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Citations for Becton, Dickinson and Company :
851 - 900 of 7615 citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade C 16055 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... and anti-cytochrome c (BD Biosciences #556432).
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bioRxiv - Immunology 2020Quote: ... those to cytochrome c were from BD Pharmingen ...
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bioRxiv - Immunology 2020Quote: ... at 4°C: hCD45-BUV805 (HI30, BD), hCD3-PECyn7 (SK7 ...
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bioRxiv - Cell Biology 2023Quote: ... and cytochrome c (mouse monoclonal, BD-556432) were obtained from BD Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: ... c-Kit PE-CF594 2B8 (BD Biosciences) (1:100) ...
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bioRxiv - Neuroscience 2024Quote: ... αSyn (C-42) (BD Transduction Labs; 610786), β-actin (Sigma-Aldrich ...
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bioRxiv - Systems Biology 2019Quote: ... We therefore identified the brain development protein-protein interaction network (BD-PPI network) for the 22q11.2 locus (i.e. ...
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bioRxiv - Neuroscience 2020Quote: H4 protein extracts were analyzed by western-blotting probing for aSyn (anti-a-synuclein dilution 1:1000; BD Transduction Laboratories™ ...
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bioRxiv - Neuroscience 2019Quote: ... the primary neurons coverslips were incubated with the primary antibodies: microtubule-associated protein 2B (MAP2B; 1:200; BD Transduction Laboratories ...
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bioRxiv - Biophysics 2020Quote: ... the sorted cell population was analyzed 1 hour after induction of protein expression by flow cytometry (Instrument: BD FACS-Aria SORP cell sorter ...
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bioRxiv - Cancer Biology 2021Quote: ... 1% penicillin/streptomycin and 10 mM HEPES (pH 7.4) with 25% high-protein Matrigel (product 354248; BD Biosciences)) ...
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bioRxiv - Immunology 2024Quote: ... and BD GolgiPlug Protein Transport Inhibitor (containing brefeldin A; 1 mg/mL; BD Biosciences, Franklin Lakes, NJ, USA). Following this ...
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bioRxiv - Cancer Biology 2022Quote: ... samples were thawed and sandwich ELISA for human or mouse IFN-γ levels was performed using the BD Human IFN-gamma ELISA Set (BD Biosciences, 555142) or BD Mouse IFN-gamma ELISA Set (BD Biosciences ...
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bioRxiv - Genomics 2019Quote: ... fluorescein isothiocyanate (FITC)- mouse anti-human Annexin-V (biolegend; Cat# 640945) and APC-anti-human anti-CD42b (GPIb) monoclonal antibody (BD Pharmingen; Cat# 551061). The calcein AM blue was used to identify live platelet sized events ...
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bioRxiv - Cell Biology 2024Quote: ... The single cell suspension obtained from human muscle was stained with a cocktail containing PE-Cy5 anti-human CD45 (BD Pharmingen, cat#555484), Percp-Cy5.5 anti-human CD31 (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... The antibodies used for stimulation were anti-human CD3 (clone UCHT1, plate bound) and anti-human CD28 (clone 28.2, BD Biosciences, San Jose, CA) at 1 μg/mL ...
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bioRxiv - Microbiology 2022Quote: ... The cells were collected to detect virus-infected cells via flow cytometry by using a BD LSR II flow cytometer (BD Biosciences, San Jose, CA)) at 48-h post-infection [45] ...
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bioRxiv - Cancer Biology 2021Quote: ... The antibodies used for the flow cytometry analysis of the cell surface markers were: anti-human CD11b (740965, BD OptiBuild™, 1:100), anti-human CD14 (11-0149-42 ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were incubated with a fluorescein isothiocyanate (FITC)-conjugated mouse anti-human CD31 antibody (1:50; BD Pharmingen, San Diego, CA, USA) for 30 min at room temperature followed by 3 washes with phosphate buffered saline (PBS) ...
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bioRxiv - Immunology 2021Quote: NK cells and cancer cells were cocultured at 10:1 ratio and supplemented with 20ul of PE mouse anti-human CD107a antibody (BD Pharmingen™, #555801) in a total volume of 220ul in a 96 well plate at 37C incubator for 90 minutes ...
