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Citations for Becton, Dickinson and Company :
901 - 950 of 2340 citations for Recombinant Human TNFRSF4 Protein Fc Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... naïve splenic B cells were enriched by the depletion of CD43 expressing cell using biotinylated anti-CD43 antibody (Clone S7, BD Biosciences), BD IMag Streptavidin Particles Plus and the IMag® system (BD Biosciences) ...
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bioRxiv - Immunology 2020Quote: ... Plates were then washed and incubated with detection biotinylated anti-IFNγ antibody for 2h and followed by incubation with streptavidin-horse radish peroxidase (BD Biosciences) for 1h ...
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bioRxiv - Immunology 2020Quote: ... The cells were fixed with 4% neutrally buffered formalin for 5 min and stained with anti-mouse primary antibodies (1:100, anti-mouse LC3B, LAMP-1 and p62 antibodies) followed by a biotinylated anti-Ig secondary antibody (BD Biosciences) and streptavidin-PE ...
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bioRxiv - Immunology 2022Quote: ... ELISAs were performed using paired rat anti-mouse IgE antibodies (capture with 1 μg/mL purified R35-72, BD Biosciences, detect with biotinylated R35-118, BD Biosciences) and streptavidin-HRP (BD Biosciences) ...
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bioRxiv - Immunology 2023Quote: ... plates were incubated with serum (2 fold dilutions, 1:20-1:2560) and parasite specific antibody was measured using biotinylated IgG1 (BD Biosciences) or IgG2c (BD Biosciences ...
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bioRxiv - Cell Biology 2022Quote: ... for epitope retrieval and incubation with primary anti-Collagen Type II (DSHB II-II6B3) followed by a biotinylated secondary and Streptavidin-HRP (BD Biosciences). II-II6B3 was deposited to the DSHB by Linsenmayer ...
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bioRxiv - Cancer Biology 2023Quote: ... mice bone marrow mononuclear cells were first stained with lineage (Lin) cocktail containing biotinylated antibodies against Ly-6G/Ly-6C (Gr-1) Biotin RB6-8C5 (BD Bioscience) (1:100) ...
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bioRxiv - Immunology 2023Quote: ... the plate was washed 5x with PBST 0.05% and incubated with biotinylated antibody G1 (Serotec MCA336B) or G2A (BD bioscience 553388) for 60min at RT ...
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bioRxiv - Immunology 2024Quote: ... wells were incubated with 100 µL of biotinylated anti-IgG1 (1 µg/mL in PBS with 2% FBS, clone A85-1, BD Biosciences) for 2 hours at RT ...
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bioRxiv - Immunology 2021Quote: ... PBMCs and mdDCs culture supernatants were quantified by the Cytometric Bead Array (CBA) method using the Human Inflammatory Cytokines Kit and Human Chemokine Kit (BD Biosciences), following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated with PE-conjugated mouse anti-Human CD44 and mouse anti-Human CD184 antibodies (BD Biosciences, San Jose, CA) at a concentration of 5µl/10 million cells ...
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bioRxiv - Immunology 2020Quote: ... Flow cytometry experiments were performed using PE-anti-human CD54 (ICAM-1) (REA266, Miltenyi) and APC-anti-human CD20 (clone L27, BD Biosciences). Antibodies for blocking LFA-1 - ICAM-1 interaction were anti-ICAM1 (clone HA58 ...
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bioRxiv - Microbiology 2021Quote: ... Purification of human CD34+ cells were assessed by flow cytometry using an anti-human CD34-FITC antibody (clone 581, BD Biosciences). Fetal liver derived from three different donors were used in this study.
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bioRxiv - Immunology 2022Quote: Suspensions of human Tconv cells or Treg cells were stained with V500-conjugated anti-human CD4 (clone RPA-T4, BD Biosciences), PE-Cy7-conjugated anti-human CD25 (clone BC96 ...
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bioRxiv - Genetics 2020Quote: ... and permeabilized using BD Cytofix/Cytoperm kit before incubation with BV605 anti-human IFN-gamma (B27 clone) and BUV395 anti-human TNF-alfa (MAB11 clone, BD Biosciences) in permeabilization buffer at 4°C for 30 min in the dark ...
