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Citations for Becton, Dickinson and Company :
851 - 900 of 1194 citations for Dig 6C SAH 1a b c since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: Bacteroides strains were grown anaerobically for 24 hours at 37 °C in 500μl of brain heart infusion broth (BD Biosciences) supplemented with 5 μg/ml hemin and 0.5 μg/ml vitamin K (BHI-S ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by stimulation for the appropriate time at 37°C with pre-warmed anti-hamster IgG1 antibody (5µg ml-1; G94-56, BD Pharmingen). Ex vivo expansion of B cells was performed using recombinant murine IL-4 (100 u ml-1 ...
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bioRxiv - Neuroscience 2024Quote: ... the slides were incubated overnight at 4°C with primary antibodies: Mouse monoclonal anti-BrdU (1:100, BD Biosciences, 347580) and Rat monoclonal anti-BrdU (1:100 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... for 5 min at 4 °C and immunostained with CD11b-AlexaFluor 647 (M1/70; 1:1000; Cat#557686, BD Biosciences) and CD45-PE (1:1000 ...
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bioRxiv - Immunology 2024Quote: ... Samples were stained with mAbs (30 min at 4°C in the dark) and erythrocytes were lysed (when applicable) using a FACS lysing solution (BD). Isotype-matched nonreactive fluorochrome-conjugated mAbs were used as controls ...
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bioRxiv - Immunology 2024Quote: ... a CFU assay was performed using 10 µl of C-media plated on agar plate (PIA medium, BD, MD USA), incubated at 37°C overnight and colonies were counted.
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bioRxiv - Cancer Biology 2024Quote: ... Samples were then incubated during 32min at 37°C with the following primary antibodies: Ki67 (BD Biosciences 550609, 1:500), CD8a (Abcam EPR21769 ...
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bioRxiv - Neuroscience 2024Quote: ... Pellets were lysed by rotating at 4 °C for 15 minutes after passing through a 26 G needle (BD Biosciences). Tissue suspensions were sonicated on a bioruptor (Diagenode ...
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bioRxiv - Cancer Biology 2024Quote: ... UK), mouse monoclonal PARP (1:1000, #556494, clone 4C10-5) and mouse monoclonal cytochrome-c (1:1000, #556433) from BD Pharmingen (Heidelberg ...
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bioRxiv - Cell Biology 2024Quote: ... albicans were grown as standard overnight cultures at 30°C and 200 rpm in YPD broth (1 % [w/v] yeast extract [BD Difco] ...
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bioRxiv - Cell Biology 2024Quote: ... electro-competent or chemo-competent Escherichia coli strains were grown at 37°C in Luria-Bertani medium (1 % [w/v] tryptone [BD Difco] ...
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bioRxiv - Cancer Biology 2024Quote: ... washed twice with cold CSM and stained with antibodies against vimentin (D21H3 (CST)) and intra-nuclear markers (Histone H3, c-PARP (F21-852 (BD)) for 1 h at room temperature on a shaker ...
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bioRxiv - Developmental Biology 2024Quote: ... samples were resuspended into 300 μL of wash buffer and stored at 4°C for analysis (BD Fortessa X-20). Data analysis was carried out using Flow Jo software ...
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bioRxiv - Cancer Biology 2021Quote: ... for 30 minutes at 4°C and consecutively sorted using a SONY SH800S or a FACS Aria Cell Sorter (BD Biosciences) into NCAM1- and NCAM+ populations ...
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bioRxiv - Cancer Biology 2021Quote: ... for 30 minutes at 4°C and sorted into 384-wells SORTseq plates using a FACS Aria Cell Sorter (BD Biosciences) and directly stored at −80°C until further processing.
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bioRxiv - Microbiology 2020Quote: ... washed with cold PBS by centrifugation (5 min, 550g at 4°C) and analysed with a FACS BD AccuriTM C6 (BD Biosciences) instrument ...
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bioRxiv - Microbiology 2020Quote: ... cells were stored at 4°C until staining with combinations of fluorochrome-conjugated monoclonal antibodies including: anti-CD3 (SP34-2: Pacific Blue; BD Biosciences), anti-CD4 (L200 ...
