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Citations for Becton, Dickinson and Company :
851 - 900 of 7751 citations for 4 3 2 Chloroethyl 3 nitrosoureido tetrahydro 2H thiopyran 1 1 dioxide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... 50 μl of PBS-BSA containing 2.5 μl of CD11c APC antibody (clone S-HCL-3) or 5 μl of CD11c BV421 antibody (clone B-ly6 RUO) (BD Biosciences) were added to each tube ...
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bioRxiv - Neuroscience 2024Quote: ... sections were blocked with 3% animal serum in PBS-T for 1h and then incubated with anti-Ly6G (BD Bioscience, #551459) primary antibody overnight at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... cells were sorted to select double fluorescence positive clones (eGFP-Lysenin and mOrange-Galectin-3) using a BD FACSAria III Cell Sorter (BD Biosciences). A polyclonal subpopulation with high expression of eGFP-Lysenin was further selected for expansion.
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bioRxiv - Immunology 2024Quote: Fluorescence activated cell sorting (FACS) and flow cytometry analysis were performed using a 3 marker panel including FVS510 Fixable Viability Stain (BD Biosciences), CD45 FITC (Clone 2D1 ...
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bioRxiv - Immunology 2024Quote: Congenically marked WT or IL-27R-/- OT1 T cells were flow sorted 3 days after subunit vaccination using a FACSAria Fusion (BD Biosciences) and total RNA was isolated using Qiagen RNeasy columns ...
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bioRxiv - Immunology 2024Quote: ... in 75μl of 1xPBS for one hour were seeded on coated coverslips for 3 min at 37°C and fixed using Cytofix/Cytoperm-Fixation/Permeabilization Kit (BD biosciences). Coverslips were then gently washed two times with 1x PBS ...
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bioRxiv - Immunology 2024Quote: ... Flow cytometry was performed on a 3 laser Cytek Aurora Cytometer (Cytek Biosciences, Fremont, CA) and analyzed using FlowJo software (BD Biosciences). All antibodies are from Biolegend ...
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bioRxiv - Immunology 2024Quote: ... and then stained with a surface marker antibody cocktail (Supplemental Table 3) using BD Brilliant Staining Buffer (BD Biosciences, PN 563794) for 30 minutes at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: Cells were analysed on the BD LSR Fortessa X-20 or BD FACSymphony or sorted on the BD FACSAria Fusion 5 or BD FACSAria Fusion 3 (BD Biosciences) at the Peter MacCallum Cancer Centre flow cytometry core facility ...
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bioRxiv - Cancer Biology 2024Quote: ... the animals were sacrificed by isoflurane overdose and blood was collected from the portal vein using 24G needles (BD Microlance 3) into microtubes with clot activator (Microvette 500 CAT-Gel ...
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bioRxiv - Cancer Biology 2020Quote: ... Millennium Science) and PCNA (1:100, Becton Dickinson), cavin3 (1:150, Millennium Science) and Aurora Kinase 1 (1:100, BD Biosciences), cavin3 (1:150 Millennium Science ...
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bioRxiv - Neuroscience 2023Quote: ... Santa Cruz Biotechnology, sc50475; TH, 1:1 000, Pel Freeze Biologicals, Rogers, AR, USA, P40101; a-Syn, 1:1 000, BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... followed by Avidin HRP enzyme (eBioscience; 1/100) and TMB substrate (BD Biosciences; 1/1). To end the reaction ...
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bioRxiv - Immunology 2021Quote: ... and surface stained for 30 mins at 4°C with 50 μL/well anti-mouse CD3 APC-CY7 (clone 17A2, 1:400, BD Biosciences), anti-mouse CD4 BV510 (clone RM4-5 ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated overnight at 4°C with the astrocyte (mouse anti-GFAP; 1:1000, BD Biosciences, San Diego, CA, USA) and microglia (rat anti-CD11b ...
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bioRxiv - Immunology 2021Quote: ... Bulk of primary cells (either from the spleen or the lungs) were then rechallenge in vitro with YAK-1 cells for 4 hours in presence of monensin and brefeldin (BD Bioscience) at the Effector/Target ratio of 5 ...
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bioRxiv - Neuroscience 2020Quote: ... The pellet was recovered and sequentially incubated with anti-mouse CD16/CD32 (1:250, BD Pharmingen; for 15min at 4°C) followed by rat anti-CD11b and PerCP-Cy5.5 rat anti-mouse CD45 (1:200 ...
