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Citations for Becton, Dickinson and Company :
901 - 950 of 1909 citations for BAFF TNFSF13B Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... cells were resuspended in FACS buffer and incubated in FC Block for 5 minutes (1:60, BD Pharmingen, 553142) and primary antibodies for 10 minutes ...
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bioRxiv - Immunology 2023Quote: ... and blocked unspecific bindings with using anti-mouse CD16/CD32 (Mouse BD Fc Block, BD Biosciences, Franklin Lakes, NJ). Cells were then stained with FITC-conjugated anti-mouse CD45 (BioLegend) ...
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bioRxiv - Immunology 2023Quote: Single-cell suspensions from tumors or spleens were incubated in Fc block (BD Biosciences, 553142, vol/vol 1/50) at room temperature for 10 min ...
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bioRxiv - Immunology 2024Quote: ... 1% FCS and 2mM EDTA) and stained for extracellular molecules with the following antibodies: anti-CD8a (53-6.7, BD), anti-CD4 (GK1.4 ...
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bioRxiv - Immunology 2024Quote: ... and Fc-receptors were blocked (2.4G2) before staining with monoclonal antibodies before collection on a Fortessa flow cytometer (BD). Fluorochrome-conjugated antibodies to the following cell surface molecules were used ...
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bioRxiv - Immunology 2024Quote: ... cells were blocked with 2% FBS and 0.2% goat serum containing HBSS including 1:1000 Mouse BD Fc Block™ (Clone 2.4G2, BDBiosciences). For detection of endogenous p65 ...
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bioRxiv - Immunology 2024Quote: ... Cells were blocked with 2% FBS and 0.2% goat serum containing HBSS including 1:1000 Mouse BD Fc Block™ (Clone 2.4G2, BDBiosciences) and stained with cell surface marker detecting fluorophore-conjugated antibodies ...
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bioRxiv - Immunology 2024Quote: ... and blocking with 2% FBS and 0.2% goat serum containing HBSS including 1:1000 Mouse BD Fc Block™ (Clone 2.4G2, BDBiosciences). Intracellular proteins were stained with fluorophore-conjugated antibodies overnight at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... PE mouse anti-human VCAM-1 or Alexa Fluor 647 mouse anti-human CD31 (all antibodies were from BD, Franklin Lakes, NJ, USA). Samples were analyzed on Gallios Flow Cytometer (Beckman Coulter ...
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bioRxiv - Cancer Biology 2022Quote: ... samples were thawed and sandwich ELISA for human or mouse IFN-γ levels was performed using the BD Human IFN-gamma ELISA Set (BD Biosciences, 555142) or BD Mouse IFN-gamma ELISA Set (BD Biosciences ...
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bioRxiv - Genomics 2019Quote: ... fluorescein isothiocyanate (FITC)- mouse anti-human Annexin-V (biolegend; Cat# 640945) and APC-anti-human anti-CD42b (GPIb) monoclonal antibody (BD Pharmingen; Cat# 551061). The calcein AM blue was used to identify live platelet sized events ...
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bioRxiv - Cell Biology 2020Quote: ... resuspended in 100 μl PBS/5% FBS and blocked with 2.5 μg of Fc-blocking solution (BD Bioscience, Cat #564220) for 15 min at room temperature ...
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bioRxiv - Immunology 2021Quote: ... cells were either resuspended in 400μl of PBS 2%FCS 2mM EDTA and analyzed on Fortessa-X20/Symphony cytometers (BD Biosciences) or in RPMI media and used for cell sorting on a FACSAria II (BD ...
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bioRxiv - Neuroscience 2021Quote: ... The cells were incubated with Fc block (eBiosciences 16-0161-81) for 5 min then incubated with antibodies (CD45-APC BD Biosciences 561018 ...
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bioRxiv - Neuroscience 2021Quote: ... The cells were incubated with Fc block (eBiosciences 16-0161-81) for 5 min then incubated with antibodies (CD45-APC BD Biosciences 559864 ...
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bioRxiv - Neuroscience 2020Quote: ... and microglia (CD45med CD11b+were sorted into collection tubes coated with FCS and filled with complete RPMI 1640 medium (1% penicillin, streptomycin, 0.1% 2-ME) with 20% FCS using a FACSAria device (BD Biosciences). Purity of sorted populations was routinely above 95% ...
