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Citations for Becton, Dickinson and Company :
9701 - 9750 of 10000+ citations for Mouse Anti Respiratory Syncytial Virus Antibody RV3 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... The permeabilized cells were stained with intracellular cytokine staining antibodies for 20 min at room temperature in 1 perm/wash buffer (BD Biosciences). Cells were then washed twice with perm/wash buffer and once with staining buffer before acquisition using the BD Symphony Flow Cytometer and the associated BD FACS Diva software ...
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bioRxiv - Cell Biology 2022Quote: ... cell sorting was employed by labeling intact cells with the fluorescently conjugated HER2 antibody ErbB2 PE-Vio770 and then subjected to fluorescence-activated cell sorting using a FACS Aria III (BD Biosciences), selecting the cells in the top 10% of HER2 expression to generate MDA-MB-231-HER2 stable cells.
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Cardiac fibroblasts regulate cardiomyocyte hypertrophy through dynamic regulation of type I collagenbioRxiv - Cell Biology 2022Quote: ... and the cells were labeled with antibodies as also described above and were then analyzed using a BD FACSCanto cytometer running BD FACSDiVa software (BD Biosciences), using a violet (405 nm ...
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bioRxiv - Genomics 2022Quote: ... This was followed by the secondary labeling step with 1:200 dilution of Streptavidin PE-Cy7 antibody (BD Biosciences, Cat#: 557598). Cells were isolated and sorted using an Influx Cell Sorter Instrument (BD Biosciences ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed twice with 500 μL permeabilization buffer (from kit described above) and stained with 150 μL permeabilization buffer containing 1:400 Ki67-Alexa-Fluor antibody (561126, BD Biosciences) for 30 minutes at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... The cells were stained with fluorochrome-conjugated antibodies or influenza HA-specific (HA518–526) H-2Kd tetramer-IYSTVASSL (MBL) reagent and analyzed using a flow cytometer (BD Biosciences). BMDCs (105 cells ...
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bioRxiv - Neuroscience 2019Quote: ... IL-6 and MIP-2 were measured using paired antibodies and recombinant cytokines according to the manufacturer’s recommendations (BD PharMingen Systems) (11).
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bioRxiv - Cancer Biology 2019Quote: DMS-53 cells transduced with DARPP-32 shRNAs or exogenously overexpressed DARPP-32 isoforms were stained with Annexin V-FITC antibodies (BD Biosciences) to assess apoptosis ...
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bioRxiv - Biochemistry 2019Quote: ... After washing with flow cytometry buffer cells were incubated with secondary antibodies for 30 min on ice and wash twice and analyzed by FACScanto instrument (BD Biosciences). Cell fixation and permeabilization was carried out using the Fixation/Permeabilization Solution Kit (BD Biosciences) ...
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bioRxiv - Cell Biology 2019Quote: ... HMLER CD44low/high cells stained with CD24-APC and CD44-PE antibodies were sorted by FACS using an Aria IIu (BD Biosciences) to obtain isolated CD44high/CD24low and CD44low/CD44high cell populations ...
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bioRxiv - Immunology 2020Quote: Colonic LP samples (2 million cell/staining) were stained at 4°C for 20 min with the following antibodies: Ly6C-FITC (AL-21, BD Pharmingen), F4/80-PE (BM8 ...
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bioRxiv - Developmental Biology 2021Quote: ... Primary antibodies were incubated overnight at 4°C and secondary antibodies for 30 min in RT in Perm/Wash buffer (554714, BD Biosciences) containing 5% FBS ...
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bioRxiv - Immunology 2019Quote: ... after which cells were stained with Fixable Viability Staining e780 (1:1000) and CD11B (M1/70) antibodies: CD11B-BV510 (Day 1 cells, 1:300, BD Biosciences), CD11B-Percp-Cy5.5 (Day 3 cells ...
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bioRxiv - Developmental Biology 2021Quote: ... which were then stained with the above-mentioned antibodies and LT-HSCs were sorted using a BD FACS Aria III (BD Biosciences).
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bioRxiv - Immunology 2020Quote: PBMCs or purified T cells were stained for 30 minutes on ice with the antibodies specific for extracellular proteins in Brilliant Stain Buffer (BD, 563794). Following incubation ...
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bioRxiv - Immunology 2020Quote: We centrifuged the samples and resuspended each in 1 mL of an antibody master mix consisting of Brilliant Buffer (BD Biosciences) and anti-CCR6 - PECy7 (BioLegend ...
