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Citations for Becton, Dickinson and Company :
901 - 950 of 7852 citations for 6 Chloro 2 methyl 1 2 4 triazolo 4 3 b pyridazin 3 2H one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Membranes were blocked by incubation in 5% non-fat milk powder in 0.1% Tween 20 in Tris buffered saline (TBS-T) and incubated overnight at 4°C with primary antibodies: ALIX (BD Biosciences, 611620, 1:1000), calnexin (Santa Cruz ...
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bioRxiv - Microbiology 2019Quote: ... The cell pellet was then resuspended in 1 ml of T-R minimal medium and injected into the IsoCaRB system using a 3 ml syringe (BD Biosciences # 309657) and a 20-gauge needle (Sigma-Aldrich #Z192511).
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bioRxiv - Immunology 2022Quote: ... 1x105 tumor cells and 1x105 γδ T cells were co-cultured for 3 h at 37°C in the presence of 1 µl α-CD107a-PE antibody (BD Biosciences). Medium or stimulation with α-CD3 and α-CD28 (both 1 µg/ml ...
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bioRxiv - Cancer Biology 2021Quote: ... eBioscience] for 45 min at 4°C together with different additional antibodies for immunophenotyping (including 1:10 anti-CD103-FITC [clone Ber-ACT8, BD Biosciences] ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated with primary antibody in incubation buffer overnight at 4°C with mouse anti-CtBP2 antibody (1:50, BD Transduction ...
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bioRxiv - Immunology 2019Quote: ... and incubated for 30 min at 4°C with antibodies specific for the following markers: CD45.2-PE-Cy7 (1:200 dilution; BD Biosciences); EpCAM-APC (1:100 dilution ...
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bioRxiv - Immunology 2019Quote: ... Each obtained monomeric H-2Db/peptide complex (0.5 mg/ml) was tetramerized at a 4:1 ratio with streptavidin-PE (BD Biosciences).
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bioRxiv - Cancer Biology 2020Quote: ... Sections were then fixed in 4% PFA and incubated with primary antibodies: anti-CD31 (1:50, BD Pharmigen; RRID: AB_393571), anti-CD42b (1:75 ...
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bioRxiv - Neuroscience 2022Quote: ... with 0.2% Triton X-100 supplemented with 10% FBS and then incubated overnight at 4 °C with primary antibodies (1:100 mouse anti-ASCL1, BD 556604 ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated with primary (overnight at 4 °C) and secondary (1h at room temperature) antibodies (1:100 mouse anti-ASCL1, BD 556604 ...
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bioRxiv - Immunology 2022Quote: ... Cells were then stained with one of four antibody panels (Supplementary Table 1) assessing different immune populations and analysed by flow cytometry (4-laser LSR Fortessa, BD). Raw FCS files were analysed using FlowJo (v10.6.2 ...
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bioRxiv - Immunology 2020Quote: ... 1×106 cells were blocked for 20 min at 4°C with FcR antibody (purified anti-CD16/32; BD Pharmingen) diluted (1:50 ...
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bioRxiv - Pathology 2020Quote: ... slides were incubated overnight at 4 °C with a 1:100 dilution of N-cadherin antibody (BD Transduction Labs, #610920) followed by incubation with secondary fluorescence antibody Alexa Fluor Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... Cryosections were then incubated overnight at 4°C with primary antibody rat anti-mouse CD31 (BD PharMingen, PMG550274; 1:50). The next day ...
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bioRxiv - Immunology 2020Quote: ... media for 1 hour at 37°C and then an additional 4 hours with Brefeldin A (10 μg/mL) and GolgiStop (BD) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were probed overnight at 4°C with the following primary antibodies: anti-ALIX (AIP1) (1:250; BD Biosciences, 611621), anti-CD9 (1:1000 ...