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bioRxiv - Immunology 2020Quote: ... mouse anti human ICAM-1 (CD54) and mouse anti human VCAM-1 (CD106) antibodies and fixed with FACS lysing solution (BD Bioscience). Surface levels were determined by flow cytometry (Gallios ...
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated with PE-conjugated mouse anti-Human CD44 and mouse anti-Human CD184 antibodies (BD Biosciences, San Jose, CA) at a concentration of 5μL/10 million cells ...
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bioRxiv - Cancer Biology 2021Quote: ... cells from digested tumors were incubated with Fc block (5 µg/mL; BD Biosciences, CA, USA; clone 2.4G2) and stained with antibody mix for 30 min and Viability Dye eFluor™ 780 (eBioscience ...
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bioRxiv - Pathology 2022Quote: ... Murine Fc receptors were blocked using anti-mouse CD16/CD32 (FcBlock clone 2.4G2, BD Biosciences, #553141, 1/200) and cells were stained in FACS buffer for 30 min at 4°C with the following anti-mouse antibodies ...
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bioRxiv - Immunology 2022Quote: ... cell suspensions were first incubated with mouse CD16/CD32 Fc–blocking antibodies (clone 2.4G2, BD Biosciences Cat# 553142) and Aqua LIVE/DEAD® Fixable Dead Cell Stain (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... in FACS buffer (PBS, 1% FCS, 0.02% Sodium Azide, 2mM EDTA) and 50μl of BD Brilliant stain buffer (BD) for 30 minutes on ice ...
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bioRxiv - Immunology 2019Quote: ... non-specific binding of immunoglobulin to Fc receptors was blocked by anti-mouse CD16/32 antibody (BD Biosciences). Cells were stained in FACS buffer (PBS without Ca2+/Mg2+ supplemented with 2% FBS and 2 mM EDTA ...
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bioRxiv - Immunology 2021Quote: ... and non-specific binding was blocked by adding 5% (v/v) normal donkey serum and Fc block (BD). Avidin was used to neutralize endogenous biotin ...
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bioRxiv - Microbiology 2021Quote: ... After pre-incubation of samples with purified rat anti-mouse CD16/CD32 antibody (Mouse BD Fc Block) (BD Biosciences ...
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bioRxiv - Neuroscience 2021Quote: ... The remaining myelin-depleted cell suspension was blocked for ten minutes with Fc preblock (CD16/ CD32, BD 553141) on ice and stained for 20 minutes with antibodies to distinguish brain endothelial cells (CD31+/ CD45-) ...
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bioRxiv - Microbiology 2021Quote: ... The cells were blocked by adding 5µg/ml purified rat anti-mouse CD16/CD32 (mouse BD Fc Block) clone ...
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bioRxiv - Cancer Biology 2021Quote: ... The purification efficacy was analyzed by FC using the following surface markers: anti-CD45/PE antibody (BD Bioscience), anti-CD56/BB515 antibody (BD Bioscience ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed twice in PBS + 2% FCS + 2 mM EDTA and incubated with mouse FcBlock (BD Biosciences), Epcam-APC (CD326 Monoclonal Antibody (G8.8) ...
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bioRxiv - Microbiology 2022Quote: ... and Fixable Viability Dye eFluor 780 (Thermo Fischer) in presence of FC Block (BD, 20 min, 4°C). Stained cells were washed in FACS buffer twice and fixed in 2% paraformaldehyde (30 min ...
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bioRxiv - Immunology 2019Quote: ... Following FACS sorting the individual blood and tissue-derived subsets were incubated with Fc block reagent (BD Biosciences) and Sample Tag antibodies for 20 min at room temperature ...
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bioRxiv - Immunology 2020Quote: ... Without additional washing, an antibody cocktail containing Fc block (14-0161-86, eBioscience) BV buffer (566349, BD Bioscience) were added ...
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bioRxiv - Bioengineering 2019Quote: All antibody cocktails contained 1:100 dilution of anti-mouse Fc Receptor block CD16/CD32 (BD Biosciences 553141). Primary antibodies against H2-M3 was detected with secondary antibody conjugated to Alexa568 (Thermo Fisher A21112 ...
