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Citations for Becton, Dickinson and Company :
8851 - 8900 of 9716 citations for Anti PIF1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... at 4°C for 5 min before surface staining with a cocktail of the following antibodies for 20 min at 4°C: CD3 V450 (Clone 17A2) (BD Cat. 561389), CD4 BV750 (Clone H129.19 ...
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bioRxiv - Cell Biology 2022Quote: ... Hematopoietic cells were harvested from OP9-DL1 cocultures by forceful pipetting and stained on ice for one hour with the following antibodies: Pacific Blue CD4 (1:500; BD Pharmingen, 558107), BV711 CD8a (1:1000 ...
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bioRxiv - Cell Biology 2019Quote: ... The membranes were then blocked with 5% non-fat milk and probed with the following antibodies: β-catenin (1: 3,000, BD Biosciences, USA), Wnt6 (1 ...
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bioRxiv - Cell Biology 2021Quote: ... were fixed on ice with a solution of 1% paraformaldehyde and incubated with fluorescent antibody to platelet markers CD41a (BD Biosciences #555467), activated integrin αIIbβ3 (BD Biosciences #340507) ...
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bioRxiv - Biochemistry 2019Quote: ... cells were incubated for 1 h with primary antibodies to RAD51 (B-bridge International, #70-001) and to cyclin A (BD Biosciences, # 611268) diluted in 1% BSA ...
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bioRxiv - Molecular Biology 2020Quote: ... 200 μl IP lysate samples from each group were incubated with primary antibodies such as eNOS (610297, BD Biosciences, San Jose, CA), acetyl-lysine (9441 ...
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bioRxiv - Immunology 2019Quote: ... Unfractionated and lineage-depleted (Lin-) cells were labelled with phycoerythrin (PE) conjugated antibodies for CD11b and c-Kit (BD Biosciences, Mississauga, ON), and analyzed by flow cytometry ...
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bioRxiv - Genetics 2020Quote: ... villages of iPSCs or NGN2-induced neurons (at 14 days of differentiation) were immunostained with a monoclonal antibody directed against SMN (BD Biosciences, 610647) and segregated into new ‘sub-villages’ comprising populations of the lowest and highest SMN expressing cells.
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bioRxiv - Neuroscience 2021Quote: ... The cell pellet was then stained with antibodies to microglial cell surface markers (CD11b-PE Cy7, 1:200 Biolegend, # 101216; CD45-APC Cy7, 1:200, BD Biosciences # 103116;) for isolation using the FACSAria™ III cell sorter (Fig 1C) ...
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bioRxiv - Immunology 2020Quote: ... All antigen specific CD8 T cells were phenotypically analyzed by Flow Cytometry after surface staining using monoclonal antibodies for Thy1.1(BD clone OX-7), CD45.1 (Biolegend clone A20) ...
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bioRxiv - Immunology 2021Quote: ... Cells were filtered and cell surface staining was performed using antibodies specific for CD3ε (eBioscience, clone145-2C11) and Vγ5 (BD Pharmingen, clone 536).
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bioRxiv - Immunology 2021Quote: ... We used the following 28 antibodies::fluorophore conjugates and clones to enumerate major immune lineages using the Cytek Aurora: HLA-DR::BUV395 (BD; G46-6), CD8::BUV496 (BD ...
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IL-27R signaling serves as immunological checkpoint for NK cells to promote hepatocellular carcinomabioRxiv - Immunology 2020Quote: ... After washing with 1% BSA-PBS slides were incubated with secondary goat anti-rat and goat anti-rabbit biotinylated antibodies for 30 min at RT followed by 30 min of incubation with streptavidin–HRP (1:500; BD Pharmingen, 554066). For developing DAB substrate (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... Single cells were filtered with a 40 µm cell strainer (Falcon, 352340) and incubated with fluorophore-conjugated antibodies CD45–PE/Cy7 (BD Biosciences, 552848), CD11b– PE/Cy7 (BD Biosciences ...
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bioRxiv - Cell Biology 2022Quote: The following antibodies were obtained commercially and used at the indicated dilutions for immunofluorescence: mouse monoclonal TOM20 (BD Biosciences #612278, 1:1000), TOMM20 (Abcam #ab186734 ...
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bioRxiv - Immunology 2022Quote: ... single-cell suspensions were subjected to osmotic red blood cell lysis and incubated with a combination of antibodies: CD4-BV421 (BD Biosciences #562891), CD8-PeCy7 (BD Biosciences #561097) ...
