Labshake search
Citations for Becton, Dickinson and Company :
901 - 950 of 1633 citations for Galectin 7 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2023Quote: ... mouse anti-human CD15 conjugated to PE/Cyanine7 (clone HI98, BD, 560827), mouse anti-human CD33 conjugated to PE/Dazzle 594 (clone WM53 ...
-
bioRxiv - Bioengineering 2024Quote: ... non-specific antibody staining was blocked with Human Fc Block (BD Biosciences) for 5 min at 4°C ...
-
bioRxiv - Cancer Biology 2023Quote: ... mouse anti-human CD25 conjugated to BV650 (clone M-A251, BD, 563719), mouse anti-human CD11b conjugated to BV395 (clone ICRF44 ...
-
bioRxiv - Microbiology 2024Quote: ... APC-H7-coupled mouse anti-Human CD80 (BD Biosciences, 561134; 1/50), BB515-coupled mouse anti-Human PD-L1 (BD Biosciences ...
-
bioRxiv - Microbiology 2024Quote: ... BB515-coupled mouse anti-Human PD-L1 (BD Biosciences, 564554; 1/25), PE-Cy7-coupled mouse anti-Human ICOSL (BioLegend ...
-
bioRxiv - Cell Biology 2024Quote: Human BCAT1 (cat # 611271) and HIF1α (cat # 610958) antibodies were from BD Biosciences ...
-
bioRxiv - Cell Biology 2024Quote: ... purified mouse anti-human CD3 (clone HIT3a, BD Biosciences, Franklin Lakes, NJ); goat F(ab’)2 anti-mouse IgG-PE (Southern Biotech ...
-
bioRxiv - Immunology 2024Quote: ... anti-mouse/anti-human IL5-Allophycocyanin (APC) (BD Biosciences, #554396, clone TRFK5), IL13 monoclonal antibody ((eBio13A) ...
-
bioRxiv - Developmental Biology 2019Quote: ... Cells were resuspended in ice cold PBS and supplied with 10 μl of 7-AAD to assess cell viability (BD Pharmigen) and sorted with a BD Aria III FACS as previously described 30 ...
-
bioRxiv - Molecular Biology 2021Quote: ... Cells were then left unstimulated or stimulated with 50 ng/mL PMA and 1uM Ionomycin for 7 hours in the presence of a protein transport inhibitor (BD GolgiPlugTM, BD Biosciences). To stain for intracellular cytokines cells were washed with PBS supplemented with 3% FBS followed by a fixation and permeabilization step with FIX & PERM Kit (Invitrogen ...
-
bioRxiv - Microbiology 2020Quote: ... Adherent and suspension cells of each sample were pooled and after centrifugation for 5min at 800 x g cells were carefully resuspended in cell culture medium with 10 µl/ml 7-AAD (BD Biosciences). After 10 minutes incubation in the dark cells were immediately analyzed by flow cytometry using a FACS Aria III (BD Biosciences ...
-
bioRxiv - Cell Biology 2021Quote: ... washed twice and resuspended in 200 μL of blocking buffer in the presence of 7-AAD (BD Biosciences Cat#: 51-68981E) or DAPI (Invitrogen Cat# ...
-
bioRxiv - Cell Biology 2022Quote: ... Cell survival was measured by FACS analysis after staining cells with a membrane-impermeable DNA binding dye (DAPI, PI, or 7-AAD) and/or annexin V (BD Pharmingen).
-
bioRxiv - Developmental Biology 2022Quote: ... Cells from mouse tail skin (aging RNA-seq) and dorsal/ventral skin (fibulin 7 RNA-seq) were isolated using FACS Aria flow cytometer (BD Biosciences). Data were analyzed using FlowJo software (BD Biosciences).
-
bioRxiv - Cell Biology 2022Quote: ... Myoblasts were stained with 7-AAD (cell viability) and with the positive selection marker anti-CD56 AF647-conjugated (clone R19-760, BD biosciences). Of note ...
-
bioRxiv - Immunology 2020Quote: ... The cells were then stained with 7-AAD (live/dead marker) prior to flow cytometric sorting on an FACS Aria II sorter (BD Biosciences). The sorted germinal centre B cells (7-AAD−/B220+/GL-7+/Fas+ ...
-
bioRxiv - Immunology 2020Quote: ... A fixable Viability Dye (APC-eFluor780 1:200, 1:400) (eBioscience, Germany) or ViaProbe (7-AAD, 1:33) (BD Biosciences, Germany)) was used for live/dead discrimination ...
