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Citations for Becton, Dickinson and Company :
7851 - 7900 of 7984 citations for 2 1 ethylpentyl 1 3 dioxan 5 yl laurate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Lipid peroxidation was detected by measuring the fluorescence intensity of BODIPY 581/591 C11 using BD FACS Canto 2 (BD Biosciences). In each case ...
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bioRxiv - Cell Biology 2022Quote: ... Cells expressing both DOR and dCas9 were isolated using fluorescence activated cell sorting (FACS) on a BD FACS Aria 2 (BD Biosciences), serially diluted ...
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bioRxiv - Cancer Biology 2023Quote: C4-2 cells were cultured and lifted from plates by trypsinization then re-suspended in 100 mL of 50% Matrigel (BD Biosciences) and saline mixture followed by subcutaneous injection in two separate groups of castrated and non-castrated athymic nude mice (age at least 6 weeks old) ...
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bioRxiv - Biophysics 2022Quote: ... The FITC ERF values were assigned to both 2 μm and 6 μm beads using a specific lot of FITC-FC Bead (BD Biosciences) as a calibrator with known ERF value ...
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bioRxiv - Microbiology 2023Quote: ... PBMCs were then stained with live/dead and cell surface markers in MACS solution (Miltenyi) supplemented with 2% BSA (Miltenyi) prior to permeabilization with Cytofix/Cytoperm (BD Biosciences) and staining with intracellular markers in Perm/Wash (BD Biosciences) ...
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bioRxiv - Microbiology 2023Quote: ... PBMCs were then fixed in 2% formaldehyde and stored at 4°C until flow cytometry on an LSR II flow cytometer (BD Biosciences). Cell surface markers included CD3 (clone 17A2 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Isolates from the CNRMA in France were subcultured on 2% malt extract agar (Oxoid) and potato dextrose agar (BD Diagnostic Systems) for 5 days at 30 °C ...
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bioRxiv - Cell Biology 2024Quote: ... was added to a final concentration of 2 μg/mL and cells examined by flow cytometry using a FACSLyric flow cytometer (BD Biosciences). For controls (negative ...
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bioRxiv - Immunology 2024Quote: T cells were restimulated with 3 μg/ml plate-bound anti-CD3 and 2 μg/ml soluble anti-CD28 or PMA/Ionomycin in the presence of GolgiPlug protein transport inhibitor (BD Biosciences) for 6 hours ...
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bioRxiv - Genomics 2024Quote: ... After 1-2 weeks, mCherry+ cells were sorted by FACS (Flow Cytometry Core Facility, EPFL) using FACSAriaII or FACSAriaFusion flow cytometers (BD Biosciences) and dispensed as single cells into 384-well plates containing conditioned medium (50:50 fresh 10% FBS ...
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bioRxiv - Immunology 2024Quote: ... Purified B cells were seeded at a density of 2-10 * 103 cells per well in 96-well Microtest U-bottom plates (BD Falcon) and were cultured with αCD40 (150 ng/mL ...
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bioRxiv - Immunology 2024Quote: ... Samples for flow cytometry were fixed in 2% paraformaldehyde solution in PBS and analyzed using a LSRII or A5 flow cytometer (BD Biosciences) and FlowJo software (Tree Star ...
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bioRxiv - Developmental Biology 2024Quote: ... hPSCs were seeded as single cells (2-4×105 cells/ml, see hPSC maintenance) on coated 12-well plates (BD Falcon). Cells were constantly maintained at 37°C in 5% CO2 with 95% air.
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bioRxiv - Molecular Biology 2024Quote: ... cells were resuspended in DPBS containing 2% FCS and 1mM EDTA and immediately analyzed on a FACSCanto II or FACSymphony A1 Cell Analyzer (BD Biosciences). For yH2AX measurements in U2OS cells ...
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bioRxiv - Microbiology 2024Quote: ... The plates were incubated for 2 h at 37 °C before the bacteria were resuspended in a final volume of 100 µl MH broth (BD Biosciences). Serial dilutions in MH broth were carried out and 5 µl of each dilution was spotted in technical duplicates on MH agar plates supplemented with 50 µg/ml ampicillin for calculation of total number of colony forming units (CFUs ...
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bioRxiv - Bioengineering 2024Quote: ... for viability assessment and fixed with 2% paraformaldehyde (30 min incubation) until analysis by flow cytometry using an LSR Fortessa (BD Biosciences). For assessment of NP-associated fluorescence in a fluorescence plate reader ...