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bioRxiv - Immunology 2022Quote: ... Intracellular staining was carried out with the following antibodies diluted in BD Perm/Wash buffer: Active-caspase-3-GranzymeB-PE (C92-605, BD, cat #561011), Active-caspase-3-BV650 (C92-605 ...
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bioRxiv - Immunology 2022Quote: ... Then the cells underwent membrane surface staining for 30 minutes at 4°C protected from light with Fixable Viability Dye eFluor506 (1:1000, eBiosience, Cat# 65-0866-14) and the following antibodies: CD3 BUV805 (1:50, BD, Cat# 612895), CD8 BUV496 (1:50 ...
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bioRxiv - Cancer Biology 2024Quote: ... and stained with the following conjugated antibodies (1/100 dilution in PBS) for 15 minutes at 4°C: CD44 (PE, BD Biosciences 555479), EpCAM (FITC ...
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bioRxiv - Cancer Biology 2024Quote: ... and with either CSF-1R blocking antibody or isotype control antibody at 50 ng/mL (cat# NBP1-43363, clone AFS98, Novus Biologicals; IgG K isotype control cat# 554682, BD Pharmingen). After 24 hours incubation ...
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bioRxiv - Cancer Biology 2023Quote: Flow-cytometric analysis of mouse bone marrow cells or MUTZ3 cells was done with specific antibody stainings using mouse CD11B-APC (BD, cat 553311) and mouse Gr-1 FITC (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... Single-cell suspensions were stained with antibodies listed in Table S1 and acquired with a LSR Fortessa flow cytometer (BD Bioscience, US). For cell-associated autofluorescence to be removed in the indicated experiments ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were blocked in 5% milk in 0.2% Tween-20 in TBS for 1 h at room temperature and probed with primary antibodies against SORLA (1:4000; 611680, BD Transduction Labs) and GAPDH (1:10,000 ...
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bioRxiv - Microbiology 2023Quote: ... Capture and detection antibodies for human IL-6 were from the OptEIA set for human IL-6 (Cat# 55220, RRID: AB_2869045; BD Biosciences, Heidelberg, Germany) and capture and detection antibodies for human TNF were from the OptEIA set for human TNF (Cat# 555212 ...
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bioRxiv - Microbiology 2023Quote: ... and capture and detection antibodies for human TNF were from the OptEIA set for human TNF (Cat# 555212, RRID: AB_2869042; BD Biosciences, Heidelberg, Germany). Signals were detected by Streptavidin-PE (Agilent ...
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bioRxiv - Cell Biology 2022Quote: ... labelled with cell hashing antibodies and isolated by FACS (all steps at 4°C): Cell suspensions were incubated with Fc-block (1:50, BD Pharmingen 553142) for 10min and subsequently incubated with mouse CD45 MicroBeads (Miltenyi Biotec ...
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bioRxiv - Bioengineering 2023Quote: ... at 10 × 106 cells/mL followed by incubation with antibodies against human CD44 (APC-conjugated, Clone G44-26, 1:5, BD Biosciences, US) and CD24 (PE-Cy7-conjugated ...
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bioRxiv - Cell Biology 2023Quote: ... T cells with residual target protein expression were depleted by antibody staining and bead pull-down or cell sorting (BD Aria III). T cells were re-suspended in MACS buffer (PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... Tumor cell lines were evaluated for expression of B7-H3 using B7-H3 antibody (clone 7-517, BD or clone FM276, Miltenyi).
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bioRxiv - Cell Biology 2022Quote: ... 100,000 PBMCs that had been dissolved and quantified in each media were stained with antibodies for CD3 (APC-H7, BD Biosciences (BD)) ...
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bioRxiv - Cancer Biology 2022Quote: ... and 5% goat serum) for 1h at RT and then stained with EC specific CD31 antibody (BD biosciences, 550274, 1:25 dilution) or muscle base membrane laminin antibody (Sigma ...
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bioRxiv - Immunology 2023Quote: ... and plated in round-bottom 96-well plates at 1-2 x 106 cells per well in complete RPMI (cRPMI) medium containing CD28 costimulatory antibody (BD Biosciences #553294) and brefeldin A ...
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bioRxiv - Immunology 2023Quote: ... cells were stained using fixable live/dead stain for 20 minutes at room temperature and then stained with surface-expressed antibodies (Table S2) diluted in FACS buffer and 1X Brilliant Stain Buffer (BD Biosciences, USA) for 20 minutes at room temperature ...