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bioRxiv - Neuroscience 2020Quote: ... followed by rat anti-CD11b and PerCP-Cy5.5 rat anti-mouse CD45 (1:200, BD Pharmingen; for 30min at 4°C), all in 2% BSA ...
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bioRxiv - Cancer Biology 2020Quote: ... Discs were then washed three times for 10 minutes each in 0.1% PBST and incubated overnight at 4°C in mouse anti-BrdU primary antibody (B44) (BD, 1:50). The next day ...
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bioRxiv - Genomics 2021Quote: ... the suspension was sorted by applying a 4-way purity mode based on the selected DAPI positive nuclei population (1:1000, BD Pharmingen) using forward and side scatter gating strategy (FACS) ...
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bioRxiv - Microbiology 2020Quote: ... during 15 min at 4 °C in MACS buffer followed by incubation with anti-CD45 1:100 (clone 30F-11, BD biosciences), anti-EpCAM 1:100 (clone G8.8 ...
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bioRxiv - Microbiology 2022Quote: ... cells were fixed in 1% (vol/vol) formaldehyde for 30 min at 4°C and analyzed on an LSRFortessa (BD Biosciences) flow cytometer ...
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bioRxiv - Immunology 2021Quote: ... or GFP-Nb-Cy5.5 (∼51 nM) in 1% FPS/PBS at 4°C for 20 min and subsequently analyzed on a LSR-II cytometer (BD biosciences).
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bioRxiv - Cancer Biology 2020Quote: ... cells were stimulated with 100 ng/ml phorbol 12-myristate 13-acetate (PMA) plus 1 µg/ml ionomycin for 4 h at 37°C in the presence of GolgiPlug/GolgiStop (BD Pharmigen). Cells were stained for surface molecules as described above ...
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bioRxiv - Immunology 2020Quote: The cell supernatant was collected after centrifugation of the cells and the secreted IL2 in the diluted cell culture medium (1:4 dilution) was measured using the IL2 Flex set cytometric bead array (CBA) (BD, 558270) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... and CD43-B cells were cultured at a density of 5×105 cells per ml in RPMI medium supplemented with 15% FBS and 25 ng ml−1 of IL-4 (R&D) and anti-CD40 (BD Biosciences). Cultured cells were maintained daily at a density of 0.5-1×106 cells per ml ...
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bioRxiv - Immunology 2023Quote: 50 × 103 effector cells were cultured alone or together with 5 × 103 tumour cells for 4 h at a 10:1 ratio in the presence of anti-CD107a-PE mAb (clone: H4A3; BD Biosciences) and monensin (3 μM ...
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bioRxiv - Cell Biology 2023Quote: Both WT and CD13KO mice of both genders were intraperitoneally injected with 1 mL 4% Brewers Thioglycollate (TG) solution (BD; 211716). After 48 hours mice were then euthanized via CO2 and cervical dislocation and injected with 10 mL cold PBS into the peritoneal cavity to collect peritoneal macrophages ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed two times with 150 µL PBS (centrifuged 5 min at 400 × g at 4 °C) and resuspended in 50 µL Fc-block (1:50, Purified Rat Anti-Mouse CD16/CD32, BD, #553142) diluted in FACS buffer (1x PBS/2% FBS/2mM ...
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bioRxiv - Immunology 2023Quote: ... Effector cells were incubated with K562 and Nalm6 variants for 4 hours at a ratio of 5:1 with addition of Brefeldin A (GolgiPlug, BD Biosciences). Next ...
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bioRxiv - Cell Biology 2022Quote: Freshly dissected LW whole mount preparations from 6 wild-type and 4 Lrp2-/267 mice were incubated with rat anti-CD24 conjugated with Phycoerythrin (PE; 1:100; BD Pharmingen) in Neurobasal medium (Gibco ...
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bioRxiv - Immunology 2022Quote: ... cultured splenocytes were resuspended in 40 μL FACS buffer containing fluorochrome-conjugated antibodies and incubated 1 hr at 4 °C followed by cell fixation using Cytofix/Cytoperm buffer (BD Bioscience) for 20 min at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... and cells were washed and labeled with the following antibodies for 30 min at 4 °C: rat anti-CD8 APC (1:100, catalog no. 553035; BD Biosciences); rat anti-CD45 PE (1:100 ...
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bioRxiv - Physiology 2023Quote: ... cells were incubated at 4°C for 30 min with APC-conjugated rat anti-mouse CD31 (1:200, BD Pharmigen, 551262). DAPI (5 mg/ml ...