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bioRxiv - Immunology 2019Quote: Enriched populations of cells obtained after magnetic selection protocols were incubated with a cocktail of antibodies and sorted by FACS: CD3ε-FITC (clone UCHT1, BD Biosciences), CD4-APC Cy7 (clone RPA-T4 ...
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bioRxiv - Immunology 2019Quote: ... washed twice in FACS wash and incubated with the following antibodies for 1h at room temperature: CD4 FITC (clone SK3, BD Biosciences), CD3 Alexa 700 (clone UCHT1 ...
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bioRxiv - Immunology 2019Quote: ... were resuspended in D-PBS supplemented with 2% (v/v) FBS containing the antibody cocktail in 96-well microtiter plates (BD Biosciences) for 20 min on ice in the dark ...
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bioRxiv - Molecular Biology 2019Quote: ... 20μg of total protein was loaded per lane and probed with the following primary antibodies dissolved in TBS-T: Topoisomerase IIβ (1:1000, overnight) (BD Biosciences), αTubulin (DMIA ...
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bioRxiv - Cell Biology 2020Quote: ... the cell surface of freshly-prepared epidermal cells was labeled with the following antihuman protein antibodies (clone, manufacturer): FITC–conjugated CD1a (HI149) and PE–conjugated HLA-DR (G46-6, both BD Pharmingen), PE–CF594–conjugated CD3 (UCHT1 ...
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bioRxiv - Cell Biology 2020Quote: ... The purity of the cells was confirmed by staining cells with PE-CD71 and allophycocyanin (APC)-Ter119 antibodies (all from BD Biosciences) by flow cytometry ...
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bioRxiv - Cell Biology 2020Quote: ... Kidney sections were blocked (10% normal goat serum and 1% bovine serum albumin (BSA) in PBS) and incubated overnight at 4°C with primary antibody ((Ly6G, 1:10; BD/Pharmingen), FITC-C3 (1:200 ...
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bioRxiv - Immunology 2021Quote: ... The cells were washed with the medium and stained with subsequent antibodies/reagents using the Brilliant Stain Buffer Plus (BD Biosciences) for 30 min at room temperature ...
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bioRxiv - Genomics 2020Quote: ... Cells were then incubated with 5ul human Fc Block with 95ul 2% FBS in PBS at 4°C for 15 mins before staining with SCMK and AbSeq antibodies (BD Biosciences) at 4°C for 60 min ...
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bioRxiv - Immunology 2021Quote: ... The stained cells were subsequently washed with FACS buffer to remove unbound antibodies and probe and then filtered through 70-μm cell mesh (BD Biosciences) before processed in the FACS sorter ...
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bioRxiv - Immunology 2021Quote: ... cryo-preserved PBMCs were first thawed at 37°C in a water bath and washed with an RPMI medium containing 10% fetal bovine sera (FBS) following incubation with fluorescently-labeled antibodies (BD Biosciences) against cell surface markers for CD3 (PE-Cy7) ...
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bioRxiv - Developmental Biology 2021Quote: Mouse femur single cells were obtained as described above in the flow cytometry section and stained with biotin-conjugated antibodies for Lin (Ter119, Gr-1, B220, CD3e) (BD, 559971) followed by negative selection using streptavidin-coated magnet beads (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2019Quote: ... Primary hMPCs were sorted using fluorescence activated cell sorting with fluorescently-conjugated antibodies to cell surface antigens specific to hMPCs [CD56 (NCAM, BD Pharmingen) and CD29 (β1-integrin ...
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bioRxiv - Cancer Biology 2021Quote: ... each sample was stained with the corresponding primary antibody-dye conjugates diluted in 100 μl Pharmingen stain buffer (BD Biosciences, #554657). After 1 h staining at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... and 2 mM KH2PO4)-FBS 5% for 15 min and then stained for surface expression with fluorophore-marked antibodies against SLAMF1 (BD, 559572), CD31 (BD ...
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bioRxiv - Immunology 2020Quote: ... The cells (2-4 × 105 cells per well of a 96-well plate) were added to the ELISpot plate containing an immobilized primary antibodies to either IFN-γ or IL-4 (BD), and were exposed to various stimuli (e.g ...
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bioRxiv - Immunology 2021Quote: ... 4 and 6 hours and cytokines detected by intracellular cytokine staining (see Table S2 for antibodies list) using Fixation/Permeabilization Solution Kit (BD Biosciences), unstimulated cells were used as a control.
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bioRxiv - Neuroscience 2020Quote: ... The cells were stained with fluorophore-conjugated antibodies and analyzed on a MACSQuant flow cytometer (Miltenyi Biotech) or sorted using the FACSAria Fusion cell sorter (BD Biosciences). Flow cytometry analysis was based on viable and single-cell gaiting strategies ...