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bioRxiv - Microbiology 2021Quote: ... samples were re-suspended in 25 μl of anti-mouse CD16/CD32-Fc/Block (BD Bioscience, San Jose, CA, USA), incubated 15 minutes on ice and then stained at RT in the dark for 20 minutes with the following mixture of fluorescent-labeled antibodies ...
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bioRxiv - Microbiology 2021Quote: ... Pellets were resuspended in 300µL of PBS + 1% v/v FBS and 150µL were added to 2µL of Mouse BD Fc Block (BD Biosciences 553142). Homogenates in Fc block were incubated for 15 minutes at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Single cell suspensions for myeloid panel staining were incubated in Fc Block (Cat#553142, BD Biosciences, Franklin Lakes, NJ, USA). Fluorescent minus one staining controls were used for both panels where required ...
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bioRxiv - Immunology 2022Quote: ... Fc-blocked (1 μg/mL; eBiosciences) BALF cells were stained with anti-mouse SiglecF-PE (0.3 μg/mL; BD Pharmingen), CD11c-APC (0.3 μg/mL ...
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bioRxiv - Immunology 2022Quote: For RNAseq analysis BM cells were isolated by flushing the long bones with FACS buffer (2mM EDTA, 1% FCS, PBS) and treated with Pharm LyseTM buffer (BD). Cells were enriched by magnetic removal of CD11b+ and CD19+ cells (EasySepTM ...
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bioRxiv - Microbiology 2022Quote: ... For each sample 1.5 x 106 cells first underwent Fc receptor blockade with rat anti-mouse FcγRIII/II receptor (CD16/32; BD Biosciences). After Fc receptor blocking for 5 minutes on ice ...
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bioRxiv - Immunology 2022Quote: Ex vivo isolated T cells and in vitro-cultured T cells were incubated with Fc-block (1:250; BD Biosciences) followed by surface staining ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were then washed with FC buffer (PBS supplemented with 2% HI-FBS and 0.05% NaN3) and resuspended in Fixation Buffer (BD Pharmingen)for 5 min at 4C° in the dark ...
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bioRxiv - Cancer Biology 2022Quote: ... Mouse cells were incubated with mouse BD Fc BlockTM containing purified anti-mouse CD16/CD32 mAb 2.4G2 at 1:400 (553142, BD BioSciences) for 10 min at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Isolated intestinal epithelial cells (107 cells/1ml) were incubated with Mouse BD Fc Block™ (55314, BD, Franklin Lakes, NJ) for 10 minutes on ice in IESC media (DMEM/F12 ...
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bioRxiv - Microbiology 2020Quote: ... and 3×106 cells per tube were stained as follows: cells were incubated in 0.5 μg Fc block (BD Biosciences) for 15 min at 4°C in staining buffer (Ca2+ - and Mg2+ - free PBS containing 3% heat-inactivated fetal bovine serum ...
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bioRxiv - Microbiology 2021Quote: ... Fc receptors were blocked using the anti-mouse CD16/CD32 (FcBlock clone 2.4G2) monoclonal antibodies (BD Biosciences, #553141, 1/400) and cells were stained for 30 min at 4°C with a combination of the following anti mouse immunophenotyping antibodies ...
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bioRxiv - Cancer Biology 2021Quote: ... Single-cell suspensions were stained with 5 μM Cell-ID Cisplatin (Fluidigm Corp.) and incubated with Fc block (BD Biosciences), followed by surface antibody cocktail ...
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bioRxiv - Neuroscience 2022Quote: ... Unspecific binding of antibodies was blocked by previous incubation for 10 min with anti CD16/CD32 (Fc block, clone 2.4G2; #55314, BD Pharmingen) in Fluorescence Activated Cell Sorting (FACS ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed and incubated (15-20 min., on ice) with Fc Block (1:100 in 1% FBS/PBS, Clone 2.4G2, BD Biosciences), followed by anti-CD45-PE-Cy5.5 (1:100 ...
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bioRxiv - Cell Biology 2020Quote: ... 3×106 cells were incubated with purified rat IgG2b anti-mouse CD16/32 receptor antibody as per supplier’s instructions (Mouse BD Fc-block, clone 2.4G2, BD Biosciences, 553141) for 20 minutes at 4°C ...
