1, BD Horizon). Cells were then washed twice with P2 and stained with IgG BV480 (goat polyclonal ...
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bioRxiv - Epidemiology 2019Quote: ... 2011 and 2015 were available for four of these 12 counties (Dalarna-AR, Örebro-AR, Uppsala-BD and Norrbotten-BD, Table S1).7 We had data about the yearly number of CT diagnoses from all counties since 1997.11 In the model ...
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bioRxiv - Epidemiology 2019Quote: ... 2011 and 2015 were available for four of these 12 counties (Dalarna-AR, Örebro-AR, Uppsala-BD and Norrbotten-BD, Table S1).7 We had data about the yearly number of CT diagnoses from all counties since 1997.11 In the model ...
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bioRxiv - Immunology 2024Quote: ... Single-cell suspensions were stained with antibodies listed in Table S1 and acquired with a LSR Fortessa flow cytometer (BD Bioscience, US). For cell-associated autofluorescence to be removed in the indicated experiments ...
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bioRxiv - Immunology 2023Quote: ... Ancestral and variant spike expressing HEK293T cells were sorted for as target cells using the FACSAria™ Fusion Cell Sorter (BD Biosciences ...
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bioRxiv - Microbiology 2023Quote: ... Yersinia pestis strains were routinely grown in Heart Infusion (HI) broth (Difco, BD, Franklin Lakes, NJ) or on HI agar at 28°C ...
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bioRxiv - Immunology 2023Quote: ... Surface antibodies were prepared at optimal dilutions (see Supplementary Table S1) in FACS supplemented with Brilliant Stain Plus buffer (BD; Franklin Lakes, NJ), then were added to samples in blocking solution ...
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bioRxiv - Immunology 2022Quote: ... an Alexa Fluor 647 AffiniPure anti-HA tag Goat Anti-Mouse IgG was used (BD Bioscience). A375 stained with anti-AXL PE-conjugated antibodies (FAB154P ...