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Citations for Becton, Dickinson and Company :
851 - 900 of 1557 citations for pIEXBac c EGFP 3 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... then washed and stored at 4°C in the dark until analysis using a FACSCelesta cell analyzer (BD Biosciences) configured with 405 nm ...
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bioRxiv - Microbiology 2022Quote: ... They were cultivated on PDA plates at 25°C for growth and in potato dextrose broth (BD Difco™) culture for genomic DNA extraction as previously reported41 ...
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bioRxiv - Immunology 2022Quote: ... washed with PBS and incubated for 30 minutes at 4°C in the dark with Brilliant Stain Buffer (BD) and the fluorescently-conjugated antibodies ...
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Glutamine synthetase mRNA releases sRNA from its 3’UTR to regulate carbon/nitrogen metabolic balancebioRxiv - Microbiology 2022Quote: ... Bacterial cells were grown at 37°C with reciprocal shaking at 180 rpm in LB Miller medium (BD Biosciences) or MOPS minimal medium (Neidhardt et al ...
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bioRxiv - Genetics 2020Quote: ... at 37°C / 5% CO2 for 15 h and subsequently incubated in the presence of brefeldin A (BD Biosciences) at 37°C with 5% CO2 for up to 4 h ...
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bioRxiv - Microbiology 2021Quote: ... The plates were incubated overnight at 37°C and then bacteria were harvested by adding 1.0 ml of Buffered Peptone Water (BPW, BD) to the surface of the plate and gently scraped using an L-shaped plate spreader ...
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bioRxiv - Synthetic Biology 2020Quote: ... Yeast strains were cultured at 30 °C in complex yeast extract peptone dextrose (YPD, all components from BD Diagnostics) medium or synthetic defined medium (SDM ...
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bioRxiv - Immunology 2020Quote: ... for 45 minutes at 4°C in FACS buffer containing 0.5 mg/mL anti-mouse CD16/CD23 (2.4G2) (BD Biosciences). Flow cytometry was performed on an LSR-II with BD FACSDIVA software (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... specific antibodies were bound with polyclonal anti-mouse Ig-FITC (1:300, 4°C, 20 min incubation; BD Biosciences), anti-mouse IgG1-APC (1:300 ...
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bioRxiv - Immunology 2020Quote: ... followed by intracellular staining (30 min at 4°C) using a cocktail of the following antibodies purchased from BD, Biolegend ...
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bioRxiv - Microbiology 2021Quote: Genomic DNA was prepared from all the isolates grown overnight at 37°C in tryptone soy broth (BD Biosciences), using bacterial DNA kits (Omega Bio-Tek) ...
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bioRxiv - Developmental Biology 2021Quote: ... The primary antibodies for flow cytometry were: rat anti-C-peptide-Alexa Fluor 647 (1:200, 565831, BD Pharmigen) and mouse anti-Glucagon-BV421 (1:100 ...
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bioRxiv - Microbiology 2020Quote: ... for 15 min at 37 °C and counted using a BD Accurri flow cytometer (BD Bioscience, Franklin Lakes, NJ). The ratio between alive and dead cells was calculated by staining the same diluted biofilm suspension using the LIVE/DEAD® BacLight™ Bacterial Viability and Counting kit (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2020Quote: ... lactis strains were grown at 30 °C in M17 liquid medium (BD - Becton, Dickinson and Company, Franklin Lakes, NJ) supplemented with 0.5 % glucose (GM17).
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bioRxiv - Biophysics 2022Quote: ... The tissue sections were incubated at 4 °C overnight with rat anti-mouse CD31 (cat. no. 553370, BD Biosciences) and rabbit anti-cleaved Caspase-3 (cat ...
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bioRxiv - Biophysics 2022Quote: ... cells were cultivated in HL5 medium (Formedium) at 22 °C on polystyrene Petri dishes (Primaria, Falcon, BD Becton Dickinson) and passaged every 2-3 days ...
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bioRxiv - Cell Biology 2022Quote: ... cells were blocked at 4°C for 15 min using anti-mouse CD16/CD32 antibody (1:250, BD Biosciences) before wash ...
