Labshake search
Citations for Becton, Dickinson and Company :
751 - 800 of 1993 citations for CD3D&CD3E Heterodimer Human HEK293 Fc Flag&Fc His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
Multiplexed single-cell transcriptomic analysis of normal and impaired lung development in the mousebioRxiv - Cell Biology 2019Quote: ... Cells were incubated in the dark with 2 μl/1×106 cells of CD16/32 antibody (Fc block; BD Biosciences, Mississauga, ON, Canada) for 15 minutes at RT ...
-
bioRxiv - Physiology 2021Quote: ... Following resuspension in PBS and passage through a 70μm strainer, cells were incubated in Fc block solution (Bio Rad, Cat# BUF041A) and PerCP-conjugated anti-CD11b antibody (BD Pharminogen, Cat# 550993). After PBS washes ...
-
bioRxiv - Immunology 2021Quote: ... samples were stained with the relevant combination of surface and intracellular antibodies in combination with Fc blocking antibody CD16/CD32 (BD Pharmingen, Cat. #553141). BV421 anti-MHCII(IA/IE ...
-
bioRxiv - Immunology 2021Quote: ... cell pellets were resuspended in x μL of unlabeled CD16/32 mAb Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™), BD Pharmingen 553141) ...
-
bioRxiv - Immunology 2022Quote: ... blood cells were stained in 50 µl 2% FCS RPMI medium with antibodies against CD11C (APC, clone HC3, BD biosciences, dilution 1/100), CD11b (PercPCy5.5 or Pacific Blue ...
-
bioRxiv - Physiology 2023Quote: ... then cells were washed with 1x PBS and resuspended in FACS buffer and non-specific binding to Fc receptors was blocked by incubation with anti-CD16/32 antibody (BD Pharmingen, Cat# 553141) and the FACS antibodies BV711 conjugated anti-CD11b and Pacific blue conjugated anti-CD45 antibodies (Cat# 101241 ...
-
bioRxiv - Immunology 2023Quote: ... cells were washed 2x in FACS buffer (5% FBS, 2 mM EDTA in PBS) and resuspended with anti-CD16/CD32 Mouse BD FC Block (BD Biosciences, Cat. 553142) in FACS buffer at a concentration of 10 ug/ml for 15 min ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were then incubated at 4°C for 15 min with Fc Block (Rat anti-mouse CD16 and CD32; BD Pharmingen – Cat.553141) and 30 min with the antibody mixes ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cell suspensions were blocked for 5 min at 4 °C with rat anti-mouse CD16/CD32 (#553142, Mouse BD Fc Block, BD Biosciences) in FACS buffer ...
-
bioRxiv - Immunology 2023Quote: Cells were first labelled with Fc block for 10 minutes at 4°C and then the following antibodies added: CD45-PE (BD, 30-F.11), Siglec F-APC (BioLegend ...
-
Regulatory T cells control type 1-driven immunopathology restraining GM-CSF-producing helper T cellsbioRxiv - Immunology 2024Quote: ... cells were restimulated ex vivo for 4-5h at 37 °C in complete RPMI medium (RPMI supplemented with 2mM L-Glutamine and 10% fetal calf serum (FCS)) with GolgiPlug (BD, 1:1,000 dilution), phorbol 12-myristate 13-acetate (50ng/ml ...
-
bioRxiv - Developmental Biology 2021Quote: ... were plated in buffer (PBS with 0.5% bovine serum albumin and 1 mM EDTA) and non-specific Fc binding was blocked via incubation with mouse anti-rat CD32 (BD Biosciences #550271; 1:200) for 30 minutes on ice ...
-
bioRxiv - Immunology 2020Quote: The following rat anti-mouse antibodies were used for leukocyte analysis within each tissue: purified CD16/CD32 Fc blocking antibody (clone 2.4G2; BD Pharmingen™, San Jose, CA), PerCP-Cy™5.5 Ly6G antibody (clone 1A8 ...
-
bioRxiv - Cancer Biology 2022Quote: ... Single cell suspensions containing 3×106 cells/sample were stained using appropriate fluorescent antibody cocktails (Listed in Supplementary Table S1) after Fc blocking and analyzed using flow cytometry (BD FACS Celesta/Novocyte Advanteon). Cells were fixed and permeabilized using intracellular staining reagents (Intracellular Fixation & Permeabilization Buffer Set ...
-
bioRxiv - Immunology 2022Quote: ... and F15 NOD Nfκb1 heterozygote were suspended in biotin-free RPMI containing 0.1% azide and 3% FCS and surface stained in 96-well plates with the 10–3.6 PE (anti-I-Ag7) (BD PharMingen, Franklin Lakes, NJ, USA), which is class II major histocompatibility complex (MHC ...