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bioRxiv - Immunology 2024Quote: ... Tissues were then harvested and fixed for 24 hours in 1ml fixative buffer (BD Cytofix/Cytoperm prepared 1:4 buffered to PBS; BD Biosciences) at 4°C on a slow shaker ...
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bioRxiv - Cell Biology 2024Quote: ... Tissue and cell slides were then blocked with 1% BSA and 10% normal goat serum in PBS at room temperature for 1 hour followed by overnight incubation at 4°C with primary antibodies (1:50 dilution) against human HIF1α (cat # 610958, BD Biosciences), rat HIF1α (cat # NB100-105 ...
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bioRxiv - Cell Biology 2020Quote: Primary human bronchial epithelial cells (Passage 1 or 2) were expanded on flasks coated with collagen I (BD Biocoat) in bronchial epithelial growth medium (BEGM) ...
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bioRxiv - Cell Biology 2022Quote: ... Fixed cells were incubated with either conformation specific BAK antibody (Clone# G317-2, 1:100) (Cat# 556382, BD Pharmingen) followed by phycoerythrin (PE)-conjugated anti-mouse antibody (1:200 ...
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bioRxiv - Microbiology 2021Quote: ... Intracellular staining was performed using rat anti-mouse IFN-γ-PECy7 (clone XMG1-2, 1:300, BD cat#557649), rat anti-mouse IL-2-PE (clone JES6-5H4 ...
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bioRxiv - Immunology 2020Quote: ... T cells and DC were stained with anti-syndecan-1 (rat IgG2Aκ monoclonal antibody, clone 281-2, BD Biosciences) or isotype control (rat IgG2Aκ ...
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bioRxiv - Immunology 2020Quote: PBMCs (1−2×106) were stimulated with epitope-specific peptides (5 μM) and anti-CD28 mAb (0.5 μg/mL, BD) and expanded for 14 days in complete RPMI culture medium containing rIL2 (20 IU/mL ...
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bioRxiv - Immunology 2023Quote: ... Using insulin syringes with a 13 mm long (1/2”) and 27G thick needle (BD, catalog 326716 NJ, USA), 5×106 of PKH26+ cells in a volume of 200 μL were transferred at the 15-16 days post coitus (dpc) ...
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bioRxiv - Immunology 2024Quote: ... cells were blocked with 2% FBS and 0.2% goat serum containing HBSS including 1:1000 Mouse BD Fc Block™ (Clone 2.4G2, BDBiosciences). For detection of endogenous p65 ...
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bioRxiv - Immunology 2024Quote: ... Cells were blocked with 2% FBS and 0.2% goat serum containing HBSS including 1:1000 Mouse BD Fc Block™ (Clone 2.4G2, BDBiosciences) and stained with cell surface marker detecting fluorophore-conjugated antibodies ...
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bioRxiv - Immunology 2024Quote: ... and blocking with 2% FBS and 0.2% goat serum containing HBSS including 1:1000 Mouse BD Fc Block™ (Clone 2.4G2, BDBiosciences). Intracellular proteins were stained with fluorophore-conjugated antibodies overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: Trial types differing by the magnitude of the difference in rank between items (i.e. distance 1: AB, BA, BC, CB, CD, DC, DE, ED, EF, FE, FG, GF; 2: AC, CA, BD, DB ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... cryopreserved human hepatocytes (Lot BD195: 2-year-old, Hispanic female, 1 × 106 hepatocytes, BD Biosciences, Ann Arbor, MI, USA) were transplanted under anesthesia into 2-to 4-week-old hemizygous cDNA-uPA/SCID mice through the spleen ...
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bioRxiv - Cancer Biology 2023Quote: ... resuspended in surface staining mix containing FACS buffer with 1/2 of final volume of Brilliant Stain Buffer (BD), 50 μg/ml anti-mouse CD16/CD32 (2.4G2 ...
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bioRxiv - Neuroscience 2023Quote: ... Digested suspensions were subsequently triturated by sterilized syringes with 20G 1/2 needle (BD Biosciences, Franklin Lakes, NJ, USA) and washed with DMEM to harvest mononuclear cells ...
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bioRxiv - Cancer Biology 2023Quote: ... 1-2×106 cells from tumors were incubated with Fixable Viability Stain UV440 (BD Bioscience, San Jose, CA, USA) for 15 minutes at room temperature in a 96-well plate ...