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bioRxiv - Immunology 2022Quote: ... diluted 1:1000 in PBS for 30 min at 4 °C and stained with PE Rat Anti-Mouse CD45 antibody (BD) in FACS buffer for 30 min at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... Single cell suspensions were incubated for 15 minutes at 4°C with anti CD16/CD32 Mouse BD Fc Block diluted 1/50 (BD) in 100 µL of FACS buffer in 96 wells round-bottom plates (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... then incubated overnight at 4° with primary antibodies at the following dilutions: mouse anti-βII-spectrin 1:2000 (BD-bioscience), rabbit anti-βII-spectrin 1:2000 (Abcam) ...
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bioRxiv - Cell Biology 2023Quote: ... CECs were incubated overnight at 4°C with primary antibodies mouse monoclonal anti-CD166 [3A6] (1:200 dilution, BD Biosciences), rabbit polyclonal anti-ZO1 (1:100 dilution ...
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bioRxiv - Cancer Biology 2023Quote: ... High-titer viral solutions were prepared and used for transduction into both WT and NF1 KO BT-474 cells by two rounds of infection of 4 viral particles per cell (MOI 1) followed by flow cytometry-based sorting (FACSAria cell sorter, BD). For live cell imaging ...
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bioRxiv - Molecular Biology 2023Quote: ... were coated overnight at 4 °C with 300 ng/mL mouse anti-human RIPK1 capture antibody (1:100 dilution in PBS of BD Bioscience BD610459 lot 0314064 ...
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bioRxiv - Immunology 2023Quote: ... Surface antigens were stained for 30 min at 4°C with indicated antibodies (Table.1) after Fc blocking for 4°C 30 min with anti-mouse CD16/32 (BD) without wash ...
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bioRxiv - Molecular Biology 2024Quote: ... The cells were then incubated overnight at 4 °C with primary antibody against TIM23 (1:200; BD-611222; BD Biosciences) diluted in PBS supplemented with 5% FBS ...
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bioRxiv - Molecular Biology 2024Quote: ... The cells were then incubated overnight at 4 °C with primary antibody against TIM23 (1:200; BD-611222; BD Biosciences) diluted in PBS supplemented with 5% FBS ...
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bioRxiv - Bioengineering 2024Quote: ... The samples were stored in PBS at 4°C for up to 1 week in dark before flow cytometry analysis by LSRFortessa (BD). The data were analyzed by the WEASEL software (2.7 ...
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bioRxiv - Immunology 2024Quote: ... cell suspensions were surface labelled for 30 min at 4°C with CD3 BV785 (clone 17A2, 1:50, BD Horizon), CD8α BUV805 (clone 53-6.7 ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were rinsed with 2 × 2 ml Pharmingen stain buffer and fixed with 4.21% (w/w) formaldehyde (BD Biosciences, #554655). Cells were analyzed on LSRFortessa X-20 (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... All flow cytometry analyses were performed in FACS buffer containing PBS with 2 mM EDTA and 2% FBS on a FACS CANTO (BD Pharmingen) and analysed with FlowJo software (Treestar).
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bioRxiv - Microbiology 2023Quote: ... Membranes were blocked with 2% ECL prime blocking reagent in PBS-T (Amersham; #RPN418) and incubated against AP-2 (mouse-anti-AP50, BD, #A611351) and GAPDH (rabbit-anti-GAPDH ...
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bioRxiv - Microbiology 2023Quote: ... and values for IL-2 were determined by using a standard curve generated by using known concentrations of purified recombinant murine IL-2 (BD Biosciences).
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bioRxiv - Immunology 2023Quote: ... up to 2 x106 MR1-5-OP-RU-APC Tetramer magnetic bead enriched spleen (n= 2) or unenriched colon (n= 2) single cell suspensions were labeled with an oligonucleotide-tagged antibody sample tag (BD Biosciences). MAIT cells (MR1-5-OP-RU-Tet+TCRβ+CD3+ ...