-
bioRxiv - Cancer Biology 2023Quote: ... APC-eFluor780-conjugated cKit and BV605-conjugated Fc Gamma (CD16/CD32) for 30 minutes followed by fixation and permeabilization using BD Cytofix/Cytoperm (BD Biosciences, San Jose, California). Intracellular staining using FITC-conjugated KI-67 was performed overnight at 4 degrees ...
-
bioRxiv - Microbiology 2023Quote: ... spun for 10 minutes and then resuspended in 50 μL of a 1:200 dilution of Fc block (CD16/CD32, clone 2.4G2, BD Biosciences, cat. 553141, 0.5mg/mL) in FACS buffer (PBS ...
-
bioRxiv - Immunology 2023Quote: ... Cells were then stained with monoclonal antibodies conjugated to fluorescent molecules as listed in Supplemental Table 5 after treatment with Fc Block (BD Biosciences, Franklin Lakes, NJ). Cells were analyzed on either a BD LSR-II or BD LSRFortessa X-20 flow cytometer running BD FACSDiva software or a Cytek Aurora spectral cytometer running SpectroFlo software and analyzed using FlowJo software (TreeStar).
-
bioRxiv - Immunology 2022Quote: Antibodies for flow cytometry of mouse samples were: anti-CD3e (clone 145-2C11 in PECF594 from BD Biosciences Cat# 562286 or in FITC ...
-
bioRxiv - Immunology 2022Quote: ... The following antibodies were used for staining as indicated: CD3e-BUV395 (BD Bioscience: 563565, clone: 145-2C11), CD4-APCeFluor 780 (eBioscience ...
-
bioRxiv - Immunology 2019Quote: ... 96 well plates were coated over night at 4°C with 1µg/mL anti CD3e (BD, #553238) and 2µg/mL anti CD28 (BD ...
-
bioRxiv - Systems Biology 2022Quote: ... we used a more comprehensive antibody panel for sorting: anti-CD3e PE-Cy5 (clone UCHT1, BD Biosciences), anti-CD8a Alexa700 (clone SK1 ...
-
bioRxiv - Bioengineering 2021Quote: ... at 1 × 106 cells/ml in wells coated with anti-mouse CD3e (3 µg/ml, BD Pharmingen).
-
bioRxiv - Immunology 2020Quote: ... before being washed and re-suspended in PBS/2% FCS for analysis on a five-laser flow cytometer (LSR FortessaTM; BD Bioscience, San Jose, CA, USA). The data were analysed with FlowJo software (TreeStar ...
-
bioRxiv - Cancer Biology 2023Quote: ... 5X106 cells per mL were washed with cold PBS and blocked in PBS with Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™, BD Biosciences) for 15 min at 4°C in the dark ...
-
bioRxiv - Cancer Biology 2023Quote: ... cells were washed twice in PBS and incubated with 25 µl of an anti-mouse CD16/CD32-Fc/Block (BD Bioscience, San Jose, CA, USA) 20 minutes on ice in the dark ...
-
bioRxiv - Molecular Biology 2023Quote: ... Cells were incubated with fluorochrome-labeled monoclonal antibodies after blocking the Fc receptor with anti-CD16/32 monoclonal antibodies (BD Pharmingen, San Diego, CA. USA). Data were analyzed with FlowJo or Kaluza software ...
-
bioRxiv - Immunology 2024Quote: ... cell pellets were re-suspended in FACS buffer and pre-incubated with rat anti-mouse CD16/CD32 Fc Block™ (Cat# 553142, clone 2.4G2; BD Bioscience, Franklin Lakes, NJ, USA) for 10 min on ice and subsequently incubated with specific fluorochrome-conjugated monoclonal anti-mouse antibodies shown in Supplementary Table 1 ...
-
bioRxiv - Molecular Biology 2022Quote: ... uncultured splenocytes were incubated with antibodies (anit-CD45 APC/Cy7 [Biolegend, 103116], anti-CD11C FITC [Biolegend 117306], anti-CD3e Percp5.5 [BD Pharmingen ...
-
bioRxiv - Immunology 2023Quote: ... Cells were stained on ice for 30min in FACS buffer with 1:100 dilutions of biotin-conjugated-HA and purified rat anti-mouse CD16/CD32 (Mouse BD Fc Block™, Clone 2.4G2, BD Biosciences), then stained on ice for 30min in FACS buffer with 1:1000 dilution of allophycocyanin (APC)-conjugated streptavidin ...
-
bioRxiv - Immunology 2019Quote: Tumor cells or T cells suspended in FACS staining buffer were stained with fluorochrome-conjugated antibodies against CD3e (SK7, BD) and murine TCRβ (12-5961-82 ...
-
bioRxiv - Bioengineering 2021Quote: ... Cells were activated by seeding in 96-well plates pre-coated with anti-mouse CD3e (1 μg/ml working concentration, Clone: 145-2C11, BD) and anti-mouse CD28 (2 μg/ml working concentration ...
