Labshake search
Citations for Becton, Dickinson and Company :
901 - 950 of 990 citations for N BOC 3 FLUORO L TYROSINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Confluent definitive endoderm cells are then passed 1:3 in presence of Rock Inhibitor on growth factors reduced Matrigel (BD Biosciences) and cultured for 8 days in differentiation medium (DMEM F12 ...
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bioRxiv - Systems Biology 2024Quote: ... freshly sorted lineage-labeled Dclk1+ cells were resuspended in LPM-3D medium (LPM-3) and mixed with growth factor-reduced Matrigel (BD Biosciences) in a 1:1 ratio ...
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bioRxiv - Plant Biology 2024Quote: ... The dry volume (DV, m3) was measured by water displacement (Berta et al., 2010) and basic density (BD, kg m-3) calculated as reported by Carriero et al ...
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bioRxiv - Cell Biology 2024Quote: Cells were seeded 24 h before treatment and were allowed to grow in the presence of 3 μg/mL BrdU (BD Pharmingen) for 48 h ...
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IpaA reveals distinct modes of vinculin activation during Shigella invasion and cell-matrix adhesionbioRxiv - Cell Biology 2023Quote: ... NJ) were coated for 3 hours with 10 μg of Matrigel following the manufacturer’s instructions (Biocoat, BD Biosciences, San Jose, CA). Inserts were placed into 24-well dishes containing 500 μl of RPMI medium supplemented with 1% fetal calf serum ...
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bioRxiv - Cancer Biology 2023Quote: ... Flow cytometry was performed on a 3 laser Cytek Aurora Cytometer (Cytek Biosciences, Fremont, CA) and analyzed using FlowJo software (BD Biosciences). All antibodies are from Biolegend unless otherwise specified.
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bioRxiv - Immunology 2024Quote: ... and 5×104 of each were combined in 96-well U-bottom plates with anti-CD40 (100 ng/mL, clone HM40-3, BD Biosciences) in the presence or absence of OVA (10 μg/mL ...
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bioRxiv - Biochemistry 2023Quote: ... 1 µg of this genomic DNA from soluble fraction was mixed with 3 µg of anti-IdU/BrdU antibody (3D4 clone, BD Science) in BrdU-IP buffer composed of PBS and 0.0625% Triton X-100 overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... mice received a subcutaneous injection of 3 million VCaP cells in a mix of serum-free medium and 50% Matrigel (BD Biosciences). Once tumors grew to about 200 mm³ ...
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bioRxiv - Cell Biology 2023Quote: ... After 1 h of incubation at 37 °C the cells were washed 3 times with HBSS followed by trypsinization and signal was measured using FACScan and FACSCalibur systems (BD Biosciences). Data was analyzed with FlowJo™ v10 Software (BD Biosciences).
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bioRxiv - Immunology 2023Quote: ... with 3×104 PFU or injected intracranially (10uL) with 50 PFU of LGTV-TP21 using insulin syringes (BD Medical, #BD-329461). At appropriate times post infection ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibody incubation was overnight at 4 degrees C with the following antibodies at 1:100 dilution in PBS + 3% BSA: CD24-FITC (clone ML5, BD Bioscience), CD271-APC (clone C40-1457 ...
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bioRxiv - Cancer Biology 2022Quote: ... Percentage of caspase-3/7 negative cells in each treatment condition was normalized to vehicle-treated control populations (FlowJo software v9, BD Biosciences).
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bioRxiv - Biophysics 2022Quote: ... #131-8; #130-6; #130-5; #130-3 and 2 μm lot MM2307#156; #159.1; #159.2; #122.3, BD Biosciences, San Jose, CA). For calibration of the fluorescence axis in the PE channel ...
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bioRxiv - Cancer Biology 2023Quote: ... Flow cytometry was performed on a 3 laser Cytek Aurora Cytometer (Cytek Biosciences, Fremont, CA) and analyzed using FlowJo software (BD Biosciences). All antibodies are from Biolegend ...
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bioRxiv - Immunology 2023Quote: ... and ionomycin (500 ng/mL) for 3 hrs at 37°C prior to surface and intracellular staining using the BD Cytofix/Cytoperm Kit (BD Biosciences). Intracellular transcription factor staining was performed using a Foxp3 staining kit (ThermoFisher) ...
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bioRxiv - Microbiology 2023Quote: ... cells were incubated with fluorochrome-conjugated monoclonal antibodies (mAbs) diluted at 1:200 into a 1:3 solution of Brilliant Staining Buffer (BD 563794): Cell Staining Buffer ...