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bioRxiv - Immunology 2021Quote: ... for 5 minutes prior to staining at 4°C in the dark using the antibodies listed in supplementary table 1 and analysed using an LSR Fortessa cytometer (BD Biosciences) and FlowJo software (BD) ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were incubated for 30 min in the dark at 4°C after adding relevant antibodies (BD biosciences, Franklin Lakes, NJ). A FACSCalibur system (BD biosciences ...
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bioRxiv - Immunology 2021Quote: ... and surface stained for 30 mins at 4°C with 50 μL/well anti-mouse CD3 APC-CY7 (clone 17A2, 1:400, BD Biosciences), anti-mouse CD4 BV510 (clone RM4-5 ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed twice and incubated overnight at 4°C with the fluorochrome-conjugated antibodies and unconjugated rat and mouse IgG in Perm/Wash solution (BD Biosciences), which was followed by additional 5 min incubation in Perm/Wash solution without antibodies ...
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bioRxiv - Cell Biology 2022Quote: Cells harvested at different time points of reprogramming were stained in FACS buffer for 30 min at 4°C and washed with FACS buffer prior to acquisition with LSR Fortessa (BD Biosciences) cytometer ...
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Genetically engineered microglia-like cells have therapeutic potential for neurodegenerative diseasebioRxiv - Neuroscience 2021Quote: ... and with hematopoietic stem cell markers APC-conjugated anti-CD117 (c-Kit) and PE-conjugated Ly6A/E (Sca-1) (BD Biosciences). Lineage negative cells were transduced with a lentiviral vector in a cell incubator at 2 ×106 cells/mL in either StemSpan SFEM II (STEMCELL Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated overnight at 4°C with the astrocyte (mouse anti-GFAP; 1:1000, BD Biosciences, San Diego, CA, USA) and microglia (rat anti-CD11b ...
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bioRxiv - Molecular Biology 2020Quote: ... High sensitivity C-reactive protein (CRP) was measured in serum: blood was collected into a silica and gel containing tube (BD Vacutainer).
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bioRxiv - Molecular Biology 2020Quote: ... resuspended in cold phosphate-buffered saline (PBS) containing 3% FBS and stored at 4°C until analysis using a LSRII flow cytometer (BD Biosciences). FlowJo software v10 (Treestar ...
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bioRxiv - Cell Biology 2022Quote: ... was directly added to the cells and incubated for 30 mins at 37°C and analyzed using a Fortessa flow cytometer (BD Biosciences) at the Imaging and Flow cytometry Core ...
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bioRxiv - Genomics 2021Quote: ... tissues were blocked (3% bovine serum albumin in 5% normal goat sera and 0.1% Triton) and incubated overnight at 4°C with primary antibodies: CD31 (Neomarkers, RB-10333-P0) or CDH1 (BD Biosciences, 6315829). Slides were washed and incubated with Cy3-goat-anti-mouse or anti-rabbit-conjugated secondary antibodies (Jackson Immunoresearch Laboratories ...
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bioRxiv - Immunology 2021Quote: Cultured T cells were stimulated with the cognate SARS-CoV-2 OPPs (ORF1ab-C, ORF3a, N, S, nsp12) and incubated for 4 hours in the presence of Golgiplug (BD Biosciences). Following stimulation ...
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bioRxiv - Cancer Biology 2020Quote: Tumor cells were implanted subcutaneously in the flank of BALB/c mice using 5 × 106 cells (WEHI-164) with 0.5 ml 29G insulin syringes (MicroFine™+, BD medical). Tumor volume was measured with a caliper and volume was calculated using the formula ...
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bioRxiv - Immunology 2021Quote: ... and stained for 20 minutes at 4°C with 50 μl of antibody cocktail containing subset-specific antibodies (Supplementary Table 2) diluted in Brilliant Stain Buffer (BD Biosciences). Cells were washed in FACS buffer and fixed for 20 minutes at 4°C in 100 μl of Fixation Buffer (BioLegend).
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bioRxiv - Microbiology 2021Quote: ... neoformans strains were grown at 30°C in yeast extract-peptone-dextrose (YPD) agar or liquid medium (BD Biosciences, Sparks, MD). S ...
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bioRxiv - Bioengineering 2020Quote: Cell surface staining was performed at 4 °C for 30 min and was analysed using a FACSCalibur flow cytometer (BD Biosciences) and CellQuest Software (BD Biosciences) ...