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bioRxiv - Immunology 2020Quote: ... Cells were incubated for 45 min with the indicated antibodies: AlexaFluor® 647 anti-human CD107a antibody (diluted at 1/100, clone H4A3; BD Pharmingen TM), anti-human Perforin (10µg/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... Human platelets in the obtained blood were identified using APC-labeled polyclonal rabbit anti-human (h) CD41 and/or CD42b (BD Pharmingen, 1:100) and fluorescein isothiocyanate (FITC)-labeled polyclonal rabbit anti-mouse (m ...
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bioRxiv - Cancer Biology 2023Quote: ... 200 μL per 1x10e6 cells) with human IgG-Fc block (1:100) followed by the incubation with streptavidin-magnetic bead particles (BD Biosciences, Ref#557812) at 50 μL per 1x10e6 cells ...
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bioRxiv - Immunology 2021Quote: ... for 5 minutes prior to staining at 4°C in the dark using the antibodies listed in supplementary table 1 and analysed using an LSR Fortessa cytometer (BD Biosciences) and FlowJo software (BD) ...
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bioRxiv - Immunology 2021Quote: ... and surface stained for 30 mins at 4°C with 50 μL/well anti-mouse CD3 APC-CY7 (clone 17A2, 1:400, BD Biosciences), anti-mouse CD4 BV510 (clone RM4-5 ...
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Genetically engineered microglia-like cells have therapeutic potential for neurodegenerative diseasebioRxiv - Neuroscience 2021Quote: ... and with hematopoietic stem cell markers APC-conjugated anti-CD117 (c-Kit) and PE-conjugated Ly6A/E (Sca-1) (BD Biosciences). Lineage negative cells were transduced with a lentiviral vector in a cell incubator at 2 ×106 cells/mL in either StemSpan SFEM II (STEMCELL Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated overnight at 4°C with the astrocyte (mouse anti-GFAP; 1:1000, BD Biosciences, San Diego, CA, USA) and microglia (rat anti-CD11b ...
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bioRxiv - Immunology 2019Quote: ... We then double sorted lineage depleted cells for an HSC-enriched population (Lin- Sca-1+c-Kit+CD34+-CD135-) into 2% FBS in HBSS using fluorescent activated cell sorting (FACS) (BD Aria). Recipient mice ...
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bioRxiv - Neuroscience 2020Quote: ... The pellet was recovered and sequentially incubated with anti-mouse CD16/CD32 (1:250, BD Pharmingen; for 15min at 4°C) followed by rat anti-CD11b and PerCP-Cy5.5 rat anti-mouse CD45 (1:200 ...
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bioRxiv - Neuroscience 2020Quote: ... followed by rat anti-CD11b and PerCP-Cy5.5 rat anti-mouse CD45 (1:200, BD Pharmingen; for 30min at 4°C), all in 2% BSA ...
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bioRxiv - Cancer Biology 2020Quote: ... Discs were then washed three times for 10 minutes each in 0.1% PBST and incubated overnight at 4°C in mouse anti-BrdU primary antibody (B44) (BD, 1:50). The next day ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were then incubated for 72 h at 4°C in mouse anti-PKCδ primary antibody (1:1000, BD Biosciences, 610397) in blocking solution containing 1.5% NGS ...
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bioRxiv - Microbiology 2020Quote: ... during 15 min at 4 °C in MACS buffer followed by incubation with anti-CD45 1:100 (clone 30F-11, BD biosciences), anti-EpCAM 1:100 (clone G8.8 ...
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bioRxiv - Immunology 2022Quote: ... and the remaining cells were incubated for 5-6 hrs at 37°C in the presence of 1 uM of the indicated peptide and brefeldin A (GolgiPlug; BD Pharmingen). Surface staining was done overnight at 4 degrees ...
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bioRxiv - Microbiology 2022Quote: ... cells were fixed in 1% (vol/vol) formaldehyde for 30 min at 4°C and analyzed on an LSRFortessa (BD Biosciences) flow cytometer ...