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bioRxiv - Immunology 2023Quote: ... and 1:20 dilution mouse anti-human CD123 (IL-3)-PE (stock 200 ug/mL, BD Biosciences, Franklin Lakes, NJ, USA). A 1:1,000 dilution of anti-mouse IgG Alexa-647 (stock 1 mg/mL ...
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bioRxiv - Cell Biology 2023Quote: ... E8.5 embryos were fixed with 4% paraformaldehyde (PFA) in PBS at 4°C for 3 h and incubated with FITC-conjugated anti-EEA1 (1:100, BD Bioscience) or Cy3-labeled anti-mouse IgG (1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... The cells followed by an incubation with the SM/Chol probe OlyA (3 µM) and primary anti-IFN-γR1 antibody (1:50) (BD Biosciences) together in 1% BSA-PBS at room temperature for 90 min ...
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bioRxiv - Immunology 2024Quote: ... 50 μl of PBS-BSA containing 2.5 μl of CD11c APC antibody (clone S-HCL-3) or 5 μl of CD11c BV421 antibody (clone B-ly6 RUO) (BD Biosciences) were added to each tube ...
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bioRxiv - Immunology 2024Quote: ... Flow cytometry was performed on a 3 laser Cytek Aurora Cytometer (Cytek Biosciences, Fremont, CA) and analyzed using FlowJo software (BD Biosciences). All antibodies are from Biolegend ...
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bioRxiv - Bioengineering 2024Quote: ... 25,000 irradiated HM-1-luc cells were mixed with 3 × 105 PBMCs per sample and seeded in a 96-well ELISPOT plate (BD Biosciences) that was pre-coated with IFN-γ capture antibody (BD Biosciences) ...
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bioRxiv - Microbiology 2024Quote: ... Cells were activated for 3 days with 1 μg/mL/million cells of purified NA/LE mouse anti-human CD3 and CD28 (BD Biosciences) and infected with HIVBaL at 0.01 ng/106 cells ...
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bioRxiv - Immunology 2024Quote: ... coated plates were blocked with PBS–3% rat serum and incubated with serum samples and with a biotinylated rat antimouse IgE (clone R35–118; BD Biosciences). Avidin-HRP (Ebiosciences ...
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bioRxiv - Cancer Biology 2023Quote: DOXR cells were seeded in a 10-mm cell culture dish for 1 day and then stained with 5 μg/mL of Hoechst-33342 at 37 L for 5 min and analyzed on a FACSAria™ Fusion (BD Biosciences, Franklin Lakes, NJ, USA).
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Planarian CREB-binding protein (CBP) gene family regulates stem cell maintenance and differentiationbioRxiv - Developmental Biology 2020Quote: ... which was incubated for 2 h in the dark at 37°C with 20 µM caspase-3 substrate Ac-DEVD-AMC (BD Biosciences Pharmingen) or 2 ml from a stock of 1 mg/ml for a final volume of 150 µl ...
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bioRxiv - Developmental Biology 2022Quote: Zebrafish wild-type and vsxKO retina sections from different developmental stages were analysed for the detection of apoptotic and mitotic cells using rabbit anti-active caspase-3 antibodies (BD Biosciences, 559565) and rabbit anti-phospho-histone H3 antibodies (Merck Millipore ...
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bioRxiv - Immunology 2021Quote: ... cells were stained for surface markers (when required) then fixed/permeabilized by using PE Active Caspase-3 Apoptosis Kit (BD Bioscience, 550914) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... The cell pellet was then resuspended in 1 ml of T-R minimal medium and injected into the IsoCaRB system using a 3 ml syringe (BD Biosciences # 309657) and a 20-gauge needle (Sigma-Aldrich #Z192511).
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bioRxiv - Molecular Biology 2021Quote: ... Between 2 - 4x105 events were collected per sample within 3 hours after staining on an LSRFortessa (BD Biosciences, 4 lasers, 18 parameters) and analyzed using FlowJo software (version 9.7.4 ...
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bioRxiv - Cancer Biology 2020Quote: ... Mammary fat pad injections were performed in the 4th pre-cleared mammary fat pad of 3 weeks old female mice in 50% matrigel (BD Biosciences, Germany). Breast or prostate cancer-origin of xenografts were verified by a pathologist.
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bioRxiv - Cancer Biology 2019Quote: ... Parental P18T and RAS pathway mutant knock-in patient-derived organoids were transplanted subcutaneous as single cells at a density of 3×10^5 organoids in 100 µl 50% Matrigel/medium with 10% collagen type I (BD Bio-sciences) mixture into NSG-B2m mice (JAX stock no ...
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ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactionsbioRxiv - Microbiology 2021Quote: ... Lentivirus was titrated by serial dilution of lentivirus and incubating with 5000 HEK293T cells in 96 well plates with 8 µg/ml polybrene for 3 days followed by GFP detection by flow cytometry (BD Biosciences LSRFortessa). Transduction units (TU ...