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bioRxiv - Immunology 2024Quote: ... Cell culture supernatants were assayed for IL-2 using capture and biotin-labeled detection antibody pairs (BD Biosciences, Franklin Lakes, NJ). Detection was performed with HRP-conjugated streptavidin (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... the cells were fixed with 2% (w/v) paraformaldehyde and stored at 4°C before analysis with FACS Celesta Cell Analyzer (BD Biosciences). Data were analyzed using FlowJo software version X.0.7 (Tree Star ...
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bioRxiv - Microbiology 2024Quote: Splenocytes or lung cells were cultured in presence or absence of 2 μL/mL of cell stimulation cocktail (eBioscience, 00-4975-93) and brefeldin A (BD, 555029) in RPMI complete media for 6 hours at 37°C in a humidified chamber and 5% CO2 ...
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bioRxiv - Biophysics 2024Quote: ... After incubation cell free supernatant was collected and subjected to sandwich ELISA using IL-2 ELISA kit (BD biosciences, CA, USA) as per manufacturer instruction ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatant was discarded and the cells were resuspended in 2 mL red blood cell lysis buffer (1X, BD Biosciences, 555,899), mixed by pipetting up-and- down ...
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bioRxiv - Cancer Biology 2022Quote: ... The co-cultures were incubated for 6 hours at 37°C and 5% CO2 prior to cell staining using the Cytofix/Cytoperm Fixation Kit (BD Biosciences, Cat# 554715/555028).
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bioRxiv - Immunology 2021Quote: ... Single leucocyte suspensions were stimulated with either phorbol 12-myristate 13-acetate (PMA)-ionomycin or 5 µg/ml of MCMV peptide pool (Table S3) for 6 hours with brefeldin A (BD Biosciences, San Jose, CA). After stimulation ...
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bioRxiv - Neuroscience 2020Quote: ... infected mouse brain for flow cytometry were obtained as described.(Posel et al., 2016) Isolated cells were incubated overnight (5 hours for microglia analysis) in GolgiPlug and GolgiStop (BD Bioscience #555029 and #554724) in RPMI media containing HEPES and 10% fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were resuspended in PBS + 5% FBS and analyzed via Fluorescence-Assisted Cytometry (FACs) using a BD LSR Fortessa (BD Biosciences, San Jose, CA). Fluorescence minus one (FMO ...
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bioRxiv - Microbiology 2021Quote: Lorikeet intestinal contents collected at necropsy were plated on reduced Trypticase Soy Agar (TSA II) with 5% sheep blood (BD BBL, Franklin Lakes, NJ) and incubated anaerobically (5% CO2 ...
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bioRxiv - Immunology 2020Quote: Single cell preparations were cultured in complete media (106 cells /ml) for 72h in anti-CD3/anti-CD28 (5 μg/mL; BD Biosciences, San Jose, USA) pre-coated 96-flat-well plates (BD Biosciences) ...
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bioRxiv - Genomics 2020Quote: ... at a concentration of 100μg/ml starting 5 days after transfection for the following 7-9 days before single cell sorted using BD FACSAria II (BD Bioscience, San Jose, CA) for clonal cell line generation ...
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bioRxiv - Immunology 2021Quote: ... Assay plates were incubated 24-48 h at 37⍰°C in a 5% CO2 incubator and developed using BD ELISpot AEC substrate set (BD Biosciences, San Diego, CA). Spots were counted and analyzed using an ELISpot reader and ImmunoSpot®software v6 (Cellular Technology ...
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bioRxiv - Microbiology 2021Quote: ... This was done by treating both the PDMS and glass surface with 5-10 sweeps of a portable laboratory corona device (model BD-20ACV, Electro-Technic Products). After treatment the chip was placed carefully onto the glass slide and gently tapped to facilitate full contact between the PDMS and glass surface ...
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bioRxiv - Immunology 2021Quote: ... The stimulation was performed by incubation for 6 h at 37°C and 5% CO2 in the presence of Protein Transport Inhibitor Cocktail (brefeldin A) (BD Biosciences, San Jose, CA) at a final concentration of 1:1000 ...
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bioRxiv - Immunology 2023Quote: ... Cells were then stained with monoclonal antibodies conjugated to fluorescent molecules as listed in Supplemental Table 5 after treatment with Fc Block (BD Biosciences, Franklin Lakes, NJ). Cells were analyzed on either a BD LSR-II or BD LSRFortessa X-20 flow cytometer running BD FACSDiva software or a Cytek Aurora spectral cytometer running SpectroFlo software and analyzed using FlowJo software (TreeStar).
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bioRxiv - Molecular Biology 2022Quote: ... cells were washed with ice-cold DPBS plus 5 mM EDTA and stained with annexin V and PI (BD Biosciences, Franklin Lakes, NJ, USA) for 15 min at RT ...