-
bioRxiv - Immunology 2021Quote: ... and then labeled with conjugated antibodies to surface antigens (PerCP-Cy®5.5 anti-mouse CD3, FITC anti-mouse CD4, APC-Cy®7 mouse anti-CD8, all from BD Biosciences ...
-
bioRxiv - Immunology 2021Quote: Mononuclear cells were directly labeled with conjugated antibodies to surface antigens (PerCP-Cy®5.5 anti-mouse CD3, clone, FITC anti-mouse CD4, APC-Cy®7 anti-mouse CD8, for T lymphocyte phenotype, all from BD Biosciences ...
-
bioRxiv - Genomics 2020Quote: ... ODInCas9 system was left to turn off for 7 days post transfection before assessment using FACS instrument BD LSRFortessa (BD Bioscience) and confocal microscopy instrument Cell Voyager 7000S (CV7000S ...
-
bioRxiv - Immunology 2019Quote: ... NK92 cells or primary patient fibroblasts were incubated with BrdU for 12 hours prior to fixation and staining with 7-AAD (BD Biosciences). Cells were analyzed via FACS on a BD Fortessa with 18-color configuration and resulting data were evaluated using Kaluza (Beckman-Coulter ...
-
bioRxiv - Microbiology 2021Quote: ... The infectivity was measured by detecting GFP fluorescence using a Zeiss fluorescence microscope (AXIO observer 7) and measured as the percentage of GFP positive cells analyzed with a Fortessa X20 flow cytometer (BD Biosciences) at 24 hours post infection ...
-
bioRxiv - Immunology 2020Quote: ... then washed with 2% FCS - PBS and stained with Mouse anti-IRF-7-PE (pS477/pS479, clone K47-671; BD Biosciences) or Mouse anti-NF-κB p65-Alexa Fluor 647 (pS529 ...
-
bioRxiv - Cell Biology 2021Quote: ... macrophages were incubated with 5 μM 2’, 7’ dichlorofluorescein diacetate (DCFDA, Invitrogen at 37°C for 30 min, followed by acquisition using FACS Canto I (BD Sciences) within 30 minutes of staining ...
-
bioRxiv - Cell Biology 2019Quote: ... Cells with normal mCherry fluorescence (mid 70-80% fluorescence) and top or bottom ~7% eGFP fluorescence (~4 million cells/population) were sorted using a BD FACSAria III (BD Biosciences) and collected into 15 ml polypropylene tubes (Cellstar ...
-
bioRxiv - Immunology 2020Quote: ... Spleen B cells were analyzed for BrdU incorporation (by anti-BrdU mAb staining) and DNA contents (by 7–AAD staining after cells were permeabilized) using the APC BrdU Flow kit® (BD) following the manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: ... infarct macrophages as CD45+/CD11b+/Ly6G−/F4/80+ cells and infarct neutrophils as CD45+/CD11b+/F4/80−/Ly6G+ cells.7–9 The following antibodies were used: anti-CD45 (clone 104, 1:100, BioLegend/BD Biosciences); anti-F4/80 (clone BM8 ...
-
bioRxiv - Microbiology 2021Quote: ... Infectivity was assessed by detecting GFP fluorescence using a Zeiss fluorescence microscope (AXIO observer 7) and/or determined by the percentage of GFP(+) cells analyzed using a Fortessa X20 flow cytometer (BD Biosciences) at 16 ...
-
bioRxiv - Neuroscience 2024Quote: ... OPs were obtained by immuno-panning from single-cell suspensions of dissociated P6-7 mouse cortices using anti-PDGFRA immunoglobulin (558774, BD Pharmingen) as previously described (Emery and Dugas ...
-
bioRxiv - Immunology 2024Quote: ... Cell apoptosis was measured by co-staining cells with Annexin V and 7-AAD using the PE Annexin V Apoptosis Detection Kit I (BD Biosciences), according to manufacturer’s protocol.
-
bioRxiv - Biochemistry 2023Quote: ... for all experiments except for experiment with soluble Siglec-7-Fc constructs in which a 5-laser Fortessa X-20 (BD Biosciences) was used instead.
-
bioRxiv - Microbiology 2023Quote: ... Cells were incubated for 7 days and immunostained for flow cytometry (Antibodies: CD34, clone 581; CD117, clone: 104D2; CD11b, clone ICRF44 (BD Pharmingen) and 1:50 FcR Blocking Reagent (Miltenyi Biotec)) ...