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bioRxiv - Genetics 2023Quote: Fluorescently conjugated antibodies for cell surface staining (Supplementary Table 1) were prepared in Brilliant Stain Buffer (Cat. no. 556349, BD Biosciences, Australia) and incubated with cells in a 96 well plate (Cat ...
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bioRxiv - Immunology 2023Quote: The Antibody mix was supplemented at 10 uL with BD Horizon™ Brilliant Stain Buffer Plus (BD Biosciences, Franklin Lakes, NJ, USA) to minimize staining artifacts commonly observed when several BD Horizon Brilliant dyes are used ...
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bioRxiv - Immunology 2023Quote: ... For the negative enrichment we used the same antibodies as for the staining but PE-labeled (Biolegend, Cat. 328109, 334105, 303105, 313203, 343605, 304058, 337004 and 305817; BD, Cat. 561433). After negative enrichment ...
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bioRxiv - Immunology 2024Quote: ... and staining of surface markers with a mixture of conjugated antibodies 1:250 in 2%FBS PBS 1mM EDTA buffer with 10% Brilliant Stain Buffer Plus (BD, Cat#566385) for 30 min on ice ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were then stained at a 1:50 dilution with antibodies to immune cell targets as follows: CD3- FITC (BD Biosciences, 555332), CD8-PE (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed and then stained extracellularly with antibodies (Table S1) diluted in 0.5% FACS buffer and brilliant staining buffer (BD Biosciences, Cat# 563794) for 20 mins at room temp ...
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bioRxiv - Immunology 2024Quote: ... three LNs (for patients ccp2 and ccp3) and two replicates of PBMC were stained with a panel of fluorescent antibodies: CD45-PerCP/Cy5.5 (BD 564105, clone HI30), CD38-PE (BD 555460 ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were incubated with anti-mouse CD32 for 5 min at 4°C to block Fc receptors and stained with fluorochrome conjugated antibodies to CD45R/B220 PE (1:200) (clone RA3-6B2, BD Bioscience #553089), Ter119 PE (1:200 ...
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bioRxiv - Microbiology 2024Quote: ... Unspecific binding of antibodies was blocked by incubating the samples with 3% rat serum in BD staining buffer (BD Biosciences, Heidelberg, Germany) for 5 minutes at RT ...
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bioRxiv - Immunology 2024Quote: ... Content of chemokines / cytokines was quantified after washing and staining with biotinylated detection antibodies and phycoerythrin bound streptavidin by using LSR II (BD Bioscience, Germany). Calculation of each chemokine / cytokine quantity was determined by using standard curves according manufacturer’s instructions.
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bioRxiv - Neuroscience 2024Quote: ... cells were blocked with Superblock blocking buffer in PBS/ 0.1% Triton-X for 1 h and then incubated with the following primary antibodies overnight at 4 °C: α-CD11b (1:100; BD Biosciences) and α-Iba1 (1:200 ...
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bioRxiv - Biophysics 2021Quote: ... then incubated for 10 minutes with Alexa Fluor 647 Mouse Anti-Human CD31 (BD Bioscience, 561654) at 1:20 dilutions in EGM-2 media ...
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bioRxiv - Cell Biology 2020Quote: ... Epithelial cells were also excluded using anti-CD326/Epcam (BV421; Cat#563214; BD; used 1:200), and finally ...
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bioRxiv - Immunology 2021Quote: ... Intracellular cytokines were stained with anti-mouse IFN-γ FITC (clone XMG1.1, 1:200, BD Biosciences), anti-mouse TNF-α BV421 (clone MP6-XT22 ...
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bioRxiv - Cancer Biology 2022Quote: ... or DOX was detected using rat anti-mouse CD31 (dilution 1/100, BD Biosciences, cat. 553370) and Alexa Fluor® 546 goat anti-rat (dilution 1/200 ...
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bioRxiv - Cell Biology 2019Quote: ... PGCLCs at day8 were sorted using FITC Mouse anti-SSEA1 (1:20, Cat.#560127, BD Biosciences) and BV421 Hamster Anti-Mouse CD61 (1:50 ...
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bioRxiv - Immunology 2019Quote: ... and stained for the following murine surface markers: anti-CD45 (clone 30-F11) v450 (BD Biosciences), anti-CD326 (Ep-CAM/ESA ...