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bioRxiv - Immunology 2023Quote: ... Samples were stored in PBS containing 2% (V/V) FBS and 2 mM EDTA until acquisition on an LSR Fortessa flow cytometer (BD Biosciences). Analyses were performed with FlowJo v10.7.1 (BD Biosciences).
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bioRxiv - Biophysics 2023Quote: ... Cells were then washed twice in FACS buffer (PBS, 2% FBS, 2 mM EDTA) and permeabilized in BD Phosflow Perm Buffer III (BD Biosciences), according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed with ice-cold FACS buffer (DPBS supplemented with 2% FBS and 2 mM EDTA) and subjected to fixation and staining with Cell Fixation and Permeabilization Kits (BD Biosciences). Incubation with antibodies was performed for 1h at RT ...
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bioRxiv - Neuroscience 2022Quote: ... Daigger Scientific) with a rounded tip connected to a blunted 30G x 1/2 needle (BD PrecisionGlide) and 1 cc syringe (Terumo ...
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bioRxiv - Neuroscience 2021Quote: Primary antibodies were obtained from the following sources: anti-ataxin-2 (mouse monoclonal, 1:500, BD biosciences) anti-ataxin-3 (mouse monoclonal 1H9 ...
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bioRxiv - Immunology 2023Quote: ... modifications to the protocol were made: 1 μl of 2 μM additive primer (BD Genomics, beta kit) specific to the antibodies tags was added to the amplification mixture ...
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bioRxiv - Immunology 2024Quote: ... the following monoclonal antibodies were used: anti-Human CD253 (TRAIL)-PE (RIK-2, 1:100, BD Biosciences), anti-Hu CD95 (APO-1/Fas)-APC (DX2 ...
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bioRxiv - Bioengineering 2019Quote: ... The cells were counted on FL-3 fluorescence channel using FACSCalibur flow cytometer (BD).
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bioRxiv - Molecular Biology 2020Quote: ... 500 μl cell suspension was dispensed in 3 ml FACS tubes (BD biosciences, USA). For cells treated with inhibitor 1 μM Ibrutinib was added directly to the cell suspension ...
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bioRxiv - Microbiology 2021Quote: ... transfectants were subjected to 3 weeks of pyrimethamine selection followed by FACS (BD FACSAriaIII) into ten 96-well plates ...
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bioRxiv - Neuroscience 2022Quote: ... About 3 × 105 nuclei were sorted by a BD FACSAria™ Fusion (BD Biosciences) into a collection tube that was precoated and filled with 1 mL of PBSE + BSA ...
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bioRxiv - Biochemistry 2020Quote: ... the membranes were washed and incubated with an antibody against SMAD2/3 (BD Biosciences) conjugated to Alexa 488 using the Zenon® Mouse IgG Labeling Kits (Life Technology ...
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bioRxiv - Immunology 2019Quote: ... Live NL4-3 ΔGPE-GFP transduced cells were sorted using a FACSAria III (BD) and gating on GFP+ cells.
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bioRxiv - Genomics 2021Quote: Peripheral blood (3-4ml) from cattle was collected into an EDTA tube (BD Vacutainer). An aliquot of the whole blood (600µl ...
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bioRxiv - Immunology 2020Quote: ... and analyzed 3 days later using flow cytometry on a LSR IIW (BD Biosciences). GFP Mean fluorescence intensity was calculated on live mCherry-positive cells and compared to empty vector control.
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bioRxiv - Cell Biology 2022Quote: ... Rehydrated tissues sections of 3 µm were incubated with anti-Ki67 antibodies (BD Pharmingen) and staining was visualized using Dako EnVision+ (Agilent Technologies ...
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bioRxiv - Cancer Biology 2022Quote: ... mixed with the adjuvants anti-CD40 (12.5 μg, Clone 3/23, BD Biosciences; 553787) and poly(I:C ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed 3 times in FACS buffer and incubated with Cytofix/Cytoperm (BD) for 1 hour on ice ...