-
bioRxiv - Cancer Biology 2023Quote: Apoptosis was measured by evaluating the activation of caspase 3/7 using the Ac-DEVD-AMC Caspase-3 Fluorogenic Substrate (BD Biosciences) according to manufacturers’ instructions ...
-
bioRxiv - Biochemistry 2023Quote: Cells were grown in synthetic complete medium at 25°C to mid-log phase after 7-8 doublings and harvested for direct fluorescent measurement of RFP and YFP channels by an LSR Fortessa (BD biosciences) flow cytometer ...
-
bioRxiv - Cell Biology 2022Quote: ... 5 × 106 MCF-7 cells stably expressed shCtrl and shMYSM1 lentivirus were suspended in 100 μl of culture medium/Matrigel (1:1) (BD Biosciences) and implanted subcutaneously into the bilateral armpits of 4-week-old female BALB/c nude mice (Vital River Laboratory) ...
-
bioRxiv - Developmental Biology 2023Quote: ... or (ii) PE-Annexin V conjugated antibody (1:33 dilution in TBST) and 7-AAD as per kit instructions (BD Pharmingen). Samples were analysed by flow cytometry within 30 min.
-
bioRxiv - Cancer Biology 2022Quote: ... Percentage of caspase-3/7 negative cells in each treatment condition was normalized to vehicle-treated control populations (FlowJo software v9, BD Biosciences).
-
bioRxiv - Immunology 2023Quote: ... from naïve/PBS-treated or i.n fusion protein immunized mice were isolated from mLN from a pool of 7-8 C57Bl/6 mice and FACS-sorted on a Aria II cell sorter (BD Bioscience). Labeling of cells prior to sorting by flowcytometry (Fusion ...
-
bioRxiv - Microbiology 2023Quote: Bacteria used in this work are listed in Supplementary Table 7 and were grown at 37 °C in brain and heart infusion (BHI) broth (with agitation) and agar media (BD Difco). Cell lines of human origin used in this work included JEG-3 trophoblasts (ATCC HTB-36) ...
-
bioRxiv - Microbiology 2023Quote: ... The resulting tissue homogenates were each serially diluted from 10-1 to 10-7 and plated in triplicates onto Marine Agar 2216 (MA, BD Difco). Following one week of incubation at 23 °C ...
-
bioRxiv - Cancer Biology 2023Quote: ... and total DNA (7-AAD 1:50 solution dilution) and analyzed by flow cytometer in a BD LSRFortessa cell analyzer (BD Biosciences) using 488 nm and 640 nm lasers ...
-
bioRxiv - Molecular Biology 2024Quote: ... Nuclear extract was prepared by dounce homogenization in cold swelling buffer (using a size 7 dounce) and filtered using the cell strainer (100 μm, BD Biosciences). Lysate was centrifuged at 400 g for 10 min ...
-
bioRxiv - Cell Biology 2020Quote: ... Samples of human cell lines were then analyzed on a FACSCalibur (BD Biosciences) and primary murine samples were analyzed on a FACSCanto (BD Bioscience).
-
bioRxiv - Immunology 2020Quote: ... and APC mouse anti-human CD86 (1:20 dilution, Clone 2331, BD Pharmingen). The cells were subsequently washed and analysed on an Intellicyt® iQue Screener PLUS flow cytometer ...
-
bioRxiv - Immunology 2020Quote: ... PE mouse anti-human CD71 (1:20 dilution, Clone M-A712, BD Pharmingen) and APC mouse anti-human CD86 (1:20 dilution ...
-
bioRxiv - Developmental Biology 2020Quote: ... cells were incubated with PE-conjugated mouse anti-human CD44 or CD151 (BD) for 30 min at 4°C ...
-
bioRxiv - Synthetic Biology 2022Quote: ... PE-CF594 Mouse anti-human Akt pS473 clone M89-61 (BD Biosciences #562465), AF647 Mouse anti-human Akt pS473 clone M89-61 (BD Biosciences #560343).
-
bioRxiv - Immunology 2022Quote: Healthy human whole blood was collected by venous puncture into collection tubes (BD catalog# 364606 ...
-
bioRxiv - Immunology 2022Quote: ... Intracellular cytokine staining was performed with anti-human IFNγ and TNFα (BD Biosciences) antibodies for 30 min at room temperature ...
-
bioRxiv - Immunology 2021Quote: ... Anti-human CD45 PE-CF594 (1:100, clone HI30, BD Biosciences, cat # 562279), Anti-human CD29 PE Cy7 (1:100 ...