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bioRxiv - Microbiology 2024Quote: ... Single colonies from sub-streaked plates were frozen down at −80°C in brain heart infusion broth (BHI, BD) containing 15% glycerol for long-term storage.
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bioRxiv - Immunology 2024Quote: ... cells were blocked at 4°C for 15 min using anti-mouse CD16/CD32 antibody (1:250, BD Biosciences). Next ...
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bioRxiv - Immunology 2024Quote: ... to identify viable cells and then stained for 45 minutes at 4°C with anti–mouse FcγRIII/ II (2.4G2; BD Pharmingen) and the following antibodies from BioLegend diluted in FACS buffer (PBS with 2% FBS) ...
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bioRxiv - Microbiology 2023Quote: ... and 1 mM IPTG for 48 hours at 30°C with shaking before spotting on MacConkey agar (BD Difco) plates.
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bioRxiv - Microbiology 2023Quote: ... Fn and ETBF were grown anaerobically at 37°C on Bacto™ Brain Heart Infusion Broth (BD, Sparks, MD) supplemented with 0.01% Hemin in 1M NaOH ...
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bioRxiv - Immunology 2023Quote: ... the cells were collected and stained for 20 min at 4°C with CD8 BV421 (RPA-T8, BD Biosciences). After cell permeabilization in cold 90% methanol for 2-3 days at -80°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Single GFP positive cells were sorted at 4°C using a BD FACSAria III (BD Biosciences, Franklin Lakes, NJ) and deposited in 1% gelatin-coated 96 well plates ...
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bioRxiv - Molecular Biology 2023Quote: ... a single bacterial colony from a freshly streaked plate was grown overnight at 37 °C in low salt LB medium (LS-LB: 1.0% bactotryptone (BD), 0.5% yeast extract (Alfa Aesar) ...
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bioRxiv - Neuroscience 2023Quote: ... and cardiac perfusion was performed with 100 ml of 4 C PBS (0.1 M, pH 7.2) placing a 25G 0.50 mm X 16 mm syringe (BD Vacutainer, 367294) through the left ventricle of the heart to remove blood from the brain and organs ...
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bioRxiv - Immunology 2023Quote: ... Cells were labeled for 20 min at 4 °C with the following specific antibodies: BUV496 anti-CD19 (SJ25C1;BD), BUV563 anti-CD56 (MY31;BD) ...
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bioRxiv - Immunology 2023Quote: ... and IELs were stained in 2.4G2 supernatant for 30 minutes at 4°C with the following antibodies: (BD Biosciences) CD4 (RM4-4) ...
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bioRxiv - Microbiology 2023Quote: Mycobacterium tuberculosis (Mtb) H37Rv was grown at 37°C in a minimal medium [Difco Middlebrook 7H9 broth (BD, 271310) supplemented with 0.5% (v/v ...
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bioRxiv - Cell Biology 2022Quote: ... Strains were grown at 30°C in synthetic complete medium SDC (0.7% (w/v) yeast nitrogen base (BD Difco) and 2% (w/v ...
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bioRxiv - Developmental Biology 2023Quote: ... dissociated cell clusters were fixed for 20 min at 4°C in cold BD fixation/permeabilizationTM solution (BD Bioscience). Cells were washed twice in BD Perm/WashTM Buffer (BD Bioscience ...
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bioRxiv - Immunology 2023Quote: ... LPLs were then stained with the following transcription factor antibodies for 30 min at 4°C: RORψt (Q31-378, AF647, BD) and Foxp3 (FJK-16s ...
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bioRxiv - Molecular Biology 2023Quote: ... then at 4 °C for at least 30 min before collection with a flow cytometer (BD Biosciences C6 Acuri). 20-30,000 events were collected in specific PE-A and PE-H gates for further deconvolution or data analysis using FlowJo vX 10.0.7r2 (Tree Star ...
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bioRxiv - Bioengineering 2023Quote: ... was used as cloning strain and grown at 37°C in 2*Peptone Yeast Extract mdia (2*PY, 1.6% tryptone peptone (BD), 1% Bacto™ yeast extract (BD ...