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bioRxiv - Immunology 2022Quote: ... the frozen bacterial stock was thawed in 37°C water bath and cultured at 37°C in 5% CO2 in Todd Hewitt broth (BD Biosciences, San Jose, CA) to mid-logarithmic phase ...
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bioRxiv - Developmental Biology 2023Quote: ... cells filtered into 5 ml polystyrene tubes and analyzed by flow cytometry using a FACS Caliber flow cytometer and FlowJo software (BD Biosciences, Franklin Lakes, NJ).
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bioRxiv - Genetics 2024Quote: ... the pigs were fasted overnight and 5∼10 mL blood was sampled from the jugular vein using a vacutainer (Cat no. 367986, BD Vacutainer SST™, USA) and transported to the laboratory within 10 min ...
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bioRxiv - Immunology 2024Quote: ... CD8+ T cells were then cultured at 1.106 cells/well in 12 well/ plate that were coated overnight one day earlier with anti-CD3 mAb (clone145-2C11, BD pharmingen, 5 µg/ml) within 2 ml/well of complete medium (RPMI 1640 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were blocked with 5% BSA in PBS and BrdU was then detected with a mouse anti-Brdu primary antibody (BD Biosciences, Clone B44, 347580) and goat anti-mouse AlexaFluor-488 conjugated secondary (1:500 ...
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bioRxiv - Cancer Biology 2021Quote: ... The bottom layer of soft agar was prepared by mixing 2 × growth medium and 1.5% noble agar (BD Biosciences, San Jose, CA) at a 1:1 ratio and the mixture was poured into 6-well plates ...
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bioRxiv - Microbiology 2020Quote: ... Strains were precultured during 48h in 300 μL of Synthetic Defined (SD) medium (Warringer et al., 2011) with some modifications (2% glucose, 0.14% Yeast Nitrogen Base (BD Difco™, U.S.A.), 0.5% ammonium sulfate ...
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bioRxiv - Microbiology 2021Quote: ... the ELISA was performed by coating 96 well plates with 2 µg/mL LRRC15-His (AcroBio, #LR5-H52H3) in coating buffer (BD, #51-2713KC) overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: Streptococcus pneumoniae D39 (serotype 2 clinical isolate) and isogenic mutant strains were cultured in Todd-Hewitt broth (Becton, Dickinson and Company; BD) supplemented with 0.2% yeast extract (BD ...
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bioRxiv - Microbiology 2020Quote: Streptococcus pneumoniae D39 (serotype 2 clinical isolate) and isogenic mutant strains were cultured in Todd-Hewitt broth (Becton, Dickinson and Company; BD) supplemented with 0.2% yeast extract (BD ...
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bioRxiv - Microbiology 2020Quote: ... 50 μL of EDTA-anticoagulated whole blood were stained with the monoclonal antibodies CD3 BV605 (SP34-2, BD Biosciences, San Jose, CA), CD4 PerCP/Cyanine5.5 (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... The hemocytes were collected from thirty to forty shrimps at 2 hours post injection for each sorting which was performed on a FACSMelody cell sorter (BD Biosciences, USA). The fluorescence boundary was set based on detection of the shrimp hemocyte self-fluorescence without VP injection.
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bioRxiv - Immunology 2020Quote: ... IL-2 and IFN-γ concentration secreted in cell supernatant were measured using sandwich ELISA according to manufacturer’s protocol (BD Biosciences, USA). Plates were read on a Varioskan Lux multimode microplate reader (ThermoFisher ...
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bioRxiv - Pathology 2022Quote: ... the samples were washed and resuspended in PBS supplemented with 2% FBS for analysis on a five-laser flow cytometer (BD Biosciences, Fortessa). The data were analysed with FlowJo software (TreeStar).
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bioRxiv - Immunology 2022Quote: ... For stimulation ∼106 lymphocytes plated in medium containing 2% fetal calf serum and 1.5 μl/ml Golgiplug (BD Bioscience; San Jose, CA) were cultured with peptides each present at a final concentration of 2 µg/ml for 5 hr at 37°C in a 5% CO2 incubator ...
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bioRxiv - Immunology 2020Quote: ... 7 and 14) or at 2 time points (days 1and 14) after vaccination into Vacutainer® Heparin tubes (BD Biosciences, Plymouth, U.K.). Blood samples were subjected to immediate preparation and analyses ...
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bioRxiv - Pathology 2020Quote: ... Tomato-positive and -negative cells differed by ∼2 orders of magnitude in tomato fluorescence with excitation of 554nm and emission of 581nm by Influx™ cell sorter (BD Biosciences).