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Single cell transcriptomics reveals the effect of PD-L1/TGF-β blockade on the tumor microenvironmentbioRxiv - Genomics 2020Quote: ... The liquid was poured off from the cells (leaving approximately 100 µl in each tube) and 5 µl of mouse Fc Blocking Reagent (rat anti-Mouse CD16/CD32 BD) was added to samples and incubated for 5 minutes at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed in FACS buffer (PBS with 2% Heat-inactivated FCS) and fixed in 1x BD cellFIX (BD Biosciences). Samples were acquired on the LSRFortessa (BD Biosciences) ...
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bioRxiv - Immunology 2021Quote: Surface staining was done in the presence of anti-CD16/32 antibodies to block FcγRII/III receptors using Mouse Fc blockTM (BD Biosciences). Cells were incubated with the Mouse Fc blockTM for 10 min followed by incubation with fluorochrome-conjugated pre-titrated antibodies for 20 min at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Pellets were suspended in 100 μl of flow buffer and blocked with 0.25 μg Fc block (BD Pharmingen, CA, USA) for 10 min followed by staining with antibodies ...
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bioRxiv - Immunology 2020Quote: ... Samples were then washed with 2% and stained with 2% FCS in PBS and subsequently washed stained with goat anti-rabbit IgG –FITC secondary (BD) for 30 minutes at RT ...
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bioRxiv - Neuroscience 2019Quote: Cell pellets were eluted in FACs buffer (0.5% BSA in PBS with 2mM EDTA) and blocked for ten min with Fc preblock (CD16/CD32, BD 553141), followed by 20 minute staining with anti-CD31-APC (1:100 ...
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bioRxiv - Cell Biology 2020Quote: ... cells were resuspended in PBS with 1% FCS and analyzed for GFP and AF647 fluorescence on a FACS Verse (BD). Nontransfected and non-AF647-stained cells were used for gating the cells positive for GFP and AF647 ...
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bioRxiv - Immunology 2020Quote: ... Non-specific binding was blocked with 3% (w/v) bovine serum albumin (BSA, ChemCruz) in PBS with 0.5 g/mL Fc Block (BD Biosciences) for 30 minutes ...
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bioRxiv - Immunology 2021Quote: ... All samples were then incubated with Fc block (5 μg mL-1 CD16/CD32 (BD Biosciences, San Diego, CA, USA) and 0.1 % mouse serum in FACs buffer (PBS containing 0.5 % BSA and 2 mM EDTA (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... Cells were then treated with dasatinib for 1 hr at 37°C prior to blocking with Fc-block (BD Bioscience), 5 % normal mouse serum ...
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bioRxiv - Immunology 2021Quote: ... Surface antibody cocktail was prepared in cell staining buffer (Fluidigm) and added to all samples equally after Fc block (BD). Cells were washed then fixed and permeabilized using eBioscience Transcription Factor Fix/Perm Buffer followed by barcoding (Fluidigm) ...
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bioRxiv - Genomics 2021Quote: ... Cell counting was performed by counting of the events with FCS ≥ 1.104 and SSC ≥ 5.105 by flow cytometry (BD-Accuri-C6). The transposition activity was represented as the ratio of the hygromycine-resistant colonies to the total cells ...
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bioRxiv - Immunology 2022Quote: Single cell suspensions were first incubated for 10 minutes on ice with anti-mouse CD16/32 Fc Block (BD Biosciences), and subsequently stained with fluorophore-conjugated antibodies against surface markers at 4°C for 20 mins ...
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bioRxiv - Cancer Biology 2022Quote: ... The cells were then resuspended incubated with anti-mouse CD16/CD32 at 1:400 (BD Fc BlockTM, BD Biosciences #553142) for 10 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... The cells were then resuspended incubated with anti-mouse CD16/CD32 at 1:400 (BD Fc BlockTM, BD Biosciences #553142) for 10 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were counted and resuspended to 10×106 cells/100μl in 1:50 Rat Anti-mouse CD16/CD32 Moue Fc Block (BD Biosciences) in FACS buffer (3% FBS ...
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bioRxiv - Immunology 2022Quote: ... Red blood cells were lysed using ammonium chloride and Fc receptors were blocked using anti-mouse CD16/CD32 (BD Bioscience) before staining.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and resuspended in 100 μL of a 1:50 dilution of Mouse BD FC block (BD Pharmigen, San Joe, USA). Cells were then stained using the following fluorescent antibodies ...