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bioRxiv - Immunology 2024Quote: ... 4°C for 10 min and binding was analyzed by flow cytometer FACS LSRFortessaTM (BD Bioscience, Franklin Lakes, NJ) using Yellow/Green Ex 561 nm Em 586/15 laser for the detection of antibodies ...
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bioRxiv - Microbiology 2024Quote: ... Staphylococcal strains were grown at 37°C in brain heart infusion (BHI) broth or on agar plates (BD Biosciences).
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β-1,6-glucan plays a central role in the structure and remodeling of the bilaminate fungal cell wallbioRxiv - Microbiology 2024Quote: ... at 37°C, 180 rpm, in SD medium (2% glucose, 0.67% yeast nitrogen base with amino acids (YNB) (BD), pH 5.4) ...
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bioRxiv - Genetics 2024Quote: ... Following blocking (5% milk/BSA) membranes were incubated with primary antibody overnight at 4°C (BIP [1:40,000 BD Transduction 610979] ...
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bioRxiv - Neuroscience 2024Quote: ... Antigen retrieval was performed for 10-12 min at 95-99°C with antigen retrieval buffer (BD Pharmingen, #550524). After antigen retrieval ...
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bioRxiv - Neuroscience 2024Quote: ... 0.78 mM EDTA) and incubated for 30 min at 4 °C with FACS buffer containing Fc block (BD Biosciences) and primary antibody mix ...
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bioRxiv - Molecular Biology 2024Quote: ... Single cells were stained with the appropriate antibodies to surface markers at 4°C for 30 minutes in the dark.The following fluorescent dye-labeled antibodies purchased from BD Biosciences ...
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bioRxiv - Microbiology 2024Quote: ... and triplicates of 20 µl were cultured aerobically at 37 °C (LB Agar Lennox, BD Beckton Dickinson and Company). Bacteria were enumerated as the number of colony-forming units (CFU ...
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bioRxiv - Microbiology 2024Quote: ... Bacteria were grown to mid-exponential phase at 37 C at 5% CO2 in Todd Hewitt broth (BD Biosciences) supplemented with 0.5% yeast extract and Oxyrase (Oxyrase ...
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bioRxiv - Immunology 2024Quote: ... at 37°C for 30 min in PBS supplemented with 0.1% BSA followed by streptavidin-PE (5 µg/mL) (BD). When co-staining for Annexin V-FITC (10 µg/mL ...
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bioRxiv - Neuroscience 2024Quote: ... in flotation for 1 h at room temperature and then overnight at 4°C with the mouse antibody that recognizes protein GM130 (Cat# A-610822, RRID:AB_398141, BD Biosciences), followed by incubation for 2 h at room temperature with Alexa Fluor 546-conjugated goat anti-mouse secondary antibodies (Cat#A-11030 ...
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bioRxiv - Neuroscience 2024Quote: The full 750-800µL suspension was loaded at 4°C into a FACSAria™ Fusion flow cytometer (BD Biosciences) with a 70 µm nostril running regular 1X PBS at the Flow Cytometry Core Facility of the Molecular Biology Department at Princeton ...
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bioRxiv - Cell Biology 2024Quote: ... for 1 hour and then exposed overnight at 4°C to the primary antibody (ZO-1, 610966, BD transduction). Following three washes in PBS ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were blocked in 5% w/v skim milk in TBS with 0.1% v/v Tween and immunoblotted for cytochrome c (mouse monoclonal antibody, clone 7H8.2C12, #556433, BD Biosciences) or HSP60 (rabbit polyclonal antibody ...
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bioRxiv - Plant Biology 2020Quote: 10-fold serial dilutions (OD600=1, 10-1, 10-2, 10-3) of yeast sample harbouring a DNA-binding domain (BD)-AtCIPK16 and either an activation domain (AD)-AtCBLs or an empty vector (AD ...
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bioRxiv - Genetics 2021Quote: ... and of the 12 mutant strains (4 reporter genes x 3 mutations) was quantified using a Sony MA-900 flow cytometer (the BD Accuri C6 instrument used for other fluorescence assays was not available due to Covid-19 shutdown ...