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bioRxiv - Bioengineering 2020Quote: Cell-surface staining was performed for 45 min at 4 °C and was analyzed using a FACSCalibur flow cytometer (BD Biosciences). A minimum of 1 × 104 events per sample were examined.
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bioRxiv - Neuroscience 2020Quote: ... The pellet was recovered and sequentially incubated with anti-mouse CD16/CD32 (1:250, BD Pharmingen; for 15min at 4°C) followed by rat anti-CD11b and PerCP-Cy5.5 rat anti-mouse CD45 (1:200 ...
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bioRxiv - Neuroscience 2020Quote: ... followed by rat anti-CD11b and PerCP-Cy5.5 rat anti-mouse CD45 (1:200, BD Pharmingen; for 30min at 4°C), all in 2% BSA ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated overnight at 4°C with IFN-γ APC (1:25, Clone XMG1.2, Biolegends) and IL-17 PE (1:50, Clone TC11-18H10, BD Pharmingen) in Perm/Wash buffer ...
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bioRxiv - Neuroscience 2020Quote: ... cells were incubated for 1 hr at 4°C with extracellular domain anti-GluN1 antibody (BD biosciences; 2 μg ml−1) followed by two washes with ice cold phosphate buffered saline (PBS ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were then washed twice 5 min in Tris buffer saline containing 0.01% Triton X-100 (TBST) and incubated with the primary antibody over night at 4°C (human and mouse a-Syn: 610786 BD Biosciences). After incubation with the first antibody ...
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bioRxiv - Cancer Biology 2020Quote: ... Discs were then washed three times for 10 minutes each in 0.1% PBST and incubated overnight at 4°C in mouse anti-BrdU primary antibody (B44) (BD, 1:50). The next day ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were again pelleted at 500xg for 5 min at 4°C then resuspended in Cytofix/Cytoperm reagent (BD Biosciences) and incubated on ice for 30 min Following fixation/permeabilization ...
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bioRxiv - Cell Biology 2021Quote: ... Coverslips were incubated for 25 mins at 37°C 5% CO2 and then fixed and permeabilized with CytoFix/CytoPerm (BD Biosciences) at room temperature for 20 mins ...
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bioRxiv - Microbiology 2020Quote: ... lymphocytes were cultured in 96-well dishes at 37°C for 5-6 h in the presence of 2 μM peptide pool and brefeldin A (BD Biosciences). Cells were then labeled for cell-surface markers ...
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bioRxiv - Microbiology 2021Quote: ... Isolates were resuscitated from cryo stocks preserved at - 80°C by streaking a loopful of each cryo stock onto brain heart infusion (BHI) agar (BD Difco) and incubating a streaked plate for 15-18 h at 32ºC ...
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bioRxiv - Microbiology 2020Quote: Candida strains (Supplementary Table S1) were routinely grown at 30°C in yeast peptone dextrose (YPD) media (2% peptone (BD Bacto), 2% D-glucose (Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... during 15 min at 4 °C in MACS buffer followed by incubation with anti-CD45 1:100 (clone 30F-11, BD biosciences), anti-EpCAM 1:100 (clone G8.8 ...
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bioRxiv - Immunology 2022Quote: Shigella flexneri 2a WT strain 2457T and avirulent plasmid-curated strain BS103 were grown from frozen stocks (−80°C) on Tryptic Soy Agar (TSA) (Difco BD, USA) supplemented with 0.01% Congo Red dye (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... For measurements of CXCL13 the cells were cultured for 5 hours at 37°C in RPMI supplemented with 10% human AB serum and GolgiStop (1/1500; BD Biosciences). For cytokine measurements the cells were plated in the presence of anti-CD3/CD28 (Dynabeads® Human T-activator CD3/CD28 ...
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bioRxiv - Immunology 2022Quote: ... overlapping peptides (10µg/mL) or media alone for 60 hours at 37 °C and 5% CO2 with proliferation dye (Violet Proliferation Dye, BD Biosciences). Peptides were generated in 15mer format with 8 amino acid overlaps for the ID91 fusion antigen ...
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bioRxiv - Immunology 2022Quote: ... and the remaining cells were incubated for 5-6 hrs at 37°C in the presence of 1 uM of the indicated peptide and brefeldin A (GolgiPlug; BD Pharmingen). Surface staining was done overnight at 4 degrees ...