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bioRxiv - Immunology 2020Quote: ... Single-cell suspensions were first stained for viability with LiveDead eFlour 780 stain (eBioscience) and stained with antibodies diluted in Brilliant Stain Buffer (BD Biosciences) or FACS buffer (1% BSA in PBS) ...
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bioRxiv - Immunology 2020Quote: ... for the last 4hrs before subjecting for fluorochrome-conjugated antibody staining for surface markers and intracellular cytokine (Perm/Fix buffer, BD Biosciences). Cells were analyzed by 24-color compatible full-spectrum Cytek Aurora flow analyzer (College of Veterinary Medicine ...
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bioRxiv - Immunology 2020Quote: ... cells were first stained for viability with LiveDead eFlour 780 stain (eBioscience) for 30 minutes and then stained with antibodies and MHC I tetramers diluted in Brilliant Stain Buffer (BD Biosciences) for 60 minutes ...
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bioRxiv - Immunology 2020Quote: ... and BM as previously described (Ciucci et al., 2015) and were stained with different antibodies used for FACS (FACS canto, BD Biosciences) are indicated in the figures.
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bioRxiv - Immunology 2020Quote: Single-cell suspensions from the intestine or bone marrow were stained with fluorescently labelled antibodies and sorted (purity > 98%) using a BD FACSAria III cell sorter (BD Biosciences). For ILC ...
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bioRxiv - Immunology 2020Quote: ... Purified MDSCs from spleen and bone marrow cells were isolated using a mojosort magnet followed by cell surface staining with CD11b and Gr1 monoclonal antibodies for cell sorting on BD FACS Aria III cell sorter (BD Biosciences). Similarly ...
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bioRxiv - Immunology 2020Quote: ... CD8+ T cells were obtained from the spleen of naive mice following antibody labeling and sorted on flow cytometer BD FACS Aria III cell sorter (BD Biosciences).
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bioRxiv - Immunology 2020Quote: ... IFNγ-producing cells were stained with IFNγ Detection Antibody in conjunction with cell surface markers (listed below) and analyzed on an LSRFortessa flow cytometer (BD Biosciences). Antibodies used included CD3e (145-2C11) ...
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bioRxiv - Immunology 2021Quote: ... as a negative control in the presence of αCD28/αCD49d co-Stim antibodies (1 μg ml−1) GolgiStop (containing Monensin, 2 μmol/L), GolgiPlug (containing brefeldin A, 10 μg ml−1) (BD Biosciences) and anti-CD107α BV421 antibody (BD Biosciences) ...
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bioRxiv - Immunology 2021Quote: ... The permeabilized cells were stained with ICS antibodies for 20 min at room temperature in 1X-perm/wash buffer (BD Biosciences). Cells were then washed twice with perm/wash buffer and once with staining buffer before analysis using BD Symphony Flow Cytometer ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained with cell phenotyping antibodies in a 1:1 volume ratio of 3% FBS and Brilliant Stain Buffer (BD Biosciences) according to standard procedures ...
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bioRxiv - Immunology 2021Quote: ... The permeabilized cells were stained with ICS antibody cocktail (Suppl. Table 2) for 20 min at room temperature in 1X-perm/wash buffer (BD Biosciences). Cells were then washed twice with perm/wash buffer (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... Up to 5×106 cells were washed in cold FACS wash (PBS supplemented with 2% FCS and 2mM EDTA) prior to being stained with a surface receptor antibody cocktail containing FcBlock (BD, 2.4G2) and LIVE/DEAD fixable blue dead cell stain (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained with a cocktail of antibodies (P1, P2, P3 and P4) in Cell Staining Buffer plus BD Horizon Brilliant Stain Buffer Plus (BD, 566385) (Key Resources Table ...
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bioRxiv - Immunology 2022Quote: ... cells were fixed with fixation buffer and then permeabilized with Cytofix / Cytoperm (e-bioscience) followed by staining for 60 minutes with antibodies that were diluted in 1X perm buffer (BD, 554723). Flow cytometry data acquisition was performed either on LSR Fortessa X-20 (BD ...
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bioRxiv - Cancer Biology 2022Quote: The fraction of CD44+/CD24− cells was determined by trypsinizing and resuspending cells in FACS buffer at 10×106 cells/mL followed by incubation with antibodies against human CD44 (APC-conjugated, Clone G44-26, 1:5, BD Biosciences) and CD24 (PE-Cy7-conjugated ...