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bioRxiv - Immunology 2021Quote: ... or GFP-Nb-Cy5.5 (∼51 nM) in 1% FPS/PBS at 4°C for 20 min and subsequently analyzed on a LSR-II cytometer (BD biosciences).
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bioRxiv - Cancer Biology 2020Quote: ... cells were stimulated with 100 ng/ml phorbol 12-myristate 13-acetate (PMA) plus 1 µg/ml ionomycin for 4 h at 37°C in the presence of GolgiPlug/GolgiStop (BD Pharmigen). Cells were stained for surface molecules as described above ...
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bioRxiv - Cell Biology 2019Quote: ... 1-2×106 cells were incubated for 15 min at +4°C with blocking buffer (1xPBS, 1% BSA, 2mM EDTA) containing 5mg/ml α-CD16/CD32 (2.4G2, BD Biosciences). For surface staining ...
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bioRxiv - Cancer Biology 2019Quote: Approximately 0.5-1 × 106 cells were stained for 15-30 minutes at 4°C in 96-well U-bottom plates (BD Biosciences) with directly conjugated antibodies (Table S8) ...
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bioRxiv - Immunology 2021Quote: ... cells were previously stimulated with 100 ng/ml of PMA and 1 μg/ml of Ionomycin for 1h at 37°C and then incubated in GolgiStop and GolgiPlug (both from BD PharMingen) for 2 h at 37°C ...
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bioRxiv - Cell Biology 2020Quote: The following monoclonal antibodies (Abs) were used for flow cytometry and cell sorting: anti-c-Kit (2B8, BD Biosciences, 1:100), -Sca-1 (E13-161.7 ...
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bioRxiv - Microbiology 2020Quote: ... Footpads were digested with 4 mg ml−1 collagenase D for 2 h at 37°C before dissociation through 70 μm cell strainers (BD Biosciences). Homogenates were resuspended in HOMEM supplemented with 20% FCS and serial dilutions (2-fold ...
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bioRxiv - Microbiology 2021Quote: ... Escherichia coli strains were routinely grown aerobically at 37 °C under aerobic conditions with shaking at 1 × g in Luria-Bertani medium (BD, USA), supplemented with 25 μg mL−1 chloramphenicol (Merck ...
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bioRxiv - Immunology 2022Quote: ... were re-stimulated in vitro for 5h at 37°C 5% CO2 in cRPMI supplemented with GolgiStop (1/250; BD bioscience), GolgiPlug (1/500 ...
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bioRxiv - Immunology 2022Quote: ... cultured splenocytes were resuspended in 40 μL FACS buffer containing fluorochrome-conjugated antibodies and incubated 1 hr at 4 °C followed by cell fixation using Cytofix/Cytoperm buffer (BD Bioscience) for 20 min at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... and cells were washed and labeled with the following antibodies for 30 min at 4 °C: rat anti-CD8 APC (1:100, catalog no. 553035; BD Biosciences); rat anti-CD45 PE (1:100 ...
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bioRxiv - Developmental Biology 2024Quote: All cell culture of all species was maintained at 37°C with 5% CO2 and 5% O2 on Matrigel (1:100, BD Corning) coated plates unless otherwise specified ...
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bioRxiv - Developmental Biology 2023Quote: ... and stained with conjugated antibodies for 1 hour on ice: mouse anti-C-peptide antibody conjugated with Alexa Fluor 647 (BD Biosciences) and mouse anti-glucagon antibody conjugated with Bv421 (BD Biosciences) ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibody incubation was overnight at 4 degrees C with the following antibodies at 1:100 dilution in PBS + 3% BSA: CD24-FITC (clone ML5, BD Bioscience), CD271-APC (clone C40-1457 ...
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bioRxiv - Immunology 2023Quote: ... single cell suspensions were incubated with 1 µg/ml SIINFEKL peptides in complete RPMI medium for 1 h at 37°C followed by brefeldin A (Golgiplug; BD Pharmingen) addition and further 5 h incubation ...