-
bioRxiv - Bioengineering 2021Quote: ... at 2×106 cells/ml in wells coated with anti-mouse CD3e (1 μg/ml, Clone: 145-2C11, BD Pharmingen).
-
bioRxiv - Microbiology 2021Quote: ... the splenocytes were stained with the extracellular markers PE-Cy ™ 7 Hamster Anti-Mouse CD3e (Cat. # 552774, BD Pharmingen), APC-Cy7 Rat Anti-Mouse CD4 (Cat ...
-
bioRxiv - Genomics 2020Quote: ... and a panel of 5 FITC-conjugated lineage antibodies (anti-CD41, anti-CD45R, anti-CD3e, anti-CD11b and anti-Ly-6G/6C, all at 1 μg/ml; BD 553848 ...
-
bioRxiv - Immunology 2022Quote: Round-bottom 96-well plates were coated with 100 μL of PBS containing a mixture of 0.5 mg mL-1 purified NA/LE hamster anti-mouse CD3e (clone: 145-2C11, BD Pharmingen) and 0.5 mg mL-1 anti-mouse CD28 (clone ...
-
bioRxiv - Immunology 2022Quote: ... anti-CD3e-PerCP-Cy5.5 (eBioscience, clone 145-2C11, 1/100) and anti-CD11b-APC (BD, clone M1/70, 1/100), and the DRAG1 line was selected ...
-
bioRxiv - Developmental Biology 2021Quote: Mouse femur single cells were obtained as described above in the flow cytometry section and stained with biotin-conjugated antibodies for Lin (Ter119, Gr-1, B220, CD3e) (BD, 559971) followed by negative selection using streptavidin-coated magnet beads (Miltenyi Biotec ...
-
bioRxiv - Biochemistry 2020Quote: ... Lactate excretion in primary mouse T cells was measured using a YSI-2900 Biochemistry Analyzer after 24 hours of stimulation using anti-CD3e (BD Biosciences 553057) and anti-CD28 (BD Biosciences 553294 ...
-
bioRxiv - Immunology 2020Quote: ... up to 5×106 leukocytes were incubated with 5 μg/mL E6 peptide or 1 μg/mL α-CD3e (clone 145-2C11, BD Biosciences) in the presence of 1:1000 Golgi-plug for 5 hours ...
-
bioRxiv - Synthetic Biology 2021Quote: ... Binding (ACE2+/FLAG+) and non-binding (ACE2-/FLAG+) cells were sorted by FACS (BD FACSAria Fusion or Sony MA800 cytometer ...
-
bioRxiv - Plant Biology 2022Quote: ... The constructs were then co-transformed into yeast AH109 strain and plated on SD selective media and no detectable self-activation was observed (SD -His-Leu, + 2 mM 3-AT or SD -His-Trp, + 40 mM 3-AT (for OsSHR1-BD) or 20 mM 3-AT (for OsSHR2-BD)) ...
-
bioRxiv - Immunology 2024Quote: ... Purified CD4+ cells were cultured in 24-well round-bottom plates (1×106 cells/well) with plate-bound anti-CD3e (2.5 μg/ml, clone 2C11, BD Biosciences, San Diego, CA), soluble anti-CD28 (2 µg/ml ...
-
bioRxiv - Immunology 2022Quote: ... CD69 BV711 (Clone HI.2F3) (BD Cat. 740664), CD103 PE-CF594 (Clone M290 ...
-
bioRxiv - Immunology 2023Quote: ... the ACE2/HEK293 cells were harvested and GFP expression levels determined by BD FACSCelesta™ flow cytometry ...
-
bioRxiv - Immunology 2024Quote: ... Purified naïve CD4+ T cells were stimulated with 2.5 μg/ml of anti-CD3e (#145-2C11, Cytek Biosciences, Fremont, CA) and 1 μg/ml of anti-CD28 (#553294, BD Biosciences, Franklin Lakes, NJ) antibodies in different culture conditions ...
-
bioRxiv - Synthetic Biology 2024Quote: ... Binding (ACE2+/FLAG+) and non-binding (ACE2-/FLAG+) yeast cells were sorted by FACS (BD FACSAria Fusion). Collected cells were immediately checked for purity and resorted if necessary.
-
bioRxiv - Immunology 2023Quote: ... anti-CD8 (as above) and anti-CD69 (HI.2F3, BD Bioscience) or anti-IRF4 (3e4 ...
-
bioRxiv - Cell Biology 2020Quote: ... anti-human CD44 or anti-human CD59 (BD Pharmingen) or 5μg/ml Biotin-Tfn (Sigma) ...
-
An innovative hematopoietic stem cell gene therapy approach benefits CLN1 disease in the mouse modelbioRxiv - Neuroscience 2022Quote: ... mouse Alexa647 anti-human human CD271 (NGF Receptor) (BD Pharmingen). For characterization of lin-purity ...