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bioRxiv - Cancer Biology 2019Quote: ... Samples were blocked for 10 minutes on ice with 1:100 diluted FC Block (BD Pharmingen, Clone 2.4G2) and metal-conjugated antibody cocktail added and incubated for 30 min at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Surface antibody staining was done in PBS containing 1% FCS and 1X Brilliant Stain Buffer (BD Biosciences, USA) for 20 minutes at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were stained for 45 min on ice in FC buffer containing unlabelled CD32 (BD Biosciences, Sydney, Australia) to block Fc receptor binding and HIS48-FitC ...
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bioRxiv - Cell Biology 2020Quote: ... cells were pre-incubated with anti-mouse CD16/32 mouse Fc blocker (BD Biosciences, San Jose, CA, USA) prior to staining with the antibodies ...
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bioRxiv - Immunology 2020Quote: ... Cells were incubated with Fc block and surface stained with anti-CD4 BUV805 (BD Biosciences; clone: RM4-5) or CD4 APC-Alexa Fluor 780 (eBioscience ...
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bioRxiv - Immunology 2020Quote: ... Cells were incubated for 15 min at 4°C with Fc-block (anti-CD16/CD32, 2.4G2, BD biosciences) before staining ...
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bioRxiv - Cancer Biology 2022Quote: The following anti-mouse antibodies were used following manufacturer’s instructions: anti-CD16/CD32 Fc blocking antibody (BD Biosciences), anti-CD24-FITC (clone M1/69 ...
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bioRxiv - Immunology 2022Quote: ... and were resuspended in FC stain buffer prior to data collection via an LSR Fortessa flow cytometer (BD). Analysis was done using FlowJo software (version 10.7.1) ...
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bioRxiv - Neuroscience 2023Quote: ... Fc receptors were blocked with 100 mL solution containing anti-mouse CD16/CD32 (1:100, BD Pharmingen, 553141) for 30 minutes at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Monocytes were sorted into HBSS-2% FCS in 15ml Falcon tubes using a BD Aria III (BD Biosciences). Monocytes were gated as Lineage-negative (B220 ...
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bioRxiv - Immunology 2023Quote: ... Cells were stained for 45 min on ice in FC buffer containing unlabelled CD32 (BD Biosciences, Sydney, Australia) to block Fc receptor binding ...
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bioRxiv - Microbiology 2022Quote: ... Single cell suspensions stained in PBS + 2% FBS in the presence of Fc receptor blocking antibody (BD Pharmingen) and stained with the antibodies against the following mouse proteins ...
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bioRxiv - Microbiology 2023Quote: ... and Fixable Viability Dye eFluor 780 (Thermo Fischer) in presence of FC Block (BD, 20 min, 4°C). Stained cells were washed in FACS buffer twice and fixed in 2% paraformaldehyde (30 min ...
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bioRxiv - Immunology 2023Quote: ... Cells were Fc-blocked with anti-mouse CD16/CD32 (1:100, Cat# 553141, BD Biosciences, San Jose, CA) followed by surface marker staining with antibodies to MerTK (2B10C42 ...
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bioRxiv - Immunology 2023Quote: ... and incubated for another 15 minutes at 4°C in the presence of Fc receptor block (BD Biosciences). Antibody cocktails for surface proteins were prepared in PBS + 2% FBS and Brilliant Stain Buffer (50 µl/100 µl of cocktail ...
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bioRxiv - Cell Biology 2023Quote: ... Fc receptors were blocked with anti-CD16/32 in the antibody staining solutions (BD Biosciences, Franklin Lakes, NJ). For RPE and endothelial identification ...
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bioRxiv - Cell Biology 2023Quote: FC analyses of SkM single cell suspensions were performed with the cell analyser LSRFortessa X-20 (BD Bioscience) using the FACSDiva 8.0 software ...
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bioRxiv - Microbiology 2022Quote: ... Housekeeping protein β-actin (a cytoskeleton protein; dilution, 1:5,000; BD Biosciences) was used as loading controls ...