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bioRxiv - Immunology 2022Quote: ... 1x105 tumor cells and 1x105 γδ T cells were co-cultured for 3 h at 37°C in the presence of 1 µl α-CD107a-PE antibody (BD Biosciences). Medium or stimulation with α-CD3 and α-CD28 (both 1 µg/ml ...
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bioRxiv - Immunology 2022Quote: ... PBMC from the 3-time point from each patient were labelled using Single Cell Labelling with the BD Single-Cell Multiplexing Kit (BD Biosciences, #633781) and 52 different BD AbSeq Ab-Oligos reagents following the manufacturer’s protocol (BD Biosciences) ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were suspended in 500 μl PBS with 3 % FBS and then detected by flow cytometry (BD Biosciences, San Diego, CA, USA).
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bioRxiv - Molecular Biology 2019Quote: Whole-mount retinas were blocked with horse serum 4°C overnight then incubated at 4°C for 3 days with primary antibodies: Mouse anti-Oct4 (1:100, BD bioscience, 611203), Rabbit anti-Sox2 (1:100 ...
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bioRxiv - Molecular Biology 2019Quote: ... Four times washing in 500 μl 1x PBS (1,150xg, 3 min) prepared the fixed cells for quantitative analysis by FACS (BD FACSCanto, BD Biosciences). The fluorescent MitoTracker dye was detected using the mCherry channel (excitation ...
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bioRxiv - Neuroscience 2020Quote: COs were washed 3 times for 5 minutes in 5 mL of PBS in 5 mL round bottom tubes (BD Falcon, #1152367) then fixed overnight in 4% PFA at 4°C on a rocker ...
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bioRxiv - Immunology 2022Quote: T cells were isolated as described above from the intestine of 3 individual samples and PLZF+ CD4+ TCR-β+ T cells were sorted on proxy surface markers using FACSAria2 SORP (BD Biosciences) as previously described (14) ...
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bioRxiv - Molecular Biology 2022Quote: ... The dermis was incubated in the enzyme mix from the whole skin dissociation kit (Milteney Biotec) for 3 hours according to the manufacturer’s instructions and further processed by Medicon tissue disruptor (BD Biosciences, Stockholm, Sweden). The dermal cell suspension was incubated with CD90 Microbeads ...
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bioRxiv - Cell Biology 2022Quote: ... maintained for 20 min on ice and then homogenized with syringe every 5 min (BD Microlance™ 3 Needle 0,5 × 25 mm). Nuclear lysate supernatants were collected at 1000 rpm for 10 min at 4°C 43.
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bioRxiv - Physiology 2022Quote: ... Venous peripheral blood (4-6 ml for adults, 1- 3 mL for children and infants) was collected on-site on EDTA (BD vacutainer©) and immediately stored at 4-8°C until use ...
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bioRxiv - Immunology 2022Quote: ... Intracellular staining was carried out with the following antibodies diluted in BD Perm/Wash buffer: Active-caspase-3-GranzymeB-PE (C92-605, BD, cat #561011), Active-caspase-3-BV650 (C92-605 ...
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bioRxiv - Cancer Biology 2022Quote: ... The cells were stained for 30 min on ice with 5 μl of biotin-labeled anti–cleaved caspase-3 monoclonal antibody (BD Biosciences, #550821). The cells were washed in Perm/Wash buffer (BD Biosciences ...
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bioRxiv - Neuroscience 2024Quote: ... N2a-ssGT cells were imaged for GFP fluorescence using CytationTM 3 Cell Imaging Multi-Mode Reader (BioTekTM) and flow cytometry analysis by Accuri® C6 flow cytometer (BD Biosciences).
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bioRxiv - Microbiology 2024Quote: ... and HIV-1 NL4-3-GFP-I virus by plating 1.5 × 106 cells per well of a 6-well flat-bottom plate (BD Bioscience, X, Y) and adding 250 mU RT (equivalent to 45 ng p24 ...
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bioRxiv - Microbiology 2024Quote: ... as well as HIV-1 NL4.3-GFP-I virus by plating 1.5 × 106 cells per well of a 6-well flat-bottom plate (BD Bioscience, X, Y) and adding 250 mU RT (equivalent to 45 ng p24 ...
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bioRxiv - Cancer Biology 2024Quote: ... Flow cytometry was performed on a 3 laser Cytek Aurora Cytometer (Cytek Biosciences, Fremont, CA) and the data was analyzed using FlowJo software (BD Biosciences, RRID:SCR_008520). All listed antibodies for flow cytometry are from Biolegend ...